Agricultural and Biological Sciences, Renewable Energy, Sustainability and the Environment, Waste Management and Disposal, Applied Microbiology and Biotechnology
Evaluation of Laboratory-Scale Composting Reactors to Simulate Compost Barn Bedding Dynamics Gabriela Maria Fernandes Fiães, Marcelo Henrique Otenio, Luana de Araújo Coimbra, Caio de Teves Inácio, Leonardo de Souza Ramos Environmental Quality Management, 2026 Efficient management of compost bedding is essential for sustainability and animal welfare in Compost Barns (CBs). This study evaluated laboratory‐scale bioreactors as tools to simulate CB composting dynamics, focusing on temperature control, aeration, and wood shaving supplementation. Three sequential experiments were conducted. Test 1 used a 50:50 mix of shavings and compost bedding for three months. Test 2 operated 1‐week cycles with 100% compost bedding without temperature control. Test 3, also lasting 1 week, applied active temperature regulation at 39°C with 100% bedding. Aeration was set at 15 mL/min in Tests 2 and 3. All experiments included initial and final measurements of pH, total solids (TS), and microbiological indicators. Results indicated that temperature control in Test 3 improved organic matter degradation and suggested enhanced pathogen reduction. These findings demonstrate that laboratory‐scale bioreactors are effective for simulating CB composting and underscore the importance of temperature and aeration management. Future studies should further optimize temperature control and reactor design to enhance microbial activity and compost stabilization. To our knowledge, this is the first study to validate 3‑L laboratory‐scale bioreactors specifically for simulating CB bedding dynamics.
ENERGY RECOVERY FROM THE ANAEROBIC CO-DIGESTION OF RICOTTA CHEESE WHEY AND DAIRY CATTLE MANURE Larice Aparecida Santana, Guilherme Henrique Da Silva, Jailton da Costa Carneiro, Isabela Gomes Da Motta, Henrique Vieira Mendonça, et al. Bioscience Journal, 2026 This study evaluated biogas production, methane concentration and yield, as well as the potential for energy production of anaerobic co-digestion of ricotta cheese whey and dairy cattle manure in 60 L plug flow reactors. Four reactors were operated: three with different proportions of ricotta cheese whey (20, 40, and 80%) and a control reactor containing only dairy cattle manure. The reactors were operated for 106 days, with a hydraulic retention time of 30 days. In all reactors, pH remained near neutrality, and alkalinity stayed above 2,500 mg L⁻¹. Notably, only the reactor with 80% ricotta cheese whey demonstrated more efficient removal of total solids, volatile solids, biochemical oxygen demand, and chemical oxygen demand, with average reductions of 54, 69, 69, and 78%, respectively. However, the reactor operated with 40% ricotta cheese whey produced the highest accumulated biogas volume, surpassing the 80% ricotta cheese whey reactor by 36 L, with a realistic extrapolation of electricity production estimated at 1.67 kWh m-³. Although methane yields were close to theoretical maximum values and methane concentrations showed no significant differences between treatments, the results confirm the effectiveness of anaerobic co-digestion in the treating of agro-industrial waste. The 40% ricotta cheese whey and 60% dairy cattle manure ratio stands as a promising alternative for biogas production.
Biosurfactant Production by Pseudomonas: a Systematic Review Larissa de Souza Araújo, Larice Aparecida Rezende Santana, Marcelo Henrique Otenio, Clerison Wagner Nascimento, Ana Flávia Lawall Werneck Cerqueira, et al. Applied Biochemistry and Biotechnology, 2024
Valorization and Bioremediation of Digestate from Anaerobic Co-Digestion of Giant Reed (Arundo donax L.) and Cattle Wastewater Using Microalgae Guilherme Henrique da Silva, Natália dos Santos Renato, Alisson Carraro Borges, Marcio Arêdes Martins, Alberto José Delgado dos Reis, et al. Sustainability Switzerland, 2024 Anaerobic digestion followed by microalgal cultivation is considered a promising renewable alternative for the production of biomethane with reduced effluent generation, thus lowering the environmental impact. In this arrangement, in addition to generating energy, the microalgae act by potentiating the refinement of the effluents generated via anaerobic digestion (digestates). In this study, the microalga Tetradesmus obliquus was cultivated in photobioreactors with the final digestate resulting from the co-digestion of Arundo donax L. plant biomass and cattle wastewater. The biotechnological route used was efficient, and the biogas production ranged from 50.20 to 94.69 mL gVS−1. The first-order kinetic model with variable dependence (FOMT) provided the best fit for the biogas production data. In the microalgal post-treatment, the removal values ranged from 81.5 to 93.8% for the chemical oxygen demand, 92.0 to 95.3% for NH4+-N, and 41.7 to 83.3% for PO43− after 26 days. The macromolecular composition of the algal biomass reached lipid contents ranging from 33.4 to 42.7%. Thus, the proposed process mediated by microalgae can be considered promising for the bioremediation and recovery of effluents produced by agriculture through the use of microalgal biomass for bioproduct production.
Influence of meteorological parameters on the concentration of heavy metals and etiological agents in sewage sludge samples as a tool for their use in agriculture Periodico Tche Quimica, 2020
Sugarcane growth with cattle biofertilizer and urea Henrique Vieira de Mendonça, Jean Pierre Henry Balbaud Ometto, Wadson Sebastião Duarte da Rocha, Carlos Eugênio Martins, Marcelo Henrique Otenio, et al. Revista Em Agronegocio E Meio Ambiente, 2016
Evaluation of Laboratory‐Scale Composting Reactors to Simulate Compost Barn Bedding Dynamics GMF Fiães, MH Otenio, LA Coimbra, CT Inácio, LS Ramos Environmental Quality Management 35 (3), e70254 , 2026 2026
Granular organic fertilizers from livestock waste via integrated UF-RO and granulation with soil performance evaluation TG Carpanez, LMC Castro, BL Moreira, JBLS Carneiro, EVS Gomes, ... Sustainable Materials and Technologies, e01908 , 2026 2026
Energy recovery from the anaerobic co-digestion of ricotta cheese whey and dairy cattle manure. LAR Santana, GH da Silva, JC CARNEIRO, IGB da Motta, ... 2026
Soil fertilization potential and nutrient dynamics of organic fertilizers derived from sugarcane residues under techno-economic and environmental assessment TG Carpanez, LMC Castro, BL Moreira, JBLS Carneiro, EVS Gomes, ... Chemosphere 389, 144708 , 2025 2025 Citations: 3
Membranes for nutrient recovery from waste and production of organo-mineral fertilizer in the context of circular economy TG Carpanez, VR Moreira, JBG Silva, MH Otenio, MCS Amaral Water Research, 124560 , 2025 2025 Citations: 5
Recycling in livestock: Reuse of corral washing water and reduction of the release of pathogenic microorganisms into the environment J Kassis, TG Carpanez, AM de Melo Junior, HV de Mendonça, MH Otenio, ... Journal of Water Process Engineering 76, 108217 , 2025 2025
Quais os entraves à universalização do acesso às fontes de energia no Brasil? R Gambetta, RC Leitão, MH Otenio, A Kunz ENERGIA LIMPA E ACESSÍVEL, 20 , 2025 2025 Citations: 1
Energy potential of elephant grass broth as biomass for biogas production GH da Silva, N dos Santos Renato, FF Coelho, TP Donato, AJD Dos Reis, ... Scientific Reports 15 (1), 8635 , 2025 2025 Citations: 10
Impact of anaerobic digestion on Escherichia coli resistance to biocides from molecular and phenotypic insights. JA RESENDE, MR ALVES, MF LIMA, MS FONTES, MH OTENIO, ... 2025
Aplicação de biorreatores laboratoriais para simulação de cama de Compost Barn. S ANDRADE, GMF FIÃES, LA COIMBRA, AS GUIMARÃES, MH OTENIO, ... 2025
Avaliação do crescimento de Pseudomonas sp. em permeado de soro de leite. PHG PICOLI, OAB de PAULA, MH OTENIO, EF ARCURI, PB ARCURI, ... 2025
Avanços na biotecnologia de enzimas hidrolíticas industriais: biodigestão anaeróbia como fonte de microrganismos produtores: um estudo de revisão. LAR SANTANA, EMT CLARO, FM FERNANDES, MH OTENIO, ... 2025
Potencial de produção de biogás e biometano a partir da biomassa energética de genótipos de capim-elefante. FF COELHO, GH da SILVA, EHO DIAS, MH OTENIO, JC CARNEIRO, ... 2025
Biosurfactant production by Pseudomonas: a systematic review L de Souza Araújo, LAR Santana, MH Otenio, CW Nascimento, ... Applied Biochemistry and Biotechnology 196 (12), 9049-9063 , 2024 2024 Citations: 9
Valorization and Bioremediation of Digestate from Anaerobic Co-Digestion of Giant Reed ( Arundo donax L.) and Cattle Wastewater Using Microalgae GH Silva, NS Renato, AC Borges, MA Martins, AJD Reis, MH Otenio Sustainability 16 (23), 10328 , 2024 2024 Citations: 3
Potential for nutrients reuse, carbon sequestration, and CO2 emissions reduction in the practice of domestic and industrial wastewater recycling into agricultural soils: A review TG Carpanez, JBG Silva, MH Otenio, MCS Amaral, VR Moreira Journal of Environmental Management 370, 122443 , 2024 2024 Citations: 19
Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security KV Menezes, CE de Souza Duarte, MG Moreira, TJC Moreno, ... Water Research 252, 121192 , 2024 2024 Citations: 5
Biosurfactant production by Pseudomonas: a systematic review A LdS, LR Santana, MH Otenio, CW Nascimento, A Cerqueira, ... 2024
Virulence profile of Candida spp. isolated from an anaerobic biodigester supplied with dairy cattle waste CB Dos Reis, MH Otenio, AM de Melo Júnior, JCM Dornelas, ... Microbial Pathogenesis 187, 106516 , 2024 2024 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure JA Resende, VL Silva, TLR de Oliveira, S de Oliveira Fortunato, ... Bioresource technology 153, 284-291 , 2014 2014 Citations: 142
Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852 MH Otenio, MTL Silva, MLO Marques, JC Roseiro, ED Bidoia Brazilian Journal of Microbiology 36, 258-261 , 2005 2005 Citations: 125
Microalgae-mediated bioremediation and valorization of cattle wastewater previously digested in a hybrid anaerobic reactor using a photobioreactor: comparison between batch and … HV de Mendonça, JPHB Ometto, MH Otenio, IPR Marques, AJD Dos Reis Science of the Total Environment 633, 1-11 , 2018 2018 Citations: 91
Dynamics of antibiotic resistance genes and presence of putative pathogens during ambient temperature anaerobic digestion JA Resende, CG Diniz, VL Silva, MH Otenio, A Bonnafous, PB Arcuri, ... Journal of applied microbiology 117 (6), 1689-1699 , 2014 2014 Citations: 72
Biofuel recovery from microalgae biomass grown in dairy wastewater treated with activated sludge: The next step in sustainable production HV de Mendonca, MH Otenio, L Marchao, A Lomeu, DS de Souza, A Reis Science of The Total Environment 824, 153838 , 2022 2022 Citations: 62
Noise level in a 222 bed hospital in the 18th health region-PR MH Otenio, E Cremer, EMT Claro Revista brasileira de otorrinolaringologia 73, 245-250 , 2007 2007 Citations: 60
Intensidade de ruído em hospital de 222 leitos na 18ª Regional de Saúde-PR MH Otenio, E Cremer, EMT Claro Revista brasileira de otorrinolaringologia 73 (2), 245-250 , 2007 2007 Citations: 54
Quantitative microbial risk assessment of occupational and public risks associated with bioaerosols generated during the application of dairy cattle wastewater as biofertilizer A de Matos Nascimento, VR de Paula, EHO Dias, J da Costa Carneiro, ... Science of the Total Environment 745, 140711 , 2020 2020 Citations: 53
Ocorrência de Pseudomonas aeruginosa em água potável NMM Guerra, MH Otenio, MEZ Silva, M Guilhermetti, CV Nakamura, ... Acta Scientiarum. Biological Sciences 28 (1), 13-18 , 2006 2006 Citations: 52
Seasonal variation on microbial community and methane production during anaerobic digestion of cattle manure in Brazil JA Resende, JJ Godon, A Bonnafous, PB Arcuri, VL Silva, MH Otenio, ... Microbial ecology 71 (3), 735-746 , 2016 2016 Citations: 42
Biofertilizer Application on Corn ( Zea mays ) Increases the Productivity and Quality of the Crop Without Causing Environmental Damage A de Matos Nascimento, AM Maciel, JBG Silva, HV Mendonça, ... Water, Air, & Soil Pollution 231 (8), 414 , 2020 2020 Citations: 41
Production of energy and biofertilizer from cattle wastewater in farms with intensive cattle breeding HV de Mendonça, JPHB Ometto, MH Otenio Water, Air, & Soil Pollution 228 (2), 72 , 2017 2017 Citations: 39
Optimization of the skimmed-milk flocculation method for recovery of adenovirus from sludge ASF Assis, MH Otenio, BP Drumond, TM Fumian, MP Miagostovich, ... Science of the Total Environment 583, 163-168 , 2017 2017 Citations: 32
Manual de bovinocultura de leite AM AUAD, AMB SANTOS, AV CARNEIRO, A RIBEIRO, AC CARVALHO, ... Brasília: LK editora , 2010 2010 Citations: 32
Optimization of ozone application in post-treatment of cattle wastewater from organic farms DS de Souza, AM Maciel, MH Otenio, HV de Mendonça Water, Air, & Soil Pollution 231 (7), 362 , 2020 2020 Citations: 30
Bioenergy recovery from cattle wastewater in an UASB-AF hybrid reactor HV de Mendonça, JPHB Ometto, MH Otenio, AJD Dos Reis, IPR Marques Water Science and Technology 76 (9), 2268-2279 , 2017 2017 Citations: 27
Aplicação de biofertilizante de bovinocultura leiteira em um planossolo AM Maciel, JBG Silva, A de Matos Nascimento, VR de Paula, MH Otenio Revista em Agronegócio e Meio Ambiente 12 (1), 151-171 , 2019 2019 Citations: 25
Relationship between viral detection and turbidity in a watershed contaminated with group A rotavirus ASF Assis, LT Cruz, AS Ferreira, ME Bessa, MA de Oliveira Pinto, ... Environmental Science and Pollution Research 22 (9), 6886-6897 , 2015 2015 Citations: 22
Potential for nutrients reuse, carbon sequestration, and CO2 emissions reduction in the practice of domestic and industrial wastewater recycling into agricultural soils: A review TG Carpanez, JBG Silva, MH Otenio, MCS Amaral, VR Moreira Journal of Environmental Management 370, 122443 , 2024 2024 Citations: 19
Biofertilizer replace urea as a source of nitrogen for sugarcane production HV de Mendonça, CE Martins, WSD da Rocha, CAV Borges, ... Water, Air, & Soil Pollution 229 (7), 216 , 2018 2018 Citations: 17
