David Sanchez Porras

Scopus Publications

Comparative Assessment of Microbial Colonization and Tissue Reaction Among Three Suture Materials: A Randomized Controlled Trial
José Manuel Alarcón Cordovilla, María Victoria Olmedo-Gaya, María Teresa Arias-Moliz, Adela Baca García, David Sánchez-Porras, María Pilar Quesada-García, María Nuria Romero-Olid
Antibiotics, 2025
Background: The aim of this study was to evaluate and compare the bacterial colonization, cytotoxicity, immune response, and clinical parameters of three different suture materials: multifilament silk (Silk®), monofilament nylon (Daclon®), and expanded polytetrafluoroethylene monofilament (PTFE®), in surgical extractions of impacted mandibular third molars. Methods: This randomized controlled clinical trial was conducted on twenty-one patients requiring surgical extraction of an impacted third mandibular molar. A bayonet-shaped flap was sutured using all three materials in each patient. Bacterial cell counting and qPCR were assessed for microbiological analysis. In vitro cytotoxicity was studied with the metabolic activity WST-1 assay. Inflammatory response was evaluated through histological analysis. Clinical parameters—healing, handling, slack, pain, swelling and trimus—were recorded. Statistical significance was set at p ≤ 0.05. Results: Monofilament sutures accumulated fewer bacteria and DNA copies than Silk® (p < 0.05). The WST-1 assay revealed non-cytotoxic effects. Silk® presented an immune response with lymphocyte-like cells. The highest values of pain and inflammation were reached at 48 h, with a significant correlation between them (p < 0.05). Silk and nylon were more manageable than PTFE (p < 0.001), and nylon had less slack (p < 0.001). Conclusions: Silk showed the poorest microbiological and histological performance, with higher levels of bacterial colonization and a more pronounced inflammatory response compared to the other types of suture. Clinically, it offered better handling than PTFE (PTFE®), comparable to nylon (Daclon®), but it exhibited greater slack, which could prove less favorable for wound stability. None of the sutures showed in vitro cytotoxicity. Monofilament sutures, particularly nylon (Daclon®), showed better outcomes, acceptable handling, less bacterial colonization, and a milder inflammatory response.
Generation of a Bioengineered Substitute of the Human Sclero-Corneal Limbus Using a Novel Decellularization Method
Paula Ávila-Fernández, David Sánchez-Porras, Miguel Etayo-Escanilla, Carmen González-Gallardo, Miguel Alaminos, Jesús Chato-Astrain, Fernando Campos, Óscar Darío García-García
Pharmaceutics, 2025
Background: Severe dysfunction of the human limbus associated with limbal stem cell deficiency is a therapeutic challenge, especially when a structural alteration of the limbal niche is associated. Methods: We have evaluated seven decellularization protocols applied to 20 human sclero-corneal limbus, based on the use of SDS (protocol P1), SDS + NaCl (P2), SDS + triton X-100 + SDC + enzymatic treatment (P3), SDS + triton X-100 + SDC + enzymatic treatment + trypsin (P4), sulfobetains + DNAse (P5), sulfobetains + SDC + DNAse (P6) and SDC + DNAse (P7). The decellularization efficiency of each protocol, biocompatibility and safety, as well as their capability to support cell attachment and differentiation, were evaluated. Results: Results showed that the use of protocols P1 to P4, based on strong ionic detergents such as SDS, was not efficient for decellularizing the human limbus. Conversely, protocols P5, P6 and P7 removed more than 95% of DNA while preserving 60–100% of the extracellular matrix components. These protocols were biocompatible, as macrophages cultured with decellularized scaffolds were viable and differentiated to the pro-regenerative M2 phenotype (CD163/CD86 ratio > 2) without inducing a significant increase in reactive oxygen species (ROS). Protocols P6 and P7 supported cell attachment, survival and differentiation of corneal epithelial cells and four types of mesenchymal stem cells cultured on the surface of these scaffolds. Cellularized limbi showed positive expression of several limbal cell markers, especially in scaffolds decellularized with protocol P6. Conclusions: These results support the use of protocol P6 for the generation of human limbal substitutes by tissue engineering using decellularized human limbi. Future studies should determine the clinical potential of the regenerative biomaterial generated in patients with structural limbal damage, particularly in patients with chemical burns and aniridia, where conventional stem cell therapies fail.
Novel genipin-crosslinked acellular biogenic conduits for tissue engineering applications
Óscar Darío García-García, Sandra Escalante-Quirós, Claudia Llinares-Monllor, Paula Ávila-Fernández, David Sánchez-Porras, Miguel Etayo-Escanilla, Fernando Campos, Jesús Chato-Astrain, Víctor Carriel
Biomedicine and Pharmacotherapy, 2025
BACKGROUND: Collagen-based conduits have been generated in-vivo stimulating a fibrotic response through the implantation of a non-resorbable material in animal models, creating biogenic substitutes. However, they often exhibit clinical limitations due to prolonged generation times, exclusive autologous use and insufficient mechanical strength. Consequently, decellularization and cross-linking could solve the aforementioned drawbacks, providing a non-immunogenic and ready-to-use natural substitute with enhanced biomechanical properties. Nevertheless, these processes may alter microarchitecture and biocompatibility. Hence, this is the first study to characterize ex-vivo the biogenic conduits of 1-and 2-months maturation time which were subjected to decellularization and genipin (GP) cross-linking procedures performing histological, structural, biomechanical, biocompatibility, and immunological analyses to identify the most suitable option for peripheral nerve regeneration. RESULTS: Histological examination indicated consistent uniformity of the biogenic conduits at both timepoints post-implantation, maintaining their overall structural integrity and collagen pattern following decellularization and GP crosslinking treatments. Furthermore, no evidence of nuclear debris was observed in the decellularized groups at either stage of maturation, confirming the decellularization protocol's efficiency. The substitutes with longer maturation time presented a generally higher preservation of ECM key components. In addition, the GP crosslinking significantly increased the resistance values of decellularized biogenic conduits, without drastically affecting the ex-vivo cell biocompatibility nor macrophage polarization rate phenotype. CONCLUSIONS: These findings indicate the suitability of our decellularization protocol for biogenic conduits, and subsequent crosslinking with GP improves their biomechanical properties without altering their biocompatibility or immunological profile, suggesting their potential as a ready-to-use tubular substitute for nerve and other tissue engineering applications.
The impact of COVID-19 pre-university education on first-grade medical students. A performance study of students of a Department of Histology
José Manuel García, David Sánchez‐Porras, Miguel Etayo‐Escanilla, Paula Ávila‐Fernández, Olimpia Ortiz‐Arrabal, Miguel‐Ángel Martín‐Piedra, Fernando Campos, Óscar‐Darío García‐García, Jesús Chato‐Astrain, Miguel Alaminos
Anatomical Sciences Education, 2025
The recent coronavirus disease (COVID‐19) forced pre‐university professionals to modify the educational system. This work aimed to determine the effects of pandemic situation on students' access to medical studies by comparing the performance of medical students. We evaluated the performance of students enrolled in a subject taught in the first semester of the medical curriculum in two pre‐pandemic academic years (PRE), two post‐pandemic years (POST), and an intermediate year (INT) using the results of a final multiple‐choice exam. Consistency analysis among periods was performed using the Cronbach alpha coefficient (α), the difficulty index with random effects correction (DI), and the point‐biserial correlation index (PB). The five exams were homogeneous and had similar α, DI, and PB difficultness. Performance significantly decreased in POST students compared with PRE students, with a correlation between performance and the academic years (PRE‐POST). A significant decrease in the percentage of correct answers was detected in the academic years, with POST students showing lower results than PRE students, but not in the percentage of questions answered incorrectly. Significantly higher percentages of unanswered questions were found among POST students. These results confirm the negative impact of the POST pre‐university educational system on the performance of students accessing medical school and suggest that POST students could have a higher degree of uncertainty. Specific education programs should be implemented during the first years of the medical curriculum to tailor this effect and increase students' self‐confidence and knowledge, which may be associated with confidence.
A gene expression and a histostructural analysis of the palmar fascia of patients affected by Dupuytren’s disease
Adolfo Galán Novella, Olimpia Ortiz-Arrabal, David Sánchez-Porras, Fabiola Bermejo-Casares, Enrique Guerado, Miguel Alaminos
Connective Tissue Research, 2025
PURPOSE Dupuytren's disease (DD) is a condition affecting the palmar fascia that may reduce the mobility of several fingers. Despite its clinical relevance, the genetic and structural mechanisms associated with this disease are still not well understood. In this work, we have carried out a genome-wide gene expression analysis to identify relevant genes associated with DD. METHODS A genome-wide gene expression analysis was carried out using next generation sequencing (NGS) followed by a histological, histochemical and immunohistochemical analysis of some major components of the palmar fascia in 26 DD patients and 17 control subjects without the disease (CTR). RESULTS We found 237 genes or sequences differentially expressed between DD and CTR, with those genes corresponding to several gene pathways and functions related to contractility, development and morphogenesis, differentiation, extracellular matrix (ECM), migration and ossification. In turn, the histological analysis confirmed that DD tissues showed a disorganized ECM, with nonaligned fibers, and abundant cells were found scattered along the whole tissue. CTR showed significantly higher amounts of proteoglycans revealed by alcian blue, along with versican, keratan-sulfate, myoglobin, tropomyosin 3, filamin C and titin, whereas DD showed significantly enriched in collagen fibers, especially collagens type-I and V, MMP-14, S-100, tubulin-beta, SMA and tenascin C, with disorganization of the elastic fibers. CONCLUSIONS In general, these results confirm that a significant alteration of the tissue organization, extracellular matrix and structure is related to DD. These results could contribute to the future development of diagnostic and treatment strategies for this disease.
Tissue Engineering for Oral Mucosa Biofabrication: A Systematic Review and Meta-Analysis
Miguel A. Martin-Piedra, Manuel Albendin-Moreno, Adriana Olivares-Abril, Sara V.J. Paez-Yepes, Mario Rivera-Izquierdo, et al.
Tissue Engineering Part B Reviews, 2025
Tissue engineering of oral mucosa has emerged as a promising alternative for reconstructing oral lesions. This systematic review and meta-analysis evaluated advancements in the biofabrication of artificial oral mucosa, focusing on its components, methods, and outcomes. A total of 57 studies were included, primarily preclinical in vitro research. The predominant cell sources were primary oral keratinocytes and fibroblasts, with collagen being the most utilized biomaterial. The immersion and air technique was the main biofabrication method. The meta-analysis revealed an average epithelial thickness of 73.18 μm and a maturation score of 5.13/6. In vivo studies indicated a trend toward greater epithelial stratification compared with in vitro studies. The presence of cellularized stroma, decellularized scaffolds, and custom growth factors correlated with increased epithelial thickness although without statistically significant differences. This study provides a comprehensive overview of the current state of tissue-engineered oral mucosa, highlighting its clinical potential. Impact Statement This systematic review and meta-analysis provides the most comprehensive synthesis to date of biofabrication strategies for tissue-engineered oral mucosa (TEOM). By critically evaluating 57 studies, it evaluates key design variables—such as biomaterials, cell sources, and culture techniques—and their influence on epithelial thickness and maturation. The findings highlight the translational relevance of TEOM for future clinical applications.
Generation and ex vivo characterization of a full-thickness substitute of the human urethra by tissue engineering
David Sánchez‐Porras, Miguel Etayo‐Escanilla, José‐Andrés Moreno‐Delgado, María del Mar Lozano‐Martí, Fabiola Bermejo‐Casares, Miguel Alaminos, Jesús Chato‐Astrain, Fernando Campos, M. Carmen Sánchez‐Quevedo, Ricardo Fernández‐Valadés
Bioengineering and Translational Medicine, 2025
Tissue engineering may offer efficient alternatives for the surgical repair of severe conditions affecting the human urethra. However, development of tubular full‐thickness substitutes is challenging. In this work, we have generated and evaluated ex vivo a novel full‐thickness human urethra substitute (FHUS) containing its three main layers: the urethral mucosa (UM), the spongy layer (SP), and the tunica albuginea (AL). Results first showed that the generation of a FHUS significantly improved the biomechanical properties of this artificial tissue as compared to the individual layers, although the resistance of the native urethra was not reached. At the structural level, we found that FHUS shared important histological similarities with the native urethra. Analysis of the individual layers showed that UM had a stratified epithelium that expressed several epithelial markers, including cytokeratins CK7 and CK14, uroplakin 1b, and the intercellular junction proteins desmoplakin, tight junction protein 1, and claudin. At the stromal level, UM tended to increase the presence of collagen fibers and versican with time. The SP layer displayed abundant CD31 and CD34‐positive blood vessels, but small amounts of collagen and proteoglycans. The AL layer showed scattered smooth muscle cells expressing α‐smooth muscle actin, smoothelin, and desmin cell markers, and contained low amounts of collagen and proteoglycans. Analysis of the basement membrane components collagen IV and laminin revealed their progressive development with time, especially collagen IV. These results confirm the possibility of developing a partially biomimetic full‐thickness substitute of human urethra that might have potential clinical usefulness for the clinical repair of severe urethral lesions.
Phase I-IIa clinical trial to evaluate the safety, feasibility and efficacy of the use of a palate mucosa generated by tissue engineering for the treatment of children with cleft palate: the BIOCLEFT study protocol
Antonio España-López, Ricardo Fernández-Valadés, Elisa Cubiles, Ingrid Garzón, Miguel Angel Martin-Piedra, Víctor Carriel, Fernando Campos, Adoración Martínez-Plaza, Daniel Vallejo, Esther Liceras-Liceras, Jesús Chato-Astrain, Oscar Dario García-García, David Sánchez-Porras, Paula Ávila-Fernández, Miguel Etayo-Escanilla, Blanca Quijano, Elisabet Aguilar, Antonio Campos, Gloria Carmona, Miguel Alaminos
BMJ Open, 2024
IntroductionThe current gold standard treatment for patients with orofacial clefts is surgical repair of the palatal defect (uranostaphylorrhaphy), which is associated with growth defects and hypoplasia of the maxillofacial structures. This trial aims to evaluate the potential of a bioengineered artificial palate mucosa, created through tissue engineering with autologous stromal and epithelial cells and nanostructured fibrin–agarose biomaterials, to enhance treatment outcomes for patients with unilateral cleft lip and palate.Methods and analysisThis phase I-IIa clinical trial aims to evaluate the feasibility and biosafety of a procedure involving grafting bioartificial palate mucosa onto the areas of denudated bone in patients undergoing uranostaphylorrhaphy. The control patients will undergo standard surgical treatment. Five patients will be included in the first biosafety phase. In the second phase, 10 patients will be randomly assigned to the intervention or control group (1:1). The intervention group will undergo standard surgical treatment followed by the application of autologous bioartificial palate mucosa. Feasibility will be analysed at the time of surgery. Nine postimplant visits will be scheduled over a 2-year follow-up period, in which local and systemic biosafety will be investigated by determining graft evolution, including signs of necrosis, rejection, inflammation and patient factors. Preliminary signs of efficiency will be explored by sequentially evaluating craniomaxillofacial development, hearing impairment, speech capability and quality of life of the family. The research will be published in journals and posted in the relevant repositories when available.Ethics and disseminationThis study has been approved by the Committee of Ethics in Research with Medicinal Products (CEIm) and authorised by the Spanish Medicines Agency (AEMPS). The results of this study will be published in peer-reviewed journals.Trial registration numberNCT06408337; ClinicalTrials.gov: EuclinicalTrials. eu: 2023-506913-23-00.
Histological, histochemical, and immunohistochemical characterization of NANOULCOR nanostructured fibrin-agarose human cornea substitutes generated by tissue engineering
Olimpia Ortiz-Arrabal, Cristina Blanco-Elices, Carmen González-Gallardo, David Sánchez-Porras, Miguel Etayo-Escanilla, Paula Ávila Fernández, Jesús Chato-Astrain, Óscar-Darío García-García, Ingrid Garzón, Miguel Alaminos
BMC Medicine, 2024
BACKGROUND: Human artificial corneas (HAC) generated by tissue engineering recently demonstrated clinical usefulness in the management of complex corneal diseases. However, the biological mechanisms associated to their regenerative potential need to be elucidated. METHODS: In the present work, we generated HAC using nanostructured fibrin-agarose biomaterials with cultured corneal epithelial and stromal cells, and we compared the structure and histochemical and immunohistochemical profiles of HAC with control native corneas (CTR-C) and limbus (CTR-L) to determine the level of biomimicry of the HAC with these two native organs. RESULTS: HAC tissues consisted of a stratified epithelium and a cellular stromal substitute. The interface between stroma and epithelium was similar to that of CTR-C, without the finger-shaped palisades of Vogt found in CTR-L, and contained a poorly developed basement membrane as determined by PAS histochemistry. Analysis of the stromal layer revealed that HAC contained significantly lower amounts of extracellular matrix components (collagen, proteoglycans, decorin, keratocan, and lumican) than CTR-C and CTR-L, with all samples being devoid of elastic and reticular fibers. At the epithelial level, HAC were strongly positive for several cytokeratins, although KRT5 was lower in HAC as compared to CTR-C and CTR-L. The expression of crystallin lambda was lower in HAC than in control tissues, whereas crystallin alpha-a was similar in HAC and CTR-C. No differences were found among HAC and controls for the cell-cell junction proteins CX43 and TJP1. When specific markers were analyzed, we found that HAC expression profile of KRT3, KRT19, KRT15, and ΔNp63 was more similar to CTR-L than to CTR-C. CONCLUSIONS: These results suggest that HAC generated in the laboratory could be structurally and functionally more biomimetic to the structure found at the corneal limbus than to the central cornea, and open the door to the use of these artificial tissues in patients with limbal deficiency.
Spatiotemporal characterization of extracellular matrix maturation in human artificial stromal-epithelial tissue substitutes
Paula Ávila-Fernández, Miguel Etayo-Escanilla, David Sánchez-Porras, Ricardo Fernández-Valadés, Fernando Campos, Ingrid Garzón, Víctor Carriel, Miguel Alaminos, Óscar Darío García-García, Jesús Chato-Astrain
BMC Biology, 2024
Background Tissue engineering techniques offer new strategies to understand complex processes in a controlled and reproducible system. In this study, we generated bilayered human tissue substitutes consisting of a cellular connective tissue with a suprajacent epithelium (full-thickness stromal-epithelial substitutes or SESS) and human tissue substitutes with an epithelial layer generated on top of an acellular biomaterial (epithelial substitutes or ESS). Both types of artificial tissues were studied at sequential time periods to analyze the maturation process of the extracellular matrix. Results Regarding epithelial layer, ESS cells showed active proliferation, positive expression of cytokeratin 5, and low expression of differentiation markers, whereas SESS epithelium showed higher differentiation levels, with a progressive positive expression of cytokeratin 10 and claudin. Stromal cells in SESS tended to accumulate and actively synthetize extracellular matrix components such as collagens and proteoglycans in the stromal area in direct contact with the epithelium (zone 1), whereas these components were very scarce in ESS. Regarding the basement membrane, ESS showed a partially differentiated structure containing fibronectin-1 and perlecan. However, SESS showed higher basement membrane differentiation, with positive expression of fibronectin 1, perlecan, nidogen 1, chondroitin-6-sulfate proteoglycans, agrin, and collagens types IV and VII, although this structure was negative for lumican. Finally, both ESS and SESS proved to be useful tools for studying metabolic pathway regulation, revealing differential activation and upregulation of the transforming growth factor-β pathway in ESS and SESS. Conclusions These results confirm the relevance of epithelial-stromal interaction for extracellular matrix development and differentiation, especially regarding basement membrane components, and suggest the usefulness of bilayered artificial tissue substitutes to reproduce ex vivo the extracellular matrix maturation and development process of human tissues. Graphical Abstract
A new 3D model of L929 fibroblasts microtissues uncovers the effects of Bothrops erythromelas venom and its antivenom
F. R. S. Andrade, E. L. da Silva, A. D. Marinho, A. C. X. Oliveira, D. Sánchez-Porras, F. Bermejo-Casares, R. C. Montenegro, V. Carriel, H. S. A. Monteiro, R. J. B. Jorge
Archives of Toxicology, 2024
Comparison of Printable Biomaterials for Use in Neural Tissue Engineering: An In Vitro Characterization and In Vivo Biocompatibility Assessment
Miguel Etayo-Escanilla, Noelia Campillo, Paula Ávila-Fernández, José Manuel Baena, Jesús Chato-Astrain, Fernando Campos, David Sánchez-Porras, Óscar Darío García-García, Víctor Carriel
Polymers, 2024
A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer
Paula Ávila-Fernández, Miguel Etayo-Escanilla, David Sánchez-Porras, Cristina Blanco-Elices, Fernando Campos, Víctor Carriel, Óscar Darío García-García, Jesús Chato-Astrain
Gels, 2024
Intratumoral injection of melatonin enhances tumor regression in cell line-derived and patient-derived xenografts of head and neck cancer by increasing mitochondrial oxidative stress
Laura Martinez-Ruiz, Javier Florido, César Rodriguez-Santana, Alba López-Rodríguez, Ana Guerra-Librero, Beatriz I. Fernández-Gil, Patricia García-Tárraga, José Manuel Garcia-Verdugo, Felix Oppel, Holger Sudhoff, David Sánchez-Porras, Amadeo Ten-Steve, José Fernández-Martínez, Pilar González-García, Iryna Rusanova, Darío Acuña-Castroviejo, Víctor Carriel, Germaine Escames
Biomedicine and Pharmacotherapy, 2023
ROD2 domain filamin C missense mutations exhibit a distinctive cardiac phenotype with restrictive/hypertrophic cardiomyopathy and saw-tooth myocardium
Francisco José Bermúdez-Jiménez, Víctor Carriel, Juan José Santos-Mateo, Adrián Fernández, Soledad García-Hernández, Karina Analía Ramos, Jesús Piqueras-Flores, Eva Cabrera-Romero, Roberto Barriales-Villa, Luis de la Higuera Romero, Juan Emilio Alcalá López, Juan Ramón Gimeno Blanes, David Sánchez-Porras, Fernando Campos, Miguel Alaminos, José Manuel Oyonarte-Ramírez, Miguel Álvarez, Luis Tercedor, Andreas Brodehl, Juan Jiménez-Jáimez
Revista Espanola De Cardiologia, 2023
Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues
David Sánchez-Porras, Daniel Durand-Herrera, Ramón Carmona, Cristina Blanco-Elices, Ingrid Garzón, Michela Pozzobon, Sebastián San Martín, Miguel Alaminos, Óscar Darío García-García, Jesús Chato-Astrain, Víctor Carriel
Cells, 2023
Histochemical and Immunohistochemical Methods for the Identification of Proteoglycans
David Sánchez-Porras, Juan Varas, Carlos Godoy-Guzmán, Fabiola Bermejo-Casares, Sebastián San Martín, Víctor Carriel
Methods in Molecular Biology, 2023
Tissue Fixation and Processing for the Histological Identification of Lipids
David Sánchez-Porras, Fabiola Bermejo-Casares, Ramón Carmona, Tamara Weiss, Fernando Campos, Víctor Carriel
Methods in Molecular Biology, 2023
A novel 3D biofabrication strategy to improve cell proliferation and differentiation of human Wharton’s jelly mesenchymal stromal cells for cell therapy and tissue engineering
Cristina Blanco-Elices, Roke Iñaki Oruezabal, David Sánchez-Porras, Jesús Chato-Astrain, Fernando Campos, Miguel Alaminos, Ingrid Garzón, Antonio Campos
Frontiers in Bioengineering and Biotechnology, 2023
Identification of histological threshold concepts in health sciences curricula: Students' perception
Miguel A. Martin‐Piedra, Salvador Saavedra‐Casado, Antonio Santisteban‐Espejo, Fernando Campos, Jesus Chato‐Astrain, Oscar Dario Garcia‐Garcia, David Sanchez‐Porras, Juan de Dios Luna del Castillo, Ismael Angel Rodriguez, Antonio Campos
Anatomical Sciences Education, 2023
Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts
Óscar Darío García-García, Marwa El Soury, Fernando Campos, David Sánchez-Porras, Stefano Geuna, Miguel Alaminos, Giovanna Gambarotta, Jesús Chato-Astrain, Stefania Raimondo, Víctor Carriel
Frontiers in Bioengineering and Biotechnology, 2023
Histological Profiling of the Human Umbilical Cord: A Potential Alternative Cell Source in Tissue Engineering
Cristina Blanco-Elices, Jesús Chato-Astrain, Alberto González-González, David Sánchez-Porras, Víctor Carriel, Ricardo Fernández-Valadés, María del Carmen Sánchez-Quevedo, Miguel Alaminos, Ingrid Garzón
Journal of Personalized Medicine, 2022
NANOSTRUCTURED FIBRIN-BASED HYDROGEL MEMBRANES FOR USE AS AN AUGMENTATION STRATEGY IN ACHILLES TENDON SURGICAL REPAIR IN RATS
, D González-Quevedo, D Sánchez-Porras, Ó-D García-García, J Chato-Astrain, M Díaz-Ramos, A Campos, V Carriel, F Campos
European Cells and Materials, 2022
Improvement of cell culture methods for the successful generation of human keratinocyte primary cell cultures using egf-loaded nanostructured lipid carriers
Jesús Chato-Astrain, David Sánchez-Porras, Óscar Darío García-García, Claudia Vairo, María Villar-Vidal, Silvia Villullas, Indalecio Sánchez-Montesinos, Fernando Campos, Ingrid Garzón, Miguel Alaminos
Biomedicines, 2021
Generation of a biomimetic substitute of the corneal limbus using decellularized scaffolds
David Sánchez-Porras, Manuel Caro-Magdaleno, Carmen González-Gallardo, Óscar Darío García-García, Ingrid Garzón, Víctor Carriel, Fernando Campos, Miguel Alaminos
Pharmaceutics, 2021
Generation and evaluation of novel biomaterials based on decellularized sturgeon cartilage for use in tissue engineering
Olimpia Ortiz-Arrabal, Ramón Carmona, Óscar-Darío García-García, Jesús Chato-Astrain, David Sánchez-Porras, Alberto Domezain, Roke-Iñaki Oruezabal, Víctor Carriel, Antonio Campos, Miguel Alaminos
Biomedicines, 2021
Ex vivo generation and characterization of human hyaline and elastic cartilaginous microtissues for tissue engineering applications
David Sánchez-Porras, Daniel Durand-Herrera, Ana B. Paes, Jesús Chato-Astrain, Rik Verplancke, Jan Vanfleteren, José Darío Sánchez-López, Óscar Darío García-García, Fernando Campos, Víctor Carriel
Biomedicines, 2021
Evaluation of marine agarose biomaterials for tissue engineering applications
Ainhoa Irastorza-Lorenzo, David Sánchez-Porras, Olimpia Ortiz-Arrabal, María José de Frutos, Emilio Esteban, Javier Fernández, Agustín Janer, Antonio Campos, Fernando Campos, Miguel Alaminos
International Journal of Molecular Sciences, 2021
Histological, biomechanical, and biological properties of genipin-crosslinked decellularized peripheral nerves
Óscar Darío García-García, Marwa El Soury, David González-Quevedo, David Sánchez-Porras, Jesús Chato-Astrain, Fernando Campos, Víctor Carriel
International Journal of Molecular Sciences, 2021
Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering
Jesús Chato-Astrain, Isabel Chato-Astrain, David Sánchez-Porras, Óscar-Darío García-García, Fabiola Bermejo-Casares, Claudia Vairo, María Villar-Vidal, Garazi Gainza, Silvia Villullas, Roke-Iñaki Oruezabal, Ángela Ponce-Polo, Ingrid Garzón, Víctor Carriel, Fernando Campos, Miguel Alaminos
Journal of Nanobiotechnology, 2020
Erratum: Blanco-elices, C. et al. in vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. running title: Fibrin–agarose functionalized scaffolds. (Materials 2020, 13, 1692)
Cristina Blanco-Elices, Enrique España-Guerrero, Miguel Mateu-Sanz, David Sánchez-Porras, Óscar Darío García-García, María del Carmen Sánchez-Quevedo, Ricardo Fernández-Valadés, Miguel Alaminos, Miguel Ángel Martín-Piedra, Ingrid Garzón
Materials, 2020
Improving the regenerative microenvironment during tendon healing by using nanostructured fibrin/agarose-based hydrogels in a rat Achilles tendon injury model
David González-Quevedo, Miriam Díaz-Ramos, Jesús Chato-Astrain, David Sánchez-Porras, Iskandar Tamimi, Antonio Campos, Fernando Campos, Víctor Carriel
Bone and Joint Journal, 2020
Evaluation of myopic cornea lenticules. A histochemical and clinical correlation: Histology of the myopic cornea
J.A. Rodriguez-Pozo, J.F. Ramos-Lopez, M.C. Gonzalez-Gallardo, F. Campos, D. Sanchez-Porras, S. Oyonarte, R.I. Oruezabal, A. Campos, M.A. Martin-Piedra, M. Alaminos
Experimental Eye Research, 2020
Evaluation of Fibrin-Agarose Tissue-Like Hydrogels Biocompatibility for Tissue Engineering Applications
Fernando Campos, Ana Belen Bonhome-Espinosa, Jesús Chato-Astrain, David Sánchez-Porras, Óscar Darío García-García, Ramón Carmona, Modesto T. López-López, Miguel Alaminos, Víctor Carriel, Ismael A. Rodriguez
Frontiers in Bioengineering and Biotechnology, 2020
An Evolutive and Scientometric Research on Tissue Engineering Reviews
Miguel Angel Martin-Piedra, Antonio Santisteban-Espejo, Jose Antonio Moral-Munoz, Fernando Campos, Jesus Chato-Astrain, Oscar Dario Garcia-Garcia, David Sanchez-Porras, Antonio Campos
Tissue Engineering Part A, 2020
In vitro generation of novel functionalized biomaterials for use in oral and dental regenerative medicine applications. Running title: Fibrin-agarose functionalized scaffolds
Cristina Blanco-Elices, Enrique España-Guerrero, Miguel Mateu-Sanz, David Sánchez-Porras, Óscar García-García, María Sánchez-Quevedo, Ricardo Fernández-Valadés, Miguel Alaminos, Miguel Martín-Piedra, Ingrid Garzón
Materials, 2020

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