Evaluation of Green-synthesized Zinc Oxide Nanoparticles for Antibacterial, Antioxidant, and Anticancer Properties with Application in Sunscreen Formulation Riddhee De, Gayatri Katkar, Shraddha Jaiswal, Vidya Sunil Tale Biomedical and Biotechnology Research Journal, 2026 Background: Zinc oxide nanoparticles (ZnONps) are safe, affordable, and versatile, with strong ultraviolet (UV) absorption and visible light transparency, making them excellent sunscreen agents. Plant extract-mediated synthesis of ZnONps provides an eco-friendly substitute for conventional chemical approaches by avoiding toxic reagents and provides bioactive properties. The study aims to develop non-comedogenic cream formulation using green synthesized ZnONps using the extract of Senna alata and Zingiber officinale - Allium sativum together which possess antibacterial, UV absorption, antioxidant, and anticancer properties. Methods: The characterization was performed using UV-visible spectroscopy, Field Emission Scanning Electron Microscopy, X-ray diffraction, and Fourier Transform Infrared (FTIR) spectroscopy. Antibacterial activity evaluated against Escherichia coli and Staphylococcus aureus . The antioxidant activity was determined by the 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay. The anticancer properties were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay using HeLa cells. The Sun Protection Factor (SPF) of the cream formulated by the incorporation of ZnONps was determined. Results: The crystalline sizes of ZnONps synthesized using S. alata leaf and a mixture of Z. officinale-A. sativum extract were 16.98 nm and 92.30 nm, respectively. The FTIR and DPPH assay results showed the presence of phytochemical residue. The antibacterial activities of synthesized ZnONps from the combination of A. sativum and Z. officinale extract have displayed better inhibition ( S. aureus : 14.67 ± 0.35 mm) against gram-positive bacteria and gram-negative bacteria ( E. coli : 22.63 ± 0.62 mm) than those made with S. alata extract ( E. coli 14 ± 0.32 mm and S. aureus 19.4 ± 0.17 mm). The formulated cream showed skin-friendly pH with SPF more than 15. Conclusion: The biosynthesized ZnONPs exhibited antimicrobial, anticancer, and antioxidant potential and can be exploited in bioactive cosmetic formulations.
Microbiota as Immunity Boosting Agent Vijay L. Ghori, Vaibhav Dagaji Aher, Purti Tripathi, Nivas V Arun, Vidya S. Tale, Naresh Podila, R. S. Raghuwanshi, S. Parthasarathy Nutraceuticals and Gut Health, 2026 The host immune system’s induction, training, and function are all profoundly impacted by the microbiota. To keep the host’s symbiotic 52relationship with these various and ever-changing bacteria going strong, the immune system has mostly evolved. When this partnership between the immune system and microbiota is working well, it can instigate protective responses to infections and keep regulatory mechanisms that help keep tolerance to harmless antigens maintained. In both healthy and unhealthy states, the commensal microbiota interacts in complex ways with the immune system of mammals. While the immune system coordinates the upkeep of essential aspects of the host–microbe symbiosis, the microbiome is vital in the training and development of the host’s innate and adaptive immune system. The pathogenesis of numerous immune-mediated illnesses is thought to be aided by imbalances in microbiota–immunity interactions in genetically susceptible hosts within specific environmental circumstances. A microbiota lacking in diversity and resilience to build balanced immune responses has evolved in high-income nations as a result of antibiotic abuse, dietary changes, and the loss of essential partners like worms. In regions where our interdependent interaction with microbiota has been most disrupted, these phenomena may help explain why autoimmune and inflammatory diseases have recently been on the rise. In this chapter, we give examples of molecular processes in the intestine and other organs that orchestrate the interactions between the microbiome and the immune system, as well as a general overview of these interactions and their functions in health and illness. We review what is known about the host immune–microbiome interactions, the obstacles to understanding these interactions causally, and the effects of these interactions on immunemediated diseases. We then talk about how this knowledge could lead to the creation of microbiome targeted therapeutic interventions in the future.
Temporal Profiling of Extracellular Polymeric Substances Associated with Biofilm Formation in Bacteria Isolated from Wound Infection Prabhavati Shinde, Shraddha Jaiswal, Vidya Tale Journal of Pure and Applied Microbiology, 2025 Chronic wounds pose a substantial challenge to the global healthcare system. Despite the development of diverse treatments targeting various aspects of wound healing, the formidable obstacle of polymicrobial biofilm consortia persists. The biofilm matrix comprises EPS (Extracellular Polymeric Substances), mainly contains proteins, carbohydrates and eDNA (Extracellular DNA). It offers numerous advantages to microorganisms within the biofilm. The objective of this study was to evaluate the ability of bacteria isolated from wound infections to form biofilm using various methods and to analyze the composition of their EPS within the biofilm. Bacteria were isolated from wound swabs and identification was done by MALDI-TOF MS. Biofilm production was determined with the Congo red agar (CRA), crystal violet staining and microtiter plate methods. The genes involved in biofilm formation such as icaA and clfA, were detected through PCR analysis. EPS components were measured at different incubation times, with carbohydrates quantified by phenol-sulfuric acid method, proteins determined by the Lowry method, and eDNA analyzed through agarose gel electrophoresis. All bacteria showed the ability of biofilm formation and were distinguished as strong, moderate or weak biofilm makers. In the EPS, carbohydrate content increased after 72 hours of incubation, protein content was highest after 48 hours, and eDNA levels were highest after 72 hours of incubation. This study highlighted that biofilm-forming bacteria express various molecules in their EPS at different time intervals and hold promise for advancing our understanding of structural changes of biofilm and architectural integrity and functionality of matrix components.
Recent Nanotechnological Trends in the Management of Microbial Keratitis Shraddha Jaiswal, Prabhavati Shinde, Vidya Tale Journal of Ophthalmic and Vision Research, 2024 Microbial keratitis (MK) is a sight-threatening ocular disease that needs rapid diagnosis and treatment to prevent more serious outcomes. The broad-spectrum topical antimicrobial treatment is currently the main pharmacological approach for MK management, yet its efficacy is increasingly challenged by evolving antimicrobial resistance, including multidrug resistance. Also, the ocular surface presents numerous challenges for standard topical drug delivery. The failure and ineffectiveness of current therapies have necessitated the development of novel therapeutic strategies to manage MK. With advances in nanotechnology in the biomedical field, various nanomaterials can be employed to control MK. The primary determinants of nanoparticles’ vast range of applications are their size, surface properties, and chemical makeup, which also happen to be the same elements that give rise to their poisonous and dangerous effects. In this study, we provide a perspective on the contact lens-associated corneal illnesses such as MK and explore how nanotechnology might help address this significant clinical issue. In addition, safety and toxicological concerns about the increasingly widespread use of contact lenses are also discussed.
Molecular Docking and Pharmacodynamic Study of Potential Inhibitors of Streptococcus mutans Biofilm Aditya Naman Soni, Meghna Varshney, Vidya Tale Biomedical and Biotechnology Research Journal, 2023 Background: Biofilm formation permits the accumulation and adhesion of cariogenic bacteria on the surface of the tooth, resulting in an increase in antibiotic resistance. Streptococcus mutans is most commonly linked to dental caries. Biofilm formation is mediated by glucan-binding protein C (GbpC). This study focuses on the identification and pharmacodynamic study of putative ligands from ginger extract for the inhibition of GbpC. Methods: Studies on docking were conducted using AutoDock. In addition, pharmacodynamic experiments were carried out using the online tools Swiss-ADME, Molinspiration, and PROTOX for physiochemical, bioactivity score, and toxicity prediction, respectively. Results: Binding affinity between sissostrin and the methylbutanoate-derived ligand was found to be − 9.1 kcal/mol. Both ligands were analyzed for their inhibitory potency, which was calculated to be 6.67. The ligand derived from methylbutanoate is eliminated because it violated Swiss-ADME. Sissostrin’s immunotoxicity is predicted by PROTOX II with a 0.66 probability. As a biofilm inhibitor, sissostrin with the optimal docking pose and the lowest toxicity can be chosen. For a comprehensive evaluation of the drug, future in vivo trials of sissostrin can be anticipated. Conclusion: The present work was carried out to computationally identify the putative ligands from ginger extract which bind to GbpC. GbpC is responsible for the formation of biofilm causing dental caries. Docking scores suggested that all four chosen ligands are potential GpbC inhibitors. A significant bioactivity score was predicted from Molinspiration software for sissostrin. This score indicates that sissostrin is the best ligand to function as a potential drug.
Evaluation of the antibacterial potential of traditional medicinal plants against bacteria isolated from dental caries Sonali Ambulkar, Vidya Tale, Shobha Khilari Journal of Pure and Applied Microbiology, 2021 Dental caries are one of the leading infectious microbial diseases globally. Streptococcus species are the predominant causative agents for the formation of dental caries. Various antibiotics have been reported for the treatment of dental caries in humans. However, owing to the increasing evidence of microbial resistance, there is a need to develop safe and effective alternative treatments for infections. Traditional medicinal plants and their bioactive products have been explored worldwide for the treatment of various diseases and infections. These plants have great potential for creating novel medications without any side effects. The present study aimed to elucidate the antibacterial potential of medicinal plants against biofilm-forming bacteria from dental caries. Bacteria from dental caries were isolated and identified using 16S rRNA sequencing technique and the predominant bacterial isolates were Streptococcus mutans (MH889143), Enterococcus faecalis (MH793461), Rothia dentocariosa (MH824681), and Streptococcus anginosus (MH889145). The antibacterial potential of seventeen medicinal plants was determined against these bacterial isolates using the agar well diffusion method. The aqueous extracts of Moringa oleifera, Ficus benghalensis, Ficus racemosa, Ficus religiosa, Senegalia catechu, Pistacia integerrima, and Quercus infectoria showed significant inhibition against all bacterial isolates. Pistacia integerrima and Quercus infectoria showed the maximum inhibition. The present study confirmed that traditional medicinal plants could be helpful for the treatment of oral and dental ailments.
Probiotics as potential producer of phytase Current Topics in Peptide and Protein Research, 2020
GREEN SYNTHESIS OF SILVER NANOPARTICLES: ITS EFFECT ON QUORUM SENSING INHIBITION OF URINARY TRACT INFECTION PATHOGENS Shaheen Sheikh, Vidya Tale Asian Journal of Pharmaceutical and Clinical Research, 2017 Objective: The present study was conducted to evaluate the antimicrobial and anti-quorum sensing (QS) ability of nanoparticles synthesized usingfruit wastes.Methods: QS is a system of response and stimuli correlated to population density. Bacteria by QS secrete certain signaling molecules called autoinducers. These bacteria also have a receptor that can specifically detect the signaling molecule known as an inducer. Since QS governs numerous processes in bacteria including virulence, QS inhibition promises to be an ideal target for the development of novel therapeutics. In the present study, silver nanoparticles (AgNPs) synthesized from the ginger, lemon peel, cinnamon, corn silk, pomegranate peel, and orange peel exhibited anti-QS properties by inhibiting violacein production in Chromobacterium violaceum assayed using agar well diffusion method.Results: AgNPs were synthesized from various cost-effective fruit waste sources. These AgNPs exhibited significant antibacterial and anti-QS properties.Conclusion: Hence, such sources can be explored for developing the effective therapy for urinary tract infections.Keywords: Silver nanoparticles, Quorum sensing, Urinary Tract infection.
Isolation, characterization and optimization of alkaline protease producing novel marine fungi Cladosporium cladosporioides Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2017
Isolation, characterization and antibiotic resistance pattern of nosocomial bacterial isolates Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2016
Evaluation of Green-synthesized Zinc Oxide Nanoparticles for Antibacterial, Antioxidant, and Anticancer Properties with Application in Sunscreen Formulation VST Riddhee De, Gayatri Katkar, Shraddha Jaiswal Biomedical and Biotechnology Research Journal (BBRJ) 10, 45-53 , 2026 2026
Biofilm Formation and Antibiotic Susceptibility Patterns in Contact Lens-Associated Bacteria SR Jaiswal, PS Shinde, VS Tale Korean Journal of Ophthalmology: KJO , 2026 2026
Temporal Profiling of Extracellular Polymeric Substances Associated with Biofilm Formation in Bacteria Isolated from Wound Infection VT Prabhavati Shinde, Shraddha Jaiswal Journal of Pure and Applied Microbiology , 2025 2025
Recent Nanotechnological Trends in the Management of Microbial Keratitis VT Shraddha Jaiswal, Prabhavati Shinde Journal of Ophthalmic and Vision Research 19 (4), 476-487 , 2024 2024 Citations: 1
COMPUTATIONAL ANALYSIS OF BIOMOLECULES OF MURRAYA KOENIGII AS A POTENT ANTICANCER AGENT VSTMAC Moholkar V. ,Premalatha S , Vijay D. Bagh , Sudhahar Dharmalingam ... African Journal of Biological sciences 6 (5), 684-695 , 2024 2024
A comprehensive review on keto diet on management of type 2 diabetes and obesity D Chandgude, P Tambat, V Tale Journal of Chemical Health Risks 13 (4), 609-614 , 2024 2024 Citations: 2
PCOS and its Preventive Measures VST Kshitija Tewary , Pranjalee S. Tambat Journal of Advanced Zoology 44 (7), 1259-1263 , 2023 2023
Molecular Docking and Pharmacodynamic Study of Potential Inhibitors of Streptococcus mutans Biofilm VST Aditya Naman Soni, Meghna Varshney Biomedical and Biotechnology Research Journal 7 (3), 471-7 , 2023 2023 Citations: 10
Cloning of laccase gene from Coriolopsis caperata into heterologous E.coli host M Wadgaonkar, V Tale, Y Chavan Vegetos 36 (1), 195-200 , 2023 2023 Citations: 2
Metal Nanoparticle Synthesis Using Fruit Extracts as Reducing Agents and Comparative Studies with a Chemical Reducing Agent JPVT Soham Lavande1, Shraddha Jaiswal1, Roshanee Deore1 Biosciences Biotechnology Research Asia 19 (2), 487-496 , 2022 2022 Citations: 2
Evaluation of the Antibacterial Potential of Traditional Medicinal Plants against Bacteria Isolated from Dental Caries SK Sonali Ambulkar, Vidya Tale J Pure Appl Microbiol 15 (3), 1204-1210 , 2021 2021 Citations: 7
Media Optimization and Comparative Study of White-Rot Fungi Species for Their Capacity to Produce Laccase Enzyme M Wadgaonkar, V Tale, Y Chavan Annals of the Romanian Society for Cell Biology, 12487-12499 , 2021 2021
Metagenomic analysis of Dam Reservoirs in Pune city for bacterial fingerprints through BLAST and Kaiju tool PC Patil, DA Patel, VS Tale Biosciences Biotechnology Research Asia 17 (4), 839-852 , 2021 2021 Citations: 2
An analysis of non-cultivable bacteria using WEKA PC Patil, PS Panchal, S Madiwale, VS Tale Bioinformation 16 (8), 620 , 2020 2020 Citations: 5
Biofilm forming ability of bacteria isolated from dental caries: with reference to Streptococcus species S Ambulkar, V Tale, A Jadhav, K Kulkarni Future Dental Journal of Egypt 5 (1), Article 2 , 2020 2020 Citations: 5
Probiotics as potential producer of phytase M Rane, V Tale Current Topics in Peptide & Protein Research 21, 37 - 43 , 2020 2020
Hydrothermal assisted biological synthesis of silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against … N Jain, P Bhosale, V Tale, R Henry, J Pawar EurAsian Journal of BioSciences 13 (1), 27-33 , 2019 2019 Citations: 24
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF QUERCUS INFECTORIA GALLS ON ROTHIA DENTOCARIOSA ISOLATED FROM DENTAL CARIES PJ AMBULKAR S1, TALE V1*, KHILARI S2 Asian J Pharm Clin Res 12 (10), 159-162 , 2019 2019 Citations: 6
Synthesis of copper oxide nanoparticles by chemical precipitation method for the determination of antibacterial efficacy against Streptococcus sp. and Staphylococcus sp. S SHIRSAT, D PAWAR, N JAIN, J PAWAR, VS TALE, R HENRY Synthesis 12 (5) , 2019 2019 Citations: 11
Extraction of Metagenome from Potable Water to Analyze Microbial Fingerprints VT Preeti Chunarkar Patil Open Access Journal of Science 2 (3) , 2018 2018
MOST CITED SCHOLAR PUBLICATIONS
Developments in analytical methods for detection of pesticides in environmental samples R Bhadekar, S Pote, V Tale, B Nirichan American Journal of Analytical Chemistry 2, 1 , 2011 2011 Citations: 93
Overview of microbial therapeutic enzymes P Mane, V Tale International Journal of Current Microbiology and Applied Sciences 4 (4), 17-26 , 2015 2015 Citations: 70
Comparative studies on potential probiotic characteristics of Lactobacillus acidophilus strains. G Dixit, D Samarth, V Tale, R Bhadekar EurAsian Journal of BioSciences 7 , 2013 2013 Citations: 59
Hydrothermal assisted biological synthesis of silver nanoparticles by using honey and gomutra (Cow Urine) for qualitative determination of its antibacterial efficacy against … N Jain, P Bhosale, V Tale, R Henry, J Pawar EurAsian Journal of BioSciences 13 (1), 27-33 , 2019 2019 Citations: 24
Green synthesis of silver nanoparticles: its effect on quorum sensing inhibition of urinary tract infection pathogens S Sheikh, V Tale Asian Journal of Pharmaceutical and Clinical Research, 302-305 , 2017 2017 Citations: 17
Biofilm formation on contact lenses by bacterial pathogens N Jain, P Bhosale, V Tale J. Pharm. Res 10 (1), 50-53 , 2016 2016 Citations: 16
Isolation and Characterization of Biofilm Forming Bacteria from Oral Microflora KD Sonkusale, VS Tale Int.J.Curr.Microbiol.App.Sci, 118-127 , 2015 2015 Citations: 16
Synthesis of copper oxide nanoparticles by chemical precipitation method for the determination of antibacterial efficacy against Streptococcus sp. and Staphylococcus sp. S SHIRSAT, D PAWAR, N JAIN, J PAWAR, VS TALE, R HENRY Synthesis 12 (5) , 2019 2019 Citations: 11
Molecular Docking and Pharmacodynamic Study of Potential Inhibitors of Streptococcus mutans Biofilm VST Aditya Naman Soni, Meghna Varshney Biomedical and Biotechnology Research Journal 7 (3), 471-7 , 2023 2023 Citations: 10
Identification and biotechnological potential of psychrotrophic marine isolates S Pote, Y Chaudhary, S Upadhayay, V Tale, S Walujkar, Y Shouche, ... EurAsian Journal of BioSciences 8, 51-60 , 2014 2014 Citations: 9
Evaluation of the Antibacterial Potential of Traditional Medicinal Plants against Bacteria Isolated from Dental Caries SK Sonali Ambulkar, Vidya Tale J Pure Appl Microbiol 15 (3), 1204-1210 , 2021 2021 Citations: 7
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF QUERCUS INFECTORIA GALLS ON ROTHIA DENTOCARIOSA ISOLATED FROM DENTAL CARIES PJ AMBULKAR S1, TALE V1*, KHILARI S2 Asian J Pharm Clin Res 12 (10), 159-162 , 2019 2019 Citations: 6
Isolation and characterization of biofilm forming Streptococcus species from oral flora of cancer patients AT Jadhav, VS Tale Int.J.Curr.Microbiol.App.Sci, 38-47 , 2015 2015 Citations: 6
Exploiting quorum sensing to inhibit the bacterial pathogens R Belapurkar, VS Tale, R Madkaikar Int J Curr Microbiol App Sci 3, 453-458 , 2014 2014 Citations: 6
An analysis of non-cultivable bacteria using WEKA PC Patil, PS Panchal, S Madiwale, VS Tale Bioinformation 16 (8), 620 , 2020 2020 Citations: 5
Biofilm forming ability of bacteria isolated from dental caries: with reference to Streptococcus species S Ambulkar, V Tale, A Jadhav, K Kulkarni Future Dental Journal of Egypt 5 (1), Article 2 , 2020 2020 Citations: 5
Evaluation of effect of non steroidal anti-inflammatory drugs on growth of Probiotics S Amin, VS Tale, RK Bhadekar, SN Jaiswal International Journal of Pure and Applied Sciences and Technology 20 (1), 25 , 2014 2014 Citations: 4
A comprehensive review on keto diet on management of type 2 diabetes and obesity D Chandgude, P Tambat, V Tale Journal of Chemical Health Risks 13 (4), 609-614 , 2024 2024 Citations: 2
Cloning of laccase gene from Coriolopsis caperata into heterologous E.coli host M Wadgaonkar, V Tale, Y Chavan Vegetos 36 (1), 195-200 , 2023 2023 Citations: 2
Metal Nanoparticle Synthesis Using Fruit Extracts as Reducing Agents and Comparative Studies with a Chemical Reducing Agent JPVT Soham Lavande1, Shraddha Jaiswal1, Roshanee Deore1 Biosciences Biotechnology Research Asia 19 (2), 487-496 , 2022 2022 Citations: 2
