Ali Hassan Daghir Janabi

@vm.qu.edu.iq

Department of Microbiology
College of Veterinary Medicine, University of Al-Qadisiyah

Ali Hassan Daghir Janabi


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https://researchid.co/ahdj2024

EDUCATION

PhD in Microbial Biology, Rutgers University, USA

RESEARCH, TEACHING, or OTHER INTERESTS

Microbiology
10

Scopus Publications

140

Scholar Citations

6

Scholar h-index

6

Scholar i10-index

Scopus Publications

  • Complete genome sequence and functional characterization of Bacillus amyloliquefaciens NJF-55: a sheep-derived probiotic candidate
    Baraa Akeel Al-Hasan, Ali H. D. Janabi, Carina Helmer
    Scientific Reports, 2026
    Bacillus amyloliquefaciens strain NJF-55, which was isolated from the distal colon of a healthy ram, was investigated as a probiotic candidate. We produced a high-quality assembly comprising a single circular chromosome (3,854,703 bp; 46.1% GC) and a small plasmid (6033 bp), and reconciled multi pipeline annotations to derive a consensus feature set. Functional profiling mapped 2334 genes to 2027 KEGG orthologs (1835 unique), indicating complete central metabolism, broad transport capacity, and stress-adaptation pathways; 94 CAZymes supported glycan turnover and cell-envelope remodeling. Secondary-metabolite mining revealed hallmark Bacillus BGCs—surfactin, fengycin, bacillaene, and macrolactin—and flagged putative novel compounds (a PKS-like region with ~ 7% similarity to the butirosin A/B reference); Five bacteriocin/RiPP regions were identified (class IV lanthipeptide, amylocyclicin, ComX2 pheromone, UviB-like and colicin-like). Genome-safety screening detected no classical virulence determinants or acquired clinically relevant AMR genes; four intact prophages lacked toxin/AMR cargo, and the plasmid encoded no risk loci. Comparative genomics with four reference bacilli yielded a 5,398-cluster pangenome; NJF-55 retained 325 probiotic-associated clusters (largely core) and showed 30 copy-number expansions, notably in the NRPS/PKS and DNA replication/repair families, consistent with a “core-driven, dosage-tuned” architecture. These features suggest that NJF-55 may represent a safe and robust candidate for ruminant gut applications, while underscoring the need for targeted metabolomics and controlled in vivo validation.
  • Metabolomics-detected alterations generated by phytosomal propolis and phytosomal Lycopene in male rats with induced benign prostatic hyperplasia
    Ali M. Ghazi, Mohanad A. Ali Al-Bayati, Ali H. Janabi
    Iraqi Journal of Veterinary Sciences, 2024
    Benign prostatic hyperplasia (BPH) is a common chronic benign increase in the size of the prostate gland, in which the incidence rate elevates gradually with age. Despite the abundance of research regarding BPH, the treatment is still a significant challenge. The present study was conducted to estimate the blood metabolomics changes generated by the effects of phytosomal propolis (PP) and phytosomal Lycopene (PL) in male rats with induced BPH. For this purpose, BPH was induced in 25 adult male rats by the administration of testosterone propionate (T) and estradiol valerate (E2) at 200µg/100g and 40µg/100g, respectively, three subcutaneously (S/C) injections each per week for four weeks. Besides, 14 rats were only exposed to the solvent S/C, sterile corn oil (SCO), per the above criteria. The rats with no BPH were assigned into the following control groups; B: The negative control that received BPS (B) at 2ml/200g B.W. and Ls-1: The rat group that received liposome (Ls) at 2mg/Kg B.W. The males with BPH were divided into the following groups: 0: The positive control that received nothing (0), Ls-2: The treatment group that received Ls at 2mg/kg B.W., PP: The treatment group that received PP at 2mg/kg B.W., PL: The treatment group that received PL at 2mg/kg B.W., and PPPL: The treatment group that received a combination of PP and PL at 2mg/kg B.W. All treatments were applied orally by gavage once daily for four weeks. After the experiment, blood samples were collected from the hearts subjected to blood plasma separation. Then, plasma metabolites were extracted, which was followed by GC-Mass analysis. A wide range of changes were seen in treated groups at the level of different metabolites. For plasma components, such as L-alanine, Lauric acid, linoleic acid, L-lysine, L-proline, myristic acid, pyruvic acid, and succinic acid, significant (P<0.05) increases were recorded in the phytosomal treated groups. Alterations in the blood metabolites are recorded, and there are substantial increases in some fatty acids' levels, probably due to shifts in specific pathways belonging to eukaryotic or microbiota systems. These alterations may help to reduce the impact of BPH.
  • ANTI-OXIDANT AND ANTI-MICROBIAL EVALUATION OF LYCOPENE ISOLATED FROM WATERMELON
    Biochemical and Cellular Archives, 2021
  • Molecular docking analysis of anti-severe acute respiratory syndrome-Coronavirus 2 ligands against spike glycoprotein and the 3-chymotrypsin-like protease
    Journal of Medical Signals and Sensors, 2021
  • Effective anti-sars-cov-2 rna dependent rna polymerase drugs based on docking methods: The case of milbemycin, ivermectin, and baloxavir marboxil
    Avicenna Journal of Medical Biotechnology, 2020
  • The role of gut bacterial cytochrome-P450 of mosquito larvae in degradation of temephosas insecticide
    Mohammed Mahdi Yaseen, Azhar Chafat Karawan, Monyer Abdulameir Abd Alfatlawi, Ali H.D. Janabi
    Annals of Tropical Medicine and Public Health, 2020
    The present experimental work was generated to test the hypothesis that if there is a role of larval-related bacteria in protecting the host larvae from larvicides via bacterial-cytochrome-P450-based degradation. Here, a group of 50 larvae of Aedesalbopictus was subjected to temephos at 0.5 mg/land ketoconazole, an inhibitor of the bacterial cytochrome P450, at200mg/50 liter(TKG), 50 larvae received ketoconazole only at 200mg/50 liter (KG), and 50 larvae that played as a control group (CG) were only subjected to the larvicide. The grouping was made in a triplicate for each group.The larvae were monitored for livability every day until the end of the experiment that lasted for 2 days.The results indicated 88% and 100% mortalities in the TKG larvae in the first and the second day respectively of the experiment. However, partial deaths were seen in the CG larvae as 30 (60%) and 20 (100%) at day 1 and 2 respectively. Moreover, 10%of the larvae died in the KG when ketoconazole was used. TKG revealed significant (p<0.05) increases in the mortalities more than that in the CG and the KG. Day 2 of the experiment showed 100% mortalities in both TKG and CG larvae.This experiment provides valuable information that larvalJanabi et al (2020): Bacterial cytochrome-P450 of mosquito January 2020 Vol. 23 (I) ©Annals of Tropical Medicine & Public Health S412 related bacteria act as potential protectors against the killing activities of larvicides via the degradation activity of the bacterial cytochrome P450.
  • First phylogenetic characterization of pseudocowpox virus from cattle in Al-Qadisiyah province-Iraq
    Salah Karim, Khalefa Mansour, Ali Janabi, Nawras Al-Nakeeb
    Iraqi Journal of Veterinary Sciences, 2018
    This study was initiated for the first time for identification, using sequencing and phylogenetic analyses, of pseudocowpox PCPV that inhabit dairy cows in Al-Qadisiyah province, Iraq. Scab sampling was performed to obtain specimens from udder and teats of 18 affected cows. Initially, a polymerase chain reaction (PCR) method was followed to target a 408-bp piece of the GM_CSF/IL-2 inhibition factor gene (GIF) that belongs to PCPV. Then, the PCR products were sent out to partial sequencing of the GIF gene. The results of the PCR have indicated the presence of the virus in only 3 out of 18 samples. When the sequences were studied using phylogeny, the results have revealed that one of our PCPV strains has a close matching with some of the world strains such as from New Zealand. While two of the current study strains have clustered together with a strain from Finland. The results of our study confirm the presence of the PCPV in dairy cows that induces milker’s nodules.
  • The effects of acute strenuous exercise on the faecal microbiota in Standardbred racehorses
    A.H.D. Janabi, A.S. Biddle, D.J. Klein, K.H. McKeever
    Comparative Exercise Physiology, 2017
    While exercise has been found to change the faecal microbiome (FM) in laboratory animals exposed over weeks, no studies have identified immediate changes in the FM associated with short spans of intense exercise, ~5 min. The purpose of this study was to test the hypothesis that acute intense exercise would alter the FM in horses. Each horse performed two rounds of testing undergoing both a graded exercise test (GXT) and a parallel standing control (SC) trial before (GXT1 and SC1) and after (GXT2 and SC2) 12 weeks of exercise training. Rectal faecal samples were taken 24 h before and after testing. Bacterial community analysis was done by sequencing the 16s rRNA (V3-V4) region via Illumina Miseq. The relative abundance of the genus Clostridium significantly decreased in SC1 (P&lt;0.05), with a concurrent decrease in the Shannon diversity index at the species level (P&lt;0.05). At both the genus and species levels the principle coordinate analysis (PCoA) showed significant separation when the samples collected before SC1 were compared to those collected after SC1 (P&lt;0.05). Interestingly, we found that Fusicatenibacter saccharivorans, a bacteria found to be decreased in ulcerative colitis patients, and Treponema zioleckii, a bacteria found to degrade fructan in sheep rumen, were significantly decreased when the samples collected before SC1 were compared to those collected after SC1 (P&lt;0.05). None of the changes observed in SC1 happened in SC2 (P&gt;0.05). Our results indicate that very intense acute exercise does not alter the faecal microbiome of the Standardbred race horse and that 12 weeks of exercise training does not alter that response.
  • Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material
    Ali H.D. Janabi, Lee J. Kerkhof, Lora R. McGuinness, Amy S. Biddle, Kenneth H. McKeever
    Journal of Microbiological Methods, 2016
  • Exercise training-induced changes in the gut microbiota of Standardbred racehorses
    A.H.D. Janabi, A.S. Biddle, D. Klein, K.H. McKeever
    Comparative Exercise Physiology, 2016
    Exercise has a significant effect on different physiological systems in the body of human and animals. Only limited numbers of published studies in laboratory animals or humans have shown the effect of exercise on the gut microbiota, and no studies have shown this effect in horses. In this study, 8 horses (4 mares, 4 geldings) were exercise trained for 12 weeks, and 4 additional mares were used as a parallel seasonal control. To identify bacterial community changes over time for both groups, rectal faecal samples were collected, DNA was extracted, and the 16S rRNA gene (V3-V4) was sequenced using the Illumina Miseq platform. One-way ANOVA, Shannon diversity index, and Principal Coordinate Analysis (PCoA) were used to identify differences between and among samples. The exercise training group showed significant changes in the levels of Bacteroidetes, Proteobacteria, and Spirochaetes phyla (P&lt;0.05), while there were no changes in the gut microbiota of the seasonal control group through the three months of the study (P&gt;0.05). Moreover, with training two genera significantly changed in their relative abundance over time, namely Clostridium and Dysgonomonas (P&lt;0.05). Dysgonomonas spp. was significantly changed in abundance during the exercise training period (P&lt;0.05). Also Treponema spp. showed significant changes during the exercise training period (P&lt;0.05). Shannon diversity index was decreased (P&lt;0.05) in the exercise group at the beginning of the study, but then returned to pre-training levels. PCoA showed significant separation between time points of the exercise training group as far as the levels of genera and species (P&lt;0.05) represented. Our results show that exercise training influences the gut microbiota, especially at the beginning of training.

RECENT SCHOLAR PUBLICATIONS

  • Chromosome-scale scaffold genome sequence of Arthrobacter citreus strain NJF-O2 isolated from the distal colon of a healthy sheep
    AS Wadday, BA Al-Hasan, AHD Janabi
    Microbiology Resource Announcements, e00200-26 , 2026
    2026
  • Complete genome sequence and functional characterization of Bacillus amyloliquefaciens NJF-55: a sheep-derived probiotic candidate
    BA Al-Hasan, AHD Janabi, C Helmer
    Scientific Reports , 2026
    2026
  • Molecular docking analysis of anti-severe acute respiratory syndrome-coronavirus 2 ligands against spike glycoprotein and the 3-chymotrypsin-like protease
    AHD Janabi
    Journal of Medical Signals & Sensors 11 (1), 31-36 , 2021
    2021
    Citations: 11
  • Effective anti-SARS-CoV-2 RNA dependent RNA polymerase drugs based on docking methods: the case of milbemycin, ivermectin, and baloxavir marboxil
    AHD Janabi
    Avicenna Journal of Medical Biotechnology 12 (4), 246 , 2020
    2020
    Citations: 16
  • First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province-Iraq.
    SM Karim, KA Mansour, AHD Janabi, NKM Al-Nakeeb
    Iraqi Journal of Veterinary Sciences 33 (1) , 2019
    2019
    Citations: 13
  • Metronidazole as a feed additive targeting archaea to potentially reduce frothy bloat in Iraqi local cows.
    SA Sabeeh, TM Esraa, FA Alelaah Ali, RJT Al-Baghdadi, AHD Janabi
    Biochemical & Cellular Archives 18 (2) , 2018
    2018
    Citations: 6
  • The effects of acute strenuous exercise on the faecal microbiota in Standardbred racehorses
    AHD Janabi, AS Biddle, DJ Klein, KH McKeever
    Comparative Exercise Physiology 13 (1), 13-24 , 2017
    2017
    Citations: 20
  • Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material
    AHD Janabi, LJ Kerkhof, LR McGuinness, AS Biddle, KH McKeever
    Journal of microbiological methods 129, 14-19 , 2016
    2016
    Citations: 42
  • Exercise training-induced changes in the gut microbiota of Standardbred racehorses
    AHD Janabi, AS Biddle, D Klein, KH McKeever
    Comparative Exercise Physiology 12 (3), 119-130 , 2016
    2016
    Citations: 32

MOST CITED SCHOLAR PUBLICATIONS

  • Comparison of a modified phenol/chloroform and commercial-kit methods for extracting DNA from horse fecal material
    AHD Janabi, LJ Kerkhof, LR McGuinness, AS Biddle, KH McKeever
    Journal of microbiological methods 129, 14-19 , 2016
    2016
    Citations: 42
  • Exercise training-induced changes in the gut microbiota of Standardbred racehorses
    AHD Janabi, AS Biddle, D Klein, KH McKeever
    Comparative Exercise Physiology 12 (3), 119-130 , 2016
    2016
    Citations: 32
  • The effects of acute strenuous exercise on the faecal microbiota in Standardbred racehorses
    AHD Janabi, AS Biddle, DJ Klein, KH McKeever
    Comparative Exercise Physiology 13 (1), 13-24 , 2017
    2017
    Citations: 20
  • Effective anti-SARS-CoV-2 RNA dependent RNA polymerase drugs based on docking methods: the case of milbemycin, ivermectin, and baloxavir marboxil
    AHD Janabi
    Avicenna Journal of Medical Biotechnology 12 (4), 246 , 2020
    2020
    Citations: 16
  • First phylogenetic characterization of Pseudocowpox virus from cattle in Al-Qadisiyah province-Iraq.
    SM Karim, KA Mansour, AHD Janabi, NKM Al-Nakeeb
    Iraqi Journal of Veterinary Sciences 33 (1) , 2019
    2019
    Citations: 13
  • Molecular docking analysis of anti-severe acute respiratory syndrome-coronavirus 2 ligands against spike glycoprotein and the 3-chymotrypsin-like protease
    AHD Janabi
    Journal of Medical Signals & Sensors 11 (1), 31-36 , 2021
    2021
    Citations: 11
  • Metronidazole as a feed additive targeting archaea to potentially reduce frothy bloat in Iraqi local cows.
    SA Sabeeh, TM Esraa, FA Alelaah Ali, RJT Al-Baghdadi, AHD Janabi
    Biochemical & Cellular Archives 18 (2) , 2018
    2018
    Citations: 6
  • Chromosome-scale scaffold genome sequence of Arthrobacter citreus strain NJF-O2 isolated from the distal colon of a healthy sheep
    AS Wadday, BA Al-Hasan, AHD Janabi
    Microbiology Resource Announcements, e00200-26 , 2026
    2026
  • Complete genome sequence and functional characterization of Bacillus amyloliquefaciens NJF-55: a sheep-derived probiotic candidate
    BA Al-Hasan, AHD Janabi, C Helmer
    Scientific Reports , 2026
    2026