Lipophilic bioactive compounds from thermophilic cyanobacterium Leptolyngbya sp. HNBGU-004: Implications for countering VRSA resistance Sachin Tyagi, Rahul Kunwar Singh, Ashok Kumar Heliyon, 2024 Extremophiles thrive in extreme conditions, showcasing rich and unexplored diversity. This resilience hints at the existence of novel biochemical pathways and unique bioactive compounds. In contrast, the issue of drug resistance and excessive misuse of antibiotics in various settings, such as healthcare, agriculture, and veterinary medicine, has contributed to the emergence and spread of drug-resistant microorganisms. In the present research, Leptolyngbya sp. HNBGU-004, was obtained from an extreme location, a hot water spring in the Garhwal Himalayan region of India. The lipophilic fraction derived from Leptolyngbya sp. HNBGU-004 exhibited significant inhibitory effects against vancomycin-resistant Staphylococcus aureus (VRSA), displaying a bactericidal concentration of 0.5 mg mL -1 . Furthermore, gas chromatography-mass spectrometry (GC-MS) analysis of the lipophilic extract unveiled the major constituents. Leptolyngbya sp. HNBGU-004 holds significant promise as a primary source of potent anti-vancomycin-resistant S. aureus components. These findings emphasize the importance of Leptolyngbya sp. HNBGU-004 as a foundational source for use as both a synergistic and alternative agent against VRSA.
Analysis of noise pollution level in and around SIDCUL area in District Haridwar (Uttarakhand) India Pankaj Saini, Navneet Sharma, Ashok Kumar, Sachin Tyagi, Sangeeta Sharma Environment Conservation Journal, 2023 The primary objective of this study is to evaluate the issue of noise pollution in the vicinity of the industrial zone of Haridwar city and its correlation with the health of the people in and around the SIDCUL (State Industrial Development Corporation of Uttarakhand Limited). The study revealed that noise pollution levels were above the CPCB Standard for the sound level for industrial zones and residential zones. During the period from January to December 2018, noise levels were observed and monitored in both the industrial zone (Site-I SIDCUL) and the residential zone (Site-II Siwalik Nagar). The average noise levels varied throughout the year. At Site-I (Industrial Zone), during the daytime in July, the maximum average noise level recorded was 89.5 dB, while in April; the minimum average noise level was 81.1 dB. During nighttime, in January, the maximum average noise level reached 84.1 dB, and in May, the minimum average noise level was 76.6 dB. In Site-II Siwalik Nagar (Residential Zone), the daytime noise levels were a maximum average noise level of 61.1 dB in May and a minimum average noise level of 58.8 dB in September. During nighttime, the noise level reached a maximum average of 47.2 dB in October and a minimum average of 44.5 dB in May. Overall, the study revealed that noise levels were generally higher in both zones, except for the average nighttime noise level at Site II (residential zone), which was below the prescribed standard limit for noise.
Bioremediation potential of green wastes and plant growth promoting rhizobacteria and its enhancement by their combination: A review Ashok Kumar, Sai Prakash Naroju, Mrinalini Langthasa, Itishree Behera, Sachin Tyagi, Navneet Sharma, Rishi Kumar Gautam Environmental Advances, 2023 The increasing anthropogenic and technogenic activities to compensate the raising population and unending demands of humans ended in severe pollution and detrimental damage to the environment. This environmental pollution due to lethal pollutants, toxic heavy metals and organic wastes has been drastically affecting the ecosystem of the living organisms. These are forced to enter into the food chain as they tend to accumulate in the agricultural soils. In order to eliminate these pollutants from the soils the bioremediation will be an efficient tool and this can be achieved by plant growth promoting rhizobacteria and by green wastes. In this study the plant growth promoting rhizobacteria (PGPR) and green wastes are evaluated for their effectiveness in bioremediating the toxic contaminants. Green wastes are rich sources of naturally occurring polyphenols which are potential eliminating agents of these pollutants, they can perform metal chelation, reduction, antibiotic properties, adsorption, complexation and by supplying the nutrients. However, PGPRs are well known plant life saviors from various biotic and abiotic stresses; they are also the bioremediating agents as they perform heavy metal elimination by various methods. In this study, it is also depicted that the combined application of PGPR and green wastes result in the significant method to bioremediate the contaminated sites. The bioremediation by PGPRs and green wastes is the prominent effective way to terminate the pollutants compared to phytoremediation.
Anti-enterococcal and anti-oxidative potential of a thermophilic cyanobacterium, Leptolyngbya sp. HNBGU 003 Sachin Tyagi, Rahul Kunwar Singh, Shree Prakash Tiwari Saudi Journal of Biological Sciences, 2021 Enterococci, the opportunistic pathogens, pose several serious and life-threatening infections such as urinary tract infections, sepsis, and endocarditis. The situation is worsening due to the development of drug resistance in these pathogens against several antibiotics. The addition of anti-enterococcal compounds with antioxidant activity in fermented and packaged food may help prevent the transmission of food-borne enterococcal infections. Scientists are in continuous search of such compounds from various sources. Hence, the present study has tested the diethyl ether extracts of thermophilic cyanobacteria, selected based on a previous study, against the multidrug-resistant and -sensitive strains of Enterococcus faecium. Out of the eleven tested extracts, 72% have shown anti-enterococcal activity against both strains. Among the extracts with anti-enterococcal activity, the diethyl ether extract of Leptolyngbya sp. (DEEL-3) inhibited the growth of VRE in a dose-dependent manner with a minimum inhibitory concentration of 2.0 mg mL-1. The DEEL-3 has also shown its antioxidant potential in terms of DPPH scavenging with an IC50 of 3.16 mg mL-1. The organism was named Leptolyngbya sp. HNBGU 003 based on 16SrRNA sequence homology analysis and morphological features. Further, the GC–MS analysis of the DEEL-3 has revealed the predominance of two phenolic compounds, phenol, 2,4-bis(1,1-dimethylethyl)-, phosphite (3:1) and tris(2,4-di-tert-butylphenyl) phosphate, in it. Thus, the anti-enterococcal and antioxidant activity of DEEL-3 may be attributed to these phenolics, which may be isolated and developed as food additives.
Cyanobacteria: A new terminus for anti-infectious agents Role of Photosynthetic Microbes in Agriculture and Industry, 2018
