Muhammad Usman

@web.uaf.edu.pk

PhD Scholar at Department of Plant Pathology, Faculty of Agriculture, UAF
Department of Plant Pathology, University of Agriculture Faisalabad

Muhammad Usman


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https://researchid.co/usmanshamoon
I am currently working on Nanotechnology, Induced Systemic Resistance (ISR) and antioxidant defense system of diseased & healthy plant

EDUCATION

PhD (Agriculture Plant Pathology)

University of Agriculture Faisalabad

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Plant Science, Plant Science, Plant Science
25

Scopus Publications

411

Scholar Citations

12

Scholar h-index

15

Scholar i10-index

Scopus Publications

  • Biocontrol potential of entomopathogenic nematodes and entomopathogenic fungi against the soil-dwelling stages of spotted-wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae)
    Rambika Thapa, Muhammad Usman, Emile Cole, Rufus Isaacs, Jaime C. Piñero, David Shapiro-Ilan, Yoensuk Chung, Marisol Quintanilla
    Biological Control, 2026
    Spotted-wing drosophila (SWD), Drosophila suzukii , is an invasive pest of small fruits such as blueberries and raspberries, causing substantial yield losses. This study evaluated the biocontrol potential of entomopathogenic nematodes (EPNs) and entomopathogenic fungi (EPF) against the last instar larvae and pupae of D. suzukii . In the first experiment, three EPN species, Steinernema carpocapsae (ALL strain), S. feltiae (SN strain), and Heterorhabditis bacteriophora (SN strain), were applied at a rate of 50 infective juveniles (IJs) cm⁻ 2 . S. feltiae was the most effective, reducing adult D. suzukii emergence to 20 % from larvae and 40 % from pupae. A subsequent experiment tested nine EPN species, including S. carpocapsae , S. riobrave (355 strain), S. glaseri (VS strain), H. indica (HOM1 strain), H. georgiana , H. floridensis , and H. megidis (UK211 strain). Among these, S. feltiae , S. carpocapsae , and S. glaseri consistently reduced adult emergence to below 50 %, with S. feltiae achieving 20 % emergence at 100 IJs cm⁻ 2 . In a third experiment, three EPF, Beauveria bassiana , Metarhizium brunneum, and Cordyceps fumosorosae , were evaluated. B. bassiana proved most effective, reducing D. suzukii adult emergence by more than 50 % at 1 × 10 8 conidia mL⁻ 1 . In the final experiment, combinations of S. carpocapsae , S. feltiae , or S. glaseri with B. bassiana at both 50 and 100 IJs cm⁻ 2 further reduced emergence, with the most effective treatment resulting in only 8 % adult emergence. These findings demonstrate the strong potential of EPNs and EPF, individually and in combination, as biocontrol agents targeting the soil-dwelling stages of D. suzukii .
  • First report of Curvularia hawaiiensis causing leaf spot disease of grape in Pakistan
    Muhammad Usman Ali, Muhammad Wahab, Muhammad Atiq, Muhammad Usman, Owais Iqbal, Muhammad Hamza Furqan, Ahmad Nawaz, Hamza Tariq, Ghalib Ayaz Kachelo, Nasir Ahmed Rajput
    Journal of Plant Pathology, 2025
  • Investigating the biochemical variations in onion leaves due to purple blotch disease and its management through induced resistance
    Muhammad Asghar, Muhammad Atiq, Muhammad Usman Ali, Ghalib Ayaz Kachelo, Nasir Ahmad Khan, Khalid Naveed, Muhammad Usman, Ahmad Nawaz, Owais Iqbal, Nasir Ahmed Rajput
    Plos One, 2025
    Purple blotch (PB), caused by Alternaria porri (Ellis) ciferri, poses a significant threat to onion crop, resulting in major economic losses in both bulb and seed production globally. The incidence of this disease underscores the critical need for an effective management. In the present study, screening of onion genotypes for PB under field conditions revealed that the genotypes like Phulkara and Ceylon expressed resistant, response with 8.21 and 8.91% disease severity index (DSI) respectively, whereas Desi Black and Red Imposta were highly susceptible, with DSI of 67.38 - 79.41%. Furthermore, resistant and susceptible genotypes were also evaluated for biochemical variation analysis. Significant variations (p ≤ 0.05) in antioxidant enzymes were observed across reaction groups (inoculated and un-inoculated), types (resistant and susceptible), and onion varieties in response to A. porri infection. The analysis of variance showed significant changes in antioxidant enzymes level of onion leaves, including catalase (CAT), peroxidase (POD), and super dismutase (SOD). Results indicated that the concentration of SOD (55.37%), POD (41.87%) and CAT (37.92), respectively, in resistant plant leaves, whereas susceptible varieties showed SOD (43.71%), POD (28.3%) and CAT (26.59%). Furthermore, the amount of antioxidant enzymes was reduced in both resistant as well as susceptible varieties of onion after inoculation. Amount of SOD (68.04%), POD (57.45%) and CAT (50.87%) were recorded in un-inoculated group of onion plants that reduced to 31.05, 12.98 and 13.64% in inoculated group respectively. For the management of A. porri four different plant activators (salicylic acid, benzoic acid, citric acid and di-potassium hydrogen phosphate) at three different concentrations (0.5, 0.75 and 1%) were evaluated under greenhouse and field conditions. Among all salicylic acid was found to be most effective in controlling this disease under greenhouse and field conditions. The present study revealed that purple blotch affects the biochemical mechanism of the plants, which helps in activating the resistance process against pathogens through various enzymes. Additionally, salicylic acid demonstrated significant efficacy in controlling purple blotch in onions.
  • Computational and experimental approaches to explore defense related enzymes conferring resistance in Fusarium infected chilli plants by regulating plant metabolism through nutritional products
    Muhammad Usman, Muhammad Atiq, Nasir Ahmed Rajput, Muhammad Sagheer, Ye Xia
    Plos One, 2025
    Nutritional status being the first line of defense for host plants, determines their susceptibility or resistance against invading pathogens. In recent years, the applications of plant nutrient related products have been documented as one of the best performers and considered as alternatives or/and supplements in plant disease management compared to traditional chemicals. However, knowledge about application of plant nutrient related products for the management of destructive fungal pathogen Fusarium oxysporum f.sp. capsici and their impact on the components of the antioxidant defense system, especially in chilli plants, still needs to be discovered. Therefore, in this current study, we aimed to evaluate two nutrient fertilizers viz. Krystafeed and Micro Plus at three different concentrations by soil drenching method for their effects against the Fusarium wilt of chilli and investigate the components of the antioxidant defense system of chilli plants. Correlation and computational analysis on the components of antioxidant defense system in various pathways were performed to predict the suitable binding sites of mineral ions. Results indicated that the combination of Krystafeed and Micro Plus was found the most effective with (27.01, 26.59%) disease incidence, followed by Micro Plus (29.56, 32.35%) and Krystafeed (38.21, 41.15%), both in greenhouse and field conditions, respectively. Moreover, the combination of Krystafeed and Micro Plus significantly increased the concentration of SOD (27.53, 108.96)%, POD (37.29, 45.65)%, CAT (19.33, 95.33)%, H2O2 (22.13, 118.98)%, TPC (27.39, 17.37)%, chlorophyll a (21.80, 35.74)%, chlorophyll b (57.57, 18.25)%, total chlorophyll (30.21, 19.83)%, Tocopherol (13.08, 33.66)%, TrxR (5.03, 36.56)%, MDA (13.84, 54.79)%, ascorbate (4.72, 17.28)%, Proline (5.94, 59.31)%, and phytoalexin (Capsidiol) (11.33, 55.08)% in the treated plants of resistant and susceptible chilli varieties, respectively, as compared to the untreated plants. Pearson’s correlation heat-map analysis showed that all the enzymes of antioxidant defense system were found positively correlated with each other. It is concluded that the improvement of crop resistance by the application of plant nutrient related products may be viable alternatives to synthetic chemicals for managing Fusarium wilt disease of chilli and potentially other pathogens.
  • Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency
    Pu Yuan, Muhammad Usman, Wenshan Liu, Ashna Adhikari, Chunquan Zhang, Victor Njiti, Ye Xia
    Biotechnology Journal, 2024
    Plant gene editing technology has significantly advanced in recent years, thereby transforming both biotechnological research and agricultural practices. This review provides a comprehensive summary of recent advancements in this rapidly evolving field, showcasing significant discoveries from improved transformation efficiency to advanced construct design. The primary focus is on the maturation of the Clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas)9 system, which has emerged as a powerful tool for precise gene editing in plants. Through a detailed exploration, we elucidate the intricacies of integrating genetic modifications into plant genomes, shedding light on transport mechanisms, transformation techniques, and optimization strategies specific to CRISPR constructs. Furthermore, we explore the initiatives aimed at extending the frontiers of gene editing to nonmodel plant species, showcasing the growing scope of this technology. Overall, this comprehensive review highlights the significant impact of recent advancements in plant gene editing, illuminating its transformative potential in driving agricultural innovation and biotechnological progress.
  • Entomopathogenic Fungi as Alternatives to Chemical Acaricides: Challenges, Opportunities and Prospects for Sustainable Tick Control
    Mahvish Rajput, Muhammad Sohail Sajid, Nasir Ahmed Rajput, David Robert George, Muhammad Usman, Muhammad Zeeshan, Owais Iqbal, Bachal Bhutto, Muhammad Atiq, Hafiz Muhammad Rizwan, Ian Kirimi Daniel, Olivier Andre Sparagano
    Insects, 2024
    Entomopathogenic fungi (EPFs) can infect and kill a diverse range of arthropods, including ticks (Acari: Ixodidae) that can transmit various diseases to animals and humans. Consequently, the use of EPFs as a biocontrol method for managing tick populations has been explored as an alternative to chemical acaricides, which may have harmful effects on the environment and non-target species. This review summarizes studies conducted on EPFs for tick control between 1998 and 2024, identifying 9 different EPF species that have been used against 15 different species of ticks. One of the most well-known and widely researched EPFs used against ticks is Metarhizium anisopliae, a fungus known for its ability to infect and kill various arthropods. When applied to tick-infested areas, M. anisopliae spores attach to the tick’s cuticle, germinate, and penetrate through the cuticle, leading to the eventual death of the tick due to the fungal infection. Whilst a number of studies support the potential of this and other EPF species against ticks, this review suggests that limitations to their effective use may include factors such as heat, humidity, and ultraviolet light (UV-A and UV-B). This comprehensive review aims to provide an overview of the literature on the potential of EPFs in tick control, focusing on their mode of action, previous field successes/failures, advantages, potential applications, and prospects for future practical developments.
  • DETECTION OF PHYTOPHTHORA NICOTIANAE INDUCED CITRUS GUMMOSIS BY THE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
    Hadeed Ahmad, Nasir Ahmed Rajput, Muhammad Atiq, Ghalib Ayaz Kachelo, Muhammad Usman, Hamza Tariq, Muhammad Wahab
    Pakistan Journal of Botany, 2024
  • Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
    Mohsin Shad, Arshia Nazir, Muhammad Usman, Muhammad Waheed Akhtar, Muhammad Sajjad
    International Journal of Biological Macromolecules, 2024
  • Surveillance and management of brown spot of potato
    Muhammad Atiq, Muhammad Faizan Ullah, Nasir Ahmed Rajput, Akhtar Hameed, Salman Ahmad, Muuhammad Usman, Abuzar Hasnain, Ahmad Nawaz, Shahid Iqbal, Hadeed Ahmad
    Archives of Phytopathology and Plant Protection, 2024
    A survey was conducted to assess the incidence of brown spot disease in three tehsils of District Okara. Maximum incidence was observed in Depalpur (44%) followed by Okara (33%) and Renala khurd (15%). During study, six chemicals (CabrioTop, Fossil, Axel, Forum Top, Kocide and Topsin M) and six phytochemicals (Nigella sativa, Azadirachta indica, Zingiber officinale, Cinnamomum verum, and Eucalyptus globulus) at three different concentrations were evaluated. Results indicated that maximum inhibition of mycellial growth was expressed by Fossil (5.24 mm) followed by Topsin M (7.13 mm), Axel (7.38 mm), Kocide (11.289 mm), Forum Top (18.79 mm), CabrioTop (25.01 mm). Among phyto-extracts, Datura stramonium (7.77 mm) showed maximum reduction in fungal growth followed by Nigella sativa (13.88 mm), Azadirachta indica (19.18 mm), Zingiber officinale (24.87 mm), Cinnamomum Verum (25.59 mm), and Eucalyptus globulus (36.13 mm) as compared to control (water). The current study, offers valuable insights into the prevalence and distribution of brown spot disease in District Okara, there by facilitating the development of focused management strategies to alleviate its impact on potato cultivation.
  • Genomic characterization of Puccinia triticina using molecular marker technology
    M. Usman, M. Habib, M. Sathish, S. Iqbal, J. Altaf, Z. Ahmad, A. ur Rehman, A. I. Khan, R. Maqbool, S. Hussain, F. Saleem, Z. Kashif, F. S. Awan
    Brazilian Journal of Biology, 2024
    Leaf rust, caused by Puccinia triticina, is the most common rust disease of wheat. The fungus is an obligate parasite capable of producing infectious urediniospores. To study the genetic structure of the leaf rust population 20 RAPD primers were evaluated on 15 isolates samples collected in Pakistan. A total of 105 RAPD fragments were amplified with an average of 7 fragments per primer. The number of amplified fragments varied from 1 to 12. GL Decamer L-07 and GL Decamer L-01 amplified the highest number of bands (twelve) and primer GL Decamer A-03 amplified the lowest number of bands i.e one. Results showed that almost all investigated isolates were genetically different that confirms high genetic diversity within the leaf rust population. Rust spores can follow the migration pattern in short and long distances to neighbor areas. Results indicated that the greatest variability was revealed by 74.9% of genetic differentiation within leaf rust populations. These results suggested that each population was not completely identical and high gene flow has occurred among the leaf rust population of different areas. The highest differentiation and genetic distance among the Pakistani leaf rust populations were detected between the leaf rust population in NARC isolate (NARC-4) and AARI-11and the highest similarity was observed between NARC isolates (NARC-4) and (NARC-5). The present study showed the leaf rust population in Pakistan is highly dynamic and variable.
  • Synthetic fungicides for controlling brown leaf spot of rice caused by Bipolaris oryzae
    H.M.U. Shaheen, N.A. Rajput, M. Atiq, L. Amrao, W.R. Arshad, G.A. Kachelo, M. Usman, M.F. Tahir
    International Journal of Biology and Chemistry, 2023
  • Exploration of computational approaches to predict the structural features and recent trends in α-amylase production for industrial applications
    Mohsin Shad, Naveed Hussain, Muhammad Usman, Muhammad W. Akhtar, Muhammad Sajjad
    Biotechnology and Bioengineering, 2023
  • Estimation of Biocidal Potential of Desert Phytopowders for the Management of Citrus Canker
    Muhammad Atiq Muhammad Atiq, Kiran Fatima Kiran Fatima, Nasir Ahmed Rajput, Shahbaz Talib Sahi, Akhtar Hameed, Muhammad Usman Muhammad Usman, Usama Ahmad, Adeel Sultan, Shahid Iqbal, Ahmad Nawaz
    Sains Malaysiana, 2023
  • Determination of biochemical baseline of resistance against bacterial leaf spot of chilli after application of plant defense activators
    Zaid Bin Tahir, Abuzar Hasnain
    Journal of Global Innovations in Agricultural Sciences, 2023
  • MORINGA OLEFRA PLANT BASED COPPER AND SILVER NANO PARTICLES AND ITS ANTIBACTERIAL ACTIVITY TOWARDS LEAF SPOT OF CHILLI CAUSED BY XANTHOMONAS CAMPESTRIS PV. VESICATORIA
    Tahreem Fatima, Nasir A. Rajput, Muhammad Usman, Ghalib A. Kachelo, Usama Ahmad, Asif M. Arif, Ahmad Nawaz, Muhmmad Kashif, Maryam Ashraf
    International Journal of Phytopathology, 2023
  • NEW Fangled Tactics Towards Cotton Leaf Curl Virus Disease A Review
    Muhammad Atiq, Muhammad Zeeshan Talib, Nasir Ahmed Rajput, Shahbaz Talib Sahi, Muhammad Aslam Khan, Muhammad Usman, Abdul Jabar, Muhammad Jalal Arif, Muhammad Dildar Gogi, Muhammad Ahsan Khan, Azeem Akram
    Journal of Natural Fibers, 2023
  • Efficacy of Green Synthesized Silver Based Nanomaterials Against Early Blight of Tomato Caused by Alternaria solani
    Muhammad Usman, Muhammad Atiq, Nasir Ahmed Rajput, Shahbaz Talib Sahi, Mohsin Shad, Nian Lili, Shahid Iqbal, Asif Mahmood Arif, Usama Ahmad, Khurram Shehzad Khan, Muhammad Asif, Fasih Ullah Haider
    Gesunde Pflanzen, 2023
  • Structural-Functional Characterization of Cytochrome b in bc1 and b6 f Complexes along with Polymorphic Analysis
    Mohsin Shad, Muhammad Usman, Qurratulann Afza Gardner
    Pakistan Journal of Zoology, 2022
  • GREEN SYNTHESIS OF SILVER AND COPPER NANOPARTICLES FROM LEAVES OF EUCALYPTUS GLOBULUS AND ASSESSMENT OF ITS ANTIBACTERIAL POTENTIAL TOWARDS XANTHOMONAS CITRI PV. CITRI CAUSING CITRUS CANKER
    M. ATIQ, H.M.R. MAZHAR, N.A. RAJPUT, U. AHMAD, A. HAMEED, A.M. LODHI, M. USMAN, A. NAWAZ, M. AMMAR, M. KHALID
    Applied Ecology and Environmental Research, 2022
  • DETERMINATION OF BACTERICIDAL POTENTIAL OF GREEN BASED SILVER AND ZINC NANOPARTICLES AGAINST BACTERIAL CANKER OF TOMATO
    Muhammad Atiq, Maryam Ashraf, Nasir Ahmed Rajput, Shahbaz Talib Sahi, Azeem Akram, Muhammad Usman, Shahid Iqbal, Ahmad Nawaz, Asif Mehmood Arif, Abuzar Hasnain
    Plant Protection, 2022
  • Entomopathogenic nematodes as biological control agent against Bactrocera zonata and Bactrocera dorsalis (Diptera: Tephritidae)
    Muhammad Usman, Waqas Wakil, David I. Shapiro-Ilan
    Biological Control, 2021
  • Impact Of Downy Mildew Disease Caused By Pseudoperonospora Cubensis On Cucumber Germplasm And Its Horticultural Attributes
    Sabir H. Khan, Iqra Mubin, Abdul Jabbar, Hafiz M.F. Shaheen, Kiran Fatima, Muhammad Usman, Naveed Aslam, Hafiz M.R. Mazhar
    Pakistan Journal of Phytopathology, 2021
  • ACTIVATION OF CAPSICUM ANNUUM L. DEFENSE SYSTEM AGAINST FUSARIUM WILT THROUGH PLANT ACTIVATORS AND THEIR IMPACT ON HORTICULTURAL ATTRIBUTES
    Muhammad Atiq, Sameen Adil, Nasir A. Rajput, Shahbaz T. Sahi, Muhammad Usman, Shahid Iqbal, Shahid A. Chand, Ahmad Nawaz, Asif M. Arif, Azeem Akram, Hamza Shahbaz
    International Journal of Phytopathology, 2021
  • ROLE OF EXOGENOUS APPLICATION OF ALPHA-TOCOPHEROL IN REDUCING LOW TEMPERATURE STRESS IN BELL PEPPER
    Muhammad Atiq, Sameen Adil, Nasir A. Rajput, Shahbaz T. Sahi, Akhtar Hameed, Abdul Jabbar, Muhammad Usman, Shahid Iqbal, Hamza Tariq, Hafiz M. R. Mazhar, Muhammad F. Ullah
    International Journal of Phytopathology, 2021
  • Nanoparticles: a safe way towards fungal diseases
    Muhammad Atiq, Isra Naeem, Shahbaz Talib Sahi, Nasir Ahmed Rajput, Ehsan Haider, Muhammad Usman, Hamza Shahbaz, Kiran Fatima, Ehtsham Arif, Abdul Qayyum
    Archives of Phytopathology and Plant Protection, 2020

RECENT SCHOLAR PUBLICATIONS

  • Bacterial biological control agents and resistance resources to enhance defense and morphological attributes of Capsicum annuum against Fusarium wilt
    M Usman, M Atiq, NA Rajput, M Sagheer, Y Xia
    Journal of Plant Pathology, 1-22 , 2026
    2026
  • Impact of silica nanoforms on the health and yield of crops
    M Usman, M Atiq, NA Rajput, M Shad, AM Arif
    Nanomaterials for Enhanced Plant-Based Food Production 1, 111-124 , 2025
    2025
    Citations: 1
  • Computational and experimental approaches to explore defense related enzymes conferring resistance in Fusarium infected chilli plants by regulating plant metabolism through …
    M Usman, M Atiq, NA Rajput, M Sagheer, Y Xia
    PLoS One 20 (1), e0309738 , 2025
    2025
    Citations: 14
  • Entomopathogenic fungi as alternatives to chemical acaricides: Challenges, opportunities and prospects for sustainable tick control
    M Rajput, MS Sajid, NA Rajput, DR George, M Usman, M Zeeshan, ...
    Insects 15 (12), 1017 , 2024
    2024
    Citations: 29
  • Advancements in plant gene editing technology: from construct design to enhanced transformation efficiency
    P Yuan, M Usman, W Liu, A Adhikari, C Zhang, V Njiti, Y Xia
    Biotechnology Journal 19 (12), e202400457 , 2024
    2024
    Citations: 26
  • Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
    M Shad, A Nazir, M Usman, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 266, 131310 , 2024
    2024
    Citations: 22
  • Efficacy of Green Synthesized Silver Based Nanomaterials Against Early Blight of Tomato Caused by Alternaria solani
    M Usman, M Atiq, NA Rajput, ST Sahi, M Shad, N Lili, S Iqbal, AM Arif, ...
    Journal of Crop Health 76 (1), 105-115 , 2024
    2024
    Citations: 37
  • DETECTION OF PHYTOPHTHORA NICOTIANAE INDUCED CITRUS GUMMOSIS BY THE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
    H AHMAD, NA RAJPUT, M ATIQ, GA KACHELO, M USMAN, H TARIQ, ...
    Pak. J. Bot 56, 5 , 2024
    2024
    Citations: 17
  • NEW fangled tactics towards cotton leaf curl virus disease A review
    M Atiq, MZ Talib, NA Rajput, ST Sahi, MA Khan, M Usman, A Jabar, ...
    Journal of Natural Fibers 20 (2), 2217364 , 2023
    2023
    Citations: 12
  • Exploration of computational approaches to predict the structural features and recent trends in α‐amylase production for industrial applications
    NH Mohsin Shad, M Usman, MW Akhtar, M Sajjad
    Biotechnology and Bioengineering 120, 2092-2116 , 2023
    2023
    Citations: 45
  • Management of damping-off disease of chili caused by Pythium aphanidermatum using synthetic chemicals.
    N Rajput, M Atiq, S Sahi, A Hameed, M Usman, A Nawaz, G Kachelo, ...
    Journal of Agricultural & Marine Sciences (JAMS) 28 (2) , 2023
    2023
  • Estimation of green synthesized silver and copper nano-particles for the management of leaf spot caused by Curvularia Lunata on brinjal.
    M Atiq, I Naeem, N Rajput, S Sahi, M Usman, S Iqbal, A Nawaz, H Ahmad
    Journal of Agricultural & Marine Sciences (JAMS) 28 (2) , 2023
    2023
  • Evaluation of chemicals and plant defense activators against red rot of sugarcane caused by Colletotrichum Falcatum.
    A Nawaz, M Atiq, S Sahi, N Rajput, M Usman, S Iqbal, G Kachelo, ...
    Journal of Agricultural & Marine Sciences (JAMS) 28 (2) , 2023
    2023
  • Management of Phytophthora spp. associated with citrus gummosis by using chemicals and nanoparticles.
    H Ahmad, N Rajput, M Atiq, S Sahi, G Kachelo, M Usman, A Nawaz, ...
    Journal of Agricultural & Marine Sciences (JAMS) 28 (2) , 2023
    2023
  • Moringa olefra plant based copper and silver nano particles and its antibacterial activity towards leaf spot of chilli caused by Xanthomonas campestris pv. vesicatoria.
    T Fatima, NA Rajput, M Usman, GA Kachelo, U Ahmad, AM Arif, A Nawaz, ...
    2023
    Citations: 11
  • Structural-Functional Characterization of Cytochrome b in bc1 and b6 f Complexes along with Polymorphic Analysis
    M Shad, M Usman, QA Gardner
    Pakistan Journal of Zoology 55 (2), 975 , 2023
    2023
    Citations: 17
  • Estimation of biocidal potential of desert phytopowders for the management of citrus canker
    M Atiq, K Fatima, NA Rajput, ST Sahi, A Hameed, M Usman, U Ahmad, ...
    Sains Malaysiana 52 (3), 757-770 , 2023
    2023
    Citations: 8
  • Induction of resistance in mungbean against cercospora leaf spot through plant defense activators
    WD Laraib Malik, Muhammad Atiq, Nasir Ahmed Rajput, Muhammad Usman, Azeem ...
    Agricultural Sciences Journal 5 (3), 28-34 , 2023
    2023
    Citations: 2
  • Synthetic fungices for controlling brown leaf spot of rice caused by Bipolaris oryzae
    HMU Shaheen, NA Rajput, M Atiq, L Amrao, WR Arshad, GA Kachelo, ...
    International Journal of Biology and Chemistry 16 (2), 33-42 , 2023
    2023
    Citations: 6
  • Determination of biochemical baseline of resistance against bacterial leaf spot of chilli after application of plant defense activators
    T Zaid, Bin, A Muhammad, R Nasir, Ahmed, A Azeem, A Asif, Mahmood, ...
    Journal of Global Innovations in Agricultural Sciences 11 (01), 61-67 , 2023
    2023
    Citations: 10

MOST CITED SCHOLAR PUBLICATIONS

  • Exploration of computational approaches to predict the structural features and recent trends in α‐amylase production for industrial applications
    NH Mohsin Shad, M Usman, MW Akhtar, M Sajjad
    Biotechnology and Bioengineering 120, 2092-2116 , 2023
    2023
    Citations: 45
  • Nanoparticles: A safe way towards fungal diseases
    M Atiq, I Naeem, ST Sahi, NA Rajput, E Haider, M Usman, H Shahbaz, ...
    Archives of Phytopathology and Plant Protection 53 (17-18), 781-792 , 2020
    2020
    Citations: 39
  • Efficacy of Green Synthesized Silver Based Nanomaterials Against Early Blight of Tomato Caused by Alternaria solani
    M Usman, M Atiq, NA Rajput, ST Sahi, M Shad, N Lili, S Iqbal, AM Arif, ...
    Journal of Crop Health 76 (1), 105-115 , 2024
    2024
    Citations: 37
  • GREEN SYNTHESIS OF SILVER AND COPPER NANOPARTICLES FROM LEAVES OF EUCALYPTUS GLOBULUS AND ASSESSMENT OF ITS ANTIBACTERIAL POTENTIAL TOWARDS XANTHOMONAS CITRI PV. CITRI CAUSING …
    M ATIQ, M. – MAZHAR, H. M. R. – RAJPUT, N. A. – AHMAD, U. – HAMEED, A ...
    APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 20 (3), 2205-2213 , 2022
    2022
    Citations: 30
  • Entomopathogenic fungi as alternatives to chemical acaricides: Challenges, opportunities and prospects for sustainable tick control
    M Rajput, MS Sajid, NA Rajput, DR George, M Usman, M Zeeshan, ...
    Insects 15 (12), 1017 , 2024
    2024
    Citations: 29
  • Advancements in plant gene editing technology: from construct design to enhanced transformation efficiency
    P Yuan, M Usman, W Liu, A Adhikari, C Zhang, V Njiti, Y Xia
    Biotechnology Journal 19 (12), e202400457 , 2024
    2024
    Citations: 26
  • Investigating the effect of SUMO fusion on solubility and stability of amylase-catalytic domain from Pyrococcus abyssi
    M Shad, A Nazir, M Usman, MW Akhtar, M Sajjad
    International Journal of Biological Macromolecules 266, 131310 , 2024
    2024
    Citations: 22
  • Mango anthracnose: global status and the way forward for disease management
    S Iqbal, MA Khan, M Atiq, NA Rajput, M Usman, A Nawaz, GA Kachelo, ...
    Journal of Innovative Sciences 8 (2), 222-235 , 2022
    2022
    Citations: 18
  • DETECTION OF PHYTOPHTHORA NICOTIANAE INDUCED CITRUS GUMMOSIS BY THE LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
    H AHMAD, NA RAJPUT, M ATIQ, GA KACHELO, M USMAN, H TARIQ, ...
    Pak. J. Bot 56, 5 , 2024
    2024
    Citations: 17
  • Structural-Functional Characterization of Cytochrome b in bc1 and b6 f Complexes along with Polymorphic Analysis
    M Shad, M Usman, QA Gardner
    Pakistan Journal of Zoology 55 (2), 975 , 2023
    2023
    Citations: 17
  • A way forward towards the management of chilli anthracnose-a review
    M Atiq, NA Rajput, ST Sahi, A Akram, M Usman, GA Kachelo, H Ahmad, ...
    Agricultural Sciences Journal 4 (1), 1-10 , 2022
    2022
    Citations: 15
  • Computational and experimental approaches to explore defense related enzymes conferring resistance in Fusarium infected chilli plants by regulating plant metabolism through …
    M Usman, M Atiq, NA Rajput, M Sagheer, Y Xia
    PLoS One 20 (1), e0309738 , 2025
    2025
    Citations: 14
  • NEW fangled tactics towards cotton leaf curl virus disease A review
    M Atiq, MZ Talib, NA Rajput, ST Sahi, MA Khan, M Usman, A Jabar, ...
    Journal of Natural Fibers 20 (2), 2217364 , 2023
    2023
    Citations: 12
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  • Impact of downy mildew disease caused by Pseudoperonospora cubensis on cucumber germplasm and its horticultural attributes.
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