Phenotypic and genotypic characterization of electron beam treated inter-specific (Triticum dicoccum Schrank X Triticum carthlicum Nevski) lines of emmer wheat for leaf rust and stem rust resistance Geetha Murugan, Bikram Kishore Das, Sivasamy Murugasamy, Jayaprakash Paramasivan, Gautam Vishwakarma, Abhijit Shaligram Shitre, Pramod Prasad, Subodh Kumar, Appunu Chinnaswamy, Yashavathakumar Kakanur Jagadeesha, Sudhir Navathe, Suma Biradar, John Peter, Suganya Chinnaswamy, Vaishali Gopu, Harish Chandar Subbiah Gounder Rajendran, Lalitha Gundeappa, Marimuthu Kanchana, Vikas Venu Kumaran International Journal of Radiation Biology, 2025 Purpose Cultivated emmer wheat (Triticum dicoccum), a hulled tetraploid species (2n = 4x = 28, AABB) is a minor crop grown in India for its low glycaemic index and high protein (16%) content. However, its genetic variability is limited. This study aims to enhance the variability of cultivated emmer wheat using mutation approach.Materials and methods A tall better yielding, leaf and stem rust resistant inter-specific line (BC2F2 line derived from Triticum dicoccum cv. NP200 X Triticum carthlicum) was subjected to physical mutagens (gamma rays and electron beam), with the objective to develop a semi-dwarf, high-yielding, leaf rust and stem rust resistant lines. Mutant and parental lines were evaluated for resistance to leaf rust and stem rust at seedling and adult plant stages and also validated the presence of rust resistance genes through linked molecular markers.Results Three stable semi-dwarf inter-specific mutant lines with high yield and resistance to leaf rust and stem rust were selected from the electron beam (250 Gy) treated mutagenized population. Seedling and adult plant evaluations displayed resistance to leaf rust and stem rust and molecular marker analyses confirmed the presence of linked marker to stem rust resistance genes (Sr2/Lr27/Yr30, Sr17/Lr14a/Pm5 and Sr40) and leaf rust resistance genes (Lr18, Lr50 and Lr61) in mutant and parental lines. In addition, plant stature, culm strength and yield improvement was observed as compared to parental lines.Conclusion The resistance present in the parent lines remained intact in the selected mutant lines even after exposure to electron beam. Mutants thus obtained could be released as cultivars or utilized as a potential source for high yielding, rust resistance for the improvement of emmer wheat. This is the first report of beneficial mutants in emmer wheat using electron beam.
Role of lens and dormancy breaking treatment in Fabaceae seeds (Acacia auriculiformis A.Cunn. ex Benth., Delonix regia (Bojer ex Hook.) Raf. Cassia fistula L., Samanea saman (Jacq.) Merr., Pongamia pinnata L.) Preethi Jenifer S. Praticia, C. Krishnaveni, M. Kanchana Research Journal of Biotechnology, 2023 Seeds of leguminous plants are generally considered as orthodox seed. However, the conditions for combinations of dormancy, as well as the location of water gaps in Fabaceae seeds remain unclear. In the present study, we consider the adaptive role of impermeable coats in the seeds of Acacia auriculiformis A.Cunn. ex Benth., Delonix regia (Bojer ex Hook.) Raf. Cassia fistula L., Samanea saman (Jacq.) Merr., Pongamia pinnata L. The study was specially designed to analyze the condition that broke physical dormancy and locating primary water gap during dormancy breaking. Seeds were treated in hot water to break its physical dormancy and it was observed that water entered only through the lens due to dislodgement of palisade layer. In addition to extension of storage life of Delonix regia seed, it was proved by analyzing the low moisture content of 5.31%. This study concludes that the impermeable seed coat of seeds with low moisture content will increases storage life and seed imbibition adjusts germination with the growing season.
Isolation and differential expression of sodium hydrogen antiporter (NHX1) genes in leucas aspera Plant Cell Biotechnology and Molecular Biology, 2019
Leucas aspera – A Review of its Biological activity Kulandaisamy Agnes Nirmala, Marimuthu Kanchana Systematic Reviews in Pharmacy, 2018 Herbal plants are integral parts of the traditional medicine worldwide and most of the rural and urban population uses these plants in many of their regular needs even today. The current researchers are more focused on natural chemicals than the synthetic chemicals due to their environmental, economical and health benefits. Plants produce many chemical compounds for its biological activities including defence mechanism against microbes, insects and herbivorous animals and these chemicals are called as phyto chemicals. Herbal plants are a natural source of many important phyto chemicals and widely used in Pharmaceutical, food and cosmetic industries. A wide variety of herbal plants are available in the Indian subcontinent and they are the backbone of Indian traditional medicines Ayurveda and Siddha. Leucas aspera Linn., commonly known as Thumbai or Dronpushpi is a small, erect, branched annual aromatic herb, distributed throughout India from the Himalayas down to Ceylon. Though it grows as a weed throughout the country, it has a number of medicinal values and it is used in traditional medicine to treat many diseases. The study of various biological properties of this plant will be useful for its potential use in respective industries. Medicinally, it has been proven to possess various pharmacological values like antifungal, antipyretic, antioxidant, antimicrobial, antinociceptive, analgesic, antidiarrheal, insecticidal, antiinflammatory and cytotoxic activity in a number of studies by different researchers in the past several years. The available reports on antimicrobial activity, antioxidant activity and pharmacological value of L. aspera are discussed in this review.
Engineering sugarcane cultivars with bovine pancreatic trypsin inhibitor (aprotinin) gene for protection against top borer (Scirpophaga excerptalis Walker) Leela Amala Christy, S. Arvinth, M. Saravanakumar, M. Kanchana, N. Mukunthan, J. Srikanth, George Thomas, N. Subramonian Plant Cell Reports, 2009 The inhibitory activity of bovine pancreatic trypsin inhibitor (aprotinin), a natural polypeptide and a proteinase inhibitor, was demonstrated on gut proteinases of three lepidopteran borers of sugarcane using commercially available aprotinin. A synthetic gene coding for aprotinin, designed and codon optimized for better expression in plant system (Shantaram 1999), was transferred to two sugarcane cultivars namely CoC 92061 and Co 86032 through particle bombardment. Aprotinin gene expression was driven by maize ubiquitin promoter and the plant selection marker used was hygromycin resistance. The integration, expression and functionality of the transgene was confirmed by Southern, Western and insect bioassay, respectively. Southern analysis showed two to four integration sites of the transgene in the transformed plants. Independent transgenic events showed varied levels of transgene expression resulting in different levels (0.16–0.50%) of aprotinin. In in vivo bioassay studies, larvae of top borer Scirpophaga excerptalis Walker (Lepidoptera: Pyralidae) fed on transgenics showed significant reduction in larval weight which indicated impairment of their development. Results of this study show the possibility of deploying aprotinin gene for the development of transgenic sugarcane cultivars resistant to top borer.
Auxin pretreatment promotes regeneration of sugarcane (Saccharum spp. hybrids) midrib segment explants G. Franklin, S. Arvinth, C. J. Sheeba, M. Kanchana, N. Subramonian Plant Growth Regulation, 2006 We have developed a new, simple, quick and genotype-independent method for direct regeneration of sugarcane using novel midrib segment explants. Our protocol involves two steps: the pretreatment of starting material on MS (Murashige and Skoog (1962) Physiol Plant 15:473–497) medium containing 3.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 days under continuous dark and subsequent transfer of the explants to MS medium augmented with 0.1 mg/l benzyladenine (BA) and 0.1 mg/l naphthaleneacetic acid (NAA) under light-dark conditions. On the regeneration medium, numerous globular structures appeared from the explants and subsequently differentiated into shoots. Regenerated shoots attained 2–5 cm height within 30 days of culture initiation and readily rooted on MS basal medium. Hardened plants were successfully established in the greenhouse. The regulation of sugarcane morphogenesis by auxin pretreatment is discussed.
RECENT SCHOLAR PUBLICATIONS
ISOLATION AND DIFFERENTIAL EXPRESSION OF SODIUM HYDROGEN ANTIPORTER (NHX1) GENES IN Leucas aspera KA Nirmala, M Kanchana Plant Cell Biotechnology and Molecular Biology 20 (13-14), 541-547 , 2019 2019.0
Isolation and Molecular characterization of phosphate solubilizing fungi from Rhizospheric soil SJMK V. Sujithra, M. Sankavi, S. Nathiya, H. Sreenithi, B. Aarthika, C ... INTERNATIONAL JOURNAL OF PHARMACY AND BIOLOGICAL SCIENCES 9 (2), 507-514 , 2019 2019.0
Leucas aspera-A Review of its Biological activity. KA Nirmala, M Kanchana Systematic Reviews in Pharmacy 9 (1) , 2018 2018.0 Citations: 49
Promoter diversity in plants–a review M Saranya, M Kanchana International Journal of Applied and Advanced Scientific Research 1 (1), 209-217 , 2016 2016.0 Citations: 5
Composting of Areca nut leaf sheath and its effects on growth and biochemical contents of Vigna unguiculata L. PU Maheswari, M Saranya, K Agnes, M Kanchana International Research Journal of Public and Environmental Health 2 (2), 23-26 , 2015 2015.0 Citations: 9
Preliminary Phyto-chemical analysis of various parts of indigenous medicinal plant Cissus quadranglaris. (Vitaceae) M Navaroja, R. D., and Kanchana International Journal and Pharmaceutical research Scholars. 2 (4), 443-447 , 2013 2013.0
Observations of Medicinal Plants Used By Kukna Tribes of Jhavada Villages of Waghai Forest, Dangs DT, Gujarat, India. DR Navaroja, M Kanchana 2012.0 Citations: 3
Comparative lead uptake and responses of five different plant species grown on lead contaminated soil DR Navaroja, M Kanchana Bioscan, 7, 225-227 , 2012 2012.0 Citations: 1
Optimization of extracellular alkaline protease enzyme from Bacillus sp M Kanchana, S Padmavathy Bioscan 5 (1), 85-87 , 2010 2010.0 Citations: 9
Engineering sugarcane cultivars with bovine pancreatic trypsin inhibitor (aprotinin) gene for protection against top borer ( Scirpophaga excerptalis Walker) LA Christy, S Arvinth, M Saravanakumar, M Kanchana, N Mukunthan, ... Plant cell reports 28 (2), 175-184 , 2009 2009.0 Citations: 101
Auxin pretreatment promotes regeneration of sugarcane ( Saccharum spp. hybrids) midrib segment explants G Franklin, S Arvinth, CJ Sheeba, M Kanchana, N Subramonian Plant Growth Regulation 50 (2), 111-119 , 2006 2006.0 Citations: 71
Green Synthesis and Phytochemical Analysis of Silver Nano Particles Using Root of Nelumbo nucifera and their Antibacterial Activity M Kanchana, J Dharshni, M S Geetha, V Sujithra
MOST CITED SCHOLAR PUBLICATIONS
Engineering sugarcane cultivars with bovine pancreatic trypsin inhibitor (aprotinin) gene for protection against top borer ( Scirpophaga excerptalis Walker) LA Christy, S Arvinth, M Saravanakumar, M Kanchana, N Mukunthan, ... Plant cell reports 28 (2), 175-184 , 2009 2009.0 Citations: 101
Auxin pretreatment promotes regeneration of sugarcane ( Saccharum spp. hybrids) midrib segment explants G Franklin, S Arvinth, CJ Sheeba, M Kanchana, N Subramonian Plant Growth Regulation 50 (2), 111-119 , 2006 2006.0 Citations: 71
Leucas aspera-A Review of its Biological activity. KA Nirmala, M Kanchana Systematic Reviews in Pharmacy 9 (1) , 2018 2018.0 Citations: 49
Composting of Areca nut leaf sheath and its effects on growth and biochemical contents of Vigna unguiculata L. PU Maheswari, M Saranya, K Agnes, M Kanchana International Research Journal of Public and Environmental Health 2 (2), 23-26 , 2015 2015.0 Citations: 9
Optimization of extracellular alkaline protease enzyme from Bacillus sp M Kanchana, S Padmavathy Bioscan 5 (1), 85-87 , 2010 2010.0 Citations: 9
Promoter diversity in plants–a review M Saranya, M Kanchana International Journal of Applied and Advanced Scientific Research 1 (1), 209-217 , 2016 2016.0 Citations: 5
Observations of Medicinal Plants Used By Kukna Tribes of Jhavada Villages of Waghai Forest, Dangs DT, Gujarat, India. DR Navaroja, M Kanchana 2012.0 Citations: 3
Comparative lead uptake and responses of five different plant species grown on lead contaminated soil DR Navaroja, M Kanchana Bioscan, 7, 225-227 , 2012 2012.0 Citations: 1
ISOLATION AND DIFFERENTIAL EXPRESSION OF SODIUM HYDROGEN ANTIPORTER (NHX1) GENES IN Leucas aspera KA Nirmala, M Kanchana Plant Cell Biotechnology and Molecular Biology 20 (13-14), 541-547 , 2019 2019.0
Isolation and Molecular characterization of phosphate solubilizing fungi from Rhizospheric soil SJMK V. Sujithra, M. Sankavi, S. Nathiya, H. Sreenithi, B. Aarthika, C ... INTERNATIONAL JOURNAL OF PHARMACY AND BIOLOGICAL SCIENCES 9 (2), 507-514 , 2019 2019.0
Preliminary Phyto-chemical analysis of various parts of indigenous medicinal plant Cissus quadranglaris. (Vitaceae) M Navaroja, R. D., and Kanchana International Journal and Pharmaceutical research Scholars. 2 (4), 443-447 , 2013 2013.0
Green Synthesis and Phytochemical Analysis of Silver Nano Particles Using Root of Nelumbo nucifera and their Antibacterial Activity M Kanchana, J Dharshni, M S Geetha, V Sujithra
INDUSTRY EXPERIENCE
papain industry
Alkaline protease enzyme
Tofu production
