Ultrasound Thawing Enhances Myofibrillar Protein Integrity and Quality Attributes of Common Carp (Cyprinus carpio) Fillets Mohammad Mehdi Asgari, Mina Esmaeili, Abbas Zamani, Seyed Vali Hosseini Food Science and Nutrition, 2026 Freezing and thawing are critical techniques for preserving seafood quality and extending shelf life. This study evaluated the effects of ultrasound‐assisted (UT), water immersion (WT), and microwave (MT) thawing on physicochemical, textural, and structural quality attributes of common carp ( Cyprinus carpio ) fillets and their myofibrillar protein properties. Results demonstrated that ultrasound thawing (UT) most effectively maintained pH and minimized protein aggregation, reducing myofibrillar protein particle size by approximately 49%, significantly lowering turbidity, and decreasing cooking loss by about 10% compared with water immersion thawing (WT). In contrast, water immersion thawing resulted in the greatest moisture loss, whereas ultrasound and microwave thawing better preserved water‐holding capacity. Scanning electron microscopy revealed that WT and MT caused minor muscle damage, whereas UT better preserved protein integrity, showing significantly reduced aggregation and higher zeta potential compared with MT ( p < 0.05). SDS‐PAGE confirmed that no treatments altered the primary protein structure. Based on overall quality retention, ultrasound thawing is recommended as the optimal thawing method for common carp fillets.
Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species Abbas Zamani, Maryam Khajavi, Abdolmohammad Abedian Kenari, Masoumeh Haghbin Nazarpak, Atefeh Solouk, Mina Esmaeili, Enric Gisbert Animals, 2023 This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca+2 and surfactants and decreased by oxidizing agents, Cu+2, Zn+2, and Co+2 (p < 0.05). However, univalent ions Na+ and K+ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species.
Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering Maryam Khajavi, Abdolmajid Hajimoradloo, Mojgan Zandi, Mohamad Pezeshki‐Modaress, Shahin Bonakdar, Abbas Zamani Journal of Biomedical Materials Research Part A, 2021 Here, engineered cartilage‐like scaffold using an extracellular matrix (ECM) from sturgeon fish cartilage provided a chondroinductive environment to stimulate cartilaginous matrix synthesis in human adipose stem cells (hASCs). Three dimensional porous and degradable fish cartilage ECM‐derived scaffold (FCS) was produced using a protocol containing chemical decellularization, enzymatic solubilization, freeze‐drying and EDC‐crosslinking treatments and the effect of different ECM concentrations (10, 20, 30, and 40 mg/ml) on prepared scaffolds was investigated through physical, mechanical and biological analysis. The histological and scanning electron microscopy analysis revealed the elimination of the cell fragments and a 3‐D interconnected porous structure, respectively. Cell viability assay displayed no cytotoxic effects. The prepared porous constructs of fish cartilage ECM were seeded with hASCs for 21 days and compared to collagen (Col) and collagen‐10% hyaluronic acid (Col‐HA) scaffolds. Cell culture results evidenced that the fabricated scaffolds could provide a proper 3‐D structure to support the adhesion, proliferation and chondrogenic differentiation of hASCs considering the synthesis of specific proteins of cartilage, collagen type II (Col II) and aggrecan (ACAN). Based on the results of the present study, it can be concluded that the porous scaffold derived from fish cartilage ECM possesses an excellent potential for cartilage tissue engineering.
Assessment of chemical and bacterial indices of common carp (Cyprinus carpio) surimi under various concentrations of basil (ocimum basilicum) extract during storage in refrigerator Iranian Journal of Nutrition Sciences and Food Technology, 2021
Acid hydrolysis of gelatin extracted from cow skin: Properties and potential for use as a source of small peptides and free amino acids for broiler chickens K. Nouri, S. Khalaji, A. Zamani, A. Saki Animal Production Science, 2021 Context Acid hydrolysis of animal and plant protein ingredients can generate beneficial and cost-effective peptides and free amino acids for use in livestock feed. Aims The aim was to determine whether a cow-skin gelatin (CSG) hydrolysate, rich in low-molecular-weight peptides and free amino acid and produced through acid (4 M HCl) hydrolysis, improved the digestibility of CSG and performance of broilers. Methods Day-old Ross 308 chicks (n = 320) were allocated to four treatments (control, maize–soy basal diet; CSG at 92 g/kg basal diet; acid-hydrolysed CSG (AHCSG) at 45 and 92 g/kg basal diet) in a completely randomised design with eight replicate pens per treatment and 10 chicks in each pen. Molecular weights of CSG and AHCSG were characterised by SDS–PAGE, and protein concentration and degree of hydrolysis of AHCSG determined. Broilers were assessed for performance measures and intestinal and serum characteristics. Key results The AHCSG had a protein concentration of 84.3%, with a degree of hydrolysis of 66.5%. Respective molecular weights of CSG and AHCSG were 20–128 kDa and 3.5–10 kDa. Bodyweight gain and feed intake were dramatically (P ≤ 0.001) reduced and feed conversion ratio increased with inclusion of CSG and especially AHCSG in the diet. Inclusion of AHCSG reduced (P &lt; 0.05) ileal digesta viscosity compared with CSG, and reduced (P &lt; 0.05) plasma uric acid concentration, villi height and crypt depth compared with the CSG and control diets. AHCSG inclusion in the diet reduced protein digestibility by ~25% (at 45 g/kg) and 50% (at 92 g/kg) compared with the control; CSG also reduced protein digestibility by ~50%. The Salmonella population of ceca was reduced (P = 0.05) with the diet containing 92 g AHCSG/kg compared with the CSG and control diets. Trypsin activity was not affected by diet, but total alkaline protease activity was reduced (P &lt; 0.01) at days 35 of age by inclusion of AHCSG in the diet. Carcass and breast relative weight, and breast fillet crude protein were lower (P &lt; 0.05) for broilers fed diets containing CSG or AHCSG than the control. Conclusions Acid hydrolysis of CSG produced large quantities of free amino acids and small peptides, but this did not improve the performance of broiler chickens compared with CSG. Performance was similarly poor with dietary inclusion of AHCSG and CSG relative to the control diet without CSG products. Implications Although large quantities of free amino acids and small peptides were produced by acid hydrolysis of CSG, further research is needed to understand the possible problems with acid hydrolysis and why it negatively affects broiler performance.
Utilization of grape seed oil as a dietary lipid source in rainbow trout (Oncorhynchus mykiss) diets Abbas Zamani, Maryam Khajavi, Aboozar Moafi, Enric Gisbert Journal of Applied Aquaculture, 2021 A 60-day feeding trial was conducted to determine the effects of different levels of grape seed oil (GO) on growth performance, digestive enzyme activity, fillet proximate and fatty acid composition of rainbow trout (Oncorhynchus mykiss) juveniles (40.17 ± 0.04 g). Five experimental diets were formulated where fish oil (FO) was replaced with 0 (D1), 25 (D2), 50 (D3), 75 (D4) and 100 (D5) % GO. The growth performance was significantly improved with increasing GO levels up to 50% after which fish growth declined (P < .05). Fillet fatty acid composition was showed significantly highest n-6 PUFA levels with increasing GO in diets, while n-3 HUFA levels especially EPA and DHA were significantly decreased (P < .05). Fish fed on diets containing higher levels of GO revealed a decrease in α-amylase activity, whereas trypsin, total alkaline protease, and lipase activities increased significantly with increasing GO levels up to 50% and then decreased. Based on the findings of the present study, it could be concluded that GO could be included in diets up to 50% where it had the best performance over the other diets tested in the present experiment.
Evaluation of a bacterial single-cell protein in compound diets for rainbow trout (Oncorhynchus mykiss) fry as an alternative protein source Abbas Zamani, Maryam Khajavi, Masoumeh Haghbin Nazarpak, Enric Gisbert Animals, 2020 A 60-day trial was conducted in rainbow trout (Oncorhynchus mykiss) fry (initial weight = 2.5 ± 0.6 g) to evaluate the potential use of a bacterial single-cell protein (SCP) as an alternative protein source. Five experimental diets with different levels of fishmeal replacement (0, 25, 50, 75 and 100%) and no amino acid supplementation were tested. At the end of the trial, we found that fry fed diets, replacing 25 and 50% of fishmeal with bacterial SCP, were 9.1 and 21.8% heavier, respectively, than those fed the control diet (p < 0.05), while Feed Conversion Ratio (FCR) values were also lower in comparison to the reference group. These results were also supported by Protein Efficiency Ratio (PER) and Lipid Efficiency Ratio (LER) values that improved in fish fed diets replacing 50% fishmeal by bacterial SCP. The inclusion of SCP enhanced Feed intake (FI) (p < 0.05), although FI was reduced at higher inclusion levels (>50%), which was associated to feed palatability. High levels of bacterial SCP (>50%) affected the muscular amino acid and fatty acid profiles, imbalances that were associated to their dietary content. The broken-line regression analysis using muscle DHA content and weight gain data showed that the maximum levels of fishmeal replacement by bacterial SCP were 46.9 and 52%, respectively.
Comparison of the Effect of Caspian kutum trypsin (Rutilus frisii kutum) with commercial enzymes on the functional and antioxidant properties of soy protein hydrolysates Journal of Food Science and Technology Iran, 2019
Assessment of digestive enzymes activity during the fry development of Rainbow Trout, Oncorhynchus mykiss: From hatching to primary stages after yolk sac absorption Iranian Journal of Fisheries Sciences, 2011
Ultrasound Thawing Enhances Myofibrillar Protein Integrity and Quality Attributes of Common Carp ( Cyprinus carpio ) Fillets MM Asgari, M Esmaeili, A Zamani, SV Hosseini Food Science & Nutrition 14 (5), e71833 , 2026 2026
Effect of some physical and chemical factors on the trypsin activity from the viscera of rainbow trout (Oncorhynchus mykiss) A Zamani ISFJ 34 (6), 85-98 , 2026 2026
Preparation and characterization of drug delivery system based on biodegradable polymer polycaprolactone (PCL) and hydrogel from beluga (Husohuso) cartilage M Khajavi, A Hajimoradloo, M Zandi, M Pezeshki-Modaress, A Zamani, ... Journal of Fisheries Science and Technology 9 (3), 201-211 , 2025 2025
Effects of fish meal replacement with black soldier fly larvae meal in the diet of Pacific white shrimp, Litopenaeus vannamei post-larvae on growth performance and digestive … A Zamani, M Jafari Aquatic Animals Nutrition 10 (4), 19-34 , 2024 2024
The effect of different levels of carrot powder in angel fish (Pterophyllum scalare) diet on the growth performance and activity of digestive and antioxidant enzymes A Zamani Journal of Utilization and Cultivation of Aquatics , 2024 2024
Effect of enzyme extract derived from fish pyloric appendages on growth performance and biochemical composition of rainbow trout fry (Oncorhynchus mykiss) P Heshmatzad, A Zamani, H Cheraghi Journal of Utilization and Cultivation of Aquatics 13 (3), 183-195 , 2024 2024
Preliminary evaluation of fish cartilage as a promising biomaterial in cartilage tissue engineering A Zamani, M Khajavi, MH Nazarpak, A Solouk, M Atef Annals of Anatomy-Anatomischer Anzeiger 253, 152232 , 2024 2024 Citations: 5
The evaluation of bacterial single cell protein on performance, digestive enzymes activity, gut histology and gut microbiota of rainbow trout (Oncorhynchus mykiss) fry A Zamani, S Khalaji Journal of Fisheries Science and Technology 13 (1), 398-411 , 2024 2024 Citations: 4
Effects of natural carotenoid (carrot and red bell pepper) on growth performance, digestive enzymes and antioxidant enzymes in koi, Cyprinus rubrofuscus A Zamani Aquatic Animals Nutrition 9 (4), 1-13 , 2023 2023 Citations: 2
Evaluation of curcumin release from wound dressing based on gelatin and sturgeon-derived chondroitin sulfate M Soltani, MH Nazarpak, A Zamani, A Solouk Materials Today Communications 35, 106167 , 2023 2023 Citations: 14
Physicochemical and biochemical properties of trypsin-like enzyme from two sturgeon species A Zamani, M Khajavi, A Abedian Kenari, M Haghbin Nazarpak, A Solouk, ... Animals 13 (5), 853 , 2023 2023 Citations: 24
Evaluation of biochemical properties of the purified trypsin from common kilka (Clupeonella cultriventris caspia) intestine A Zamani, M Rezaei, R Madani Journal of Utilization and Cultivation of Aquatics 11 (3), 41-56 , 2022 2022 Citations: 1
Chymotrypsin activity extracted from beluga (Huso huso) intestine under some physical and chemical factors A Zamani, M Khajavi Iranian Scientific Fisheries Journal 31 (3), 17-28 , 2022 2022
Effects of dietary inclusion of yellow mealworm (Tenebrio molitor) meal on growth performance and proteolytic enzymes activity of koi carp (Cyprinus rubrofuscus) juvenile A Zamani, M Goli Journal of Fisheries Science and Technology 10 (4), 410-422 , 2021 2021 Citations: 3
گیاهان دارویی: منبعی امیدبخش در پیشگیری و درمان بیماریهای آبزیان زمانی, خواجوی مجله بهره برداری و پرورش آبزیان 10 (3), 27-47 , 2021 2021
Medicinal plants: A promising source in the prevention and treatment of aquatic animal diseases A Zamani, M Khajavi Journal of Utilization and Cultivation of Aquatics 10 (3), 27-47 , 2021 2021
Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering M Khajavi, A Hajimoradloo, M Zandi, M Pezeshki‐Modaress, S Bonakdar, ... Journal of Biomedical Materials Research Part A 109 (9), 1737-1750 , 2021 2021 Citations: 38
Utilization of grape seed oil as a dietary lipid source in rainbow trout ( Oncorhynchus mykiss ) diets A Zamani, M Khajavi, A Moafi, E Gisbert Journal of Applied Aquaculture 33 (2), 126-149 , 2021 2021 Citations: 9
Assessment of chemical and bacterial indices of common carp ( Cyprinus carpio ) surimi under various concentrations of basil ( Ocimum basilicum ) extract during … A Zamani, Z Abaei, F Abaei 2021 Citations: 3
Acid hydrolysis of gelatin extracted from cow skin: properties and potential for use as a source of small peptides and free amino acids for broiler chickens K Nouri, S Khalaji, A Zamani, A Saki Animal Production Science 61 (4), 399-411 , 2020 2020 Citations: 7
MOST CITED SCHOLAR PUBLICATIONS
Effect of starvation and refeeding on digestive enzyme activities in juvenile roach, Rutilus rutilus caspicus M Abolfathi, A Hajimoradloo, R Ghorbani, A Zamani Comparative Biochemistry and Physiology Part A: Molecular & Integrative … , 2012 2012 Citations: 131
Assessment of digestive enzymes activity during the fry development of the endangered Caspian brown trout Salmo caspius A Zamani, A Hajimoradloo, R Madani, M Farhangi Journal of Fish Biology 75 (4), 932-937 , 2009 2009 Citations: 72
Evaluation of a Bacterial Single-Cell Protein in Compound Diets for Rainbow Trout ( Oncorhynchus mykiss ) Fry as an Alternative Protein Source A Zamani, M Khajavi, MH Nazarpak, E Gisbert Animals 10 (9), 1676 , 2020 2020 Citations: 47
Compensatory growth in juvenile roach Rutilus caspicus : effect of starvation and re‐feeding on growth and digestive surface area M Abolfathi, A Hajimoradloo, R Ghorbani, A Zamani Journal of Fish Biology 81 (6), 1880-1890 , 2012 2012 Citations: 43
Fish cartilage: A promising source of biomaterial for biological scaffold fabrication in cartilage tissue engineering M Khajavi, A Hajimoradloo, M Zandi, M Pezeshki‐Modaress, S Bonakdar, ... Journal of Biomedical Materials Research Part A 109 (9), 1737-1750 , 2021 2021 Citations: 38
Trypsin from unicorn leatherjacket ( Aluterus monoceros ) pyloric caeca: purification and its use for preparation of fish protein hydrolysate with antioxidative activity A Zamani, S Benjakul Journal of the Science of Food and Agriculture 96 (3), 962-969 , 2016 2016 Citations: 37
Feathers of three waterfowl bird species from Northern Iran for heavy metals biomonitoring E Solgi, E Mirzaei-Rajeouni, A Zamani Bulletin of environmental contamination and toxicology 104 (6), 727-732 , 2020 2020 Citations: 32
Physicochemical and biochemical properties of trypsin-like enzyme from two sturgeon species A Zamani, M Khajavi, A Abedian Kenari, M Haghbin Nazarpak, A Solouk, ... Animals 13 (5), 853 , 2023 2023 Citations: 24
Assessment of digestive enzymes activity during the fry development of Rainbow trout, Oncorhynchus mykiss : from hatching to primary stages after yolk sac … F Golchinfar, A Zamani, A Hajimoradloo, R Madani 2011 Citations: 23
Trypsin Enzyme from Viscera of Common Kilka ( Clupeonella cultriventris caspia ): Purification, Characterization, and Its Compatibility with Oxidants and Surfactants A Zamani, M Rezaei, R Madani, M Habibi Rezaie Journal of Aquatic Food Product Technology 23 (3), 237-252 , 2014 2014 Citations: 19
Evaluation of curcumin release from wound dressing based on gelatin and sturgeon-derived chondroitin sulfate M Soltani, MH Nazarpak, A Zamani, A Solouk Materials Today Communications 35, 106167 , 2023 2023 Citations: 14
Antioxidative Activitiy of Protein Hydrolysate from the Muscle of Common Kilka ( Clupeonella cultriventris caspia ) Prepared Using the Purified Trypsin from Common … A Zamani, R Madani, M Rezaei, S Benjakul Journal of Aquatic Food Product Technology 26 (1), 2-16 , 2017 2017 Citations: 10
Utilization of grape seed oil as a dietary lipid source in rainbow trout ( Oncorhynchus mykiss ) diets A Zamani, M Khajavi, A Moafi, E Gisbert Journal of Applied Aquaculture 33 (2), 126-149 , 2021 2021 Citations: 9
Evaluation of a bacterial single-cell protein in compound diets for rainbow trout (Oncorhynchus mykiss) fry as an alternative protein source. Animals 10, 1676 A Zamani, M Khajavi, MH Nazarpak, E Gisbert 2020 Citations: 8
Effects of gelatin prepared from calf bones rich in phosphorus on broiler performance, bone characteristics and digestive enzymes activity F Beyranvand, S Khalaji, A Zamani, M Manafi British poultry science 60 (1), 31-38 , 2019 2019 Citations: 8
Acid hydrolysis of gelatin extracted from cow skin: properties and potential for use as a source of small peptides and free amino acids for broiler chickens K Nouri, S Khalaji, A Zamani, A Saki Animal Production Science 61 (4), 399-411 , 2020 2020 Citations: 7
Preliminary evaluation of fish cartilage as a promising biomaterial in cartilage tissue engineering A Zamani, M Khajavi, MH Nazarpak, A Solouk, M Atef Annals of Anatomy-Anatomischer Anzeiger 253, 152232 , 2024 2024 Citations: 5
In-vitro effects of biochemical factors on trypsin activity from intestine and pyloric caeca of common kilka (Clupeonella cultriventris caspia) for inhibition of belly bursting A Zamani, M Rezaei, R Madani ISFJ 20 (4), 53-62 , 2012 2012 Citations: 5
Comparison of digestive enzyme activity in the stomach, pyloric caeca and intestine in diploid and triploid female of rainbow trout (Oncorhynchus mykiss) A Zamani, A Haji Moradlo, R Madani, A Johari, M Kalbasi, M Farhangi Iranian Scientific Fisheries Journal 15 (2), 29-36 , 2006 2006 Citations: 5
The evaluation of bacterial single cell protein on performance, digestive enzymes activity, gut histology and gut microbiota of rainbow trout (Oncorhynchus mykiss) fry A Zamani, S Khalaji Journal of Fisheries Science and Technology 13 (1), 398-411 , 2024 2024 Citations: 4