Chemistry, Organic Chemistry, Process Chemistry and Technology, Radiology, Nuclear Medicine and imaging
18
Scopus Publications
900
Scholar Citations
15
Scholar h-index
17
Scholar i10-index
Scopus Publications
Hexafluoroisopropanol as a Bioconjugation Medium of Ultrafast, Tryptophan-Selective Catalysis Mohammad Nuruzzaman, Brandon M. Colella, Chiamaka P. Uzoewulu, Alissa E. Meo, Elizabeth J. Gross, et al. Journal of the American Chemical Society, 2024 The past decade has seen a remarkable growth in the number of bioconjugation techniques in chemistry, biology, material science, and biomedical fields. A core design element in bioconjugation technology is a chemical reaction that can form a covalent bond between the protein of interest and the labeling reagent. Achieving chemoselective protein bioconjugation in aqueous media is challenging, especially for generally less reactive amino acid residues, such as tryptophan. We present here the development of tryptophan-selective bioconjugation methods through ultrafast Lewis acid-catalyzed reactions in hexafluoroisopropanol (HFIP). Structure-reactivity relationship studies have revealed a combination of thiophene and ethanol moieties to give a suitable labeling reagent for this bioconjugation process, which enables modification of peptides and proteins in an extremely rapid reaction unencumbered by noticeable side reactions. The capability of the labeling method also facilitated radiofluorination application as well as antibody functionalization. Enhancement of an α-helix by HFIP leads to its compatibility with a certain protein, and this report also demonstrates a further stabilization strategy achieved by the addition of an ionic liquid to the HFIP medium. The nonaqueous bioconjugation approaches allow access to numerous chemical reactions that are unavailable in traditional aqueous processes and will further advance the chemistry of proteins.
Regioselective ortho-sulfonamidation: Exploration of Intrinsic Directing Property of β-Carbolines and their Photophysical Studies Darshana Bora, Srinivas Reddy Dannarm, Stephy Elza John, Sravani Sana, Rajesh Sonti, et al. Asian Journal of Organic Chemistry, 2021 A regioselective ruthenium‐catalysed strategy for synthesising mono‐, ortho‐sulfonamidated β‐carboline derivatives using sulfonyl azide was accomplished by relying on the intrinsic directing property of β‐carbolines. This protocol is apt for all types of substitutions with compelling yields. We have investigated the reaction mechanism using ESI‐MS by arresting the intermediates associated with this selective transformation. The achieved molecules were also surfaced with potential percentage inhibition against cancer cell lines. In addition, practical and computational analysis on these new scaffolds demonstrated photophysical properties.
Synthesis and preclinical evaluation of indole triazole conjugates as microtubule targeting agents that are effective against MCF-7 breast cancer cell lines Vidyasrilekha Yele, Sai Kiran S.S. Pindiprolu, Sravani Sana, D.S.V.N.M. Ramamurty, Jayanthi R.K. Madasi, et al. Anti Cancer Agents in Medicinal Chemistry, 2021 Background: Microtubules are considered to be an important therapeutic target for most of the anticancer drugs. These are highly dynamic structures comprising of α-tubulin and β-tubulin which are usually heterodimers and found to be involved in cell movement, intracellular trafficking, and mitosis inhibition of which might kill the tumour cells or inhibit the abnormal proliferation of cells. Most of the tubulin polymerization inhibitors, such as Vinca alkaloids, consist of Indole as the main scaffold. The literature also suggests using triazole moiety in the chemical entities, potentiating the inhibitory activity against cell proliferation. So, in our study, we used indole triazole scaffolds to synthesize the derivatives against tubulin polymerization. Objective: The main objective of this study to synthesize indole triazole conjugates by using environmentally friendly solvents (green chemistry) and click chemistry. To carry out the MTT assay and tubulin polymerization assay for the synthesized indole triazole conjugates. Methods: All the synthesized molecules were subjected to molecular docking studies using Schrodinger suite and the structural confirmation was performed by Mass, proton-NMR and carbon-NMR, documented in DMSO and CDCL3. Biological studies were performed using DU145 (prostate cancer), A-549 (lung cancer) and, MCF-7 (breast cancer), cell lines obtained from ATCC were maintained as a continuous culture. MTT assay was performed for the analogues using standard protocol. Cell cycle analysis was carried out using flow cytometry. Results: The Indole triazole scaffolds were synthesized using the principles of Green chemistry. The triazole formation is mainly achieved by using the Click chemistry approach. Structural elucidation of synthesized compounds was performed using Mass spectroscopy (HR-MS), Proton-Nuclear Magnetic Spectroscopy (1H-NMR) and Carbon-Nuclear Magnetic Spectroscopy (13C-NMR). The XP-docked poses and free energy binding calculations revealed that 2c and 2g molecules exhibited the highest docking affinity against the tubulin-colchicine domain (PDB:1SA0). In vitro cytotoxic assessment revealed that 2c and 2g displayed promising cytotoxicity in MTT assay (with CTC50 values 3.52μM and 2.37μM) which are in good agreement with the computational results. 2c and 2g also arrested 63 and 66% of cells in the G2/M phase, respectively, in comparison to control cells (10%) and tubulin polymerization inhibition assay revealed that 2c and 2g exhibited significant inhibition of tubulin polymerization with IC50 values of 2.31μM, and 2.62μM, respectively in comparison to Nocodazole, a positive control, resulted in an IC50 value of 2.51μM. Conclusion: Indole triazole hybrids were synthesized using click chemistry, and docking studies were carried out using Schrodinger for the designed molecules. Process Optimization has been done for both the schemes. Twelve compounds (2a-2l) have been successfully synthesized and analytical evaluation was performed using NMR and HR-MS. In vitro evaluation was for the synthesized molecules to check tubulin polymerization inhibition for antiproliferative action. Among the synthesized compounds, 2c and 2g have potent anticancer activities by inhibiting tubulin polymerization.
Synthesis and Evaluation of Bispecific 64Cu—Radio conjugates for PET Imaging in Prostate Cancer Models S Sana, X Ma, W Zhao, S Cheng, X Wu, X Ni, Z Wu, Z Li Journal of Nuclear Medicine 66 (supplement 1), 252187-252187 , 2025 2025
Synthesis and Evaluation of FAPI-Targeted 64Cu— Radioconjugates Containing Variable PEG Linkers for PET Imaging in U87 Cancer Models S Sana, X Ma, K Rakesh, W Zhao, Z Wu, Z Li Journal of Nuclear Medicine 65 (supplement 2), 242489-242489 , 2024 2024
Synthesis and preclinical characterization of the 64Cu labeled TRPC5-targeting PET imaging probe for cancer X Ma, S Sana, Z Xing, Y Yu, W Zhao, Z Wu, Z Tu, Z Li Journal of Nuclear Medicine 65 (supplement 2), 242385-242385 , 2024 2024
Hexafluoroisopropanol as a bioconjugation medium of ultrafast, tryptophan-selective catalysis M Nuruzzaman, BM Colella, CP Uzoewulu, AE Meo, EJ Gross, S Ishizawa, ... Journal of the American Chemical Society 146 (10), 6773-6783 , 2024 2024 Citations: 28
Visible-light-mediated photocatalytic sequential N-arylation: an eco-friendly synthetic route to unsymmetrical diarylamines and the imatinib drug S Sana, S Dastari, DS Reddy, R Tokala, M Sathish, R Sonti, ... Organic Chemistry Frontiers 10 (18), 4573-4580 , 2023 2023 Citations: 7
Sustainable photocatalytic C–H annulation of heteroarenes with sulfoxonium ylides: synthesis and photophysical properties of fused imidazo [1, 2-a] pyridine-based molecules S Sana, SR Dannarm, R Tokala, S Dastari, M Sathish, R Kumar, R Sonti, ... Organic Chemistry Frontiers 10 (19), 4800-4808 , 2023 2023 Citations: 15
Regioselective ortho ‐sulfonamidation: Exploration of Intrinsic Directing Property of β‐Carbolines and their Photophysical Studies D Bora, S Reddy Dannarm, S Elza John, S Sana, R Sonti, N Shankaraiah Asian Journal of Organic Chemistry 10 (12), 3384-3389 , 2021 2021 Citations: 17
Synthesis and biological evaluation of novel imidazo [1, 2-a] pyridine-oxadiazole hybrids as anti-proliferative agents: Study of microtubule polymerization inhibition and DNA … DK Sigalapalli, G Kiranmai, GP Devi, R Tokala, S Sana, C Tripura, ... Bioorganic & Medicinal Chemistry 43, 116277 , 2021 2021 Citations: 26
Cinnamide derived pyrimidine-benzimidazole hybrids as tubulin inhibitors: synthesis, in silico and cell growth inhibition studies S Sana, VG Reddy, TS Reddy, R Tokala, R Kumar, SK Bhargava, ... Bioorganic Chemistry 110, 104765 , 2021 2021 Citations: 76
Synthesis and preclinical evaluation of indole triazole conjugates as microtubule targeting agents that are effective against MCF-7 breast cancer cell lines V Yele, SKSS Pindiprolu, S Sana, D Ramamurty, JRK Madasi, ... Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal … , 2021 2021 Citations: 21
Palladium catalyzed C–C and C–N bond forming reactions: an update on the synthesis of pharmaceuticals from 2015–2020 J Rayadurgam, S Sana, M Sasikumar, Q Gu Organic Chemistry Frontiers 8 (2), 384-414 , 2021 2021 Citations: 222
Development of β-carboline-benzothiazole hybrids via carboxamide formation as cytotoxic agents: DNA intercalative topoisomerase IIα inhibition and apoptosis induction R Tokala, S Mahajan, G Kiranmai, DK Sigalapalli, S Sana, SE John, ... Bioorganic Chemistry 106, 104481 , 2021 2021 Citations: 54
Design and synthesis of thiadiazolo-carboxamide bridged β-carboline-indole hybrids: DNA intercalative topo-IIα inhibition with promising antiproliferative activity R Tokala, S Sana, UJ Lakshmi, P Sankarana, DK Sigalapalli, N Gadewal, ... Bioorganic Chemistry 105, 104357 , 2020 2020 Citations: 43
Exploration of carbamide derived pyrimidine-thioindole conjugates as potential VEGFR-2 inhibitors with anti-angiogenesis effect S Sana, VG Reddy, S Bhandari, TS Reddy, R Tokala, AP Sakla, ... European Journal of Medicinal Chemistry 200, 112457 , 2020 2020 Citations: 88
Ring-opening cyclization of activated spiro-aziridine oxindoles with heteroarenes: a facile synthetic approach to spiro-oxindole-fused pyrroloindolines S Bhandari, S Sana, V Lahoti, R Tokala, N Shankaraiah RSC advances 10 (27), 16101-16109 , 2020 2020 Citations: 14
Design and Synthesis of Substituted Dihydropyrimidinone Derivatives as Cytotoxic and Tubulin Polymerization Inhibitors S Sana, R Tokala, DM Bajaj, N Nagesh, KK Bokara, G Kiranmai, ... Bioorganic Chemistry, 103317 , 2019 2019 Citations: 57
Ru (II)-catalyzed regioselective hydroxymethylation of β-carbolines and isoquinolines via C–H functionalization: probing the mechanism by online ESI-MS/MS screening R Tokala, D Bora, S Sana, FM Nachtigall, LS Santos, N Shankaraiah The Journal of Organic Chemistry 84 (9), 5504-5513 , 2019 2019 Citations: 39
Microwave‐Assisted One‐Pot [3+ 2] Cycloaddition of Azomethine Ylides and 3‐Alkenyl Oxindoles: A Facile Approach to Pyrrolidine‐Fused Bis‐Spirooxindoles S Bhandari, S Sana, B Sridhar, N Shankaraiah ChemistrySelect 4 (5), 1727-1730 , 2019 2019 Citations: 34
Design and synthesis of DNA‐interactive β‐carboline–oxindole hybrids as cytotoxic and apoptosis‐inducing agents R Tokala, S Thatikonda, US Vanteddu, S Sana, C Godugu, ... ChemMedChem 13 (18), 1909-1922 , 2018 2018 Citations: 64
Synthesis and in vitro cytotoxicity evaluation of β-carboline-linked 2, 4-thiazolidinedione hybrids: potential DNA intercalation and apoptosis-inducing studies R Tokala, S Thatikonda, S Sana, P Regur, C Godugu, N Shankaraiah New Journal of Chemistry 42 (19), 16226-16236 , 2018 2018 Citations: 63
MOST CITED SCHOLAR PUBLICATIONS
Palladium catalyzed C–C and C–N bond forming reactions: an update on the synthesis of pharmaceuticals from 2015–2020 J Rayadurgam, S Sana, M Sasikumar, Q Gu Organic Chemistry Frontiers 8 (2), 384-414 , 2021 2021 Citations: 222
Exploration of carbamide derived pyrimidine-thioindole conjugates as potential VEGFR-2 inhibitors with anti-angiogenesis effect S Sana, VG Reddy, S Bhandari, TS Reddy, R Tokala, AP Sakla, ... European Journal of Medicinal Chemistry 200, 112457 , 2020 2020 Citations: 88
Cinnamide derived pyrimidine-benzimidazole hybrids as tubulin inhibitors: synthesis, in silico and cell growth inhibition studies S Sana, VG Reddy, TS Reddy, R Tokala, R Kumar, SK Bhargava, ... Bioorganic Chemistry 110, 104765 , 2021 2021 Citations: 76
Design and synthesis of DNA‐interactive β‐carboline–oxindole hybrids as cytotoxic and apoptosis‐inducing agents R Tokala, S Thatikonda, US Vanteddu, S Sana, C Godugu, ... ChemMedChem 13 (18), 1909-1922 , 2018 2018 Citations: 64
Synthesis and in vitro cytotoxicity evaluation of β-carboline-linked 2, 4-thiazolidinedione hybrids: potential DNA intercalation and apoptosis-inducing studies R Tokala, S Thatikonda, S Sana, P Regur, C Godugu, N Shankaraiah New Journal of Chemistry 42 (19), 16226-16236 , 2018 2018 Citations: 63
Design and Synthesis of Substituted Dihydropyrimidinone Derivatives as Cytotoxic and Tubulin Polymerization Inhibitors S Sana, R Tokala, DM Bajaj, N Nagesh, KK Bokara, G Kiranmai, ... Bioorganic Chemistry, 103317 , 2019 2019 Citations: 57
Development of β-carboline-benzothiazole hybrids via carboxamide formation as cytotoxic agents: DNA intercalative topoisomerase IIα inhibition and apoptosis induction R Tokala, S Mahajan, G Kiranmai, DK Sigalapalli, S Sana, SE John, ... Bioorganic Chemistry 106, 104481 , 2021 2021 Citations: 54
Design and synthesis of thiadiazolo-carboxamide bridged β-carboline-indole hybrids: DNA intercalative topo-IIα inhibition with promising antiproliferative activity R Tokala, S Sana, UJ Lakshmi, P Sankarana, DK Sigalapalli, N Gadewal, ... Bioorganic Chemistry 105, 104357 , 2020 2020 Citations: 43
Ru (II)-catalyzed regioselective hydroxymethylation of β-carbolines and isoquinolines via C–H functionalization: probing the mechanism by online ESI-MS/MS screening R Tokala, D Bora, S Sana, FM Nachtigall, LS Santos, N Shankaraiah The Journal of Organic Chemistry 84 (9), 5504-5513 , 2019 2019 Citations: 39
Microwave‐Assisted One‐Pot [3+ 2] Cycloaddition of Azomethine Ylides and 3‐Alkenyl Oxindoles: A Facile Approach to Pyrrolidine‐Fused Bis‐Spirooxindoles S Bhandari, S Sana, B Sridhar, N Shankaraiah ChemistrySelect 4 (5), 1727-1730 , 2019 2019 Citations: 34
Vanadium-Catalyzed Oxidative C (CO)–C (CO) Bond Cleavage for C–N Bond Formation: One-Pot Domino Transformation of 1, 2-Diketones and Amidines into Imides and Amides CS Digwal, U Yadav, PVS Ramya, S Sana, B Swain, A Kamal The Journal of Organic Chemistry 82 (14), 7332-7345 , 2017 2017 Citations: 32
Hexafluoroisopropanol as a bioconjugation medium of ultrafast, tryptophan-selective catalysis M Nuruzzaman, BM Colella, CP Uzoewulu, AE Meo, EJ Gross, S Ishizawa, ... Journal of the American Chemical Society 146 (10), 6773-6783 , 2024 2024 Citations: 28
Synthesis and biological evaluation of novel imidazo [1, 2-a] pyridine-oxadiazole hybrids as anti-proliferative agents: Study of microtubule polymerization inhibition and DNA … DK Sigalapalli, G Kiranmai, GP Devi, R Tokala, S Sana, C Tripura, ... Bioorganic & Medicinal Chemistry 43, 116277 , 2021 2021 Citations: 26
Synthesis and preclinical evaluation of indole triazole conjugates as microtubule targeting agents that are effective against MCF-7 breast cancer cell lines V Yele, SKSS Pindiprolu, S Sana, D Ramamurty, JRK Madasi, ... Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal … , 2021 2021 Citations: 21
Regioselective ortho ‐sulfonamidation: Exploration of Intrinsic Directing Property of β‐Carbolines and their Photophysical Studies D Bora, S Reddy Dannarm, S Elza John, S Sana, R Sonti, N Shankaraiah Asian Journal of Organic Chemistry 10 (12), 3384-3389 , 2021 2021 Citations: 17
Sustainable photocatalytic C–H annulation of heteroarenes with sulfoxonium ylides: synthesis and photophysical properties of fused imidazo [1, 2-a] pyridine-based molecules S Sana, SR Dannarm, R Tokala, S Dastari, M Sathish, R Kumar, R Sonti, ... Organic Chemistry Frontiers 10 (19), 4800-4808 , 2023 2023 Citations: 15
Ring-opening cyclization of activated spiro-aziridine oxindoles with heteroarenes: a facile synthetic approach to spiro-oxindole-fused pyrroloindolines S Bhandari, S Sana, V Lahoti, R Tokala, N Shankaraiah RSC advances 10 (27), 16101-16109 , 2020 2020 Citations: 14
Visible-light-mediated photocatalytic sequential N-arylation: an eco-friendly synthetic route to unsymmetrical diarylamines and the imatinib drug S Sana, S Dastari, DS Reddy, R Tokala, M Sathish, R Sonti, ... Organic Chemistry Frontiers 10 (18), 4573-4580 , 2023 2023 Citations: 7
Synthesis and Evaluation of Bispecific 64Cu—Radio conjugates for PET Imaging in Prostate Cancer Models S Sana, X Ma, W Zhao, S Cheng, X Wu, X Ni, Z Wu, Z Li Journal of Nuclear Medicine 66 (supplement 1), 252187-252187 , 2025 2025
Synthesis and Evaluation of FAPI-Targeted 64Cu— Radioconjugates Containing Variable PEG Linkers for PET Imaging in U87 Cancer Models S Sana, X Ma, K Rakesh, W Zhao, Z Wu, Z Li Journal of Nuclear Medicine 65 (supplement 2), 242489-242489 , 2024 2024