Shila Kumari Singh
@adtu.in
Assam down town University
RESEARCH, TEACHING, or OTHER INTERESTS
Microbiology (medical), Immunology
Scopus Publications
Scopus Publications
ShilaKumari Singh, Minakshi Bhattacharjee, Balagopalan Unni, and RajpalSingh Kashyap
Medknow
Background: Methicillin-resistant staphylococcus aureus (MRSA) are responsible for many hospital-acquired infections. Clindamycin (CL) is used to treat methicillin-sensitive S. aureus (MSSA) and MRSA. Antibiotic sensitivity testing (AST) can miss out the inducible CL resistance (CL-R) and result in failure of treatment. D-test detects inducible CL-R. Subjects and Methods: One hundred and ten S. aureus strains were tested. D-test was performed using erythromycin (ER) (15 mcg) and CL (2 mcg). The absence of inhibition around ER and a zone of inhibition around CL with flattening of the zone facing ER side is taken as positive D-test. Results: Of the total S. aureus strains, 36% were MRSA and 74% were MSSA. A total of 20 (18.18%) strains out of 110 were found to have inducible CL-R. In this study, MRSA (19.4%) were found to have higher percentage of D-test positivity as compared to MSSA (17.56%). Conclusion: D-test must be performed routinely as a part of AST for the presence of inducible phenotype.