Sanjay Sahay
@snggpgc.in
Professor of Botany
Sarojini Naidu Government Girls PG College, Bhopal
RESEARCH, TEACHING, or OTHER INTERESTS
Plant Science, General Biochemistry, Genetics and Molecular Biology, Catalysis, Renewable Energy, Sustainability and the Environment
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Vinod Kumar Chaurse and Sanjay Sahay
Elsevier BV
Tejpal Singh Parmar, Rajkumar Ahirwar, and Sanjay Sahay
Elsevier BV
Mangesh Kumar Mankar, U.S. Sharma, and Sanjay Sahay
Walter de Gruyter GmbH
Summary The declining productivity of little millet (Panicum sumatrense) in a rural area in India necessitates finding a sustainable solution. A suitable composition of plant growth-promoting rhizobacteria (PGPR) in the rhizosphere of a crop is considered important for its optimum yield. Therefore, a study was undertaken to isolate and identify suitable bacteria and see their priming effect on the productivity of little millet. Rhizospheric soil samples were used to isolate bacteria on nitrogen-free Jensen's medium. Fast-growing rhizobacterial isolates FKK5 and DUM4, which exhibit significant selected plant growth-promoting activities, were selected. Little millet seeds were inoculated with selected PGPR and a non-native Azotobacter chroococcum before sowing. The inoculated plants were grown under semi-sterile poly-house conditions. Little millet inoculated with FKK5, DUM4, and A. chroococcum (5576) showed enhanced grain yield by 28.14%, 24.72%, and 20.43%, respectively, and enhanced total biomass yield by 23.08%, 21.87%, and 19.09%, respectively. The isolates were identified as Burkholderia sp. with 0.66% dissimilarity in 16S rDNA sequence with the most closely related species in the Genbank database. The native PGPR proved to be more effective compared to non-native PGPR as biofertilizers in restoring the productivity of the nutritionally important cereal.
Kanak Choudhary, Najeeb Hussain Wani, Farooq Ahmad Ahanger, Suhaib Mohamad Malik, Vinod Chourse, Abdul Majid Khan, and Sanjay Sahay
Springer Nature Singapore
Sanjay Sahay
Springer Nature Singapore
Sanjay Sahay
Springer Nature Singapore
Tuyelee Das, Abdel Rahman Al-Tawaha, Devendra Kumar Pandey, Potshangbam Nongdam, Mahipal S. Shekhawat, Abhijit Dey, Kanak Choudhary, and Sanjay Sahay
Springer Nature Singapore
Sangita Chouhan, Rajkumar Ahirwar, Tejpal Singh Parmar, Ashiq Magrey, and Sanjay Sahay
Springer Nature Singapore
Kanak Choudhary, Mangesh Kumar Mankar, and Sanjay Sahay
Springer Nature Singapore
Sanjay Sahay
Elsevier
Mario Motto and Sanjay Sahay
Elsevier
Handbook ON Biofuels, José Guillermo León Ecopetrol, Raúl Gasparini, José Estrada Petroperu and Ernani Filgueiras
Elsevier
Sanjay Sahay
Elsevier
Mangesh Kumar Mankar, U.S. Sharma, and Sanjay Sahay
Walter de Gruyter GmbH
Abstract Azotobacter chroococcum is a universally accepted plant growth-promoting rhizospheric bacterium, which, as a biofertilizer, helps to increase the nitrogen level, solubilize the unavailable form of phosphorus, ensure growth-promoting metabolites, and control pathogenic microbes in the soil. A good strain of plant growth-promoting rhizobacteria (PGPR) needs to be produced, formulated, transported, stored, and distributed to the agriculture field. During all these operations, bacterial inoculants are transferred via a carrier material. One of the important challenges in biofertilizer technology is to ensure stability of the bacteria in the carrier. The study aimed to assess a novel carrier Lantana charcoal (LC; obtained from Lantana camara biomass), as compared to some currently available carriers. LC exhibited higher carbon content, low N2 content, neutral pH, and, above all, higher water-holding capacity, making it a suitable carrier material for A. chroococcum and possibly other PGPR. As a carrier, it showed no contamination during storage, exhibited the highest moisture content and moderate culture holding coefficient, and supported the highest colony-forming units per gram at the end of the storage period. Thus, LC cannot only serve as a better carrier, but its large-scale application would also ensure a reasonable use of this weed.
Sanjay Sahay, Vinod Chaurse, and Deepak Chauhan
Springer Science and Business Media LLC
Anilkumar A Dawande and Sanjay Sahay
Slovak University of Agriculture in Nitra
Concerted efforts have been made to push up andrographolide production through optimization of culture conditions, calli induction, cell growth and elicitation in cell suspension culture of Andrographis paniculata Nees. Optimum callus induction was obtained with cotyledon and hypocotyls of the plant on Skoog and Hilderbrandt (SH) medium containing 2.0 µg/mL 2,4- dichlorophenoxyacetic acid and 0.1µg/mL 6-benzyl amino purine (BAP). Half MS medium containing 20 g/L sucrose and 20 h photoperiod showed highest cells fresh weight (CFW) (17.96 ± 0.06 g/50 mL), growth index (10.95 ± 0.96) and andrographolide yield (4.61±0.688 mg/g DCW). The addition of copper sulphate (500 µM/L), Methyl jasmonate (25 mg/L), Chitin (500 mg/L) or Fungal mycelium (500 mg/L) in separate experiments showed significant increase (p=0.05) in bioproduction of andrographolide to the extent of 29.42±0.31 mg/g DCW, 13.13±0.11mg/g DCW, 19.45±0.68 mg/g DCW and 13.629±1.12 mg/g DCW respectively, copper sulphate thus proved to be the most effective one. The study indicated that a holistic approach involving both culture conditions and elicitation could enhance the overall production of secondary metabolites (andrographolide) appreciably in this herb and possibly in other medicinal plants.
Sanjay Sahay
Springer Science and Business Media LLC
Sanjay Sahay and Deepak Chouhan
Elsevier BV
Sanjay Sahay
Elsevier
Sanjay Sahay
Oriental Scientific Publishing Company
Twenty nine isolates of black yeasts isolated from different environmental sources such as soil, water and different plant parts (diseased wood, leaf, flower and fruit). Their preferable niche has been found to be leaves followed by other plant parts. They were less frequent in other ecosystem studied. This is the first report about the ecological survey of black yeast in Bhopal.
S. Sahay, B. Hamid, P. Singh, K. Ranjan, D. Chauhan, R.S. Rana, and V.K. Chaurse
Oxford University Press (OUP)
Of the twenty‐three morphotypes of yeasts isolated from soil capable of utilizing pectin as sole carbon source at 6°C, two yeast isolates, one psychrotolerant (PT1) and one psychrophilic (SPY11), were selected according to their ability to secrete pectinolytic enzymes under some oenological conditions (temperature 6 and 12°C and pH 3·5) and ability or inability to grow above 20°C, respectively. As compared to their optimal activity, the three pectinolytic enzymes viz., pectin methyl esterase (PME), endopolygalacturonase (endo‐PG) and exopolygalacturonase (exo‐PG) isolated and assayed at pH 3·5 from PT1 were found to retain 39, 60 and 60% activity at 12°C and 40, 79 and 74% activity at 28°C, respectively. Likewise, the enzymes PME and endo‐PG at pH 3·5 from SPY11 displayed 46 and 86% activity at 12°C and 50 and 60% activity at 28°C, respectively. All these enzymes showed 20–90% of residual activity at pH 3·5 and 6°C. The yeast isolates PT1 and SPY11 were identified as Rhodotorula mucilaginosa and Cystofilobasidium capitatum, respectively, on the basis of morphological, physiological and molecular characteristics. This study presents the first report on pectinolytic activities under major oenological conditions from psychrotolerant isolate R. mucilaginosa PT1 and psychrophilic isolate C. capitatum SPY11.