She is an IV Associate Professor at the Department of Veterinary Medicine at the Federal University of Paraná (UFPR). Conducts studies in Veterinary Medicine, with emphasis on Diagnostic Imaging, working on the following topics: ultrasonography, radiology, computed tomography, dogs, cats, and exotic pets. She has already concluded the supervision of seventeen master's students and six Ph.D. students. Advises in the master's and Doctorate in the Post-Graduate Program in Veterinary Sciences - CAPES 6. She is an Imaging Diagnosis resident advisor in the Veterinary Hospital for HV UFPR - MEC. It currently presents several articles published in national and international journals, and abstracts published in symposiums, seminars, and conferences. Coordinator and Head of Chief the Diagnostic Imaging Service - HV UFPR. Approved and certificated by the Brazilian College of Veterinary Radiology.
EDUCATION
1991-1995: Doctor in Veterinary Medicine
UEL – Londrina University, School of Veterinary Medicine. Londrina, Paraná, Brazil
1998 – 2001: Master of Science (Core: Surgical Veterinary Department/ Option Diagnostic Imaging)
Dissertation: “Ultrasonographic evaluation of gastrointestinal tract in healthy cats and in cats with gastrointestinal diseases”.
São Paulo University, São Paulo, Brazil.
2001 – 2004: PhD (Core: Surgical Veterinary Department/ Option Diagnostic Imaging)
Thesis: “Use of ultrasonography in the diagnostic approach of dogs suspected of digestive neoplasm (liver, intestines and pancreas)”.
São Paulo University, São Paulo, Brazil.
Early Diagnosis of Chronic Kidney Disease in Asymptomatic Cats - Association of Renal Doppler Resistive Index with Elevated Symmetric Dimethylarginine Rafaelle Cristine Dea Gerez, Fabiana Rodrigues de Freitas, Rosangela Locatelli Dittrich, Camila Marinelli Martins, Tilde Rodrigues Froes Acta Scientiae Veterinariae, 2026 Background: Chronic kidney disease (CKD) in cats often progresses silently, making early diagnosis challenging. Symmetric dimethylarginine (sSDMA) is a sensitive biomarker for early renal dysfunction, while the resistive index (RI), obtained via Doppler ultrasonography, reflects renal vascular resistance. Combining these tools may improve early CKD detection. This study aimed to evaluate the correlation of RI with sSDMA in asymptomatic cats. Materials, Methods & Results: A prospective, blinded cohort study assessed the correlation between renal RI and sSDMA in 101 clinically asymptomatic cats. Examinations were performed without sedation, with manual restraint, and approved by the Federal University of Paraná's Animal Ethics Committee. Triplex Doppler ultrasonography used a GE Logic 5 machine with a 7.5-12 MHz linear transducer. B-mode assessed renal morphology, followed by color and pulsed Doppler for vasculature and interlobar arteries. RI was automatically calculated from three waveforms per kidney. An RI ≥0.59 (left) or ≥0.54 (right) was considered elevated. Blood samples were collected from the jugular vein. Creatinine and sSDMA were measured at IDEXX Laboratories (São Paulo, Brazil) using liquid chromatography-mass spectrometry. Urine was collected via cystocentesis. sSDMA values ≥ 14 μg/dL were considered elevated. Cats were grouped by sSDMA levels. Statistical analysis used SPSS 21.0, with P < 0.05 set as significant. Reliable RI measurements were obtained in 91 cats; 10 were excluded due to poor waveform acquisition (e.g., patient movement, panting, poor corticomedullary definition). Mean RI was 0.61 ± 0.07 (left) and 0.62 ± 0.07 (right), with no significant difference. Increased RI was seen in 55.9% (47/84) of left kidneys and 86% (74/86) of right kidneys. Elevated sSDMA was detected in 14.9% (15/101) of cats. A statistically significant correlation was found between elevated RI in the right kidney and increased sSDMA (P = 0.001), but not the left (P = 0.246). Creatinine also positively correlated with elevated sSDMA (P = 0.022), supporting the link between renal hemodynamic changes and early biochemical markers. These results suggest RI, particularly in the right kidney, may be a useful non-invasive parameter for early CKD detection in cats, especially with sSDMA. Discussion: While renal ultrasonography is common in cats, few studies explore RI's diagnostic potential in asymptomatic animals. Doppler ultrasonography was feasible but sometimes limited by patient stress, obesity, and altered renal morphology, affecting waveform quality. No significant difference was found between right and left kidney RI values, though some cats showed inter-kidney discrepancies, possibly indicating unilateral disease. Mean RI values were normal but near early CKD values.The significant correlation between elevated RI in the right kidney and increased sSDMA was not observed in the left. The asymmetry is unclear, but extrarenal factors and comorbidities might influence both RI and sSDMA. Despite limitations, creatinine also correlated positively with sSDMA, supporting its reliability in early renal assessment. RI should not be a standalone CKD screening method but a complementary tool, especially with sSDMA. Bilateral assessment is recommended, and interpretation should be cautious with morphological abnormalities or poor patient cooperation. Overall, RI measurement is feasible in unsedated cats and may aid early detection of renal changes in subclinical disease. Keywords: feline, resistive index, doppler, kidney, SDMA.
Right Ventricular Longitudinal Function in the Assessment of Fluid Responsiveness in Healthy Dogs Stephany Buba Lucina, Eloisa Muehlbauer, Vinícius Bentivóglio Costa Silva, Matheus Folgearini Silveira, Marcela Wolf, et al. Acta Scientiae Veterinariae, 2024 Background: An essential component in the treatment of critically ill patients is volume replacement, but excessive intravascular fluid administration is associated with increased morbidity and mortality. Therefore, this intervention is only beneficial in patients who are fluid responsive, that is, when the individual has an increase in at least 15% of the stroke volume after a volume challenge administration. In addition, one of the determinants of fluid responsiveness is the systolic function, associated with the dynamic interactions between intravascular volume (preload) and vascular tone (afterload). Within this context, the aim of this study is to evaluate if conventional and advanced echocardiographic parameters of right ventricular longitudinal systolic function can assess fluid responsiveness in healthy spontaneously breathing dogs. The hypothesis was that some of these parameters would differ between responsive and non-responsive animals and could be used as a complementary measure for assessment of fluid responsiveness. Materials, Methods & Results: This is a prospective study with 22 healthy dogs over 1 year of age included. The animals were referred to the Veterinary Medical Teaching Hospital of the Federal University of Paraná (UFPR) for elective neutering procedure. All dogs underwent conventional and advanced echocardiographic examination before and after administration of a volume challenge with 10 mL/kg lactate ringer intravenously for 20 min. The parameters evaluated were the fractional area change (FAC), the tricuspid annular plane systolic excursion (TAPSE), free wall longitudinal strain (LSt), and tissue motion annular displacement (TMAD). Based on the aortic velocity integral time variation, 31.82% of dogs were considered responsive and 68.18% were non-responsive to the volume challenge. For conventional echocardiography, TAPSE (mm/kg) > 1.35 (P = 0.018) had a good combination of sensitivity (85.71%) and specificity (86.67%), area under the curve value (0.814) and a relative smaller gray zone interval (1.04-1.66) for the identification of responsive dogs before volume challenge. Although, TAPSE (mm/m2) was also higher in the responsive dogs (P = 0.023) before volume challenge. As for advanced echocardiography, the TMAD (mm/kg) > 0.75 (P = 0.010) after volume challenge had the best combination of sensitivity (85.71%) and specificity (86.67%), area under the curve value (0.852) and a relative smaller gray zone interval (0.57-0.92) for the identification of responsive dogs. The LSt (%) and TMAD (mm/m2) were also significantly higher in responsive dogs (P = 0.031; P = 0.011) after volume challenge. Discussion: Some of the echocardiographic parameters for assessing right ventricular systolic function differed between responsive and non-responsive dogs proving to been useful in the fluid responsiveness evaluation. To the author's knowledge, this is the 1st study to investigate the right ventricular systolic function in the assessment of fluid responsiveness in healthy, spontaneously breathing dogs. TAPSE and TMAD showed some advantages in terms of execution, although TAPSE (mm/kg) > 1.35 before the volume challenge had the power to predict fluid responsiveness without the need of a fluid administration, which can be seen as a great advantage, especially for patients with volume overload. However, it is also necessary to perform this type of evaluation in individuals with hemodynamic alterations to allow better understand the applicability of these techniques. Keywords: canine, longitudinal strain, stroke volume, tissue motion annular displacement.
The use of agitated saline as contrast agent in a contrast-enhanced cystosonography for detection of urinary bladder rupture in animal cadavers Eloisa Muehlbauer, André Saldanha, Elaine M. Gil, Thais L. Alves, Juan C. M. Duque, et al. Journal of Veterinary Emergency and Critical Care, 2023 OBJECTIVE To determine the value of microbubble contrast cystosonography in the diagnosis of bladder rupture in animals. DESIGN Prospective, method comparison study from November 2019 to October 2020. SETTING University teaching hospital. ANIMALS Thirty-four ethically sourced cadavers of dogs, rats, and rabbits. INTERVENTIONS In a prospective and blinded study, the cadavers were divided into 2 randomized groups: with bladder rupture (CR), and without bladder rupture (SR). Urinary catheterization was performed in all cadavers. Through the urethral catheter, bladders in CR group were ruptured using a rigid stainless steel guide wire. Microbubble contrast was infused into the bladder through the urethral catheter, while a single, blinded observer sonographically assessed the bladder. The time to diagnosis and the number of attempts needed for diagnosis were recorded. MEASUREMENTS AND MAIN RESULTS The study included cadavers of 16 female Wistar rats, 6 female dogs, 11 male dogs, and 1 male rabbit. Time to diagnosis in dogs (2.25 ± 0.91 min) was statistically higher when compared to rats (1.15 ± 0.75 min; P = 0.03). Of the 34 cases, incorrect diagnosis of bladder rupture was made in only 2 dogs (6%), indicating a diagnostic sensitivity of 88.88%, specificity of 100%, and an accuracy of 94%. The positive predictive value was 1 and the negative predictive value was 0.9. CONCLUSIONS Our study showed that the described method is accurate, sensitive, and specific for the detection of bladder rupture in animal cadavers of different species, size, and sex.
Comparison of radiographic methods for detecting radiolucent uroliths in dogs Luciano Alves Faria, Adriana Érica Wilkes Burton Meirelles, Tilde Rodrigues Froes, Thassila Caccia Feragi Cintra, Daniel Peixoto Pereira, et al. Plos One, 2022 The purpose of this study was to evaluate and compare positive cystography techniques at 5%, 10%, and 20%, as well as three different double-contrast protocols for detecting radiolucent uroliths with a diameter of less than 3.0 mm in dogs. Six cadavers were used, one was selected at random to represent the negative control, and the others were submitted to urolith implantation in the bladder by urethral catheter. Three radiology professionals blindly accessed ventrodorsal and -lateral projections of each test. Contrast at 20% showed greater diagnostic sensitivity, but with greater difficulty identifying the number and size of the uroliths. Consequently, double-contrast techniques are better and should be used for diagnostic and therapeutic planning. Sensitivity and specificity tests demonstrated that positive 5% cystography and different concentrations of double contrast obtained better results in terms of sensitivity and specificity. However, due to the presence of a greater amount of artifacts in the 5% cystography, it is suggested that double contrast is used for this purpose, especially with the removal of contrast excess (protocol 2).
Rickets in a crab-eating fox (Cerdocyon thous) Alana Macena Anselmo, Andre Saldanha, Eloisa Muelbahuer, Danielle Buch, Mariana Reffatti De Oliveira, et al. Acta Scientiae Veterinariae, 2020
Vertebral left atrial ventral: a new prognostic marker in dogs with mitral valve disease T Beluque, M Endrighi, SB Lucina, A Rodrigues, JB Braz, E Zacché, ... American Journal of Veterinary Research 1 (aop), 1-9 , 2026 2026
Early Diagnosis of Chronic Kidney Disease in Asymptomatic Cats-Association of Renal Doppler Resistive Index with Elevated Symmetric Dimethylarginine R Gerez, FR de Freitas, RL Dittrich, CM Martins, TR Froes Acta Scientiae Veterinariae 54 , 2026 2026
Presumptive diagnosis of gastrointestinal pythiosis in a dog with pyloric obstruction: case report. AHF dos Santos, AS Cruz, GT de Abreu, GJ Salles, VMA Pasqual, ... 2025
Diagnóstico presuntivo de pitiose gastrointestinal em cão com obstrução pilórica: relato de caso AHF dos Santos, AS Cruz, GT de Abreu, GJ Salles, VMA Pasqual, ... Pubvet 19 (11), e1862-e1862 , 2025 2025
Classification of Left Atrial Enlargement in Canine Radiographs Using Artificial Intelligence GDM Restani, LTDBN Jaworski, TR Froes, LF De Oliveira IEEE Access 13, 191609-191618 , 2025 2025
Association of renal ultrasonographic findings with elevated serum symmetric dimethylarginine in asymptomatic cats RCD Gerez, FR de Freitas, RL Dittrich, CM Martins, TR Froes Archives of Veterinary Science 30 (3) , 2025 2025
Tomographic and radiographic evaluation of stenosis/collapse of the left main bronchus in a pug dog: case report. A Longo Filho, LB de Oliveira, CAN Castanho, TR Froes 2025
Avaliação tomográfica e radiográfica de estenose/colapso do brônquio principal esquerdo em cão da raça Pug: Relato de caso A Longo Filho, LB de Oliveira, CAN Castanho, TR Froes Pubvet 19 (02), e1726-e1726 , 2025 2025
Inteligência artificial aplicada à radiologia veterinária LT Nery-Brito, TR Fróes, LF Oliveira, FA Souza, PEB Néspoli 2025
Right Ventricular Longitudinal Function in the Assessment of Fluid Responsiveness in Healthy Dogs SB Lucina, E Muehlbauer, VBC Silva, MF Silveira, M Wolf, JP dos Santos, ... Acta Scientiae Veterinariae 52 , 2024 2024
EVALUATION OF THE EFFECTIVENESS OF TWO NEURAL NETWORKS (RESNET50V2 AND INCEPTION V3) IN IDENTIFYING LEFT ATRIAL ENLARGEMENT IN ADULT DOGS. L JAWORSKI, G RESTANI, L OLIVEIRA, TR FROES ARCHIVES OF VETERINARY SCIENCE 29 (3) , 2024 2024
Comparison Between Echocardiographic and Computed Tomographic Measurements of the Right Heart in Healthy Dogs. VBC Silva, SB Lucina, D Buch, E Muehlbauer, TR Froes, M Wolf, ... Acta Veterinaria Eurasia 50 (1) , 2024 2024
Umbilical artery Doppler measurements in last week of pregnancy and correlation with vitality of newborn lambs APB Busch, IS Compagnoni, GF da Cruz Filho, LI Borges, ... Acta Scientiae Veterinariae 51 , 2023 2023
Ultrasound-guided cystocentesis in dogs and cats-epidemiological study of risk factors and complications E Muehlbauer, AF Saldanha, EMU Gil, MS Sombrio, BR Sales, ... Acta Scientiae Veterinariae 51 , 2023 2023
Calcinosis circumscripta in the foot pad of a dog: case report. LB de Oliveira, C Konkel, KT Lewandowski, L Barazetti, ML Orsi, ... 2023
Calcinose circunscrita no coxim plantar de um cão: Relato de caso LLB de Oliveira, C Konkel, KT Lewandowski, L Barazetti, ML Orsi, ... Pubvet 17 (06), e1399-e1399 , 2023 2023 Citations: 1
Accuracy and reliability of tele‐ultrasonography in detecting gastrointestinal obstruction in dogs and cats M Silvestre Sombrio, W Mai, D Buch, G Costa Grotti, M Gabriela Luciani, ... Journal of Small Animal Practice 64 (6), 367-374 , 2023 2023 Citations: 3
The use of agitated saline as contrast agent in a contrast‐enhanced cystosonography for detection of urinary bladder rupture in animal cadavers E Muehlbauer, A Saldanha, EM Gil, TL Alves, JCM Duque, TR Froes Journal of Veterinary Emergency and Critical Care 33 (3), 327-331 , 2023 2023 Citations: 4
TÓPICOS AVANÇADOS NO DIAGNÓSTICO E EPIDEMIOLOGIA DE UROABDOME EM PEQUENOS ANIMAIS E Muehlbauer 2023
Characterization of Doppler spectrum of hepatic veins in dogs with pulmonary hypertension VBC Silva, TR Froes, M Wolf, SB Lucina, MG Sousa Research in veterinary science 150, 131-136 , 2022 2022 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Canine fetal heart rate: do accelerations or decelerations predict the parturition day in bitches? EMU Gil, DAA Garcia, AT Giannico, TR Froes Theriogenology 82 (7), 933-941 , 2014 2014 Citations: 70
Ultrasonography of small intestinal obstructions: a contemporary approach DAA Garcia, TR Froes, R Vilani, SD Guérios, A Obladen Journal of Small Animal Practice 52 (9), 484-490 , 2011 2011 Citations: 60
In utero development of the fetal intestine: Sonographic evaluation and correlation with gestational age and fetal maturity in dogs EMU Gil, DAA Garcia, TR Froes Theriogenology 84 (5), 681-686 , 2015 2015 Citations: 59
Identification of three distinguishable phenotypes in golden retriever muscular dystrophy A Morini Genetics and Molecular Research , 2009 2009 Citations: 53
The use of Doppler evaluation of the canine umbilical artery in prediction of delivery time and fetal distress AT Giannico, EMU Gil, DAA Garcia, TR Froes Animal Reproduction Science 154, 105-112 , 2015 2015 Citations: 49
Proven cardiac changes during death-feigning (tonic immobility) in rabbits ( Oryctolagus cuniculus ) AT Giannico, L Lima, RR Lange, TR Froes, F Montiani-Ferreira Journal of Comparative Physiology A 200 (4), 305-310 , 2014 2014 Citations: 43
Continuous transversus abdominis plane block for analgesia in three dogs with abdominal pain FAV Freitag, VL Bozak, MPW do Carmo, TR Froes, JCM Duque Veterinary anaesthesia and analgesia 45 (4), 581-583 , 2018 2018 Citations: 42
Early results on canine fetal kidney development: Ultrasonographic evaluation and value in prediction of delivery time EMU Gil, DAA Garcia, AT Giannico, TR Froes Theriogenology 107, 180-187 , 2018 2018 Citations: 34
Determination of normal echocardiographic, electrocardiographic, and radiographic cardiac parameters in the conscious New Zealand white rabbit AT Giannico, DAA Garcia, L Lima, FA de Lara, CAC Ponczek, GC Shaw, ... Journal of Exotic Pet Medicine 24 (2), 223-234 , 2015 2015 Citations: 32
Effects of propofol on the electrocardiogram and systolic blood pressure of healthy cats pre‐medicated with acepromazine GG Pereira, MHMA Larsson, FL Yamaki, EC Soares, RJ Yamato, ML Neto, ... Veterinary anaesthesia and analgesia 31 (3), 235-238 , 2004 2004 Citations: 30
Importance of fasting in preparing dogs for abdominal ultrasound examination of specific organs DAA Garcia, TR Froes Journal of Small Animal Practice 55 (12), 630-634 , 2014 2014 Citations: 29
Assessment of umbilical artery flow and fetal heart rate to predict delivery time in bitches AT Giannico, DAA Garcia, EMU Gil, MG Sousa, TR Froes Theriogenology 86 (7), 1654-1661 , 2016 2016 Citations: 25
Congenital heart disease in dogs: a retrospective study of 95 cases SB Lucina, AP Sarraff, M Wolf, VBC Silva, MG Sousa, TR Froes Topics in companion animal medicine 43, 100505 , 2021 2021 Citations: 23
Use of B-mode ultrasonography for fetal sex determination in dogs EMU Gil, DAA Garcia, AT Giannico, TR Froes Theriogenology 84 (6), 875-879 , 2015 2015 Citations: 23
A história da ultrassonografia veterinária em pequenos animais MP dos Reis Seoane, DAA Garcia, TR Froes Archives of Veterinary Science 16 (1), 54-61 , 2011 2011 Citations: 23
Errors in abdominal ultrasonography in dogs and cats DAA Garcia, TR Froes Journal of small animal practice 53 (9), 514-519 , 2012 2012 Citations: 22
Window collisions by birds in Brazil: Epidemiologic factors and radiographic and necropsy assessments GA Fornazari, A Saldanha, RR Lange, T Froes, D Klem Jr, BA Moore, ... Journal of Avian Medicine and Surgery 35 (3), 313-324 , 2021 2021 Citations: 21
Avaliação ultra-sonográfica e pelo Doppler colorido do carcinoma de células transicionais da bexiga em cães TR Fróes, M Iwasaki, AG Campos, LN Torres, MLZ Dagli Arquivo Brasileiro de Medicina Veterinária e Zootecnia 59 (6), 1400-1407 , 2007 2007 Citations: 19
Ultrassonografia do trato gastrointestinal TR Froes Carvalho CF. Ultra-sonografia em pequenos animais. São Paulo: Roca, 147-64 , 2004 2004 Citations: 18
Ultrassonografia abdominal pré-operatória em cães e gatos com suspeita de tumores abdominais DAA Garcia, TR Froes, SD Guérios Ciência Rural 42 (1), 105-111 , 2012 2012 Citations: 16