Changes in the seed and soil micobiota caused by seed treatment with chemical and biological agents Yurii Spychak, Tetiana Rozhkova, Liudmyla Tytova, Liudmyla Bilyuvska, Olha Bakumenko, Valentyna Tatarynova, Oleksandr Yemets, Viktor Demenko, Viktor Pivtoraiko, Alla Burdulaniuk Ecological Engineering and Environmental Technology, 2025 This study investigates the impact of chemical and biological treatments on the mycobiota of winter wheat seeds and the fungal composition of the soil.Conducted in 2023-2024 using the winter wheat variety Aliot, the research aimed to determine how different seed treatments affect the microbial populations and the development of wheat seedlings.The experiment was carried out in Sumy, Ukraine, at the Educational Research and Production complex of Sumy National Agrarian University.Chemical treatments tested included Tebuzan Ultra F.C.S., Celest Top 312.5 FS TH, Maxim 025 FS TH, and Record F.C.S., while biological agents included Azotobacterin-K BI, ECOSTERN Trichoderma CS, Bacillus megaterium, and others.Seeds were treated and their mycobiota were analyzed using biological methods to assess changes in fungal and bacterial populations.Additionally, treated seeds were sown to study the effects on the soil microbiota.The results showed that chemical treatments effectively suppressed several fungal species, particularly Alternaria tenuissima, and led to an increase in bacterial colonies within the seeds.However, they also reduced microbial diversity in the soil, which in some cases negatively impacted seedling development.In contrast, biological treatments, while less effective at completely suppressing fungal pathogens, enriched the seed microbiota, particularly increasing Aspergillus oryzae populations, and promoted the growth of beneficial soil microorganisms.Biological agents such as Azotobacterin-K BI and ECOSTERN Trichoderma CS significantly improved seedling length, reaching up to 13.59 cm, compared to chemical treatments which sometimes reduced seedling growth.Overall, the study highlights that while chemical treatments provide immediate protection against pathogens, biological treatments offer long-term benefits by enhancing microbial diversity and promoting healthier plant growth.These findings support the growing trend toward sustainable agriculture, where biological agents can be integrated to reduce chemical inputs and improve environmental safety without compromising yield.
Macroanalysis of Winter Wheat Seeds and Features of their Germination Tetiana Rozhkova, Alla Burdulanyuk, Valentyna Tatarynova, Oleksandr Yemets, Viktor Demenko, Yuriy Spychak, Viktor Pivtoraiko, Olha Bakumenko, Yelyzaveta Rozhkova Ecological Engineering and Environmental Technology, 2024 The peculiarities of the primary visual inspection of winter wheat seeds are considered. In the North-East of Ukraine, three groups of abnormal seeds (wrinkled, small seeds, and with a black point) were being identified by macroanalysis during 2017–2022. Factors affecting the representation of these groups in the samples were analyzed. It was determined that the amount of abnormal seeds is determined by the growing conditions of wheat, the place of cultivation and spraying of wheat with fungicides. The maximum number of substandard seeds was noted in Polissia, where black point dominated. In the Forest-steppe, the largest amount of such seeds was recorded in 2022, among which small seeds prevailed. A study of the characteristics of the manifestation of a black point showed that the blackening of the embryo occurs only from above. A seed with a black point is usually mature. For the first time, it was proven that spraying plants with essential oils of cinnamon and fennel leads to an increase in the appearance of the black point, which once again confirms its abiotic nature. The study of the germination of externally healthy and abnormal seeds of the Bohdana variety of the 2018 and 2019 harvests under conditions of low temperature and over moistening showed better indicators for the first group. But the germination of abnormal seeds had contradictory results, especially in different years. Comparison of the germination and development of seedlings from healthy and blackening seeds under favorable conditions during different periods of storage of wheat samples, from different places of cultivation, on different genotypes gave new unexpected results: higher indicators in the variant with black point. During further study in conditions of low temperature, which is explained by very late sowing of winter wheat in recent years in Ukraine, completely different data were obtained. Seed germination was better in healthy seeds. Seedlings developed faster at first also from healthy seeds, but on the last two dates of measurement, they began to grow faster from seeds with black point.
Interaction of endophytic fungi of winter wheat seeds Rozhkova, Tetiana, Biliavska, Liudmyla, Spychak, Yuriy Journal of Plant Protection Research, 2023 Seed endophytes are potential bioagents for plant protection and growth promoters.The question of the specifics of their isolation in cultural environments is not clear.The purpose of this study was to establish the nature of the interaction of endophytic fungi of wheat seeds with different levels of aggressiveness and presence in the mycobiota.Dual cultivation was carried out at potato-glucose agar (PGA), comparing with single fungal cultivation.The mutual influence of fungi during joint cultivation was established.Alternaria arborescens, which dominated in the mycobiota of wheat seeds from northeastern Ukraine, suppressed the development of only Penicillium.Nigrospora oryzae, Bipolaris sorokiniana, and Phoma developed faster than A. arborescens.Fusarium poae, and F. sporotrichioides competed for agar medium with N. oryzae.Known bioagents from wheat seeds showed unexpectedly low results.Trichothecium roseum formed a rejection zone during co-cultivation with F. graminearum.Trichoderma sp.Max18 (resistant to fludioxonil) on the 7th day inhibited the development of Penicillium, F. graminearum, and A. arborescens by 55, 48 and 26%, respectively.N. oryzae developed faster than the mycoparasitic fungus, but the latter began to parasitize it only from the 13th day.
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