Wearable Electrochemical Sensor Based on Laser-Induced Graphene on Adhesive Bandage Leyllanne K. A. Souza, Maxwell D. Bridges, Thaisa A. Baldo, Wendell K. T. Coltro, Charles S. Henry Ecs Sensors Plus, 2026 Wearable sensors enable continuous and non-invasive monitoring of biomarkers, facilitating home-based healthcare and reducing the reliance on costly laboratory tests. Here, we present a wearable electrochemical sensor based on laser-induced graphene (LIG) fabricated using polyimide and transferred onto a commercial adhesive bandage for the simultaneous detection of uric acid (UA) and ascorbic acid (AA) in sweat. The electrodes displayed a carbon-rich, homogeneous morphology with a hydrophilic surface, enhancing analyte interaction. The repeatability and reproducibility studies showed satisfactory relative standard deviations (RSD) values equal to 2.7 and 8.2%, respectively. Analytical performance for AA and UA was evaluated by differential pulse voltammetry (DPV) measurements in phosphate-buffered saline (PBS). In simultaneous detection, the sensor exhibited a linear response from 20 to 200 μmol l −1 (R 2 = 0.99) for AA and UA. The limit of detection (LOD) was 6.6 μmol l −1 and 6 μmol l −1 for AA and UA, respectively. The device was further applied in artificial sweat, exhibiting minimal interference (<10%) and recoveries of 96%–100% for AA and 90%–115% for UA (n = 3). These results demonstrate the potential of this low-cost (∼US$0.5/unit) flexible sensor as a practical platform for biofluid analysis.
Determination of Monoethylene Glycol in Gas Condensate Samples by Microchip Micellar Electrokinetic Chromatography Integrated With Capacitively Coupled Contactless Conductivity Detection Maurício M. L. Pereira, Kariolanda C. A. Rezende, Iris Medeiros Junior, Bruno Charles do Couto, Rogerio M. Carvalho, et al. Electrophoresis, 2026 This study describes the use of microchip micellar electrokinetic chromatography (MEKC) integrated with capacitively coupled contactless conductivity detection (C 4 D) for the determination of monoethylene glycol (MEG) in gas condensate samples. The samples were subjected to a liquid–liquid extraction step and then analyzed by chip‐based MEKC‐C 4 D. For this purpose, sodium dodecyl sulfate (SDS) was used as a surfactant at a concentration of 30 mmol L −1 added in 50 mmol L −1 phosphate (pH = 9.0). Samples were introduced into microchannels through floating injection mode by applying a voltage of 600 V during 10 s. Separations were performed under an electric field of 82 V cm −1 and monitored by C 4 D measurements recorded applying a 1200‐kHz frequency sinusoidal wave with 20‐V pp excitation voltage. The proposed methodology employing MEKC‐C 4 D revealed a linear behavior (r 2 ≥ 0.99) in the MEG concentration range between 150–450 µmol L −1 and LOD equal to 33 µmol L −1 . Three gas condensate samples were then analyzed, and the achieved MEG concentration values ranged from 173 to 213 µmol L −1 . Recovery experiments provided values between 89 and 102%. Based on the results reported in this study, MEKC‐C 4 D devices have demonstrated to be a promising and ecological analytical tool for MEG analysis with huge potential for in‐field applications.
On-site analytical techniques for forensic chemistry Thiago M.G. Cardoso, Kemilly M.P. Pinheiro, Diego P. Siqueira, Wendell K.T. Coltro Green Analytical Methods and Miniaturized Sample Preparation Techniques for Forensic Drug Analysis, 2024
Wearable hybrid sensors Pedro V.V. Romanholo, Habdias A. Silva-Neto, Lívia F. Sgobbi, Wendell K.T. Coltro Wearable Physical Chemical and Biological Sensors Fundamentals Materials and Applications, 2022
Sensing Materials: Paper Substrates Lucas R. Sousa, Habdias A. Silva-Neto, Nikaele S. Moreira, Bárbara G.S. Guinati, Wendell K.T. Coltro Encyclopedia of Sensors and Biosensors Volume 1 4 First Edition, 2022
Sensing Materials: Paper Substrates Lucas R. Sousa, Habdias A. Silva-Neto, Nikaele S. Moreira, Bárbara G.S. Guinati, Wendell K.T. Coltro Encyclopedia of Sensors and Biosensors First Edition Four Volume Set, 2022
Colorimetric paper-based analytical devices Habdias A. Silva-Neto, Lucas R. Sousa, Wendell K.T. Coltro Paper Based Analytical Devices for Chemical Analysis and Diagnostics, 2021
Paper-based separation devices Cyro L.S. Chagas, Nikaele S. Moreira, Bárbara G.S. Guinati, Wendell K.T. Coltro Paper Based Analytical Devices for Chemical Analysis and Diagnostics, 2021
19th Brazilian meeting on analytical chemistry Wendell K.T. Coltro, Fábio R.P. Rocha, Leonardo S.G. Teixeira, Fernando F. Sodré, Ricardo E. Santelli, et al. Microchemical Journal, 2020
Real-time monitoring of the microdroplet size on 3D printed microfluidic devices with integrated contactless conductivity detection 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2017, 2020
Fabrication of paper-based microfluidic devices with scholar glue and magnetic masks 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2017, 2020
Capillary and Microchip Electrophoresis Lucas Blanes, Ellen Flávia Moreira Gabriel, Renata Mayumi Saito, Wendell Karlos Tomazelli Coltro, Nerida Cole, et al. Analytical Techniques in Forensic Science, 2020
Paper-based microfluidic devices on the crime scene: A simple tool for rapid estimation of post-mortem interval using vitreous humour 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2017, 2020
Fast and versatile fabrication of PMMA microchip electrophoretic devices by laser engraving 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2014, 2014
Rational selection of substrates to improve color intensity and uniformity on microfluidic paper-based analytical devices 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2014, 2014
Stamping of microfluidic paper-based analytical devices with chemically modified surface for clinical diagnostics 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2014, 2014
Ionic electrodes for capacitively coupled contactless conductivity detection on electrophoresis microchips 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2014, 2014
Fabrication of disposable electrophoresis microchips based on using of colored toner Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012, 2012
Fabrication of capillary-driven toner-based microfluidic devices for clinical diagnostics with colorimetric detection Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2012, 2012
Real-time monitoring of binding assays on microfluidic devices with contactless conductivity detection 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences the Proceedings of Microtas 2008 Conference, 2008
Low-cost technologies for microfluidic applications Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques Third Edition, 2007
Electrochemical Strategies for Lateral Flow and LAMP-Based Platforms Toward Pathogen Detection: A critical review LF de Lima, PFN Estrela, L Lopes-Luz, LA Pradela-Filho, JV Souza, ... Analytica Chimica Acta, 345476 , 2026 2026
Paper-based Microfluidic Devices and Their Fabrication LR Sousa, LG Velasco, SG Vlachovsky, F Figueredo, E Cortón, ... The Handbook of Paper-Based Sensors and Devices: Volume 2: Sensors, 543-571 , 2026 2026
Blue-for-Positive: Colorimetric LAMP detection on paper-based microfluidic devices using a blue-shift indicator for instrument-free naked-eye readout SG Vlachovsky, LR Sousa, F Stolowicz, A Vojnov, WKT Coltro, E Cortón, ... Analytica Chimica Acta, 345279 , 2026 2026
Sustainable Stencil-Printed Unmodified Graphite–Rosin Electrode for Electrochemical Detection of Ketamine in Forensic Samples JP Campos, LMA Melo, DS Rocha, DJA dos Santos, MML Pereira, ... Electrochimica Acta, 148448 , 2026 2026
A novel paper/plastic-based hybrid strip test for rapid and multiplexed colorimetric detection of milk adulterants LR Sousa, BGS Guinati, KA Oliveira, CLS Chagas, WKT Coltro Food Chemistry, 147956 , 2026 2026
Presumptive semen detection using fructose as a forensic marker: Comparison of colorimetric solution assays and paper-based analytical devices ALA Nascimento, DS Anunciação, WKT Coltro, JCC Santos Microchemical Journal, 116891 , 2026 2026
Exploring the role of microfluidic paper-based analytical devices in salivary diagnostics-from the concept to clinical applications L Rodrigues de Sousa, LG Velasco, SG Vlachovsky, F Figueredo, ... Royal Society of Chemistry , 2026 2026
Exploring the role of microfluidic paper-based analytical devices in salivary diagnostics-from the concept to clinical applications LR Sousa, LG Velasco, SG Vlachovsky, F Figueredo, E Cortón, ... Analyst , 2026 2026
Visible-light-driven electrophilic chalcogen species: integration of sustainable synthesis, green metrics, and computational discovery of antioxidants FT Coelho, LVE Guimarães, CP Ebersol, LG Velasco, P Da Costa, ... Organic Chemistry Frontiers 13 (6), 1737-1746 , 2026 2026 Citations: 1
Determination of Monoethylene Glycol in Gas Condensate Samples by Microchip Micellar Electrokinetic Chromatography Integrated With Capacitively Coupled Contactless Conductivity … MML Pereira, KCA Rezende, IM Junior, BC Couto, RM Carvalho, ... Electrophoresis , 2026 2026
A portable platform with integrated colorimetric and electrochemical detection of nitrite and bisulfite in environmental and beverage samples GR Souza, SC Guerra, PM de Alencar, LMA Melo, DJA Dos Santos, ... Analytical Methods , 2026 2026
Wearable Electrochemical Sensor Based on Laser-Induced Graphene on Adhesive Bandage LKA Souza, M Bridges, TA Baldo, W Coltro, CS Henry ECS Sensors Plus , 2026 2026 Citations: 1
Automated manufacturing of paper-based analytical devices through pen-plotting technique for depositing hydrophobic barriers and chromogenic reagents LKA Souza, DS Rocha, PVV Romanholo, LF Sgobbi, WKT Coltro Sensors and Actuators B: Chemical, 139386 , 2026 2026 Citations: 2
Drone-and Paper-Based Analytical Devices: A Powerful Combination for the Colorimetric Detection of Tropospheric Ozone PPE Campos, HA Silva-Neto, LC Duarte, JFS Petruci, WKT Coltro Analytical Chemistry 97 (29), 15818-15825 , 2025 2025 Citations: 4
Monitoring of monoethylene glycol in gas condensate samples using a 3D printed batch injection analysis cell coupled with cupric oxide sensing electrode for amperometric detection KCA Rezende, IM Júnior, WKT Coltro Microchemical Journal 209, 112754 , 2025 2025 Citations: 3
Recent developments on aerial lab-on-a-drone platforms for remote environmental monitoring: A review HA Silva-Neto, D da Silva Sousa, LC Duarte, JF da Silveira Petruci, ... Analytica Chimica Acta, 343544 , 2025 2025 Citations: 21
Screen-printing vs additive manufacturing approaches: recent aspects and trends involving the fabrication of electrochemical sensors LO Orzari, C Kalinke, HA Silva-Neto, DS Rocha, JR Camargo, WKT Coltro, ... Analytical chemistry 97 (3), 1482-1494 , 2025 2025 Citations: 73
rsc. li/analyst WKT Coltro 2025
Tailored ionic liquids for microfluidic paper-based analytical devices: boosting colorimetric performance for enzymatic glucose assays via cellulose surface modification DS Paula, LG Velasco, JCP Sousa, TMG Cardoso, MI Qadir, BG Vaz, ... Chemical Communications 61 (91), 17910-17913 , 2025 2025 Citations: 1
A paper-based analytical device for rapid colorimetric detection of aluminium in aerosol antiperspirants LL Ferreira, AA de Carvalho, MLC de Souza, ÍA Costa, GF Duarte-Junior, ... Analytical Methods 17 (19), 3976-3984 , 2025 2025 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Handbook of capillary and microchip electrophoresis and associated microtechniques JP Landers CRC press , 2007 2007 Citations: 404
Portable analytical platforms for forensic chemistry: a review WR de Araujo, TMG Cardoso, RG da Rocha, MHP Santana, RAA Munoz, ... Analytica chimica acta 1034, 1-21 , 2018 2018 Citations: 311
Rational selection of substrates to improve color intensity and uniformity on microfluidic paper-based analytical devices E Evans, EFM Gabriel, WKT Coltro, CD Garcia Analyst 139 (9), 2127-2132 , 2014 2014 Citations: 225
Highly sensitive colorimetric detection of glucose and uric acid in biological fluids using chitosan-modified paper microfluidic devices EFM Gabriel, PT Garcia, TMG Cardoso, FM Lopes, FT Martins, ... Analyst 141 (15), 4749-4756 , 2016 2016 Citations: 218
Salivary diagnostics on paper microfluidic devices and their use as wearable sensors for glucose monitoring LF de Castro, SV de Freitas, LC Duarte, JAC de Souza, TRLC Paixão, ... Analytical and bioanalytical chemistry 411 (19), 4919-4928 , 2019 2019 Citations: 199
Modification of microfluidic paper-based devices with silica nanoparticles E Evans, EFM Gabriel, TE Benavidez, WKT Coltro, CD Garcia Analyst 139 (21), 5560-5567 , 2014 2014 Citations: 196
Capacitively coupled contactless conductivity detection on microfluidic systems—ten years of development WKT Coltro, RS Lima, TP Segato, E Carrilho, DP de Jesus, CL do Lago, ... Analytical Methods 4 (1), 25-33 , 2012 2012 Citations: 195
Toner and paper‐based fabrication techniques for microfluidic applications WKT Coltro, DP de Jesus, JAF da Silva, CL do Lago, E Carrilho Electrophoresis 31 (15), 2487-2498 , 2010 2010 Citations: 188
Wearable electrochemical sensors for forensic and clinical applications PC Ferreira, VN Ataide, CLS Chagas, L Angnes, WKT Coltro, ... TrAC Trends in Analytical Chemistry 119, 115622 , 2019 2019 Citations: 186
Recent advances in low‐cost microfluidic platforms for diagnostic applications WK Tomazelli Coltro, CM Cheng, E Carrilho, DP de Jesus Electrophoresis 35 (16), 2309-2324 , 2014 2014 Citations: 184
A handheld stamping process to fabricate microfluidic paper-based analytical devices with chemically modified surface for clinical assays P de Tarso Garcia, TMG Cardoso, CD Garcia, E Carrilho, WKT Coltro Rsc Advances 4 (71), 37637-37644 , 2014 2014 Citations: 160
Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms TMG Cardoso, PT Garcia, WKT Coltro Analytical Methods 7 (17), 7311-7317 , 2015 2015 Citations: 151
Characteristic levels of some heavy metals from Brazilian canned sardines (Sardinella brasiliensis) CRT Tarley, WKT Coltro, M Matsushita, NE de Souza Journal of food composition and analysis 14 (6), 611-617 , 2001 2001 Citations: 133
Recent advances on paper-based microfluidic devices for bioanalysis HA Silva-Neto, IVS Arantes, AL Ferreira, GHM do Nascimento, GN Meloni, ... TrAC Trends in Analytical Chemistry, 116893 , 2023 2023 Citations: 129
3D printing of microfluidic devices with embedded sensing electrodes for generating and measuring the size of microdroplets based on contactless conductivity detection LC Duarte, CLS Chagas, LEB Ribeiro, WKT Coltro Sensors and Actuators B: Chemical 251, 427-432 , 2017 2017 Citations: 120
A handheld stamping process to fabricate microfluidic paper-based analytical devices with chemically modified surface for clinical assays PT Garcia, TMG Cardoso, CD Garcia, E Carrilho, WKT Coltro Rsc Advances 4 (71), 37637-37644 , 2014 2014 Citations: 114
Enhanced Analytical Performance of Paper Microfluidic Devices by Using Fe3O4 Nanoparticles, MWCNT and Graphene Oxide F Figueredo, PT Garcia, E Cortón, WKT Coltro ACS Applied Materials & Interfaces , 2016 2016 Citations: 108
Wearable and Biodegradable Sensors for Clinical and Environmental Applications TA Baldo, LF de Lima, LF Mendes, WR de Araujo, TRLC Paixão, ... ACS Applied Electronic Materials , 2021 2021 Citations: 95
Comparison of the analytical performance of electrophoresis microchannels fabricated in PDMS, glass, and polyester‐toner WKT Coltro, SM Lunte, E Carrilho Electrophoresis 29 (24), 4928-4937 , 2008 2008 Citations: 91
