Sequential chemical and enzymatic pretreatment of palm empty fruit bunches for Candida pelliculosa bioethanol production Tania Sila Campioni, Carlos Ricardo Soccol, Nelson Libardi Junior, Cristine Rodrigues, Adenise Lorenci Woiciechowski, Luiz Alberto Junior Letti, Luciana Porto de Souza Vandenberghe Biotechnology and Applied Biochemistry, 2020 Second‐generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical treatments indeed attacked the cellulose fibers and removed the silica from surface pores. The optimization of enzymatic hydrolysis of EFB's cellulosic fraction was performed with 0.5%–4% v/v of Cellic® CTec2/Novozymes, different EFB concentrations (5%–15%, w/v), and hydrolysis time (6–72 H). Optimization essays were carried out in Erlenmeyer flasks and also in a 1 L stirred tank reactor. After enzymatic hydrolysis, a hydrolysate with 66 g/L of glucose was achieved with 2.2% (v/v) Cellic® CTec2, 15% (m/v) acid/alkaline pretreated EFB after 39 H of hydrolysis. A gain of 11.2% was then obtained in the 1 L stirred tank promoted by the agitation (72.2 g/L glucose). The hydrolysate was employed in bioethanol production by a new isolate Candida pelliculosa CCT 7734 in a separate hydrolysis and fermentation process reaching 16.6 and 23.0 g/L of bioethanol through batch and fed‐batch operation, respectively. An integrated biorefinery process was developed for EFB processing chain.
Fibrolytic enzymes improving in vitro rumen degradability of tropical forages Gabriel Zanuto Sakita, Thiago Francisco Ventoso Bompadre, Dhanasekaran Dineshkumar, Paulo de Mello Tavares Lima, Adibe Luiz Abdalla Filho, Tania Sila Campioni, Pedro Oliva Neto, Herman Bremer Neto, Helder Louvandini, Adibe Luiz Abdalla Journal of Animal Physiology and Animal Nutrition, 2020 Abstract During specific times of the year, especially dry seasons, tropical forages typically have poor nutritional value due to high contents of neutral and acid detergent fibres, and low crude protein in their composition, which may reduce productivity of ruminant livestock production and lead to increased enteric methane (CH 4 ) emissions per unit of generated product in forage‐bases systems. In order to increase fibre degradability and the efficiency of energy utilisation from low‐quality forages, exogenous fibrolytic enzymes have been studied. In this assay, we evaluated the effects of increasing dose levels of fibrolytic enzymes extract (FEE) produced by Trichoderma reesei on in vitro rumen organic matter degradability, fermentation parameters, total gas and CH 4 production of tropical forages. Forage samples were analysed for their bromatological composition, and enzyme activity from FEE was performed for xylanase and endoglucanase. The i n vitro gas production technique was used in a 5 × 3 factorial arrangement with five FEE dose levels (0, 5, 50, 500 and 5,000 µl) and three substrates ( Cynodon spp., Panicum maximum and Cenchrus ciliaris L.). The highest dose level of FEE increased degradability, total gas and CH 4 production in all substrates ( p < .05). Butyrate concentration also increased while acetate:propionate ratio and pH decreased with the addition of FEE ( p < .05). These results indicated that the use of fibrolytic enzymes can be a reliable strategy to improve degradability of low‐quality forages, contributing to the sustainability and intensification of livestock production in tropical countries.
Lactic acid production from submerged fermentation of broken rice using undefined mixed culture Luiza Varela Nunes, Fabiane Fernanda de Barros Correa, Pedro de Oliva Neto, Cassia Roberta Malacrida Mayer, Bruna Escaramboni, Tania Sila Campioni, Natan Roberto de Barros, Rondinelli Donizetti Herculano, Eutimio Gustavo Fernández Núñez World Journal of Microbiology and Biotechnology, 2017
