PEGylated Silk Fibroin Nanoparticles for Oral Antibiotic Delivery: Insights into Drug-Carrier Interactions and Process Greenness Duy Toan Pham, Thi Truc Dao Le, Ngoc Yen Nguyen, Chong Kim Thien Duc, Nguyen Trong Tuan, Huynh Vu Thanh Luong, Quyen Thi Bich Tran, Manh Quan Nguyen, Bui Thi Phuong Thuy ACS Omega, 2025 Insights into antibiotic–carrier interactions in the silk-fibroin-based nanoparticles and considerations on the formulation process greenness are limited. Hence, this work developed and characterized polyethylene glycol-functionalized silk fibroin nanoparticles for oral delivery of cefotaxime (PEG/SFPs-CTX), with a focus on the in silico simulations and process greenness. The particles were formulated by the two methods of co-condensation and adsorption. Dependent on the processes, the particles possessed spherical shape, smooth/rough surfaces, nanosize (170–650 nm), negative charge (−23 to −30 mV), and drug entrapment efficiency of ∼50%. The system rapidly absorbed the drug within 30 min, followed second-order kinetics, and significantly controlled the drug release rate in the simulated gastrointestinal system, which could bypass the stomach acidic pH. Interestingly, in silico docking revealed that CTX mostly bound strongly and noncovalently with fibroin, particularly at glutamic acid, via hydrogen bonds, van der Waals forces, and π–π interactions, while PEG enhanced the stability of the system. Molecular dynamics simulations confirmed the complex’s stability under physiological conditions. Lastly, life cycle assessment analysis showed that both formulation methods were environmental friendly, with limited impacts on the ecosystem, and the adsorption method was “greener” than the co-condensation method.
Naturally derived hydrogels for wound healing Duy Toan Pham, Ngo Thi Ngoc Thuy, Nguyen Thi Phuong Thao, Le Thi Nhi, Bui Thi Phuong Thuy Therapeutic Delivery, 2025 Natural hydrogels have garnered increasing attention due to their natural origins and beneficial roles in wound healing. Hydrogel water-retaining capacity and excellent biocompatibility create an ideal moist environment for wound healing, thereby enhancing cell proliferation and tissue regeneration. For this reason, naturally derived hydrogels formulated from biomaterials such as chitosan, alginate, gelatin, and fibroin are highly promising due to their biodegradability and low immunogenic responses. Recent integrated approaches to utilizing new technologies with bioactive agents have significantly improved the mechanical properties of hydrogels and the controlled release and delivery of active compounds, thereby increasing the efficiency of the treatment processes. Herein, this review highlights the advantages and the challenges of natural hydrogels in wound healing, focusing on their mechanical strength, controlled degradation rates, safety and efficiency validation, and the potential for incorporating advanced technologies such as tissue engineering and gene therapy for utilization in personalized medicine.
Development of pH-responsive Eudragit S100-functionalized silk fibroin nanoparticles as a prospective drug delivery system Duy Toan Pham, Doan Xuan Tien Nguyen, Ngoc Yen Nguyen, Thi Truc Linh Nguyen, Thanh Q. C. Nguyen, Anh Vo Thi Tu, Ngoc Huyen Nguyen, Bui Thi Phuong Thuy Plos One, 2024 Silk fibroin nanoparticles (FNP) have been increasingly investigated in biomedical fields due to their biocompatibility and biodegradability properties. To widen the FNP versatility and applications, and to control the drug release from the FNP, this study developed the Eudragit S100-functionalized FNP (ES100-FNP) as a pH-responsive drug delivery system, by two distinct methods of co-condensation and adsorption, employing the zwitterionic furosemide as a model drug. The particles were characterized by sizes and zeta potentials (DLS method), morphology (electron microscopy), drug entrapment efficiency and release profiles (UV-Vis spectroscopy), and chemical structures (FT-IR, XRD, and DSC). The ES100-FNP possessed nano-sizes of ∼200–350 nm, zeta potentials of ∼ -20 mV, silk-II structures, enhanced thermo-stability, non-cytotoxic to the erythrocytes, and drug entrapment efficiencies of 30%-60%, dependent on the formulation processes. Interestingly, the co-condensation method yielded the smooth spherical particles, whereas the adsorption method resulted in durian-shaped ones due to furosemide re-crystallization. The ES100-FNP adsorbed furosemide via physical adsorption, followed Langmuir model and pseudo-second-order kinetics. In the simulated oral condition, the particles could protect the drug in the stomach (pH 1.2), and gradually released the drug in the intestine (pH 6.8). Remarkably, in different pH conditions of 6.8, 9.5, and 12, the ES100-FNP could control the furosemide release rates depending on the formulation methods. The ES100-FNP made by the co-condensation method was mainly controlled by the swelling and corrosion process of ES100, and followed the Korsmeyer-Peppas non-Fickian transport mechanism. Whereas, the ES100-FNP made by the adsorption method showed constant release rates, followed the zero-order kinetics, due to the gradual furosemide dissolution in the media. Conclusively, the ES100-FNP demonstrated high versatility as a pH-responsive drug delivery system for biomedical applications.
Insight QSDAR models for prediction of anticancer activity on Hela cell line of new flavonoid isolating from rhizome Zingiber zerumbet SM in Viet Nam Indian Journal of Chemistry Section B Organic and Medicinal Chemistry, 2021
Investigation into SARS-CoV-2 Resistance of Compounds in Garlic Essential Oil Bui Thi Phuong Thuy, Tran Thi Ai My, Nguyen Thi Thanh Hai, Le Trung Hieu, Tran Thai Hoa, Huynh Thi Phuong Loan, Nguyen Thanh Triet, Tran Thi Van Anh, Phan Tu Quy, Pham Van Tat, Nguyen Van Hue, Duong Tuan Quang, Nguyen Tien Trung, Vo Thanh Tung, Lam K. Huynh, Nguyen Thi Ai Nhung ACS Omega, 2020
Rainwater for drinking in Vietnam: Barriers and strategies Bui Thi Thuy, Anh Dung Dao, Mooyong Han, Duc Canh Nguyen, Viet Anh Nguyen, Hyunju Park, Pham Dang Manh Hong Luan, Nguyen Thi Thanh Duyen, Hong Quan Nguyen Journal of Water Supply Research and Technology Aqua, 2019