Phenotyping and Genotyping Evaluation of E. coli Produces Carbapenemase Isolated from Cancer Patients in Al-Basrah, Iraq A. Ali Albadery, S. Shakir Mahdi Al-Amara, A. Abd-Al-Ridha Al-Abdullah Archives of Razi Institute, 2023 One of the most important nosocomial organisms that cause urinary tract infections (UTIs) in cancer patients is Escherichia coli. A significant cause of concern in managing UTIs is the development of carbapenem-resistant bacteria. Escherichia coli with carbapenem resistance has become a more serious problem, particularly in Iraq. In this regard, the present study aimed to estimate the prevalence of carbapenem-resistant E. coli in Al-Basrah, Iraq. Conventional tests and the Vitek®2 system were used to identify the isolates and determine the susceptibility of E.coli isolates to antimicrobials. In addition, E.coli isolates were tested by mCIM and eCIM methods. Moreover, the major carbapenemase genes, including blaSPM, blaIMP, blaVIM, and blaKPC were detected by polymerase chain reaction. In total, 120 urine samples were collected from cancer patients who were suspected of having urinary tract infections at Basrah Center of Oncology Al-Sader Teaching Hospital, Basrah, Iraq. Identification of bacterial growth by using biochemical tests revealed different bacterial species. The most frequent bacteria were E. coli (n=22, 53.65%) isolates. The results showed that 13 (59.09%) and 11 (50%) out of 22 E. coli isolates were positive for the production of carbapenemase, based on the eCIM and sCIM, respectively. The majority of E.coli in this study possessed the blaVIM gene (n=13, 59.1%), followed by the blaKPC gene (n=5, 22.73%), blaIMP gene (n=5, 22.73%), and blaSPM gene (n=4, 18.18%). There is a spread of more than one type of carbapenemase among the E. coli isolates collected from UTI cancer patients in Basrah Hospital. The E. coli identified in the current study had a strong capacity to produce carbapenemase enzymes against the four generations of antibiotics, including imipenem and meropenem antibiotics.
Detection of Extended-Spectrum ß-Lactamases (ESBLs), TEM, SHV and CTX-M genes among Staphylococcus haemolyticus isolates from cesarean section infections Ginekologia I Poloznictwo, 2023
Constitutive and Inducible Clindamycin Resistance Frequencies among Staphylococcus sp. Coagulase Negative Isolates in Al-Basrah Governorate, Iraq Reports of Biochemistry and Molecular Biology, 2022
Molecular detection of new bacillus strains from soil samples of free grazing areas in Basrah Province, Southern Iraq Murtakab Y Al-Hejjaj, Saad S Al-Amara, Yessar A Dawood, Shaymaa Jabbar Raisan, Hassan M AlTameemi Annals of Tropical Medicine and Public Health, 2020 Bacterial contamination is an important indicator for soil quality that could be related to the plant’s suitability for animals ‘grazing and ultimately human public health. Consequently, the microbial characteristics of soil could be linked to animals’ products quality, such as milk and meat. Bacillus species is one of the most distributed soil contaminating bacteria and is linked to animals’ products quality. Three different grazing areas (Abo Al-Kasib, Al-Seba, and Al-Fawdistricts) in Basrah province were selected to determine the bacterial (Bacillus)distribution in soil. Samples were randomly collected from each field of the study. Based on the bacterial cell shape and staining (microscopy), colony morphology, biochemical tests, and the 16S rRNA genes amplification results, the bacterial isolates were identified to be from the genus Bacillus, of which Bacillus cereus was dominant in all samples. Five new isolates were identified based on 16S rRNA nucleotide sequences. Phylogenic analysis showed a close relation between three isolates to Bacillus cereuswith high 16S rRNA gene sequences similarity 99% (SAMU1), 99% (SAMU2) and 98.9% (SAMU3). Furthermore, two isolates showed sequences similarity to Bacillus paramycoides (99%, SAMU4) and Bacillus safensis (99%, SAMU5). All the above isolates were registered in the NCBI centre under the following names Bacillus cereus strain SAMU1, Bacillus cereus strain SAMU2,Bacillus cereus strain SAMU3,Bacillus paramycoidess strain SAMU4 and Bacillus safensis strain SAMU5 and were assigned the following accession numbers MK418732, MK490900, MK490901, MK490902,and MK490903respectively.In addition, some isolates were found to be 100% similar to the already identified Bacillus strains such as Bacillus safensis, B. pummilus, B. paramycoides and B. safensis. The current study investigated and mapped the distribution of Bacillus Spp in animals grazing areas that had been subjected to salinity increment.
