PHYSICOCHEMICAL PROFILE AND BIOACTIVITY OF OLIGO-ULVAN FROM Ulva ohnoi SEAWEED DEPOLYMERIZED BY DIFFERENT PHYSICAL METHODS Caddoumy Zahrel Ben, Safrina Dyah Hardinigtyas, Wahyu Ramadhan Jurnal Pengolahan Hasil Perikanan Indonesia, 2026 Ulva ohnoi is a fast-growing green seaweed with high biomass productivity but remains underutilized beyond food and agricultural uses. Their main bioactive compound, ulvan, a sulfated polysaccharide, exhibits enhanced antioxidant and therapeutic activities when depolymerized into lower-molecular-weight forms. This study aimed to determine the effects of three physical depolymerization methods–ultrasound, ultraviolet (UV) radiation, and microwaves–on the physicochemical properties and bioactivity of ulvan extracted from Ulva ohnoi. Ulvan was extracted using hot maceration at 90°C for 2 h. The extracted ulvan was subjected to three treatments: ultrasonication for 60, 120, and 180 min; UV exposure for 60, 120, and 180 min; and microwave irradiation for 5, 10, and 15 min. Each treatment was performed in triplicates using a completely randomized design. The resulting oligo-ulvan was analyzed for molecular weight, viscosity, and bioactivities, including antioxidant activity (DPPH assay), α-glucosidase inhibition, and antiproliferative effects on fibroblast cells. Among the treatments, ultrasonication for 180 min produced oligo-ulvan with the lowest viscosity (1.41±0.06 cP) and molecular weight (78.2±2.5 kDa), along with significantly improved antioxidant activity (IC₅₀: 303.33 ppm), α-glucosidase inhibition (IC₅₀: 111.63±1.47 ppm), and antiproliferative effects (45.73±7.83% at 125 μg/mL). These findings highlight ultrasound-assisted depolymerization as a promising and environmentally friendly method for enhancing the therapeutic potential of ulvan for biomedical applications.
Modeling of Dynamic Material Behavior-Based Optimization of Gracilaria Powder, Kappa-Carrageenan, and Tricalcium Phosphate and Sequential Acidulant Adjustment for Instant Pre-Mix Seaweed Beverage Mochamad Alauddin Perdana Putra, Sri Purwaningsih, Wahyu Ramadhan Trends in Sciences, 2025 The growing demand for functional beverages has led to increased use of fiber-rich ingredients such as grains and nuts. However, the use of native seaweed as a primary ingredient in beverage formulations remains underreported. Its limited application is mainly due to processing challenges and poor compatibility with industrial requirements. Therefore, developing seaweed-based premixes offers a potential solution to enhance its usability in functional beverage products. This research aimed to optimize the formulation of an instant pre-mix powdered beverage based on Gracilaria using the I-Optimal Mixture Design, incorporating kappa-carrageenan as a stabilizer and tricalcium phosphate as an anti-caking agent and evaluated the effects of citric acid on the physical and functional characteristics of pre-mix powder. The independent variables included Gracilaria powder 36% - 45%, kappa-carrageenan 0% - 1%, and tricalcium phosphate 5% - 13%, while the measured responses encompassed moisture content, flowability, cohesiveness, flow time, angle of repose, and peak viscosity. The optimization results identified the optimal formulation as Gracilaria powder 36.52%, kappa-carrageenan 0.48%, and tricalcium phosphate 13%, yielding the highest desirability score of 0.973 with significant prediction confidence interval. To enhance solubility during the post-brewing process, citric acid was introduced as a chelating agent to improve solubility. Citric acid significantly improved the solubility of the post-brew powder, with a concentration of 4.5% producing the best CIE-L*a*b* ΔE value and solubility profile yielding a sedimentation index of 32.60% (close to the ideal < 30%), water absorption index of 2.63 g/g (> 2.2 g/g), water solubility index of 33.17%, and a ΔE value of 43.65. These findings emphasize the important role of citric acid in enhancing solubility and physical properties, thereby significantly improving the stability and overall quality of the product. This preliminary modeling of dynamic material behavior in instant powdered beverages offers a promising strategy for the food industry, supporting the demand for convenient and health-oriented products. HIGHLIGHTS Gracilaria seaweed powder presents a sustainable yet underutilized source of soluble fiber, offering significant potential for instant powdered beverage applications. The I-Optimal Mixture Design efficiently facilitates formulation optimization by elucidating critical physicofunctional parameters and component interactions within seaweed-based powders. Citric acid incorporation significantly improves post-brewing solubility, dispersion stability, and chromatic attributes, thereby enhancing the overall quality and consumer acceptability of the final product. GRAPHICAL ABSTRACT
Valorization of Pangasius byproduct oil in wax-structured oleogels as a potential butter substitute for reduced-fat chocolate-based products Wahyu Ramadhan, Fajar Domychen Sihombing, Bambang Riyanto, Sugeng Heri Suseno, Wini Trilaksani, Joko Santoso, Cahyuning Isnaini, Hana Fitria Navratilova, Muhamad Alif Razi, Neng Tanty Sofyana, Kyle Butler JAOCS Journal of the American Oil Chemists Society, 2025 Despite the extensive use of various vegetable oils in oleogel development, sustainable sources, such as belly Pangasius oil derived from industrial byproducts, offer a promising alternative as the liquid phase in oleogel systems. This research aimed to determine the optimal type and concentration of rice bran wax (RBW) and candelilla wax (CW) oleogelators based on Pangasius byproduct oil and to evaluate their application in producing reduced‐fat chocolate‐based products. The selected oleogel systems were further assessed, and the properties of the resulting chocolate products were characterized. The 5% CW oleogel exhibited superior gelling and oil binding capacity (99.86 ± 0.13%). Differential scanning calorimetry of oleogels with 2.5%, 5%, and 7.5% oleogelator concentrations showed a shift in melting points with increasing temperature. Rheological analysis revealed dynamic changes in G' and G" values as the oleogelator concentration increased. Application of the 5% CW oleogel yielded optimal results, producing chocolate products with physical and sensory characteristics comparable to those made with commercial butter. Substituting 50% of the butter with oleogel resulted in products with similar physical attributes and sensory acceptance. Hedonic testing showed no significant differences in most parameters across substitution levels (p > 0.05), with an average acceptance score exceeding 7 on a 9‐point scale. Notably, the total energy content of the chocolate product was reduced by 43.77% with 100% oleogel substitution. Eventually, this proof of concept highlights the potential of Pangasius byproduct oil as a sustainable liquid phase in oleogel technology, opening new possibilities for its application in confectionery.
Analysis quality fish oil (Decapterus sp.) containing omega-3 using extraction method (steam press) and (bligh and dyer) Advances in Environmental Biology, 2014
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