Computer vision and machine-learning techniques for quantification and predictive modeling of intracellular anticancer drug delivery by nanocarriers Sanjay Goswami, Kshama D. Dhobale, Ravindra D. Wavhale, Barnali Goswami, Shashwat S. Banerjee Applied AI Letters, 2022 The field of cancer nanomedicine has made significant progress, but its clinical translation is impeded by many challenges, such as the difficulty in analyzing intracellular anticancer drug release by the nanocarriers due to the lack of suitable tools. Here, we propose the development of an image‐based strategy involving machine learning (ML) to evaluate anticancer drug such as doxorubicin hydrochloride (DOX) released by a nanocarrier inside the HCT116 colon cancer cells and its subsequent intracellular accumulation. This technique combines fluorescent cell imaging with ML‐based image analysis to assess and quantify the delivery of DOX by nanoparticles within them. We show that DOX in HCT116 cells was higher for multifunctional CNT‐DOX‐Fe3O4 nanocarrier than free DOX, indicating efficient and steady release of DOX as well as superior retentive property of the nanocarrier. Initially (1 and 4 hours), the luminance intensity of DOX in the cell cytoplasm delivered by CNT‐DOX‐Fe3O4 nanocarrier was ~0.34 and ~0.42 times lesser than that of free DOX delivered normally. However, at 24 and 48 hours posttreatment, the luminance intensity of DOX for CNT‐DOX‐Fe3O4 nanocarrier was ~1.98 and ~1.92 times higher than that of free DOX. Furthermore, the luminance intensity of DOX for CNT‐DOX‐Fe3O4 in the whole cell was ~1.35 and ~1.62 times higher than that of free DOX at 24 and 48 hours, respectively. The high‐throughput nature of our image analysis workflow allowed us to automate the process of DOX retention analysis and enabled us to devise ML‐based modeling to predict the percentage of anticancer drug retention in cells. The development of models to automatically quantify and predict intracellular drug release in cancer cells could benefit personalized treatments by optimizing the design of nanocarriers.
A quantitative precipitation forecast model using convective-cloud tracking in satellite thermal infrared images and adaptive regression: a case study along East Coast of India Barnali Goswami, Gupinath Bhandari, Sanjay Goswami Modeling Earth Systems and Environment, 2021 The current work addresses issues related to quantitative estimation of precipitation caused by convective clouds, using thermal infrared images, and adaptive regression modeling. The developed methodology has been implemented on the Indian sector during the period 22–25 October 2013. The importance of the developed methodology lies in the fact that the information obtained from it can facilitate further studies intended for the prediction of flood events. This study is the continuation of existing work of identification of convective clouds and the analysis of the Mesoscale Convective Systems (MCS). In the current work, forecast of rainfall in terms of millimeter has been proposed. The entire work has been carried out on thermal infrared (TIR) images obtained from geostationary satellites and the results have been validated by actual rainfall data measured by rain gauges. The results obtained from the developed methodology were found to be fairly close to actual values.
Mesoscale convective system tracking in satellite thermal infrared images Barnali Goswami, Gupinath Bhandari, Sanjay Goswami 11th IEEE India Conference Emerging Trends and Innovation in Technology Indicon 2014, 2015 In the field of meteorology, mesoscale convective systems are required to be distinguished, tracked, and their lifecycle to be observed, for precipitation forecasting. Mesoscale convective systems are easily identifiable from satellite infrared images. There are several existing techniques like temperature induced mean based cloud motion prediction model, for identification and tracking of cloud structures from a series of thermal infrared images. This technique has been previously implemented for tracking of single and then multiple cloud clusters. In this study, temperature induced mean based cloud motion prediction model has been deployed for tracking and predicting the motion of an entire convective system.
Temperature induced mean based cloud motion prediction model for multiple cloud clusters in satellite infrared images Barnali Goswami, Gupinath Bhandari, Sanjay Goswami Proceedings 4th International Conference on Emerging Applications of Information Technology Eait 2014, 2014 Thermal infrared images are quite useful in detection of convective clouds for meteorological purposes. Several techniques are available for the detection and tracking of clouds in satellite thermal infrared images. Temperature induced mean based cloud motion prediction model is one of them. But so far, it has been implemented for tracking single cloud cluster. In the present work this model is extended and applied for tracking of multiple cloud clusters so that it can be utilized for weather nowcasting.
Convective Cloud Detection and Tracking from Series of Infrared Images Barnali Goswami, Gupinath Bhandari Journal of the Indian Society of Remote Sensing, 2013 The most significant part of prediction of precipitation is the detection and identification of convective (cumulonimbus) clouds, also the tracking of cloud movement is important for identification of location of precipitation. A very simple methodology for detecting convective clouds and then tracking its movement from a series of infrared (IR) images is proposed in this paper. IR image is segmented using k-means clustering algorithm, which has been implemented using Euclidean, Manhattan and Mahalanobis distances and the results have been compared. Cloud clusters have been identified from segmented image and subsequently the large clusters were extracted. Center of Mass (CoM) was calculated for each selected cloud cluster and its position after every 30 min was predicted and compared with the actual values. If the predicted position deviates, the proposed models automatically adjusts itself, and the next prediction becomes closer to original values of position.
Development of irregular cloud cluster encapsulating structure from satellite infrared images 33rd Asian Conference on Remote Sensing 2012 Acrs 2012, 2012
Temperature induced mean based cloud motion prediction model from satellite infrared images Barnali Goswami, Gupinath Bhandari 2012 Annual IEEE India Conference Indicon 2012, 2012 Detection and identification of convective clouds can be done from thermal infrared (TIR) images (10.5-12.5μm) as clouds are associated with extremely low temperature. Clouds can be tracked from a given sequence of satellite images, and becomes useful for weather now-casting. There are several models available for cloud motion prediction. Adjusted Mean Based Prediction (AMBP) Model is one of them. A modified model of AMBP, based on temperature, for cloud movement tracking has been proposed and presented in this paper for better prediction.
Fuzzy min-max neural network for satellite infrared image clustering Barnali Goswami, Gupinath Bhandari, Sanjay Goswami Proceedings 2012 3rd International Conference on Emerging Applications of Information Technology Eait 2012, 2012 The process of estimation of precipitation from satellite images begins with the detection and identification of convective clouds. Clustering of the satellite infrared images is required in order to estimate the cloud cover area. In this paper a neuro-fuzzy technique in the form of unsupervised fuzzy minmax clustering neural (FMMCN) network has been implemented for clustering satellite infrared image. Each cluster is in the form of an n-dimensional hyperbox defined by minimum and maximum points and a fuzzy membership function. FMMCN suits this application area because it is completely unsupervised and hence, unlabeled data can be used with it. Also the number of clusters is not required to be mentioned at the beginning as it is calculated dynamically.
Automatically adjusting cloud movement prediction model from satellite infrared images Barnali Goswami, Gupinath Bhandari Proceedings 2011 Annual IEEE India Conference Engineering Sustainable Solutions Indicon 2011, 2011 Tracking and predicting cloud movement is one the most important step in pluviometry. The purpose of this study is to develop a self adjusting technique to predict the movement of clouds from a series of infrared (IR) images. The first task is to identify clouds from the images. This is done by clustering the images using K-means algorithm. Clouds are identified from segmented image and the large clusters are extracted. Center of Mass (CoM) is calculated for each cloud cluster and its position after every 30 minutes is predicted. Whenever the original position deviates from the predicted values, the model automatically adjusts itself with the change and the next prediction becomes closer to original values of position.
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