Eichhornia crassipes (Water Hyacinth) Multifaceted Potential due to Many Therapeutic and Value-added Aspects Mamta Kumari, Anubhav Dubey, Niladry Sekhar Ghosh Journal of Natural Remedies, 2026 Background: Eichhornia crassipes, more often known as the water hyacinth, a member of the Pontederiaceae family, is a notoriously invasive macrophyte that inhabits watery habitats. Areas in the tropics and subtropics are its native habitats. Despite its pejorative reputation, water hyacinth causes major ecological issues, such as the depletion of water supplies. It serves a variety of purposes. Animal feed, bioenergy, biofertilizer, and industrial wastewater treatment are only a few of its numerous societal and economic applications. Aim: To identify research gaps and highlight the need for more extensive pharmacological investigations on this medicinal plant. Methods: We performed a literature search on ScienceDirect and PubMed databases using various keywords, including “water hyacinth”. The search was refined by applying relevant filters to obtain the most pertinent articles for this review article’s objective. Results: Alkaloids, sterols, tannins, saponins, flavonoids, phenols, and water hyacinth are just a few of the many bioactive plant components found in abundance in this plant. Many different medicinal effects have been associated with these secondary metabolites. Among its many pharmacological effects are those of an antioxidant, anti-inflammatory, antibacterial, neuroprotective, and hepatoprotective nature, among many others. Conclusion: The E. crassipes (Mart.) plant’s pharmacologically active components have been the subject of much study. It still needs to carry out an extensive investigation of the pharmacological properties of this medicinal plant. This review provides a thorough synopsis of the literature on E. crassipes, including its traditional usage, pharmacological and biological properties, and chemical composition. Major Findings: Eichhornia crassipes contains a rich spectrum of secondary metabolites, including alkaloids, sterols, tannins, saponins, flavonoids, and phenolic compounds. These phytochemicals demonstrate diverse pharmacological effects, such as antioxidant, anti-inflammatory, antibacterial, neuroprotective, and hepatoprotective activities.
Assessment of in vitro Antimicrobial and Antioxidant Activity of Cymbopogon flexuosus Anubhav Dubey, Paras Srivastava, Vikram Kumar Sahu, Sribatsa Lanchhana Dash, Arvind Kumar Research Journal of Chemistry and Environment, 2026 Cymbopogon is a member of the family Poaceae and has been explored for its phytochemicals and bioactivities. Although the antimicrobial activities of Cymbopogon flexuosus extracts have been extensively studied, comprehensive analyses are required to identify promising compounds for the treatment of antimicrobial resistance. Therefore, this study investigated the antioxidant and antimicrobial properties of Cymbopogon flexuosus. ethanolic extracts in every single organ. To explore the phytochemical constituents of the ethanolic and water leaf extract of Cymbopogon flexuosus, in vitro analyses were used to evaluate the antioxidant and antimicrobial properties of the Cymbopogon flexuosus. Ethanolic and water extracts. In addition, bioactivity was measured using cytotoxicity assays. Antioxidant assays were performed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis [3-ethylbenzothiazoline-6- sulfonic acid (ABTS). Finally, the antimicrobial activity of these extracts was evaluated against Candida albicans, Bacillus subtilis, Staphylococcus aureus and Escherichia coli using a well diffusion assay. GC–MS analysis revealed 53 metabolites. Of these, 2,5-bis(1,1-dimethylethyl)- phenol (27.87%), alpha-cadinol (26.76%) and 1,2- dimethoxy-4-(1-propenyl)-benzene (20.56%) were the predominant compounds. C. winterianus and C. nardus leaves exhibited the highest antioxidant activity against DPPH and ABTS respectively. Leaf extract exhibited the highest antimicrobial activity against E. coli and S. aureus whereas Cymbopogon flexuosus leaf extract showed the highest activity against microbes. Furthermore, computational pathway analysis predicted that antimicrobial activity mechanisms were related to antioxidant activity. Both the leaves showed great antimicrobial activity.
Transforming RNA-Based Gene Therapy with Innovative Nanocarriers for siRNA and miRNA Delivery Jitendra Singh Chaudhary, Dilip Kumar Chanchal, Kuldeep Singh, Jeetendra Kumar Gupta, Divya Jain, Anubhav Dubey, Mahima Pandey, Shahbaz Khan Current Gene Therapy, 2026 The cells have been given precise instructions proprio to the regulation of gene expression by the main genesis of Ryan-based gene therapy, which has revived cancer treatment and other disorders. The difficulty of delivering small interfering RNA (siRNA) and microRNA (miRNA) to a target cell is an enormous task and is often faced by researchers due to characteristic instabilities of these carriers and their poor uptake by the cell membrane. The new developments from nanocarrier technologies offer opportunities for better effectiveness of RNA therapy for its delivery and the effectiveness of the treatment regimen. The objective of this article is to provide an overview of the existing as well as the newest developments in nanocarrier technology, particularly as related to microRNA and small interfering RNA (siRNA) delivery. Their modes of operation and their uses in gene therapies are also examined as principles of their design. We focus on several nanocarrier technologies, which have shown proof of concept in multiple disciplines such as stability, controlled release profiles, and delivery. Lipid-based nanoparticles, polymeric systems, and hybrid nanocarriers are some of the platforms that fall under this category; however, this list is not exhaustive. We also study the idea that certain nanocarriers could have multiple functionalities, which would make it possible to improve cancer treatment by simultaneously carrying chemotherapy and genes. We aim to shed light on the future of RNA-based gene therapy by providing a thorough overview of recent research in the field. This will help us understand how novel nanocarrier technologies can tackle the delivery issues.
Advances in the Synthesis of GABAergic Modulators for Anxiety and Depression: Pathways and Potential Applications Kuldeep Singh, Pranshul Sethi, Divya Jain, Jeetendra Kumar Gupta, Omar Awad Alsaidan, Sami I. Alzarea, Anubhav Dubey, Mohammad Tabish, Rajagopalan Ramanathan Current Neuropharmacology, 2026 Anxiety and depression are among the most common and debilitating neuropsychiatric disorders worldwide. The shortcomings of existing treatments, including delayed onset, partial response, and unfavorable side effects, underscore the pressing need for more efficient and focused treatments. The brain's main inhibitory network, the GABAergic system, is increasingly being shown to be a crucial modulator of emotional and cognitive control. This study provides a comprehensive analysis of GABAergic modulators across pharmacological, molecular, and clinical domains in treating anxiety and depression. In contrast to past reviews, we investigate how GABA-A receptor subtypes differ in their involvement in anxiety disorders and depression subtypes (such as major depressive disorder, treatment-resistant depression, and postpartum depression), establishing links between receptor-selective pharmacology and symptom-specific treatment outcomes. We review current and post-2021 clinical studies and discuss recent developments in the synthesis of subtype- selective modulators, the function of neurosteroids, and the importance of extrasynaptic GABAergic transmission. The study also emphasizes how receptor mapping and structure-activity correlations influence the development of next-generation drugs. We propose that the development of subtype-selective GABA-A receptor modulators represents a pivotal advancement in addressing the limitations of conventional therapies, particularly their dependence, sedation, and delayed onset. By integrating medicinal chemistry with clinical neuropharmacology, this review highlights how targeting specific receptor subtypes opens the door to safer, faster-acting, and more durable treatments for anxiety and depression.
Enhancement of Omega-3 Fatty Acid Production by Metabolic Engineering or Genetic Modification of Microorganisms Chandan Mukherjee, Mohit Maurya, Deepika Shukla, Anubhav Dubey, Sanyogita Shahi, Mamta Kumari Journal of Pure and Applied Microbiology, 2025 The use of Omega-3 fatty acids is garnering increasing interest due to its substantial effects on human health. Fish are a major dietary source of ω3FA; however, they are not the primary producers. The primary producers are microalgae and other microorganisms, which serve as the original source of omega-3s in the food chain. The marine fish, however, has not been able to meet the global demands. Recently, the generation of fatty acids has been investigated from another source, namely microorganisms. Omega-3 fatty acids are naturally produced by the microorganisms. However, the use of metabolic engineering has provided evidence for improvements in production. The current article reviews research on microorganisms using engineering ways to accumulate ω3FA such as docosahexaenoic acid or DHA and eicosapentaenoic acid or EPA. These studies have demonstrated that modulation of existing pathways, as well as reconstitution of biosynthetic pathways, has a high potential for increasing the fatty acid yield. However, certain bottlenecks limit the yield of fatty acids in various host organisms. These may include the fatty acid flux of intermediates that exists between various lipid pools. Even though the heterologous and native microbes show fatty acid flux under acyltransferases control, there is evidence that modulation of even a single acyltransferase by genetic approach can provide significant alteration for producing fatty acids. The microbes with oleaginous properties are being identified rigorously and are expected to further advance the process of engineering which leads to enhanced production of fatty acids in microbes.
Development of EST-SSR Markers Based on Transcriptome Sequencing in Kodo millet (Paspalum scrobiculatum l.) Humbal Nehali Bhagavanjibhair, Dhawale Ramesh Narayanrao, M. V. Dhuppe, Rukam. S. Tomar, Mukesh Soni, Vijay Upadhye, Anubhav Dubey, Sunil Tulshiram Hajare Cytology and Genetics, 2025 Abstract Kodo millet (Paspalum scrobiculatum L.) (2n = 4x = 40) mostly found in dry and semi-arid parts of Africa and Asia, also known as miraculous millets, is a staple food and a nutrient-security crop. It’s commonly known that kodo millet has the strongest tolerance to drought. Little study was done on high throughout genomic studies in relation to the creation of millets improved by genomics. In the present investigation, 21 080 571 total reads of sequence data were included in the 6.55 gigabytes of raw sequence that was produced following the genome sequencing run. After utilizing FastQC to verify the raw data quality, a total sequence of 1 087 611 and 578 123 572 bp was obtained. Three RNA samples from mature, blooming, and vegetative specimens were gathered, and the high-throughput Ion torrent sequencing method was applied to the sequencing process. In the vegetative stage, there were 1, 34, 40, and 824 raw readings; in the reproductive and mature stages, there were 1, 66, 35, 843 reads and 97, 43, 629 bp reads, respectively. At three distinct stages, the average read length was 131.6, 137.6, and 148.8 bp, in that order. The interface modules BatchPrimer3 (version 1.0) were used to construct the SSR and EST-SSR primers. Four FASTA files including contigs from the transcriptome and genome were utilized to identify SSRs. A total of thirty SSRs were found, with GC% and Tm ranges of 40–70 and 58–62°C, respectively. Of the 30 SSR and EST-SSR primers that were used for validation, only 17 were able to amplify the DNA from 27 distinct types of Kodo millet. 24 bands were obtained from the PCR amplification of 27 distinct types. We think that these findings may be applied in the future as possible candidates to enhance this significant staple crop.
Hypolipidemic Potentials of Methanolic Extract of Cymbopogon flexuosus Anubhav Dubey, Sanyogita Shahi, Toyaj Shukla, Neetu Verma, Shubhyanka Singh, Niladry Sekhar Ghosh Journal of Natural Remedies, 2025 Background: Hyperlipidemia is a condition marked by high levels of Total Cholesterol (TC), Low-Density Lipoprotein (LDL), and Very Low-Density Lipoprotein (VLDL), along with reduced levels of High-Density Lipoprotein (HDL) in the blood. Aim: This study aimed to evaluate the cholesterol-lowering (hypolipidemic) effect of methanolic extract from Cymbopogon flexuosus leaves in rats fed a high-cholesterol diet. Methods: The anti-hyperlipidemic effects of the extract were tested in rats over 45 days. Antioxidant activity was also assessed using DPPH and hydrogen peroxide free radical scavenging assays. Results: When cholesterol was given to rats for 45 days, the activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase significantly increased, and the amounts of lipids in the tissues and plasma increased while HDL cholesterol dropped. Additionally, it lowered superoxide dismutase activity in the tissues and increased malondialdehyde levels. Co-administration of Cymbopogon flexuosus extract at doses of 100 and 200 mg/kg, however, dramatically restored lipid levels to nearly normal in rats fed high cholesterol. HDL cholesterol levels increased by 225.44% and 110.39%, while plasma, liver, heart, kidney, and lung cholesterol decreased by 23.13% and 56.43%, 30.09% and 20.90%, 38.21% and 74.53%, 12.61% and 32.49%, and 37.11% and 29.90%, respectively. While SOD activity in the liver, heart, kidney, and lung increased by 89.35% and 149.21%, 74.91% and 68.35%, 56.76% and 114.77%, and 204.91% and 274.62%, respectively, the levels of AST, ALT, and ALP in the plasma and MDA in the tissues similarly reduced. Conclusions: This study’s findings corroborate previous research showing that Cymbopogon flexuosus can reduce lipid levels in rats given a high-cholesterol diet. Major Finding: The study found that Cymbopogon flexuosus (lemongrass) can help lower fat levels in the blood and also acts as an antioxidant. These results suggest that the plant could be a promising natural treatment for high cholesterol.
Pond Aquaculture with Caged Zebrafish Fish: Review of Feeding Practices, Environmental Effects, and System Performance Dubey et al. Egyptian Journal of Aquatic Biology and Fisheries, 2025 Pond-based aquaculture incorporating caged zebrafish (Danio rerio) offers a controlled approach to managing nutrition, environmental exposure, and production outcomes while benefiting from the natural ecological buffering of pond systems. Feeding practices in caged systems must balance nutrient provision with water quality concerns, as uneaten feed rapidly accumulates within confined spaces. Optimal regimes typically use high-quality micro-pelleted diets with 40–50% protein and appropriate particle sizes, delivered in multiple small feedings to ensure efficient consumption. The surrounding pond environment influences dissolved oxygen, temperature stability, and microbial communities, which indirectly affect zebrafish metabolism and health. However, fluctuating pond conditions—such as algal blooms or seasonal temperature shifts—can stress caged fish if not monitored closely.
Comparative Study of Anti-Diabetic Effects of Nigella sativa and Asparagus racemosus Seed Extracts on Zebrafish D Tripathi, B Mishra, S Sahu, A Katiyar, SL Dash, A Dubey Egyptian Journal of Aquatic Biology and Fisheries 30 (2), 1697-1706 , 2026 2026
Isolation and Characterization of Novel Bioactive Compounds from Underexplored Aquatic Macrophytes S Shahi, A Dubey, Y Kumar, P Gupta, A Dwivedi, P Chaturvedi, S Dwivedi Egyptian Journal of Aquatic Biology and Fisheries 30 (2), 1037-1046 , 2026 2026
Rauwolfia Serpentina: Phytochemistry, Mechanisms of Action, and Clinical Implications–A Comprehensive Review A Dubey, VK Patel, VK Sahu, SL Dash, A Mishra International Journal of Pharmacy Research & Technology (IJPRT) 16 (1), 184-197 , 2026 2026 Citations: 1
Advances in the Synthesis of GABAergic Modulators for Anxiety and Depression: Pathways and Potential Applications K Singh, P Sethi, D Jain, JK Gupta, OA Alsaidan, SI Alzarea, A Dubey, ... Current Neuropharmacology , 2026 2026
A Systematic Review of Vaccination, Prevention, and Public Health Measures for the Human Papillomavirus Epidemic Among Patients in India S Obbalareddy, A Dubey, M Kumari, C Mukherjee, S Shahi, S Dutta 2026
Enhancement of Omega-3 Fatty Acid Production by Metabolic Engineering or Genetic Modification of Microorganisms. C Mukherjee, M Maurya, D Shukla, A Dubey, S Shahi, M Kumari Journal of Pure & Applied Microbiology 19 (4) , 2025 2025
Development of EST-SSR Markers Based on Transcriptome Sequencing in Kodo millet ( Paspalum scrobiculatum l.) HN Bhagavanjibhair, DR Narayanrao, MV Dhuppe, RS Tomar, M Soni, ... Cytology and Genetics 59 (6), 651-667 , 2025 2025
Pharmaceutical Delivery Methods Using Nanotechnology: Current Trends and Prospects SL Dash, S Sahu, D Tripathy, A Katiyar, S Choudhary, P Yadav, A Awasthi, ... Vascular and Endovascular Review 8 (1s), 170-182 , 2025 2025
Trace Contaminants in Drinking Water and Neurological Health M Kumari, A Dubey, KS Samanthula, S Obbalareddy, SSD Manyam, ... FishTaxa-Journal of Fish Taxonomy 36 (1s), 15-23 , 2025 2025 Citations: 1
Polysaccharide-Based Nanocarriers for Oral Delivery of Proteins and Peptides: A Paradigm Shift in Biopharmaceutical Transport S Shahi, A Dubey, C Mukherjee, VK Yadav, KS Samanthula, N Verma, ... Vascular and Endovascular Review 8 (1s), 53-59 , 2025 2025 Citations: 1
New Product Development, Evaluation, With Optimization Diclofenac Transport For Gout KS Samanthula, S Singh, M Kumari, P Mishra, AK Gupta, A Dubey, ... Vascular and Endovascular Review 8 (1s), 60-68 , 2025 2025
Zebrafish-Based Evaluation of Cymbopogon flexuosus Extract in Acrylamide-Induced Neurodegeneration. M Kumari, A Dubey, AA Sethy, A Srivastava, U Kumar, G Mishra, A Mishra, ... Egyptian Journal of Aquatic Biology & Fisheries 29 (5) , 2025 2025 Citations: 2
The Zebrafish Model in Neurological Research: Implications for Clinical Practice. A Dubey, M Kumari, NS Ghosh, RO Nyarko, JA Sackey, EA Boateng, ... Egyptian Journal of Aquatic Biology & Fisheries 29 (5) , 2025 2025
Containers on the micro-and nanoscale for neurodegenerative diseases: nose-to-brain drug transportation D Anubhav, S Sanyogita, SS Kumar, K Mamta, D Sumeet Research Journal of Biotechnology Vol 20, 8 , 2025 2025
TruthSphere: A Framework for Automated Fake News Detection in Digital Media AS Thakur, K Rai, A Verma, A Dubey 2025 International Conference on Engineering Innovations and Technologies … , 2025 2025
Advances in viral vector-based delivery systems for gene therapy: a comprehensive review K Singh, D Jain, P Sethi, JK Gupta, A Dubey, A Al Noman, S Lal, ... 3 Biotech 15 (7), 196 , 2025 2025 Citations: 10
Dynamic analysis of the transcriptome offers molecular insights into flower development in Arachis hypogaea ssp. Virginia bunch N Umretiya, A Bharose, H Jasani, B Golakiya, A Dubey, ST Hajare The Nucleus, 1-21 , 2025 2025 Citations: 4
Transforming RNA-Based Gene Therapy with Innovative Nanocarriers for siRNA and miRNA Delivery JS Chaudhary, DK Chanchal, K Singh, JK Gupta, D Jain, A Dubey, ... Current Gene Therapy , 2025 2025
QUALITATIVE PHYTOCHEMICAL SCREENING AND QUANTITATIVE ESTIMATION OF BIOACTIVE COMPOUNDS IN THE ETHANOLIC EXTRACT OF TRIBULUS TERRESTRIS L. JS Chaudhary, DK Chanchal, A Dubey, V Tomar, R Verma, M Pandey, ... Biochemical & Cellular Archives 25 (1) , 2025 2025
Improving pneumonia diagnosis correctness with the synergistic approach using swin transformer and deep learning architectures on chest X-ray images PP Singh, M Kumari, A Dubey, R Mishra, P Chhabra, S Kumar CyberMedics, 73-86 , 2025 2025 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
A review on diabetes mellitus: type1 & Type2 R Kumar, P Saha, Y Kumar, S Sahana, A Dubey, O Prakash World Journal of Pharmacy and Pharmaceutical Sciences 9 (10), 838-850 , 2020 2020 Citations: 256
Ethosomes: A novel approach in transdermal drug delivery system. N Kumar, A Dubey, A Mishra, P Tiwari International journal of pharmacy & life sciences 11 (5) , 2020 2020 Citations: 65
Investigation of proapoptotic potential of ipomoea carnea leaf extract on breast cancer cell line A Dubey, P Yadav, P Verma, R Kumar Journal of Drug Delivery and Therapeutics 12 (1), 51-55 , 2022 2022 Citations: 63
Effect of covid-19 in management of lung cancer disease: A review P Saha, RO Nyarko, P Lokare, I Kahwa, PO Boateng, C Asum Asian Journal of Pharmaceutical Research and Development 10 (3), 58-64 , 2022 2022 Citations: 47
Zebrafish as an emerging model: an important testing platform for biomedical science A Dubey, NS Ghosh, R Singh J Pharm Negat Results 13 (3), 2 , 2022 2022 Citations: 46
Review on Vinpocetine. A Dubey, N Kumar, A Mishra, Y Singh, M Tiwari International Journal of Pharmacy & Life Sciences 11 (5) , 2020 2020 Citations: 45
Techniques for increasing solubility: A review of conventional and new strategies K Yadav, AK Sachan, S Kumar, A Dubey Asian Journal of Pharmaceutical Research and Development 10 (2), 144-153 , 2022 2022 Citations: 42
INVESTIGATION OF ANTI-PYRETIC ACTIVITY OF VINPOCETINE IN WISTAR RAT ANUBHAV DUBEY*, MAMTA TIWARI, YATENDRA SINGH, NEERAJ KUMAR International Journal of Pharmaceutical Research 12 (2), 1901-1906 , 2020 2020 Citations: 40
A comprehensive review on anti-cancer properties of Amaranthus viridis A Kumar, A Katiyar, V Gautam, R Singh, A Dubey prevent 25, 27 , 2022 2022 Citations: 38
D-Pinitol-A Natural Phytomolecule and its Pharmacological effect. K Srivastava, M Tiwari, A Dubey, A Dubey International Journal of Pharmacy & Life Sciences 11 (5) , 2020 2020 Citations: 34
Queen of all herbs (Asparagus racemosus): an assessment of its botany, conventional utilization, phytochemistry and pharmacology D Anubhav, B Mrinmoy, D Biplab, G Niladry Research Journal of Biotechnology Vol 18, 6 , 2023 2023 Citations: 33
A review on current epidemiology and molecular studies of lumpy skin disease virus-an emerging worldwide threat to domestic animals A Dubey, NS Ghosh, A Gupta, S Singh J. Med. Pharm. Allied Sci 12 (1), 5635-5643 , 2023 2023 Citations: 32
Phytochemical investication and heptoprotective evalution acacia rubica extract isonized and paracetamol indused animal toxicity R Kumar, A Dubey Turkish Journal of Physiotherapy and Rehabilitation 32 (3), 65-69 , 2020 2020 Citations: 32
Recent advances for commedonal acne treatment by employing lipid nanocarriers topically S Patnaik, D Purohit, P Biswasroy, WM Diab, A Dubey International Journal of Health Sciences 6 (S8), 180-205 , 2022 2022 Citations: 31
Effect of isoproterenol and thyroxine in herbal drug used as cardiac hypertrophy. A Raj, S Tyagi, R Kumar, A Dubey, AC Hourasia 2021 Citations: 31
Herbs Derived Bioactive Compounds and their Potential for the Treatment of Neurological Disorders. A Dubey, NS Ghosh, N Agnihotri, A Kumar, M Pandey, S Nishad Clinical Schizophrenia & Related Psychoses 16 (2) , 2022 2022 Citations: 29
A discussion on vinca plant A Dubey, D Tiwari, K Srivastava, O Prakash, R Kushwaha Journal of Pharmacognosy and Phytochemistry 9 (5), 27-31 , 2020 2020 Citations: 28
Investigation on anti-ulcer activity of Momordica dioica fruits in Wistar Rat A Kumar, A Dubey, R Singh International Journal for Research in Applied Sciences and Biotechnology 9 … , 2022 2022 Citations: 27
Medicinal properties of Cinchona alkaloids-A brief review A Dubey, Y Singh Asian Journal of Research in Pharmaceutical Sciences 11 (3), 224-8 , 2021 2021 Citations: 27
An in-depth and in vitro evaluation of the antioxidant and neuroprotective activity of aqueous and ethanolic extract of Asparagus racemosus Linn seed D Anubhav, G Niladry, S Ranjit Research Journal of Chemistry and Environment 27, 46-66 , 2023 2023 Citations: 26