Lucia Paolini

@unibs.it

Department of Medical and Surgical Specialties, Radiological Sciences and Public Health
University of Brescia

Lucia Paolini


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https://researchid.co/lucia.paolini
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Scopus Publications

Scopus Publications

  • A reliable high-throughput OECT-based bioelectronic system enabling pan-specific vesicle quantification
    Giulia Frusconi, Angelo Musicò, Andrea Zendrini, Zsolt M. Kovács-Vajna, Lucia Paolini, Paolo Bergese, Fabrizio Torricelli
    Biosensors and Bioelectronics X, 2026
    Next-generation extracellular vesicle (EV) technologies are transforming biomanufacturing and functional product development. Quantifying EVs based on their membrane protein content is crucial for ensuring consistent yield, composition, and functional performance in manufacturing pipelines. Existing methods measure EV size and concentration but provide no information on membrane protein presence, whereas immunoaffinity approaches depend on specific markers that may be inconsistently expressed due to EV heterogeneity. Here we demonstrate a reliable bioelectronic system for pan-specific, high-throughput total EV quantification, achieving 12 independent parallel analysis in less than 15 min, with false-positive and false-negative rates below 1%. The system features multiple channel-functionalized arrays of organic electrochemical transistors (OECTs) in a 3 × 4 matrix configuration. Click chemistry enables real-time detection of EVs with membrane proteins, with a limit of identification of 6 × 106 particles ml−1 and a dynamic range spanning three orders of magnitude. By integrating functionalized OECT arrays with 3D-printed fluidic devices into a low-cost, disposable cartridge operated by a compact silicon-based electronic reader, the system enables rapid, reliable, and pan-specific total EV quantification in a user-friendly format, opening opportunities for in-line quality control and scalable implementation in biomanufacturing and pharmaceutical processes.
  • FOURIER-TRANSFORM INFRARED (FT-IR) SPECTROSCOPY OF PLASMA AS A LIQUID BIOPSY FOR MONITORING ACUTE MYELOID LEUKEMIA AND EXTRAMEDULLARY RELAPSE FOLLOWING ALLO-HSCT
    Alessandro Leoni
    Haematologica, 2026
    Relapse of Acute Myeloid Leukemia (AML) remain a major cause of treatment failure. Among relapse patterns, extramedullary disease, referred to as myeloid sarcoma, representing a rare but clinically significant scenario, characterized by infiltration of leukemic blasts in tissues outside the bone marrow. Diagnosis is challenging and requires a multimodal approach integrating imaging, histopathology and immunophenotyping to confirm the myeloid origin of blasts. Prognosis is influenced by localization, disease burden and characteristic and response to therapy. In this single-Centre study, we employed a liquid biopsy approach based on Fourier-Transform Infrared (FT-IR) spectroscopy to analyze plasma samples from AML patients collected at diagnosis, pre- and post-allogenic hematopoietic stem cell transplantation (allo-HSCT), bone marrow (BM) relapse, and myeloid sarcoma, as well as from healthy controls. Spectral data were normalized using standard normal variate and analyzed with multivariate and machine-learning approaches (Random Forest) with leave-one-patient-out cross-validation. The overall comparison between healthy and pathological subjects demonstrated high discriminatory power (AUC=0.866; Accuracy=0.826), with optimized sensitivity of 100% and specificity of 80%, highlighting the presence of a characteristic FT-IR spectral signature associated with the AML and sarcomatous pathology (Figure 1A). The comparison between the diagnostic phase and BM relapse or myeloid sarcoma showed excellent discrimination (Figures 1B and 1C), as well as the difference between medullary and sarcomatous relapse (Figure 1D). This suggests that, although differences exist, the metabolic, molecular and structura profile detected by FT-IR differs in the two types of relapse, indicating peculiar biochemical features related with the clinical presentations. Furthermore, analysis of post-HSCT spectra revealed a gradual return to a physiological condition, with normalization observed in 88.8 and 94.4% of samples at +30 and +90 days, respectively, consistent with partial metabolic recovery.In conclusion, our findings suggest for the first time that FT-IR spectroscopy applied to plasma represents a promising liquid biopsy tool for monitoring AML and its post-HSCT relapse. The method’s ability to distinguish the diagnostic phase from relapse, the relapse types, and to capture a gradual normalization of the spectral profile in post-transplant samples (up to 94.4% of patients at 90 days) indicates that plasma biochemical changes accurately reflect the patients’ clinical and metabolic status, supporting its potential use in clinical practice.FT-IR offers a sensitive, non-invasive approach to detect biochemical alterations, potentially providing complementary biomarkers for disease monitoring and early relapse prediction. Further studies are warranted to explore these FT-IR-derived signatures and to integrate this approach into precision monitoring strategies for AML patients.
  • TRACKING RESIDUAL CML: COMPLEMENTARY INSIGHTS FROM CD26+ STEM CELLS AND EXTRACELLULAR BCR::ABL
    Alessia Cavalleri
    Haematologica, 2026
    Chronic myeloid leukemia (CML) is a myeloproliferative disorder driven by the BCR::ABL1 fusion gene. Although tyrosine kinase inhibitors (TKI) have revolutionized disease management, leukemic stem cells (LSCs) persist, sustaining minimal residual disease and relapse. A subset expressing the CD26 membrane marker represents a population of proliferating LSCs detectable in bone marrow and peripheral blood. Parallelly, extracellular vesicles (EV) have emerged as promising circulating biomarkers, as they carry BCR::ABL1 transcripts protected within their lipidic membrane. However, the relationship between residual CD26+ LSCs and EV-associated BCR::ABL1 remains unclear. This study aimed to explore the correlation between circulating CD26+ LSCs and vesicular BCR::ABL1 transcripts as complementary indicators of residual disease activity in CML.Peripheral blood (PB) from 44 adult CML patients in at least major molecular response under TKI therapy was analyzed. Circulating CD26+ LSCs were quantified by multiparametric flow cytometry on the CD45+/CD34+/CD38- population using four-color staining. The extracellular vesicle-enriched secretome (EVES) was isolated from plasma and characterized by Western blot, colloidal nanoplasmonic assay, and atomic force microscopy. Vesicular BCR::ABL1 transcripts were quantified by digital PCR (dPCR) and compared with BCR::ABL1 levels in PB cells.EVES characterization confirmed vesicular particles expressing CD63 and FLOT-1 (Figure 1A), with minimal soluble protein contamination (Figure 1B) and typical spherical morphology (Figure 1C). The median number of circulating CD26+ LSCs was 0.00625 cells/μL (range 0-0.1565), with 32% of patients showing undetectable levels. Median EVES BCR::ABL1 was 0.230 copies/μL (range 0-0.790), with 14% undetectable (Figure 1D). No correlation was found between CD26⁺ cells or EVES BCR::ABL1 and molecular response, BCR::ABL1 IS%, or dPCR values in PB cells, nor with age or therapy duration (Figure 1E). A significant inverse correlation was observed between CD26+ LSC counts and vesicular BCR::ABL1 transcripts (r = –0.39, p = 0.0085), even stronger in deep molecular responders (r = –0.45, p = 0.0079) (Figure 1F). Patients in treatment-free remission showed higher CD26+ LSC counts, whereas EVES BCR::ABL1 tended to be higher in those under TKI treatment (Figure 1G).Circulating CD26+ LSCs and vesicle-associated BCR::ABL1 transcripts show an inverse relationship, reflecting complementary aspects of residual leukemic activity in CML. As CD26+ LSCs decline, vesicular BCR::ABL1 may increase, possibly indicating activation of alternative leukemic compartments or enhanced vesicular secretion. Combined monitoring of these cellular and vesicular biomarkers may improve detection of residual disease and provide new insights into CML biology. Larger studies are needed to validate these findings and define the biological and prognostic significance of BCR::ABL1-loaded vesicles.
  • Ethanol-Guided Hybridization of Extracellular Vesicles with Liquid-Crystalline Lipid Nanoparticles
    Valentina Pacciani, Jacopo Cardellini, Arianna Balestri, Marta Rojas-Rodríguez, Martino Calamai, Mattia Tiboni, Luca Casettari, Catherine E. Saunders, Anam A. Karimi, Gennaro Sanità, Emanuela Esposito, Andrea Zendrini, Annalisa Radeghieri, Lucia Paolini, Paolo Bergese, Costanza Montis, Lucrezia Caselli, Debora Berti
    ACS Applied Materials and Interfaces, 2026
    High Resolution Image Download MS PowerPoint Slide Hybrid nanosystems that integrate biological and synthetic lipid assemblies hold great promise for tailoring nanoscale interfaces with programmable chemical and structural functionality. However, existing approaches to hybridize extracellular vesicles (EVs) with lipid nanoparticles (LNPs) compromise either the EV bioactivity or the native supramolecular organization of synthetic LNPs, undermining structure-dependent functionality. Here, we introduce an ethanol-mediated microfluidic assembly route that enables the in situ formation and hybridization of nonlamellar liquid-crystalline lipid nanoparticles (LCNPs) with red-blood-cell-derived EVs (RBCEVs) in a single step. This process exploits ethanol-induced interfacial reorganization to drive EV incorporation without compromising the LCNP cubic architecture. Synchrotron small-angle X-ray scattering (SAXS) and cryogenic electron microscopy reveal hybrid nanoparticles that retain long-range cubic order, with RBCEV membrane proteins localized within phase-segregated nanodomains. Single-particle Raman analysis and enzymatic assays confirm molecular-level hybridization and preserved EV biofunctionality. Hybrid LCNPs also exhibit enhanced uptake in HEK293t cells. Mechanistic SAXS studies uncover that ethanol transiently stabilizes a swollen sponge-like intermediate, which mediates controlled fusion and acts as a structural template upon solvent removal, imparting long-lasting structural stability. This study elucidates the physicochemical mechanism of ethanol-guided hybridization between biogenic systems and soft nanostructured colloids, establishing design principles for structurally controlled nanohybrids with broad applicability in nanomedicine.
  • Orthogonal Investigation at Single-Particle and Ensemble Levels Uncovers Lipoprotein-Extracellular Vesicle Binding
    Angelo Musicò, Roberto Frigerio, Karl Normak, Sabrina Scolari, Alessandro Gori, Paolo Arosio, Annalisa Radeghieri, Lucia Paolini, Miriam Romano, Irantzu Llarena, Sergio E. Moya, Andrea Zendrini, Paolo Bergese
    Analytical Chemistry, 2026
    Mesoscale interactions critically shape the biological identity of extracellular nanoparticles, including extracellular vesicles. These interactions encompass biomolecular coronas, transient aggregation, and fusion events. Among them, the interaction between extracellular vesicles and lipoproteins has recently garnered significant attention due to their potential impact on functionality and in vivo fate of extracellular vesicles. In this work, we present a first investigation of the binding between human red blood cell-derived extracellular vesicles and lipoproteins across multiple scales, in both buffer and plasma. Red blood cell-derived extracellular vesicles were selected as a model system for their physicochemical homogeneity, potential in personalized medicine, and production scalability. To achieve this, we employed an ad hoc suite of orthogonal analytical techniques: fluorescence cross-correlation spectroscopy (FCCS), super-resolution microscopy, flow cytometry, and Single Molecule Array assays (Simoa). Our results reveal class-specific and context-dependent extracellular vesicle-lipoprotein associations. Notably, lipoproteins bind to extracellular vesicles with affinities ranging from 10 nM to 1 μM and with up to 100% extracellular vesicles interacting with high-density lipoproteins in the presence of plasma proteins. These findings uncover a complex and dynamic interactome of red blood cell-derived extracellular vesicles across lipoprotein classes. This work establishes a robust methodological framework for studying mesoscale interactions of extracellular nanoparticles under physiologically relevant conditions. Its versatility allows for its application to diverse interaction scenarios, supporting systematic investigation of context-dependent effects on EV-LP binding.
  • Red blood cell-derived extracellular vesicles as biomaterials: the opportunity of freezing-induced accelerated aging
    Lucia Paolini, Miriam Romano, Valentina Mangolini, Selene Tassoni, Shuhan Jiang, Elena Laura Mazzoldi, Angelo Musicò, Andrea Zendrini, Anna Kashkanova, Vahid Sandoghdar, Anna C. Berardi, Silvia Clara Giliani, Paolo Bergese, Annalisa Radeghieri
    Biomaterials Science, 2026
    Freeze–thaw-induced ageing enables the generation of homogeneous RBC-EV populations with preserved bioactivity, enhancing their standardization and applicability as biomaterials in nanomedicine.
  • Tracking leukemic residuals: dissecting the inverse relationship between CD26+ stem cells and extracellular BCR::ABL1 transcript in Chronic Myeloid Leukemia (CML)
    Silvia Mutti, Alessia Cavalleri, Anna Sicuranza, Paola Pacelli, Claudia Ielo, Lucia Paolini, Valentina Mangolini, Alessandro Leoni, Teresa Miracapillo, Camilla Turriziani, Elisabetta Abruzzese, Mirko Farina, Annalisa Radeghieri, Michele Malagola, Massimo Breccia, Monica Bocchia, Domenico Russo, Simona Bernardi
    Stem Cells Translational Medicine, 2025
    Chronic myeloid leukemia (CML) persists due to leukemic stem cells, notably the CD26+ subset. We investigated correlations between circulating CD26+ leukemic stem cells (LSCs) and BCR::ABL1 transcripts in an extracellular vesicle–enriched secretome (EVES) from plasma samples of 44 CML patients. EVES were characterized and BCR::ABL1 quantified via digital PCR. We observed an inverse correlation between CD26+LSC counts and EVES BCR::ABL1 levels, especially in deep molecular responders (DMR). CD26+LSCs were elevated in patients in treatment-free remission (TFR), while EVES BCR::ABL1 levels were higher in those receiving therapy. These findings suggest distinct dynamics between LSC populations and vesicle-mediated transcript release, with potential implications for CML monitoring and prognosis.
  • Recommendations for Studying In Situ Extracellular Vesicles From Solid Tissue
    Rossella Crescitelli, Yiyao Huang, An Hendrix, Andrew F. Hill, Stephanie N. Hurwitz, Tsuneya Ikezu, Efrat Levy, Berta Puig, Lucia Paolini, Laura J. Vella
    Journal of Extracellular Vesicles, 2025
    Solid tissue‐derived extracellular vesicles (ST‐EVs) are extracellular vesicles (EVs) separated directly from solid tissues of both vertebrates and invertebrates. ST‐EVs provide a physiologically relevant snapshot of tissue‐specific molecular dynamics and can be enriched directly in situ, from tissues in their natural state, preserving the native characteristics of ST‐EVs. However, their enrichment presents unique technical challenges compared to EVs derived from biofluids or cell culture media. The need for transparent reporting in ST‐EV research is crucial to enhance the reproducibility, comparability, and reliability of research findings. The Solid Tissue Task Force, part of the Scientific Reproducibility Subcommittee of International Society for Extracellular Vesicles, aims to recommend reporting parameters and identify outstanding questions related to the pre‐analytical and analytical handling of solid tissues, as well as ST‐EV separation and characterization. These steps are essential for advancing the understanding of the biological roles of ST‐EVs and their potential clinical applications.
  • Assessment of Chronic Myeloid Leukaemia In Vitro Models Variability: Insights Into Extracellular Vesicles
    Silvia Mutti, Alessia Cavalleri, Stefania Federici, Valentina Mangolini, Lucia Paolini, Cristian Bonvicini, Rosalba Monica Ferraro, Elena Laura Mazzoldi, Luca Garuffo, Besjana Xhahysa, Alessandro Leoni, Federica Trenta, Federica Re, Silvia Clara Giliani, Daniele Avenoso, Mirko Farina, Michele Malagola, Domenico Russo, Simona Bernardi
    Journal of Cellular and Molecular Medicine, 2025
    Chronic Myeloid Leukaemia is driven by the BCR::ABL1 fusion gene. Although Tyrosine Kinase Inhibitors have significantly improved patient outcomes, drug resistance and disease persistence remain challenges, highlighting the need for effective preclinical models. We observed cellular heterogeneity among CML models in response to TKIs, influencing viability, metabolism, and molecular markers. With growing interest in extracellular vesicles as mediators of leukaemia progression via oncogenic cargo like BCR::ABL1, we explored whether EVs from different CML cell lines exhibit distinct features. EVs from K562 and KCL22 cells were characterised under basal conditions using Fourier Transform Infrared spectroscopy, Atomic Force Microscopy, dot blotting, and Nanoparticle Tracking Analysis. We assessed EV release and BCR::ABL1 content before and after treatment with imatinib, nilotinib, or dasatinib, alongside Ki67 expression to gauge proliferation. Fourier Transform Infrared Spectroscopy with Principal Component Analysis revealed distinct clustering of EVs by cell line. While untreated K562 and KCL22 cells showed similar EV output and BCR::ABL1 content, post‐treatment K562 cells released more EVs with elevated BCR::ABL1 transcripts. KCL22 cells showed earlier reduction in Ki67 expression. These findings highlight model‐dependent EV behaviour, reflecting patient heterogeneity and reinforcing the need for careful model selection in CML research.
  • The gold nanoparticle-lipid membrane synergy for nanomedical applications
    Lucrezia Caselli, Lucia Paolini, Wye-Khay Fong, Costanza Montis, Andrea Zendrini, Jacopo Cardellini, Paolo Bergese, Debora Berti
    Nanoscale Horizons, 2025
    The integration of gold nanoparticles (AuNPs) with lipid bilayers, gives rise to powerful synergistic effects arising from nanoscale interactions.
  • Nanoplasmonic Isosbestics Uncover Mesoscale Assembly of Gold Nanoparticles on Soft Templates
    Jacopo Cardellini, Ilaria De Santis, Giuseppe Emanuele Lio, Marco Brucale, Francesco Valle, Virginia Catani, Ilenia Mastrolia, Marta Calabria, Massimo Dominici, Andrea Zendrini, Annalisa Radeghieri, Lucia Paolini, Paolo Bergese, Lucrezia Caselli, Debora Berti, Costanza Montis
    Journal of the American Chemical Society, 2025
  • Helminth extracellular vesicles co-opt host monocytes to drive T cell anergy
    Anne Borup, Mohammad Farouq Sharifpour, Litten S. Rossen, Bradley Whitehead, Anders T. Boysen, Rikke Olesen, Anja B. Bohn, Andrea Ridolfi, Marco Brucale, Francesco Valle, Lucia Paolini, Annalisa Radeghieri, Paolo Bergese, Kim Miles, Margaret Veitch, Tamara Thomas, Roland Ruscher, Phurpa Wangchuk, Paul Giacomin, Alex Loukas, Peter Nejsum
    Journal of Extracellular Vesicles, 2025
  • Extracellular vesicles of different cellular origin feature distinct biomolecular corona dynamics
    Angelo Musicò, Andrea Zendrini, Santiago Gimenez Reyes, Valentina Mangolini, Lucia Paolini, Miriam Romano, Andrea Papait, Antonietta Rosa Silini, Paolo Di Gianvincenzo, Arabella Neva, Marina Cretich, Ornella Parolini, Camillo Almici, Sergio E. Moya, Annalisa Radeghieri, Paolo Bergese
    Nanoscale Horizons, 2024
  • Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
    Joshua A. Welsh, Deborah C. I. Goberdhan, Lorraine O'Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A. P. Driedonks, Uta Erdbrügger, Juan M. Falcon‐Perez, Qing‐Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic‐Talisman, Nicole A. Kruh‐Garcia, Vroniqa Ku'ulei‐Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens‐Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter‐Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J. C. van Herwijnen, Marca H. M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, Clotilde Théry, Kenneth W. Witwer, and
    Journal of Extracellular Vesicles, 2024
  • Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable”
    Angelo Musicò, Rossella Zenatelli, Miriam Romano, Andrea Zendrini, Silvia Alacqua, Selene Tassoni, Lucia Paolini, Chiara Urbinati, Marco Rusnati, Paolo Bergese, Giuseppe Pomarico, Annalisa Radeghieri
    Nanoscale Advances, 2023
  • Large-scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop
    Lucia Paolini, Marta Monguió‐Tortajada, Marta Costa, Fabio Antenucci, Mario Barilani, Marta Clos‐Sansalvador, André Cronemberger Andrade, Tom A. P. Driedonks, Sara Giancaterino, Stephanie M. Kronstadt, Rachel R. Mizenko, Muhammad Nawaz, Xabier Osteikoetxea, Carla Pereira, Surya Shrivastava, Anders Toftegaard Boysen, Simonides Immanuel van de Wakker, Martijn J. C. van Herwijnen, Xiaoqin Wang, Dionysios C. Watson, Mario Gimona, Maria Kaparakis‐Liaskos, Konstantin Konstantinov, Sai Kiang Lim, Nicole Meisner‐Kober, Michiel Stork, Peter Nejsum, Annalisa Radeghieri, Eva Rohde, Nicolas Touzet, Marca H. M. Wauben, Kenneth W. Witwer, Antonella Bongiovanni, Paolo Bergese
    Journal of Extracellular Biology, 2022
  • Comparison of separation methods for immunomodulatory extracellular vesicles from helminths
    Anne Borup, Anders T. Boysen, Andrea Ridolfi, Marco Brucale, Francesco Valle, Lucia Paolini, Paolo Bergese, Peter Nejsum
    Journal of Extracellular Biology, 2022
  • A different protein corona cloaks “true-to-life” nanoplastics with respect to synthetic polystyrene nanobeads
    Serena Ducoli, Stefania Federici, Roland Nicsanu, Andrea Zendrini, Claudio Marchesi, Lucia Paolini, Annalisa Radeghieri, Paolo Bergese, Laura E. Depero
    Environmental Science Nano, 2022
  • A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles
    Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
    Nanoscale Horizons, 2021
  • Corrigendum: Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions (Frontiers in Bioengineering and Biotechnology, (2020), 7, (452), 10.3389/fbioe.2019.00452)
    Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
    Frontiers in Bioengineering and Biotechnology, 2021
  • MicroRNA-34a-5p expression in the plasma and in its extracellular vesicle fractions in subjects with Parkinson's disease: An exploratory study
    Ilaria Grossi, Annalisa Radeghieri, Lucia Paolini, Vanessa Porrini, Andrea Pilotto, Alessandro Padovani, Alessandra Marengoni, Alessandro Barbon, Arianna Bellucci, Marina Pizzi, Alessandro Salvi, Giuseppina De Petro
    International Journal of Molecular Medicine, 2021
  • AFM-Based High-Throughput Nanomechanical Screening of Single Extracellular Vesicles
    Andrea Ridolfi, Marco Brucale, Costanza Montis, Lucrezia Caselli, Lucia Paolini, Anne Borup, Anders T. Boysen, Francesca Loria, Martijn J. C. van Herwijnen, Marije Kleinjan, Peter Nejsum, Natasa Zarovni, Marca H. M. Wauben, Debora Berti, Paolo Bergese, Francesco Valle
    Analytical Chemistry, 2020
  • Biogenic supported lipid bilayers as a tool to investigate nano-bio interfaces
    Costanza Montis, Annalisa Salvatore, Francesco Valle, Lucia Paolini, Francesco Carlà, Paolo Bergese, Debora Berti
    Journal of Colloid and Interface Science, 2020
  • Augmented COlorimetric NANoplasmonic (CONAN) Method for Grading Purity and Determine Concentration of EV Microliter Volume Solutions
    Andrea Zendrini, Lucia Paolini, Sara Busatto, Annalisa Radeghieri, Miriam Romano, Marca H. M. Wauben, Martijn J. C. van Herwijnen, Peter Nejsum, Anne Borup, Andrea Ridolfi, Costanza Montis, Paolo Bergese
    Frontiers in Bioengineering and Biotechnology, 2020
  • The nanostructured secretome
    S. Busatto, A. Zendrini, A. Radeghieri, L. Paolini, M. Romano, M. Presta, P. Bergese
    Biomaterials Science, 2020
  • Extracellular vesicles in regenerative medicine
    Miriam Romano, Andrea Zendrini, Lucia Paolini, Sara Busatto, Anna C. Berardi, Paolo Bergese, Annalisa Radeghieri
    Nanomaterials for Theranostics and Tissue Engineering Techniques Trends and Applications, 2020
  • Fourier-transform Infrared (FT-IR) spectroscopy fingerprints subpopulations of extracellular vesicles of different sizes and cellular origin
    Lucia Paolini, Stefania Federici, Giovanni Consoli, Diletta Arceri, Annalisa Radeghieri, Ivano Alessandri, Paolo Bergese
    Journal of Extracellular Vesicles, 2020
  • Analysis of a nanoparticle‑enriched fraction of plasma reveals miRNA candidates for down syndrome pathogenesis
    Alessandro Salvi, Marika Vezzoli, Sara Busatto, Lucia Paolini, Teresa Faranda, Edoardo Abeni, Maria Caracausi, Francesca Antonaros, Allison Piovesan, Chiara Locatelli, Guido Cocchi, Gualtiero Alvisi, Giuseppina De Petro, Doris Ricotta, Paolo Bergese, Annalisa Radeghieri
    International Journal of Molecular Medicine, 2019
  • Erratum: Analysis of a nanoparticle‑enriched fraction of plasma reveals miRNA candidates for down syndrome pathogenesis(Int J Mol Med (2019) 43(2303‑2318) DOI: 10.3892/ijmm.2019.4158)
    Alessandro Salvi, Marika Vezzoli, Sara Busatto, Lucia Paolini, Teresa Faranda, Edoardo Abeni, Maria Caracausi, Francesca Antonaros, Allison Piovesan, Chiara Locatelli, Guido Cocchi, Gualtiero Alvisi, Giuseppina De Petro, Doris Ricotta, Paolo Bergese, Annalisa Radeghieri
    International Journal of Molecular Medicine, 2019
  • Collapse of the plasmacytoid dendritic cell compartment in advanced cutaneous melanomas by components of the tumor cell secretome
    Raffaella Vescovi, Matilde Monti, Daniele Moratto, Lucia Paolini, Francesca Consoli, Luisa Benerini, Laura Melocchi, Stefano Calza, Mariella Chiudinelli, Giulio Rossi, Mattia Bugatti, Michele Maio, Ester Fonsatti, Camillo Farisoglio, Michele Simbolo, Camillo Almici, Rosanna Verardi, Aldo Scarpa, Paolo Bergese, Ausilia Manganoni, Fabio Facchetti, William Vermi
    Cancer Immunology Research, 2019
  • Intersectin goes nuclear: Secret life of an endocytic protein
    Gualtiero Alvisi, Lucia Paolini, Andrea Contarini, Chiara Zambarda, Veronica Di Antonio, Antonella Colosini, Nicole Mercandelli, Martina Timmoneri, Giorgio Palù, Luigi Caimi, Doris Ricotta, Annalisa Radeghieri
    Biochemical Journal, 2018
  • Biophysical properties of extracellular vesicles in diagnostics
    Lucia Paolini, Andrea Zendrini, Annalisa Radeghieri
    Biomarkers in Medicine, 2018
  • Exosomes secreted by hela cells shuttle on their surface the plasma membrane-associated sialidase NEU3
    Lucia Paolini, Flavia Orizio, Sara Busatto, Annalisa Radeghieri, Roberto Bresciani, Paolo Bergese, Eugenio Monti
    Biochemistry, 2017
  • Size distribution of extracellular vesicles by optical correlation techniques
    Costanza Montis, Andrea Zendrini, Francesco Valle, Sara Busatto, Lucia Paolini, Annalisa Radeghieri, Annalisa Salvatore, Debora Berti, Paolo Bergese
    Colloids and Surfaces B Biointerfaces, 2017
  • Quantification of β region IgA paraproteins - Should we include immunochemical "heavy/light chain" measurements? Counterpoint
    Lucia Paolini
    Clinical Chemistry and Laboratory Medicine, 2016
  • Residual matrix from different separation techniques impacts exosome biological activity
    Lucia Paolini, Andrea Zendrini, Giuseppe Di Noto, Sara Busatto, Elisabetta Lottini, Annalisa Radeghieri, Alessandra Dossi, Andrea Caneschi, Doris Ricotta, Paolo Bergese
    Scientific Reports, 2016
  • Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia
    Lucia Paolini, Giuseppe Di Noto, Francesca Maffina, Giovanni Martellosio, Annalisa Radeghieri, Caimi Luigi, Doris Ricotta
    Annals of Clinical Biochemistry, 2015
  • Polyclonal versus monoclonal immunoglobulin-free light chains quantification
    Giuseppe Di Noto, Elena Cimpoies, Alessandra Dossi, Lucia Paolini, Annalisa Radeghieri, Luigi Caimi, Doris Ricotta
    Annals of Clinical Biochemistry, 2015
  • Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles
    Daniele Maiolo, Lucia Paolini, Giuseppe Di Noto, Andrea Zendrini, Debora Berti, Paolo Bergese, Doris Ricotta
    Analytical Chemistry, 2015
  • Serum free light chain assays for monitoring response to treatment in a patient with pharmacoresistant light chain multiple myeloma
    Biochimica Clinica, 2014
  • Abatacept reduces levels of switched memory B cells, autoantibodies, and immunoglobulins in patients with rheumatoid arthritis
    Mirko Scarsi, Lucia Paolini, Doris Ricotta, Antonio Pedrini, Silvia Piantoni, Luigi Caimi, Angela Tincani, Paolo Airò
    Journal of Rheumatology, 2014
  • Immunoglobulin free light chains and GAGs mediate multiple myeloma extracellular vesicles uptake and secondary NfκB nuclear translocation
    Giuseppe Di Noto, Marco Chiarini, Lucia Paolini, Elena Laura Mazzoldi, Viviana Giustini, Annalisa Radeghieri, Luigi Caimi, Doris Ricotta
    Frontiers in Immunology, 2014
  • C-src Enriched Serum Microvesicles Are Generated in Malignant Plasma Cell Dyscrasia
    Giuseppe Di Noto, Lucia Paolini, Andrea Zendrini, Annalisa Radeghieri, Luigi Caimi, Doris Ricotta
    Plos One, 2013
  • The epsilon hinge-ear region regulates membrane localization of the AP-4 complex
    Lucia Paolini, Annalisa Radeghieri, Sara Civini, Luigi Caimi, Doris Ricotta
    Traffic, 2011