Unveiling a sustainable approach to cancer treatment: the antitumor activity of amygdalin and cell-free supernatant of Lacticaseibacillus rhamnosus Omnia Karem M. Riad, Hagar F. Forsan, Basma Abdulsamad, Heba Mohammed Refat M. Selim, Wafaa S. Khalaf Folia Microbiologica, 2025 The pharmaceutical industry increasingly prioritizes environmental sustainability across its strategies, focusing on developing eco-friendly anti-cancer treatments. This is particularly important considering many anti-cancer drugs are not fully metabolized and pollute aquatic ecosystems. Amygdalin, a plant-derived compound, and cell-free supernatants (CFS) of Lacticaseibacillus rhamnosus show promise as sustainable anti-cancer agents. This study evaluated the potential anti-cancer activity of amygdalin and L. rhamnosus CFS against MCF-7 and A549 cancer cell lines. Cell viability was assessed using the MTT assay in response to monotherapy and combination therapy treatments. The induction of apoptosis was investigated using flow cytometric analyses. The combination demonstrated a synergistic effect on MCF-7 and A549 cell lines, with a combination index (CI) value of 0.8159 ± 0.0245 and 0.6422 ± 0.0316, respectively. Further analysis using propidium iodide (PI) staining and Annexin V confirmed increased apoptosis in combination-treated cells compared to those treated with amygdalin alone. These findings suggest that the combination of amygdalin and CFS of L. rhamnosus exhibits a synergistic anti-cancer effect against MCF-7 and A549 cells. Therefore, this combination is considered sustainable, eco-friendly, and effective anti-cancer treatment. This is the first study to investigate the anti-cancer effect of amygdalin in combination with L. rhamnosus cell-free supernatant (CFS) while considering its potential for environmental sustainability. Further research in a xenograft animal model is warranted to validate these findings.
CASSAVA ADDITION FOR AMYGDALIN ENRICHMENT IN DAIRY BEVERAGES H. F. Forsan, M. M. El Abd, W. B. Elsabie, H. M. Sobhy Anbar Journal of Agricultural Sciences, 2024 The cassava plant is known for its numerous medicinal and nutritional benefits. This study used cassava to enhance the nutritional content of milk and enrich it with amygdalin (vitamin B17). Properties associated with amygdalin include anticoagulants, atherosclerotic, hepatic protective, anti-tumor, antidiabetic, antifungal, anti-inflammatory, anticancer, antibacterial, anti-aging, and antioxidants. Cassava paste was prepared by boiling its roots in water for 1 hour and subsequently mashing them. Raw cow’s milk, 5 % sugar, and 1 sachet of vanilla (1 gm) were mixed and heated at 80°C for 10 minutes. Fresh and refrigerated samples were tested for amygdalin concentrations at 6 ± 1°C. The amygdalin content in 5% cassava milk was 3.51 ± 0.01 mg/100 g and 3.52 ± 0.01 after 1 week of refrigeration (6± 1°C). Whereas, for 10% cassava milk it increased from 7.01 ± 0.01 mg/100 g when freshly prepared to 7.02 ± 0.02 after 1week’s refrigeration at 6 ± 1°C. This slight increase could be attributed to water evaporation during the one-week refrigeration.
Zebrafish as a Model for a Novel Neuroprotective Natural Product Ebele Joan Ajagun, Abraham Olufemi Asuku, Maryam Tayo Ayinla, Taoheed Adedeji Abdulsalam, Ayodeji Johnson Ajibare, Tobiloba Samuel Olajide, Yusuf Olamilekan Hamza, Kaosara Oyinola Ganiyu Zebrafish as A Model for Parkinsons Disease, 2024 Parkinson’s disease (PD) or Parkinsonism is an age-related progressive neurological disorder because of the deterioration of the nerve cells inside the substantia nigra, and it is characterized by three cardinal signs: rigidity, resting tremor, and bradykinesia. The major aim of therapy is to control the disorder’s signs and symptoms, delay the progression of the disease, and enhance the affected person’s quality of life (QoL). This can be achieved by the use of medication (drugs) and surgery. Pharmacotherapy of PD refers to utilizing drugs to replace, mimic, or potentiate the action of depleted endogenous brain’s dopamine and aims at delaying the progression of the disorder, reducing the drugs’ adverse effects, and improving the patient’s QoL. Drugs employed in the treatment of PD are broadly divided into two categories: dopaminergic system-acting drugs and cholinergic system-acting drugs. However, levodopa remains the first choice drug for the management of Parkinsonism to date. Despite the availability of drugs for the management of Parkinsonism, the progressive nature of the disorder has resulted in their failure to confer lasting therapeutic efficacy as the majority of the features of the disease become resistant to these drugs. Also, severe adverse effects with little or lack of enhancement in the patient’s QoL often accompany the use of multiple drug regimens, hence limiting the attainment of the overall goal of pharmacotherapy.
Applications of nanomaterials in medicine Hagar Fathy Forsan Sustainable Nanomaterials for Biomedical Engineering Impacts Challenges and Future Prospects, 2023 There are several opportunities for nanotechnology to improve medical research, thereby changing health care practices around the world. It is predicted that several new nanoparticles will be used with an immensely positive effect on human health. The potential uses of nanomaterials in medicine have been extensively studied. Nowadays, with the rapid growth of nanotechnology for medication and gene delivery, biological instruments, nanoelectronic biosensors, or molecular nanotechnology, nanomaterials themselves can be used as imaging agents or therapeutic drugs. Therefore, this chapter describes nanomaterial types, dimensions, chemical composition, and industrial nanomaterials. It also discusses some distinct nanomaterials like silver, gold, phosphorus, and hybrid chitosan for their biological and medical applications and future directions of nanomedicine.
SARS-CoV-2 vaccines from A to Z: A review of the current challenges J. M. Albalaty, F. H. Forsan, S. Awad, E. Elsadek, A. A. Rahman, M. E. S. M. Badawy, M. Khalfallah, E. S. El-Wakil, I. Ahmed, I. Ali, S. A. Nofal, M. R. Elsapagh, M. Gomaa, E. H. F. Bougafa, S. Mahmoud, A. Moustafa, M. ali, M. Barakat, M. A. Kandeel, E. A. A. Sebaei, G. Mahmoud Global Nest Journal, 2023
