Attenuation of reactive nitrogen species by different flavonoids enriched fractions of Schima Wallichii Sanchita Das, Asis Bala, Manas Bhowmik, Lakshmi Kanta Ghosh Asian Pacific Journal of Tropical Biomedicine, 2012 To investigate Schima wallichii (S. wallichii) Choisy (Ternstroemiaceae) which is a well known plant of Sikkim in the Himalayan region, India. Therefore three major flavonoid enriched fractions (FPet.Ether, FChloroform and FEthylacetate) were isolated by petroleum ether chloroform and ethyl acetate successively. The reactive nitrogen species scavenging activity of the flavonoid fractions was established using biochemical assay to measure scavenging of 2, 2 diphenyl picrylhydrazyl (DPPH), nitric oxide (NO) and peroxinitrite. FEthylacetate showed maximum scavenging activity: their IC50 being (7.33 ± 3.32), (7.11 ± 2.21), and (6.67 ± 2.23) μg/mL in DPPH, NO, peroxinitrite radical respectively. Presence of (57.32 ± 2.31) and (163.4 ± 2.22) μg of flavonoids and phenolic compound in 1 mg of extract is assumed to be responsible for free radical scavenging activity. Taken together S. wallichii has potent free radical scavenging property indicating its importance in food supplement as a rich source of active flavonoid and phenolic compounds in ethyl acetate fraction which is responsible for its free radical scavenging as well as antioxidant activity.
Study of thermo-sensitive in-situ gels for ocular delivery Manas BHOWMIK Scientia Pharmaceutica, 2011 The aim of the present study was the development of thermo-sensitive in-situ gels for in-vitro evaluation of ophthalmic delivery systems of ketorolac tromethamine (KT), based on methylcellulose (MC) in combination with hydroxypropylmethyl cellulose (HPMC). The gel temperature of 1% MC solution was observed at 60°C. It was found that 6% oral rehydration salt without dextrose (ORS) was capable to reduce the gel temperature below physiological temperature. HPMC was added to increase viscosity and drug release time. The results indicated a large increase in viscosity at 37°C with addition of HPMC whch provided sustained release of the drug over a 4h period. From in-vitro release studies, it could be concluded that the developed systems were thus a better alternative to conventional eye drops.
Methyl cellulose based sustained release thermosensitive in situ fast gelling ocular delivery of ketorolac tromethamine Asian Journal of Chemistry, 2010
Hepatoprotective and antioxidant activities of Smilax Chinensis L. root Pharmacologyonline, 2008
