M.Sc. (Microbiology), Ph.D. (Microbiology), MBA (Finance)
RESEARCH, TEACHING, or OTHER INTERESTS
Applied Microbiology and Biotechnology, Biotechnology, Multidisciplinary, Process Chemistry and Technology
24
Scopus Publications
Scopus Publications
Effect of chemical, physical, and biological pre-treatment of food wastes on bio-hydrogen production by dark anaerobic fermentation under mesophilic conditions Khushboo Swapnil Bhurat, Tushar Banerjee, Prakash Vasantrao Bobde, Swapnil Sureshchandra Bhurat Energy Sources Part A Recovery Utilization and Environmental Effects, 2023 With an overall aim of utilizing lignocellulosic food waste as feedstock for anaerobic fermentation and optimizing the reaction for improving energy output, a two-stage anaerobic dark fermentation reactor was set up. This study discusses the evaluation of different pre-treatment methods used to pre-treat the food waste before using it as a substrate for anaerobic fermentation. Five different techniques from physical, chemical, and biological pre-treatment methods, namely, autoclaving, acid pre-treatment, alkali pre-treatment, aeration, and fungal pre-treatment were used for pre-treatment of the substrate. The analysis was also evaluated by the multilevel categoric factorial design of the experiment model. The experiment found that all five pre-treatments improved the hydrogen and methane yield from the reaction. More precisely, fungal pre-treatment shows an almost 3.8-fold improvement in hydrogen yield compared to control conditions and a 1.7-fold increase in methane yield compared to control. The statistical analysis showed that the reaction time duration (day) has a more significant impact on the results than the pre-treatment technique. The model’s F-Values of 24.14 for hydrogen yield and 44.34 for methane yield indicate substantially. However, the actual hydrogen and methane yields are in good accord with the DOE predicted results.
Medium optimization for submerged fermentative production of β-cyclodextrin glucosyltransferase by isolated novel alkalihalophilic Bacillus sp. NCIM 5799 using statistical approach P. Solanki, T. Banerjee Letters in Applied Microbiology, 2022 β-cyclodextrin glucosyltransferase (β-CGTase) is an essential enzyme to catalyse the biotransformation of starch into β-cyclodextrins (β-CD). β-CD has widespread applications in the biomedical, pharmaceutical and food industries. The present study focused on β-CGTase production using an efficient natural microbial strain and statistical production optimization for enhanced production. The isolated organism Bacillus sp. NCIM 5799 was found to be 5 μm short bacilli under FE-SEM and alkalihalophilic in nature. The β-CGTase production was optimized using a combination of Plackett–Burman design (PBD) and Central Composite Design—Response Surface Methodology (CCD-RSM). On PBD screening Na2CO3, peptone and MgSO4.7H2O were found to be significant for optimal β-CGTase production, whereas the soluble starch and K2HPO4 concentrations were found to be nonsignificant for β-CGTase production. The significant factors obtained after PBD were further optimized using CCD-RSM design. Peptone was found to have a significant interaction effect with Na2CO3, and MgSO4·7H2O and Na2CO3 exhibited a significant effect on the production of CGTase. The production of β-CGTase was enhanced in the presence of peptone (3%) and Na2CO3 (0·8%). CGTase production obtained was 156·76 U/ml when optimized using CCD-RSM. The final optimized medium (RSM) shows 7·7- and 5·4-fold high productions as compared to un-optimized and one factor at a time production media.
CONVENTIONAL PRODUCTION OPTIMIZATION OF CYCLODEXTRIN GLUCOSYL TRANSFERASE BY A NOVEL ISOLATE OF BACILLUS SP. PBS1 FROM POTATO RHIZOSPHERE Preetibala Solanki, Pushpendra Awadhiya, Tushar Banerjee Journal of Microbiology Biotechnology and Food Sciences, 2022 The cyclodextrin glucosyltransferase enzyme (CGTase) is an industrially crucial enzyme for the production of β-cyclodextrin (β-CD). CGTase has a high propensity to produce a mixture of cyclodextrins (CDs). From the industrial perspective, a CGTase that produces only one type of CD is of critical importance. Bacillus sp. PBS1 produced CGTase that converts starch solely into β-CD. The isolated strain PBS1 was found to close similarity with alkaliphilic Bacillus sp. based on biochemical, morphological, and phylogenetic analysis of its 16s rRNA gene sequencing. The selection and optimization of media ingredients are warranted for the best possible production of β-CD. These steps were carried out by conventional optimization strategies. The presence of glucose, maltose, lactose, sucrose, galactose, mannitol, nitrates, urea, metal salts, and K2HPO4 led to the suppression of CGTase production. The improved enzyme production was observed in peptone, soluble starch, magnesium sulfate, and Na2CO3. The organism produces maximum CGTase (93.42 ± 2.4 U/ml) at 96-hour incubation in the optimized production medium containing 8% starch, 2% peptone, 0.06% MgSO4.7H2O, 0.5% Na2CO3, and having pH of 9.3. The optimization of the medium led to ~16% improvement in CGTase production by Bacillus sp. PBS1.
Influence of small amount of hydrogen fuel on spark-ignition engine characteristics through H-CNG fuel blend and its comparative study Swapnil Sureshchandra Bhurat, Khushboo Swapnil Bhurat, Tushar Banerjee, Ram Kunwer, Santhosh Kumar Gugulothu, Subrahmanya Ranjit Pasupuleti Energy Sources Part A Recovery Utilization and Environmental Effects, 2022 An experimental study was performed on single-cylinder, four-stroke, and spark ignition engines to understand the impact of a small quantity of hydrogen fractions in CNG fuel on an engine’s performance and emission characteristics. The two blends of H-CNG in which hydrogen fuel was used were 0.20 kg/hr and 0.25 kg/hr mass flow rate at varying engine rpm. The hydrogen enrichment increases the BTE by 3.54% and 4.94%, for HCNG (0.20 kg/hr) and HCNG (0.25 kg/hr), relative to CNG operation. Similarly, the hydrogen enrichment reduces the BSFC with higher margin due to its increment in calorific value compared to CNG operation. The H-CNG fuel blend has shown severe decrement in HC, CO, CO2, and O2 emissions compared to gasoline and CNG fuel. However, NOx emissions were a concern in CNG-fueled engine. The CNG-fueled engine has produced almost 32% of higher NOx emissions than gasoline. However, adding H2 fuel to CNG decreased NOx emissions and brought it nearly equal to the gasoline-fueled engine emissions at overall engine rpm. Overall, from a future perspective, the H-CNG mode of operation with optimum proportion of Hydrogen-CNG can be considered a suitable fuel blend to control engine emissions without compromising engine performance of conventional SI engines.
Substrate carriers for C-1(2)-dehydrogenation of 6-methylene androstenedione to exemestane by growing and immobilized arthrobacter simplex NCIM 2449 Prachi Patil, Rajesh Sharma, Tushar Banerjee, Shridhar Patil Asian Journal of Pharmaceutical and Clinical Research, 2017 Objective: Permeability of hydrophobic steroid substrates across cell membrane is a critical factor during microbial bioconversion. To increase substrate intake, the feasibility of some organic solvents and emulsifiers as substrate carrier on the bioconversion of 6-methylene androstenedione to exemestane was assessed.Methods: Androstenedione, a commonly available steroid precursor, was chemically converted 6-methylene androstenedione. The time course of exemestane accumulation was estimated after addition of 6-methylene androstenedione dissolved in some organic solvents or dispersed with emulsifiers by growing and immobilized cells of Arthrobacter simplex NCIM 2449 in shake flask cultures. Results: The use of substrate carriers for addition of 6-methylene androstenedione enhanced the bioconversion several folds. With growing bacterium in triplicate flasks, a peak mol % bioconversion recorded was- ethanol (67.25, 72 h); soybean oil + tween 80 (50.37, 48 h); acetone (38.84, 48 h); soybean oil (38.36, 48 h); lecithin (32.73, 48 h), methanol (32.71, 48 h) and tween 80 (10.37, 48 h). As compared to the growing cells, the bioconversion with Ca-alginate immobilized cells was delayed and peak mol % bioconversion was recorded as ethanol (60.78, 120 h); soybean oil + tween 80 (42.98, 120 h); methanol (40.50, 72 h); soybean oil (38.36, 48 h); acetone (31.18, 72h ) and lecithin (33.67, 120 h); tween 80 (13.87, 120 h).Conclusion: The use of substrate carriers for addition of 6-methylene androstenedione increased the permeability of substrate and may be used to increase the yield of exemestane and reduce incubation time.
Bioconversion of 16-dehydropregnenolone acetate to exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363 PUSHPENDRA AWADHIYA, TUSHAR BANERJEE, SHRIDHAR PATIL Polish Journal of Microbiology, 2017 Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.
Inhibition of Aβ(1-42) oligomerization, fibrillization and acetylcholinesterase activity by some anti-inflammatory drugs: An in vitro study Hamendra S. Parmar, Anshul Assaiya, Renuka Agrawal, Shalini Tiwari, Iram Mufti, Neha Jain, Elangovan Manivannan, Tushar Banerjee, Anil Kumar Anti Inflammatory and Anti Allergy Agents in Medicinal Chemistry, 2016 BACKGROUND Number of contradictory reports are available on the effects of antiinflammatory drugs on Alzheimer's disease (AD) including beneficial, adverse and stage dependent effects. We provide insights of the effects exerted by some anti-inflammatory drugs on the chemistry of AD. METHODS Three different doses of dexamethasone (0.015, 0.030, 0.060 μM), piroxicam (5, 7.5, 10 μM), indomethacin (1, 1.25, 1.50 μM), diclofenac (0.6, 0.8, 1.0 μM), aspirin (90, 120, 150 μM) and celecoxib (30, 45, 60 μM) were used. Rivastigmine, methylene blue and butylated hydroxyanisole were used as standard drug, oligomerization inhibitor and antioxidant, respectively. Oligomerization and fibrillization reactions were performed using Aβ1-42 peptides. Results-Indomethacin and aspirin mainly inhibited oligomerization, while rivastigmine and piroxicam inhibited fibrillization. Diclofenac and celecoxib inhibited both oligomerization and fibrillization almost equally. Dexamethasone showed poor efficiency on both the processes, but exert comparably more inhibition of oligomerization than fibrillization. Inhibition of acetylcholinesterase activity was also potent and was in the following order: celecoxib> piroxicam> diclofenac> aspirin> indomethacin> dexamethasone. Strong radical scavenging (More than 50%) activity was showed by indomethacin and aspirin for NO radicals. CONCLUSION Present study consistently revealed that anti-inflammatory drugs have potential to Modulate chemistry of AD progression. Inclusion of anti-inflammatory drugs in low doses along with routine therapies may provide therapeutically and economically more efficient therapies for AD. However, further studies are warranted, because the overall therapeutic effect seems to be the function of stage of disease, dose of drug, main underlying mechanism of action(s).
Spectroscopic and surface studies of catalytically active Zn 1-xMnxFe2O4 (X= 0, 0.25, 0.50, 0.75 and 1) ferrospinel systems Indian Journal of Chemistry Section A Inorganic Physical Theoretical and Analytical Chemistry, 2004
