Dr Dolly Sharma

PATENTS FILED: 3 COMPLETE PATENTS FILED AND PUBLISHED (Under examination)
• Title: “Fluorine modified phenoxy benzene based small molecules inhibitors of lactate dehydrogenase (LDH)and method”.
Inventor: Dolly Sharma, Reshma Rani, Vinit Kumar, Application number: 202011052759
• Title: “A process of synthesis of Guanidine-based biorelevant ligands (GLBS) and composition
Inventor: Dolly Sharma, Reshma Rani, Vinit Kumar, Mamta Singh , Application number: 202111044830
• Title: “Venetoclax as a potential repurposed drug in targeting breast cancer and breast cancer stem cells”.
Inventors: Dolly Sharma, Yamini Pathak, Vishwas Tripathi, Amaresh Mishra, Bhaswati Banerjee Application number: 202411059892
PUBLICATIONS:
1. Sharma, Dolly, Yadav, Anubha; Saini, Sachin; Singh, Mamta;; Mattoo, Shria; Kashyap, Akanksha; Pore, Subrata; Prasad, Chandra Prakash; Rajput, Chatrasal; Rani, Reshma; Kumar, Vinit. Synthesis and Evaluation of Novel Fluorinated Quinoline Derivatives in 2D and 3D Model

EDUCATION

• Ph.D. in Biotechnology, Degree Awarded 2024, Amity University Noida (CGPA: 7.8)
• 5-Year Integrated Dual Degree Program B. Tech + M. Tech in Biotechnology (Jul 2012 - May 2017): 6.83 CGPA equivalent to 68.3%
Gautam Buddha University, Greater Noida Jul 2012 - May 2017
• 12th in 2011, CBSE Board, English Medium: 82%
Ram-Eesh International School, Greater Noida, U.P.
• 10th in 2009, CBSE Board, English Medium: 81%
Ram-Eesh International School, Greater Noida, U.P.

RESEARCH, TEACHING, or OTHER INTERESTS

Biotechnology, Cancer Research, Organic Chemistry, Multidisciplinary

Scopus Publications

COMPARATIVE ANALYSIS OF NON-VIRAL GENE DELIVERY SYSTEMS AND THEIR IMPACT ON CELLULAR UPTAKE AND GENE EXPRESSION EFFICIENCY
R Latha, Rajeswari S, Baskaran Kuppusamy, Sudhakar K, Ankit Sachdeva, et al.
Genetics and Molecular Research, 2026
Non-viral gene delivery systems have become very popular because they are safer and more universal than viral vectors and have various benefits, including decreased immunogenicity, enhanced scalability, and versatile structure. Their extensive use in clinical practice is, however, restricted by poor efficiency in transfection and numerous intracellular obstacles. This paper provides a comparative study carried out in a systematic manner of 3 of the most well-known non-viral gene delivery platforms, such as lipid-based nanoparticles, polymeric carriers, dendrimers, and hybrid nanomaterials, regarding their ability to influence cellular uptake and cellular efficiency of gene delivery. The systematic analysis framework was used to analyze important parameters of physicochemical qualities in terms of particle size, surface charge, and compositional architecture, and their relation to biological performance. Endocytosis, endosomal escape, cytoplasmic transport, and nuclear import are some of the critical steps in delivery that were studied to find out factors that control the transgene expression rate. The results show that lipid-based and hybrid systems are better uptake and expression systems because of the increased association with membrane and an enhanced endosomal escape system whereas polymeric systems show constraints that is related to cytotoxicity and intracellular degradation. Moreover, profound surface engineering approaches like PEGylation and ligand functionalization are important to increase the stability of delivery and specificity in targets. In general, this research offers mechanical explanations and design principles of the optimization of non-viral gene delivery systems, to obtain a better therapeutic effect.
Exploring gedunin’s potential in cancer therapy: current advances and future perspectives
Deepa Bisht, Dolly Sharma, Shaily Chauhan, Shikha Singh, Puniethaa Prabhu, Piyush Kumar Gupta
Proceedings of the Indian National Science Academy, 2025
Synthesis and Evaluation of Novel Fluorinated Quinoline Derivatives in 2D and 3D Models of Triple-Negative Breast Cancer
Anubha Yadav, Sachin Saini, Mamta Singh, Dolly Sharma, Shria Mattoo, Akanksha Kashyap, Subrata Kumar Pore, Chandra Prakash Prasad, Chatrasal Singh Rajput, Reshma Rani, Vinit Kumar
ACS Omega, 2025
High Resolution Image Download MS PowerPoint Slide Triple-negative breast cancer (TNBC) is a particularly aggressive subtype of breast cancer that is more likely to metastasize and become resistant with a worse prognosis. Herein, we have synthesized novel fluorinated quinoline analogues and investigated their anticancer properties against breast cancer cell lines and cancer cell spheroids. Compounds 6a, 6b, 6d, and 6f demonstrated potent anticancer activity with IC 50 values of 2.5–5 μM against TNBC cells (MDA-MB-468), while remaining nontoxic to nontumorigenic breast cells (MCF-10A). The hydrolyzed analogues 7a–f were not active against TNBC cells, implying that the ester group together with fluorine substitution on the quinoline ring is critical for their anticancer activity. Compounds 6a, 6b, and 6f significantly reduced the clonogenic capacity of TNBC cells and induced reactive oxygen species (ROS). Notably, compounds 6b and 6f considerably decreased TNBC cells (MDA-MB-468 and 4T1) 3D spheroids volume. The present research opens new avenues for developing novel therapeutics for breast cancers.
Genomic Approaches for Alfalfa Breeding: Advances and Future Prospects
Urmila Devi, Jatinder Paul Kaur, Monika Bansal, Dolly Sharma, Shabir Hussain Wani, Arafat Abdel Hamed Abdel Latef
Accelerated Plant Breeding Volume 5 Forage Crops, 2025
Advancing pandemic response: The impact of nano diagnostics on COVID-19 and other infectious diseases
M.S. Hussain, Amaresh Mishra, Neelima Varshney, Km Shivangi, Dolly Sharma, Bhaswati Banerjee, Manju Yadav, Vishwas Tripathi
Nanotechnology and Antivirus Nanomaterials for Combating Covid 19 Strategies Against Sars Cov 2 Variants in the Post Pandemic Era, 2025
Plant-derived polyphenols in modulating the immune response against COVID-19
Amaresh Mishra, Km Shivangi, Manju Yadav, Dolly Sharma, Jyoti Upadhyay, et al.
Anti Sars Cov 2 Activity of Flavonoids, 2024
During the COVID-19 pandemic, it is crucial to prioritize the discovery of truly effective treatments. Inflammation and immune dysregulation contribute to the pathogenesis of COVID-19, with severe outcomes linked to cytokine storms and uncontrolled inflammatory responses. Polyphenols found in plant-based foods are known as dietary polyphenols and they possess anti-inflammatory and immunomodulatory properties. In this chapter, we review the immunomodulatory effects of dietary polyphenols and evaluate their potential in managing COVID-19. We discuss their ability to suppress inflammatory pathways, reduce oxidative stress, and regulate immune cell functions. Additionally, we investigate the available evidence regarding the antiviral properties of polyphenols and their potential implications in the battle against SARS-CoV-2. Further, we integrate findings from cancer biology, focusing on the relevance of targeting inflammation and immune dysregulation in breast cancer stem cells with dietary polyphenols related to COVID-19. This book chapter examines the efficacy of nutritional polyphenols in mitigating inflammation and regulating the immune response to COVID-19. We aim to provide insights into the multifaceted benefits of dietary polyphenols, emphasizing their potential as adjunctive therapies for COVID-19 and their relevance for future drug development and therapeutic strategies.
Design and Synthesis of Thiazole Scaffold-Based Small Molecules as Anticancer Agents Targeting the Human Lactate Dehydrogenase A Enzyme
Dolly Sharma, Mamta Singh, Jayadev Joshi, Manoj Garg, Vidhi Chaudhary, et al.
ACS Omega, 2023
High Resolution Image Download MS PowerPoint Slide A new series of thiazole central scaffold-based small molecules of h LDHA inhibitors were designed using an in silico approach. Molecular docking analysis of designed molecules with h LDHA (PDB ID: 1I10 ) demonstrates that Ala 29, Val 30, Arg 98, Gln 99, Gly 96, and Thr 94 possessed strong interaction with the compounds. Compounds 8a, 8b, and 8d showed good binding affinity (−8.1 to −8.8 kcal/mol), whereas an additional interaction of NO 2 at the ortho position in compounds 8c with Gln 99 through hydrogen bonding enhanced the affinity to −9.8 kcal/mol. Selected high-scored compounds were synthesized and screened for h LDHA inhibitory activities and in vitro anticancer activity in six cancer cell lines. Biochemical enzyme inhibition assays showed the highest h LDHA inhibitory activity observed with compounds 8b, 8c, and 8l . Compounds 8b, 8c, 8j, 8l, and 8m depicted significant anticancer activities, exhibiting IC 50 values in the range of 1.65–8.60 μM in HeLa and SiHa cervical cancer cell lines. Compounds 8j and 8m exhibited notable anticancer activity with IC 50 values of 7.90 and 5.15 μM, respectively, in liver cancer cells (HepG2). Interestingly, compounds 8j and 8m did not induce noticeable toxicity in the human embryonic kidney cells (HEK293). In silico absorption, distribution, metabolism, and excretion profiling demonstrates that the compounds possess drug-likeness, and results may pave the way for the development of novel thiazole-based biologically active small molecules for therapeutics.
Targeting monocarboxylate transporters (MCTs) in cancer: How close are we to the clinics?
Mamta Singh, Julieta Afonso, Dolly Sharma, Rajat Gupta, Vivek Kumar, et al.
Seminars in Cancer Biology, 2023
Targeting Virus-Induced Reprogrammed Cell Metabolism via Glycolytic Inhibitors: An Effective Therapeutic Approach Against SARS-CoV-2
Vinit Kumar, Reshma Rani, Dolly Sharma, Mamta Singh, Rajat Gupta, et al.
Mini Reviews in Medicinal Chemistry, 2023
Reprogrammed cell metabolism has been observed in a wide range of virally infected cells. Viruses do not have their metabolism; they rely on the cellular metabolism of the host to ensure the energy and macromolecules requirement for replication. Like other viruses, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) does not own its metabolism, but virus infected cells adopt aberrant cell metabolism. Infected viral use the energy and macromolecules to make their own copies; to do so, they need to increase the rate of metabolism to ensure the requirement of macromolecules. In contrast, the cellular metabolism of noninfected cells is more plastic than infected cells. Therefore, it is essential to examine the virus infection in the context of metabolic alterations of host cells. A novel therapeutic approach is urgently required to treat highly infectious COVID-19 disease and its pathogenesis. Interference of glucose metabolism might be a promising strategy to determine COVID-19 treatment options. Based on the recent research, this mini-review aims to understand the impact of reprogrammed cell metabolism in COVID-19 pathogenesis and explores the potential of targeting metabolic pathways with small molecules as a new strategy for the development of a novel drug to treat COVID-19 disease. This type of research line provides new hope in the development of antiviral drugs by targeting hijacked cell metabolism in case of viral diseases and also in COVID-19.
Role of LDH in tumor glycolysis: Regulation of LDHA by small molecules for cancer therapeutics
Dolly Sharma, Mamta Singh, Reshma Rani
Seminars in Cancer Biology, 2022
Current understanding of biological interactions and processing of DNA origami nanostructures: Role of machine learning and implications in drug delivery
Mamta Singh, Dolly Sharma, Manoj Garg, Atul Kumar, Ankur Baliyan, et al.
Biotechnology Advances, 2022
Intervention on lactate in cancer: A promising approach for the development of cancer therapeutics
Dolly Sharma, Mamta Singh, Rajat Gupta, Vivek Kumar, Vinit Kumar, et al.
Advances in Cancer Biology Metastasis, 2022
Design, synthesis, in silico ADME/T profiling of pseudo-pyrimidine derivatives and anticancer activity evaluation
Dolly Sharma, Vinit Kumar, Reshma Rani
Research Journal of Chemistry and Environment, 2022