Ibrahim Elsayed Mohamed Helal

@king abdulaziz university

Department of Agriculture, Faculty of Environment Sciences
Ibrahim E. Helal

Ibrahim Elsayed Mohamed Helal


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https://researchid.co/ibrahimhelal

RESEARCH, TEACHING, or OTHER INTERESTS

General Veterinary, Equine, Food Animals, Small Animals
15

Scopus Publications

Scopus Publications

  • Green Coffee Extract Mitigates Fipronil-Induced Endocrine Disruption, Metabolic Disturbances and Oxidative Stress in Male Albino Rats
    Alaa Hlail Dahham, Mohamed Korish, Samir Mohamed El Rayes, Nadia A. El-Fahla, Ibrahim E. Helal, Heba M. A. Abdelrazek
    Toxics, 2026
    This study evaluated the protective effects of green coffee (Coffea arabica L.) extract (GCE) against metabolic and endocrine disturbances induced by fipronil (FIP) in male rats. Animals were randomly allocated into four groups (n = 6): control, GCE (100 mg/kg), FIP (4.85 mg/kg), and combined FIP + GCE, and treated orally for 90 days. FIP exposure significantly impaired glucose homeostasis, as indicated by a 14.8% increase in the oral glucose tolerance test (OGTT) response and a 2.4-fold increase in the homeostatic model assessment of insulin resistance (HOMA-IR). It also disrupted lipid metabolism, with marked elevations in triglycerides (74.10%) and total cholesterol (57.55%). Endocrine imbalance was evident, including increased resistin levels (113.86%) and reduced triiodothyronine (T3; −37.5%), adiponectin (−42.73%), and high-density lipoprotein (HDL; −9.31%). Oxidative stress and inflammation were significantly enhanced, as demonstrated by elevated malondialdehyde (MDA; +93.56%) and pro-inflammatory cytokines (IL-1β: +246.56%; IL-6: +275%), alongside a reduction in total antioxidant capacity (TAC; −45.24%). Additionally, serum albumin levels decreased markedly (−54%). Co-administration of GCE significantly improved metabolic, hormonal, and inflammatory parameters, including insulin resistance (HOMA-IR). Histopathological analysis further confirmed its protective effects on hepatic and renal tissues. Overall, GCE mitigates FIP-induced metabolic and endocrine dysfunction, likely through its antioxidant and anti-inflammatory properties.
  • Detergent-Based Decellularization Preserves Extracellular Matrix Ultrastructure in Ovine Soft Tissues
    Ibrahim E. Helal, Mahmoud F. Ahmed, Ahmed M. Abdellatif, Mohamed A. Hashem, Hatim A. Al-Abbadi, Elsayed Metwally
    Biomimetics, 2026
    Decellularized extracellular matrix (dECM) scaffolds derived from xenogeneic tissues represent promising biomaterials for tissue engineering. In this study, dECM scaffolds were developed and characterized from four ovine tissues—skin, tunica vaginalis, fascia lata, and pericardium—using a detergent-based decellularization protocol to evaluate decellularization efficiency and extracellular matrix (ECM) preservation. Decellularization was performed using a sequential detergent-based protocol with sodium dodecyl sulfate and Triton X-100. Decellularization efficacy and matrix preservation were evaluated through gross examination, histological analysis, scanning electron microscopy (SEM), and residual DNA quantification. Gross inspection revealed increased translucency and reduced pigmentation in decellularized tissues compared with native counterparts, indicating effective cellular removal while maintaining overall tissue architecture. Histological assessment confirmed the complete absence of nuclear and cytoplasmic material, alongside preservation of collagen-rich extracellular matrix organization. SEM analysis demonstrated well-maintained ultrastructural features, including aligned collagen fibers and porous ECM architecture, with complete removal of epithelial and stromal cellular elements. Quantitative analysis revealed approximately 94% reduction in residual DNA content across all decellularized tissues compared with native controls. This study demonstrated that the employed detergent-based protocol reliably produces structurally preserved, acellular scaffolds from multiple ovine tissues. The resulting biomaterials exhibit structural characteristics that support their potential use in tissue engineering applications, pending further functional validation.
  • GUT DYSBIOSIS: CAUSES AND PREVENTION STRATEGIES – A REVIEW
    Youssef A. Attia, Ibrahim E. Helal, Sameer A. Nagadi, Asmaa F. Khafaga, Ayman E. Taha, Khalid A. Asiry, Nisreen M. Abdulsalam, Nidal M. Zabermawi, Manal E. Shafi, Hana A. Zakaria, Tarek A. Ebeid, Ibrahim H. Al-Homidan, Fulvia Bovera, Vincenzo Tufarelli
    Annals of Animal Science, 2025
    Gut dysbiosis in poultry presents a major challenge, requiring a comprehensive approach to sustain optimal gut health and ensure animal welfare. This review emphasizes the importance of integrated management practices and explores alternatives to antibiotics, including probiotics, prebiotics, synbiotics, postbiotics, phytogenic compounds, medicinal plants, and organic acids. Additionally, it advocates tailored nutritional strategies specific to broiler strains and highlights the potential of advanced biotechnologies for poultry nutrition and health management. Maintaining effective gut health is essential not only for animal welfare but also for the economic sustainability of poultry farming. Given the implications of antibiotic use in poultry for human health, the “One Health” approach is critical for recognizing the interconnection between animal, human, and environmental health. Research should prioritize understanding host-microbiome interactions, developing innovative therapies, and enhancing diagnostic tools for early detection and intervention in gut dysbiosis. Addressing this complex issue requires collaboration among researchers, veterinarians, feed manufacturers, and poultry producers to create sustainable solutions that improve animal health and production efficiency, while reducing reliance on antibiotics. Implementing a holistic strategy that encompasses various aspects of poultry management. The industry can mitigate the adverse effects of gut dysbiosis and promote the overall health and welfare of broiler chickens.
  • Modulating Effects of L-Arginine and Tribulus terrestris Extract on Fipronil-Induced Interference in the Male Reproductive System of Rats: Antioxidant Potential, Androgen Receptors, and Nitric Oxide Synthase Interplay
    Doaa H. Elsayed, Ahmed A. Bakhashwain, Eman A. Ahmed, Hatim A. Al-Abbadi, Heba M. A. Abdelrazek, Menna Allah I. El-Menyawy, Wafaa K. Teleb, Noran M. Tawfik, Ibrahim E. Helal, Heba N. Gad EL-Hak
    Toxics, 2025
    The protective potentials of Tribulus terrestris (TT) and L-arginine (L-Arg) against reproductive toxicity induced by fipronil (FPN) in male rats were investigated. A total of 36 male rats were allocated into six groups: control, TT, L-Arg, FPN, FPN + TT, and FPN + L-Arg groups. The body and sex organ weights, semen criteria, serum testosterone levels, and testicular oxidative stress were determined. Sexual behavior, testicular and penile androgen receptor (AR), penile nitric oxide synthase (NOS), immunohistochemistry of proliferating cell nuclear antigen (PCNA), and histopathology were also assessed. FPN disrupted reproductive health by influencing the expression and activity of NOS and AR, leading to compromised erectile function, sexual dysfunction, and hormonal imbalance. Significant improvements in body weight, reproductive organ weights, the expression of NOS and AR, and testosterone levels were observed in the TT- and L-Arg-treated groups. Behavioral assessments indicated improved sexual performance in the TT- and L-Arg-treated groups. Histopathological studies of the testes and penis tissue, immunohistochemical expression of PCNA in testicular tissues, and biochemical analyses further confirmed the protective effects of TT and L-Arg. Collectively, these findings highlighted the potential of TT and L-Arg in counteracting FPN-induced reproductive impairments.
  • Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways
    Doaa H. Elsayed, Sameer A. Nagadi, Heba M.A. Abdelrazek, Ahmed. S. El‑Hawy, Moharram F. El-Bassiony, Seham A. Helmy, Yasmina K. Mahmoud, Ibrahim E. Helal, Shady T. Hassan
    Ecotoxicology and Environmental Safety, 2025
    The study aimed to explore the protective effect of Nannochloropsis oculata (N.oculata) on lead induced reproductive toxicity in rams. Sixteen Barki rams were divided equally in to 4 groups; group A: control, group B: was administered 3 % dietary N. oculate, group C: was gavaged 5 mg/kg/day lead acetate and group D: was supplemented with 3 % dietary N. oculata and gavaged with 5 mg/kg/day lead acetate. The experiment lasted 180 days. Semen and blood samples were obtained. Ejaculates were examined for semen criteria besides, analysis of testosterone. Testicular oxidant/antioxidant markers, cytokines, gene expression of interleukin-6 (IL-6) and nuclear factor Kappa B (NF-κB) were estimated. Alga-treated rams revealed significant upgrades in semen criteria, serum testosterone and reduced glutathione (GSH); meanwhile, downregulation in malondialdehyde (MDA), IL-4, IL-2 as well as gene expressions of IL-6 and NF-κB as compared to other treated groups. However, lead-treated rams showed significant deteriorations in semen criteria and reduced GSH while significant elevations in MDA, IL-4, IL-2 as well as gene expression of IL-6 and NF-κB were noticed than control. Alga supplementation to lead-intoxicated rams significantly upgraded the lead induced alterations that were manifested by improvement in testicular histopathology. In conclusion, the addition of alga mitigated lead induced reproductive toxicity in rams via improving testosterone, oxidant/antioxidant status, semen criteria and reducing inflammatory cytokines.
  • Development of a novel intramuscular liposomal injection for advanced meloxicam delivery: Preparation, characterization, in vivo pharmacokinetics, pharmacodynamics, and pain assessment in an orthopedic pain model
    Pierre A. Hanna, Hatim A. Al-Abbadi, Mohamed A. Hashem, Aziza E. Mostafa, Yasmina K. Mahmoud, Eman A. Ahmed, Ibrahim M. Hegab, Ibrahim E. Helal, Mahmoud F. Ahmed
    International Journal of Pharmaceutics X, 2024
    Pain produces several physiological, and degenerative complications. This study aimed to formulate meloxicam (MLX) in liposomes to increase solubility and deliver MLX in a controlled manner to overcome its poor aqueous solubility and relatively short t 1/2 problems. Liposomes were prepared by thin film hydration followed by ultrasonication. Tests for characterizing formulations included particle size, span, entrapment efficiency, drug loading, stability, differential scanning calorimetry (DSC), Fourier transformation infrared (FT-IR) spectroscopy, morphology, in vitro release, release kinetics mathematical modeling, and an in vivo pain model in dogs undergoing orthopedic surgeries, followed by in vivo pharmacokinetics, pharmacodynamics, and pain assessment studies in comparison to the reference standard, Mobitil®. Liposomal MLX had a particle size of around 100 nm, 82 % entrapment efficiency, and 4.62 % drug loading. Stability studies, DSC, and FT-IR spectroscopy indicated that liposomes were highly stable. The formulation showed an improved in vitro controlled release pattern and an enhanced in vivo pharmacokinetic behavior as manifested by higher t 1/2 and AUC 0 – 24 and lower Cl/F in comparison to Mobitil®. The pharmacodynamics study and pain scales demonstrated liposomal MLX managed postoperative pain better than Mobitil®. In conclusion, the incorporation of MLX in liposomes increased its solubility and stability, as well as its pain management properties.
  • Analgesic Effect of Dexmedetomidine-Nalbuphine Combination vs. Dexmedetomidine Alone in Donkeys Undergoing Field Castration under Total Intravenous Anesthesia
    Ibrahim E. Helal, Hatim A. Al-Abbadi, Mohamed A. Hashem, Heba M. A. Abdelrazek, Mohammed H. Shekidef, Mahmoud F. Ahmed
    Animals, 2024
    This study evaluated the antinociceptive effect of dexmedetomidine-nalbuphine vs. dexmedetomidine alone in jacks undergoing field castration under total intravenous anesthesia. Jacks were premedicated with intravenous (IV) dexmedetomidine (5 µg/kg), either alone (Group D, n = 6) or in combination with 0.3 mg/kg nalbuphine (Group DN, n = 6). IV ketamine (1.5 mg/kg) and propofol (0.5 mg/kg) were used to induce general anesthesia, which was maintained by a continuous propofol (0.2 mg/kg/min) IV infusion. The quality of anesthesia, analgesia, and recovery were evaluated. A simple descriptive scale (SDS) was used to measure pain from the recovery time to 6 h later. The DN group exhibited improvements in analgesic and recovery quality and SDS of pain at 1-, 2-, and 3-h post-recovery. There was an apparent improvement in cardiac status, as evidenced by the enhanced heart rate and electrocardiogram findings compared to group D during surgery and recovery time. The DN group had a lower level of inflammatory cytokines, both during the surgery and shortly after recovery. Therefore, the dexmedetomidine-nalbuphine combination prior to IV anesthesia of ketamine and propofol in jacks undergoing field castration resulted in a stable surgical plane of anesthesia, improved antinociception, less pain postoperatively, and better cardiac stability.
  • Reconstruction of a partial esophageal defect using tunica vaginalis and buccal mucosa autograft: an experimental study in mongrel dogs
    Mohamed A HASHEM, Elsayed METWALLY, Yasmina K MAHMOUD, Ibrahim E HELAL, Mahmoud F AHMED
    Journal of Veterinary Medical Science, 2023
    In veterinary clinics, esophageal reconstruction is essential in many clinical situations. In this study, two autografts, the tunica vaginalis (TV) and the buccal mucosa (BM), were proposed to reconstruct a semi-circumferential cervical esophageal defect in dogs. This study aimed to verify whether these two grafts could successfully patch esophageal defects. Twelve male mongrel dogs were divided into two groups. Following cervical esophagoplasty, the defective area was patched with either a TV or a BM graft. Comprehensive clinical, serum biochemical, and histological analyses were performed to evaluate the two grafts. Throughout the study (120 days), the dogs survived the procedure well with minor complications. The lumen of the patched areas was covered with mucosa, with slight scar retraction. Compared with that of the natural esophagus, the average relative luminal diameter was not significantly decreased. Importantly, the measured cortisol and inflammatory marker levels returned to the preoperative levels after 14 days. Although histological examination revealed that both grafts repaired the esophageal defect with complete re-epithelialization, the BM graft showed a histological structure similar to that of the natural esophagus. Both grafts effectively repaired the esophageal defect with minor complications; therefore, both are recommended as promising low-cost clinical alternatives for cervical esophagoplasty in dogs.
  • Selective Calpain Inhibition Improves Functional and Histopathological Outcomes in a Canine Spinal Cord Injury Model
    Elsayed Metwally, Hatim A. Al-Abbadi, Mohamed A. Hashem, Yasmina K. Mahmoud, Eman A. Ahmed, Ahmed I. Maaty, Ibrahim E. Helal, Mahmoud F. Ahmed
    International Journal of Molecular Sciences, 2022
    Calpain activation has been implicated in various pathologies, including neurodegeneration. Thus, calpain inhibition could effectively prevent spinal cord injury (SCI) associated with neurodegeneration. In the current study, a dog SCI model was used to evaluate the therapeutic potential of a selective calpain inhibitor (PD150606) in combination with methylprednisolone sodium succinate (MPSS) as an anti-inflammatory drug. SCI was experimentally induced in sixteen mongrel dogs through an epidural balloon compression technique. The dogs were allocated randomly into four groups: control, MPSS, PD150606, and MPSS+PD150606. Clinical evaluation, serum biochemical, somatosensory evoked potentials, histopathological, and immunoblotting analyses were performed to assess treated dogs during the study. The current findings revealed that the combined administration of MPSS+PD150606 demonstrated considerably lower neuronal loss and microglial cell infiltration than the other groups, with a significant improvement in the locomotor score. The increased levels of inflammatory markers (GFAP and CD11) and calcium-binding proteins (Iba1 and S100) were significantly reduced in the combination group and to a lesser extent in MPSS or PD150606 treatment alone. Interestingly, the combined treatment effectively inhibited the calpain-induced cleavage of p35, limited cdk5 activation, and inhibited tau phosphorylation. These results suggest that early MPSS+PD150606 therapy after acute SCI may prevent subsequent neurodegeneration via calpain inhibition.
  • Enhancement of chronic wound healing with maltodextrin/ascorbic acid gel: a clinical evaluation of distal limb wounds in horses
    Ibrahim E. Helal, Hatim A. Al-Abbadi, Mohamed H. El-Daharawy, Mahmoud F. Ahmed
    Journal of Animal Science and Technology, 2022
    Delayed healing associated with distal limb wounds is highly challenging in equine clinical practice. This study aimed to evaluate healing rates between chronic non-granulating wounds of horse distal limbs that were treated with maltodextrin/ascorbic acid gel alone or in combination with povidone-iodine 1% solution and those treated with povidone-iodine 1% only throughout the study period (35 days) in clinical settings. The study was conducted on 18 adult horses (3–15 years old). Based on the treatment regimen utilized, the horses were divided into three groups (n = 6), with each group having a similar mean wound area. The percentages of wound contraction, epithelialization, and overall wound healing were determined weekly for each wound. By the end of the study, the total wound healing percentage was significantly increased between the study groups (p < 0.05). The use of maltodextrin/ascorbic acid gel resulted in considerable wound contraction, rapid epithelialization, and complication-free wound healing. Based on the findings of this study, maltodextrin/ascorbic acid gel, independently or in combination with a 1% povidone-iodine solution, might be applied as a safe and effective wound healing promoting agent in horses with chronic non-granulating wounds.
  • Anesthetic induction with propofol versus ketamine pre and post lower pole nephrectomy in dogs
    Life Science Journal, 2013
  • Effect of magnesium sulphate on duration and dose requirement of thiopental sodium anesthesia in dogs undergoing experimental enterotomy
    IBRAHIM HELAL, MOHAMED EL DAHRAWY
    Indian Journal of Animal Sciences, 2012
  • Human amniotic membrane and vitamin E /selenium for control of postoperative adhesion in dogs
    Japanese Journal of Veterinary Research, 2011
  • Effect of exercise on serum concentration of cartilage oligomeric matrix protein in thoroughbreds
    Ibrahim E. Helal, Kazuhiro Misumi, Oki Tateno, Takeshi Kodama, Mutsuki Ishimaru, Junya Yamamoto, Daiske Miyakoshi, Makoto Fujiki
    American Journal of Veterinary Research, 2007
  • Urine cartilage oligomeric matrix protein (COMP) measurement is useful in discriminating the osteoarthritic Thoroughbreds
    K. Misumi, M. Tagami, T. Kamimura, D. Miyakoshi, I.E. Helal, K. Arai, M. Fujiki
    Osteoarthritis and Cartilage, 2006