Keagile Bati

Scopus Publications

Flavonoids and phenols of Sclerocarya birrea fruit exocarp extracts differentially modulating antioxidant activities
Phazha Bushe Baeti, Keagile Bati, Phenyo Donald Brown, Runner Majinda, Kabo Masisi, Goabaone Gaobotse, Tebogo Elvis Kwape
Advances in Traditional Medicine, 2026
The aim of this study was to evaluate the total content of flavonoids and phenols, in vitro antioxidant and cytoprotective effects of Sclerocarya birrea fruit exocarp extracts. The fruit exocarp was extracted sequentially in Hexane, Chloroform, Ethyl acetate, and Methanol and the antioxidant properties were evaluated using in vitro antioxidant assays and cytoprotective effects against H2O2-induced cell death using HeLa cells. Molecular docking using Autodock 4.2.6 was used to assess the binding interactions of Sclerocarya birrea compounds to the antioxidant enzymes. The total flavonoid and total phenolic contents were in the order Methanol > Ethyl acetate > Chloroform > Hexane (P < 0.05) of Sclerocarya birrea fruit exocarp extracts. The antioxidant properties using DPPH, ABTS, and Ferric reducing antioxidant power, and total antioxidant capacity assays revealed significantly higher (P < 0.05) antioxidant activity in the Methanol > Ethyl acetate > Chloroform > Hexane. Using WST-1 assay, H2O2-induced cell loss was effectively reduced by preincubating HeLa cells with Ethyl acetate > Methanol > Chloroform > Hexane (P < 0.05) extracts. Methanol and Ethyl acetate extracts also significantly increased (P < 0.05) the activities of Superoxide dismutase, Catalase and reduced Glutathione. Amongst the identified compounds, Myricetin 3-o-α- rhamsoside (C8) emerged as the best compound to bind to both SOD and CAT with binding energies of − 9.1 and − 8.8 kcal/mol respectively. C8 formed hydrogen bonds and electrostatic interactions with SOD and CAT to act as an agonist. Our findings suggest that Methanol and Ethyl acetate Sclerocarya birrea fruit exocarp extracts improved antioxidant capacity and thus have the potential to be developed into antioxidant products that will alleviate oxidative stress linked diseases.
Synthesis, characterization, anticancer and antibacterial studies of ruthenium(II/III) benzimidazole complexes
Lebogang G. Julius, Moses O. Koobotse, Taye B. Demissie, James T.P. Matshwele, Florence M. Nareetsile, Tracy Molefe, Keagile Bati, David O. Nkwe, Daphne T. Mapolelo
Results in Chemistry, 2025
Benzimidazole derivatives are heterocyclic compounds that have diverse biological activities, including anticancer, antimicrobial and antiviral effects. In recent years, their coordination with versatile transition metals such as ruthenium (Ru) has gained significant interest in the development of metallodrugs. Herein, we report on the synthesis and characterization of ruthenium(II/IIII) benzimidazole based complexes namely, K[Ru(BBE)Cl 4 ], [Ru(BBE) 2 Cl 2 ] and [Ru(2-PC)(BBE)Cl]Cl, [where BBE = 1H-benzo[ d ]imidazol-2-yl ethane and 2-PC = 4-(4-nitrophenoxy)- N,N-bis (pyridin-2-ylmethyl)aniline]. The synthesized complexes and ligands were characterized using HRESI-MS, 1 H NMR, FTIR and UV–Vis spectroscopy. Density functional theory (DFT) calculations determined the geometry of the complexes. Biological studies assessed the complexes anticancer effects on MCF-7 breast cancer cells and their antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae . Anticancer studies showed that the complexes exhibited a concentration-dependent antiproliferative effect on MCF-7 breast cancer cells with IC 50 values ranging from 59.18 to 110.90 μM. To further investigate their anticancer potential, molecular docking studies were conducted in silico . The data revealed that the complexes interact with amino acid residues on the oestrogen receptor alpha (ER α ). The interaction energies ranged from −7.55 to −9.39 kcal/mol, compared to −6.09 kcal/mol for cisplatin. Antibacterial studies showed that [Ru(BBE) 2 Cl 2 ] had the highest antibacterial activity against S. aureus and E. faecalis , with inhibition zones of 11 mm and 14 mm, respectively. These findings highlight the potential of ruthenium benzimidazole complexes as promising next-generation metallodrugs with therapeutic applications that target both cancer and bacterial infections.
Extreme weather event attribution predicts climate policy support across the world
Viktoria Cologna, Simona Meiler, Chahan M. Kropf, Samuel Lüthi, Niels G. Mede, David N. Bresch, Oscar Lecuona, Sebastian Berger, John Besley, Cameron Brick, Marina Joubert, Edward W. Maibach, Sabina Mihelj, Naomi Oreskes, Mike S. Schäfer, Sander van der Linden, , Nor Izzatina Abdul Aziz, Suleiman Abdulsalam, Nurulaini Abu Shamsi, Balazs Aczel, Indro Adinugroho, Eleonora Alabrese, Alaa Aldoh, Mark Alfano, Innocent Mbulli Ali, Mohammed Alsobay, R. Michael Alvarez, Tabitha Amollo, Patrick Ansah, Denisa Apriliawati, Flavio Azevedo, Ani Bajrami, Ronita Bardhan, Keagile Bati, Eri Bertsou, Rahul Bhui, Olga Białobrzeska, Michal Bilewicz, Ayoub Bouguettaya, Katherine Breeden, Amélie Bret, Ondrej Buchel, Pablo Cabrera Alvarez, Federica Cagnoli, André Calero Valdez, Timothy Callaghan, Rizza Kaye Cases, Sami Çoksan, Gabriela Czarnek, Ramit Debnath, Sylvain Delouvée, Lucia Di Stefano, Celia Diaz-Catalàn, Kimberly C. Doell, Simone Dohle, Karen M. Douglas, Charlotte Dries, Dmitrii Dubrov, Malgorzata Dzimińska, Ullrich K. H. Ecker, Christian T. Elbaek, Mahmoud Elsherif, Benjamin Enke, Matthew Facciani, Antoinette Fage-Butler, Zaki Faisal, Xiaoli Fan, Christina Farhart, Christoph Feldhaus, Marinus Ferreira, Stefan Feuerriegel, Helen Fischer, Jana Freundt, Malte Friese, Albina Gallyamova, Patricia Garrido-Vásquez, Mauricio E. Garrido Vásquez, Olivier Genschow, Omid Ghasemi, Theofilos Gkinopoulos, Jamie L. Gloor, Ellen Goddard, Claudia González Brambila, Hazel Gordon, Dmitry Grigoryev, Lars Guenther, Håvard Haarstad, Dana Harari, Przemysław Hensel, Alma Cristal Hernández-Mondragón, Atar Herziger, Guanxiong Huang, Markus Huff, Mairéad Hurley, Nygmet Ibadildin, Mohammad Tarikul Islam, Tao Jin, Charlotte A. Jones, Sebastian Jungkunz, Dominika Jurgiel, Sarah Kavassalis, John R. Kerr, Mariana Kitsa, Tereza Klabíková Rábová, Olivier Klein, Hoyoun Koh, Aki Koivula, Lilian Kojan, Elizaveta Komyaginskaya, Laura M. König, Lina Koppel, Kochav Koren, Alexandra Kosachenko, John Kotcher, Laura S. Kranz, Pradeep Krishnan, Silje Kristiansen, André Krouwel, Toon Kuppens, Claus Lamm, Anthony Lantian, Aleksandra Lazić, Jean-Baptiste Légal, Zoe Leviston, Neil Levy, Amanda M. Lindkvist, Grégoire Lits, Andreas Löschel, Alberto López Ortega, Carlos Lopez-Villavicencio, Nigel Mantou Lou, Chloe H. Lucas, Kristin Lunz-Trujillo, Mathew D. Marques, Sabrina J. Mayer, Ryan McKay, Taciano L. Milfont, Joanne M. Miller, Panagiotis Mitkidis, Fredy Monge-Rodríguez, Matt Motta, Zarja Muršič, Jennifer Namutebi, Eryn J. Newman, Jonas P. Nitschke, Ntui-Njock Vincent Ntui, Daniel Nwogwugwu, Thomas Ostermann, Tobias Otterbring, Myrto Pantazi, Philip Pärnamets, Paolo Parra Saiani, Mariola Paruzel-Czachura, Michal Parzuchowski, Yuri G. Pavlov, Adam R. Pearson, Charlotte R. Pennington, Katerina Petkanopoulou, Marija B. Petrović, Dinara Pisareva, Adam Ploszaj, Ekaterina Pronizius, Karolína Pštross, Katarzyna Pypno-Blajda, Diwa Malaya A. Quiñones, Pekka Räsänen, Adrian Rauchfleisch, Felix G. Rebitschek, Gabriel Rêgo, James P. Reynolds, Joseph Roche, Jan Philipp Röer, Robert M. Ross, Isabelle Ruin, Osvaldo Santos, Ricardo R. Santos, Stefan Schulreich, Emily Shuckburgh, Johan Six, Nevin Solak, Leonhard Späth, Bram Spruyt, Samantha K. Stanley, Noel Strahm, Stylianos Syropoulos, Barnabas Szaszi, Ewa Szumowska, Mikihito Tanaka, Claudia Teran-Escobar, Boryana Todorova, Abdoul Kafid Toko, Renata Tokrri, Daniel Toribio-Florez, Manos Tsakiris, Michael Tyrala, Özden Melis Uluğ, Ijeoma Chinwe Uzoma, Jochem van Noord, Iris Vilares, Madalina Vlasceanu, Andreas von Bubnoff, Izabela Warwas, Tim Weninger, Mareike Westfal, Adrian Dominik Wojcik, Ziqian Xia, Jinliang Xie, Ewa Zegler-Poleska, Amber Zenklusen
Nature Climate Change, 2025
Extreme weather events are becoming more frequent and intense due to climate change. Yet, little is known about the relationship between exposure to extreme events, subjective attribution of these events to climate change, and climate policy support, especially in the Global South. Combining large-scale natural and social science data from 68 countries (N = 71,922), we develop a measure of exposed population to extreme weather events and investigate whether exposure to extreme weather and subjective attribution of extreme weather to climate change predict climate policy support. We find that most people support climate policies and link extreme weather events to climate change. Subjective attribution of extreme weather was positively associated with policy support for five widely discussed climate policies. However, exposure to most types of extreme weather event did not predict policy support. Overall, these results suggest that subjective attribution could facilitate climate policy support.
Trust in scientists and their role in society across 68 countries
Viktoria Cologna, Niels G. Mede, Sebastian Berger, John Besley, Cameron Brick, Marina Joubert, Edward W. Maibach, Sabina Mihelj, Naomi Oreskes, Mike S. Schäfer, Sander van der Linden, Nor Izzatina Abdul Aziz, Suleiman Abdulsalam, Nurulaini Abu Shamsi, Balazs Aczel, Indro Adinugroho, Eleonora Alabrese, Alaa Aldoh, Mark Alfano, Innocent Mbulli Ali, Mohammed Alsobay, Marlene Altenmüller, R. Michael Alvarez, Richard Amoako, Tabitha Amollo, Patrick Ansah, Denisa Apriliawati, Flavio Azevedo, Ani Bajrami, Ronita Bardhan, Keagile Bati, Eri Bertsou, Cornelia Betsch, Apurav Yash Bhatiya, Rahul Bhui, Olga Białobrzeska, Michał Bilewicz, Ayoub Bouguettaya, Katherine Breeden, Amélie Bret, Ondrej Buchel, Pablo Cabrera-Álvarez, Federica Cagnoli, André Calero Valdez, Timothy Callaghan, Rizza Kaye Cases, Sami Çoksan, Gabriela Czarnek, Steven De Peuter, Ramit Debnath, Sylvain Delouvée, Lucia Di Stefano, Celia Díaz-Catalán, Kimberly C. Doell, Simone Dohle, Karen M. Douglas, Charlotte Dries, Dmitrii Dubrov, Małgorzata Dzimińska, Ullrich K. H. Ecker, Christian T. Elbaek, Mahmoud Elsherif, Benjamin Enke, Tom W. Etienne, Matthew Facciani, Antoinette Fage-Butler, Md. Zaki Faisal, Xiaoli Fan, Christina Farhart, Christoph Feldhaus, Marinus Ferreira, Stefan Feuerriegel, Helen Fischer, Jana Freundt, Malte Friese, Simon Fuglsang, Albina Gallyamova, Patricia Garrido-Vásquez, Mauricio E. Garrido Vásquez, Winfred Gatua, Oliver Genschow, Omid Ghasemi, Theofilos Gkinopoulos, Jamie L. Gloor, Ellen Goddard, Mario Gollwitzer, Claudia González-Brambila, Hazel Gordon, Dmitry Grigoryev, Gina M. Grimshaw, Lars Guenther, Håvard Haarstad, Dana Harari, Lelia N. Hawkins, Przemysław Hensel, Alma Cristal Hernández-Mondragón, Atar Herziger, Guanxiong Huang, Markus Huff, Mairéad Hurley, Nygmet Ibadildin, Maho Ishibashi, Mohammad Tarikul Islam, Younes Jeddi, Tao Jin, Charlotte A. Jones, Sebastian Jungkunz, Dominika Jurgiel, Zhangir Kabdulkair, Jo-Ju Kao, Sarah Kavassalis, John R. Kerr, Mariana Kitsa, Tereza Klabíková Rábová, Olivier Klein, Hoyoun Koh, Aki Koivula, Lilian Kojan, Elizaveta Komyaginskaya, Laura König, Lina Koppel, Kochav Koren Nobre Cavalcante, Alexandra Kosachenko, John Kotcher, Laura S. Kranz, Pradeep Krishnan, Silje Kristiansen, André Krouwel, Toon Kuppens, Eleni A. Kyza, Claus Lamm, Anthony Lantian, Aleksandra Lazić, Oscar Lecuona, Jean-Baptiste Légal, Zoe Leviston, Neil Levy, Amanda M. Lindkvist, Grégoire Lits, Andreas Löschel, Alberto López Ortega, Carlos Lopez-Villavicencio, Nigel Mantou Lou, Chloe H. Lucas, Kristin Lunz-Trujillo, Mathew D. Marques, Sabrina J. Mayer, Ryan McKay, Hugo Mercier, Julia Metag, Taciano L. Milfont, Joanne M. Miller, Panagiotis Mitkidis, Fredy Monge-Rodríguez, Matt Motta, Iryna Mudra, Zarja Muršič, Jennifer Namutebi, Eryn J. Newman, Jonas P. Nitschke, Ntui-Njock Vincent Ntui, Daniel Nwogwugwu, Thomas Ostermann, Tobias Otterbring, Jaime Palmer-Hague, Myrto Pantazi, Philip Pärnamets, Paolo Parra Saiani, Mariola Paruzel-Czachura, Michal Parzuchowski, Yuri G. Pavlov, Adam R. Pearson, Myron A. Penner, Charlotte R. Pennington, Katerina Petkanopoulou, Marija B. Petrović, Jan Pfänder, Dinara Pisareva, Adam Ploszaj, Karolína Poliaková, Ekaterina Pronizius, Katarzyna Pypno-Blajda, Diwa Malaya A. Quiñones, Pekka Räsänen, Adrian Rauchfleisch, Felix G. Rebitschek, Cintia Refojo Seronero, Gabriel Rêgo, James P. Reynolds, Joseph Roche, Simone Rödder, Jan Philipp Röer, Robert M. Ross, Isabelle Ruin, Osvaldo Santos, Ricardo R. Santos, Philipp Schmid, Stefan Schulreich, Bermond Scoggins, Amena Sharaf, Justin Sheria Nfundiko, Emily Shuckburgh, Johan Six, Nevin Solak, Leonhard Späth, Bram Spruyt, Olivier Standaert, Samantha K. Stanley, Gert Storms, Noel Strahm, Stylianos Syropoulos, Barnabas Szaszi, Ewa Szumowska, Mikihito Tanaka, Claudia Teran-Escobar, Boryana Todorova, Abdoul Kafid Toko, Renata Tokrri, Daniel Toribio-Florez, Manos Tsakiris, Michael Tyrala, Özden Melis Uluğ, Ijeoma Chinwe Uzoma, Jochem van Noord, Christiana Varda, Steven Verheyen, Iris Vilares, Madalina Vlasceanu, Andreas von Bubnoff, Iain Walker, Izabela Warwas, Marcel Weber, Tim Weninger, Mareike Westfal, Florian Wintterlin, Adrian Dominik Wojcik, Ziqian Xia, Jinliang Xie, Ewa Zegler-Poleska, Amber Zenklusen, Rolf A. Zwaan
Nature Human Behaviour, 2025
Science is crucial for evidence-based decision-making. Public trust in scientists can help decision makers act on the basis of the best available evidence, especially during crises. However, in recent years the epistemic authority of science has been challenged, causing concerns about low public trust in scientists. We interrogated these concerns with a preregistered 68-country survey of 71,922 respondents and found that in most countries, most people trust scientists and agree that scientists should engage more in society and policymaking. We found variations between and within countries, which we explain with individual- and country-level variables, including political orientation. While there is no widespread lack of trust in scientists, we cannot discount the concern that lack of trust in scientists by even a small minority may affect considerations of scientific evidence in policymaking. These findings have implications for scientists and policymakers seeking to maintain and increase trust in scientists.
Perceptions of science, science communication, and climate change attitudes in 68 countries - the TISP dataset
Niels G. Mede, Viktoria Cologna, Sebastian Berger, John Besley, Cameron Brick, M. Joubert, E. Maibach, Sabina Mihelj, Naomi Oreskes, Mike S. Schäfer, Sander L. van der Linden, N Izzatina Abdul Aziz, Suleiman Abdulsalam, Nurulaini Abu Shamsi, B. Aczel, Indro Adinugroho, Eleonora Alabrese, Alaa Aldoh, Mark Alfano, I. M. Ali, Mohammed Alsobay, M. Altenmüller, R. M. Alvarez, Richard Amoako, Tabitha A. Amollo, Patrick Ansah, Denisa Apriliawati, Flavio Azevedo, Ani Bajrami, R. Bardhan, Keagile Bati, Eri Bertsou, C. Betsch, Apurav Yash Bhatiya, Rahul Bhui, Olga Białobrzeska, Michał Bilewicz, A. Bouguettaya, Katherine Breeden, A. Bret, Ondrej Buchel, Pablo Cabrera-Álvarez, Federica Cagnoli, André Calero Valdez, T. Callaghan, Rizza Kaye C. Cases, Sami Çoksan, G. Czarnek, Steven De Peuter, Ramit Debnath, Sylvain Delouvée, Lucia Di Stefano, Celia Díaz-Catalán, Kimberly C. Doell, Simone Dohle, Karen M. Douglas, Charlotte Dries, D. Dubrov, Małgorzata Dzimińska, Ullrich K. H. Ecker, Christian T. Elbaek, Mahmoud Elsherif, Benjamin Enke, Tom Etienne, M. Facciani, A. Fage-Butler, Md Zaki Faisal, Xiaoli Fan, Christina E. Farhart, Christoph Feldhaus, Marinus Ferreira, Stefan Feuerriegel, Helen Fischer, Jana Freundt, Malte Friese, Simon Fuglsang, Albina Gallyamova, Patricia Garrido-Vásquez, Mauricio E. Garrido Vásquez, Winfred Gatua, Oliver Genschow, Omid Ghasemi, T. Gkinopoulos, Jamie L Gloor, Ellen Goddard, Mario Gollwitzer, C. González-Brambila, Hazel Gordon, Dmitry Grigoryev, G. Grimshaw, Lars Guenther, H. Haarstad, Dana Harari, L. Hawkins, P. Hensel, A. C. Hernández-Mondragón, Atar Herziger, Guanxiong Huang, Markus Huff, Mairéad Hurley, N. Ibadildin, Maho Ishibashi, Mohammad Tarikul Islam, Younes Jeddi, Tao Jin, Charlotte A Jones, Sebastian Jungkunz, Dominika Jurgiel, Zhangir Kabdulkair, Johanna Kao, Sarah Kavassalis, J. Kerr, M. Kitsa, Tereza Klabíková Rábová, Olivier Klein, Hoyoun Koh, A. Koivula, Lilian Kojan, Elizaveta Komyaginskaya, L. König, L. Koppel, Kochav Koren Nobre Cavalcante, A. Kosachenko, J. Kotcher, Laura S Kranz, Pradeep Krishnan, Silje Kristiansen, André Krouwel, T. Kuppens, E. Kyza, Claus Lamm, A. Lantian, A. Lazić, Óscar Lecuona, Jean-Baptiste Légal, Zoe Leviston, Neil L Levy, Amanda M Lindkvist, G. Lits, A. Löschel, Alberto López-Ortega, Carlos Lopez-Villavicencio, N. Lou, C. Lucas, Kristin Lunz-Trujillo, Mathew D. Marques, Sabrina J Mayer, Ryan McKay, Hugo Mercier, Julia Metag, T. Milfont, Joanne M Miller, P. Mitkidis, Fredy S. Monge-Rodriguez, M. Motta, I. Mudra, Zarja Muršič, Jennifer Namutebi, Eryn J. Newman, Jonas P. Nitschke, Ntui-Njock Vincent Ntui, D. Nwogwugwu, T. Ostermann, T. Otterbring, Jaime Palmer-Hague, Myrto Pantazi, P. Pärnamets, Paolo Parra Saiani, M. Paruzel-Czachura, Michał Parzuchowski, Y. G. Pavlov, Adam R. Pearson, M. Penner, Charlotte R. Pennington, Katerina Petkanopoulou, Marija M Petrović, Jan Pfänder, Dinara Pisareva, Adam Płoszaj, Karolína Poliaková, Ekaterina Pronizius, Katarzyna Pypno-Blajda, D. M. Quiñones, Pekka Räsänen, Adrian Rauchfleisch, F. G. Rebitschek, Cintia Refojo Seronero, Gabriel Gaudencio Rego, James P Reynolds, Joseph Roche, Simone Rödder, J. P. Röer, R. Ross, Isabelle Ruin, Osvaldo Santos, Ricardo R Santos, P. Schmid, Stefan Schulreich, B. Scoggins, Amena Sharaf, Justin Sheria Nfundiko, Emily Shuckburgh, Johan Six, Nevin Solak, Leonhard Späth, B. Spruyt, Olivier Standaert, Samantha K. Stanley, G. Storms, Noel Strahm, Stylianos Syropoulos, Barnabas Szaszi, E. Szumowska, Mikihito Tanaka, Claudia Teran-Escobar, Boryana Todorova, Abdoul Kafid Toko, Renata Tokrri, Daniel Toribio-Flórez, M. Tsakiris, M. Tyrala, Ö. Ulug, I. Uzoma, Jochem van Noord, C. Varda, Steven Verheyen, I. Vilares, Madalina Vlasceanu, Andreas von Bubnoff, Iain Walker, I. Warwas, Marcel Weber, Tim Weninger, Mareike Westfal, Florian Wintterlin, A. Wójcik, Ziqian Xia, Jinliang Xie, Ewa Zegler-Poleska, Amber Zenklusen, R. A. Zwaan
Scientific Data, 2025
Science is integral to society because it can inform individual, government, corporate, and civil society decision-making on issues such as public health, new technologies or climate change. Yet, public distrust and populist sentiment challenge the relationship between science and society. To help researchers analyse the science-society nexus across different geographical and cultural contexts, we undertook a cross-sectional population survey resulting in a dataset of 71,922 participants in 68 countries. The data were collected between November 2022 and August 2023 as part of the global Many Labs study "Trust in Science and Science-Related Populism" (TISP). The questionnaire contained comprehensive measures for individuals' trust in scientists, science-related populist attitudes, perceptions of the role of science in society, science media use and communication behaviour, attitudes to climate change and support for environmental policies, personality traits, political and religious views and demographic characteristics. Here, we describe the dataset, survey materials and psychometric properties of key variables. We encourage researchers to use this unique dataset for global comparative analyses on public perceptions of science and its role in society and policy-making.
Euclea natalensis Suppresses Postprandial Hyperglycemia in Rats via the Inhibition of α-Glucosidase: In vitro, in vivo, and Molecular Docking Studies
Keagile Bati, Runner R.T Majinda, Goabaone Gaobotse, Tebogo E. Kwape
Current Traditional Medicine, 2025
Background: Postprandial hyperglycemia is a key factor in type 2 diabetes, and its management is critical in alleviating the deleterious consequences of diabetes and its associated micro and macrovascular complications. Objective: The current study aims to determine the effect of Euclea natalensis leaf extracts on α- glucosidase inhibition in vitro and postprandial hyperglycemia in vivo in rats. Methods: Sequentially extracted leaf extracts of Euclea natalensis were evaluated for their inhibitory effects on α-glucosidase in vitro and the suppression of postprandial hyperglycemia in normoglycemic rats. The extracts were fingerprinted using a Fourier-transform infrared spectrophotometer (FTIR), and the bioactive compounds were evaluated by molecular docking for their interaction with α-glucosidase. Results: FTIR fingerprinting of the extracts showed that they contain functional groups of important bioactive phytochemicals. The extracts inhibited α-glucosidase in vitro, with the methanol extract (1 mg/mL) showing the highest inhibitory effect of 93.52 ± 1.50% compared to 69.62% ± 1.45 of the standard drug acarbose (0.05 mg/mL). The extracts also reduced postprandial hyperglycemia in rats in a sucrose tolerance test, where the hexane and methanol extracts performed similarly to Acarbose. Molecular docking studies showed that 20(29)-lupene-3β- isoferulate 3 is the most potent α-glucosidase inhibitor with the lowest binding energy of -10.79 kcal/mol, 2 hydrogen bonds with residues ASP1526 and ASP1157, and numerous Van der Waal interactions with amino acids in the binding pocket of α-glucosidase. Conclusion: Euclea natalensis leaf extracts were found to suppress postprandial hyperglycemia by inhibiting α-glucosidase activity; thus, it has a promising potential for use as an antidiabetic agent.
Pro-oxidant Effects of Repeatedly Heated Sunflower Oil and the Possible Ameliorative Potential of Elaeodendron transvaalense Leaf Powder in Sprague Dawley Rats
Tebogo Kwape, Kabo Masisi, Laone Kelaotswe, Keagile Bati, Phazha Baeti
Current Traditional Medicine, 2025
Background: Repeated heating of cooking oils induces the overproduction of reactive oxygen species with an overwhelmed cellular antioxidant defense system, resulting in oxidative stress, the known cause of cardiovascular diseases. Elaeodendron transvaalense is a medicinal plant believed to have phytochemicals that help ameliorate the effects of oxidative stress. Objective: This study investigated the pro-oxidant effects of repeatedly heated sunflower oil and the possible ameliorative potential of Elaeodendron transvaalense leaf powder in Sprague Dawley rats. Methods: Four groups of male Sprague Dawley rats were fed for 4-weeks a standard diet supplemented without (a) control or with (b) 15% (w/w) repeatedly heated sunflower oil (RHSO), (c) 15% (w/w) non-heated sunflower oil, (d) 15% (w/w) RHSO and 1.5% ETLP. Results: Endogenous antioxidants: Catalase and SOD activities were observed to rise significantly (p <0.05) in Group 2 when compared to Group 1 and Group 3 respectively. A significant decline in the SOD and catalase activities was observed in group 4 fed RHSO + ETLP. No significant differences were observed in the body, liver, and kidney weights of Group 2 when compared with Groups 1, 3 and 4 respectively, and no significant differences were observed when comparing Group 4 to Groups 1, 2 and 3. The levels of Lipid profiles; Low-density Lipoproteins (LDL), Total cholesterol (TC), and Triglycerides (TG) were observed to rise significantly (p <0.05) in Group 2 when compared to Group 1 and 3, while their significant (p <0.05) decline was observed in Group 4 fed with RHSO + ETLP. The levels of HDL showed no significant difference across all groups because the p-values for the difference between groups exceeded the null hypothesis (p <0.05). Conclusion: The findings of this experiment indicate the possible ameliorative potential of ETLP observed in the significantly (p <0.05) reduced SOD and catalase activities. A significant (p <0.05) decline in the TC, TG, and LDL to desirable levels further indicated the ameliorative effects.
Sclerocarya birrea and Terminalia prunioides: Phytochemical screening and synergistic inhibition of cervical cancer cells proliferation through modulation of EGFR, VEGF, MACC1, CYFRA 21-1, and CD 95 gene expressions
Phazha Bushe Baeti, Donald Phenyo Brown, Keagile Bati, G.F. Chi, Ibrahim Demirtaş, Kabo Masisi, Goabaone Gaobotse, Tebogo Elvis Kwape
South African Journal of Botany, 2024
In vitro α-amylase and α-glucosidase inhibitory effects and antioxidant potential of new dihydrochalcones from Baphia massaiensis Taub
Nayang A. Kgakatsi, Runner R.T. Majinda, Ishmael B. Masesane, Mutshinyalo S. Nwamadi, Taye B. Demissie, Keagile Bati
Phytochemistry Letters, 2024
Phytochemical screening, antioxidant, and cytotoxic activities of sequentially extracted Euclea natalensis leaf extracts
Keagile Bati, Phazha B. Baeti, Nayang A. Kgakatsi, Runner R.T. Majinda, Goabaone Gaobotse, Tebogo E. Kwape
Scientific African, 2024
Oxidative stress plays a major role in the pathogenesis of many diseases like diabetes, cancer, cardiovascular diseases, and neurodegenerative diseases. Oxidative stress has become a potential for therapeutic target in the prevention and management of such diseases. This has spiked the interest in the use of natural antioxidants for disease prevention. The present study was aimed at assessing the antioxidant and cytotoxic properties of sequentially extracted Euclea natalensis leaf extracts. The total phenolics and flavonoids were estimated, and antioxidant activity was determined using 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), total antioxidant capacity, and the ferric reducing/antioxidant power (FRAP) assays. The cytotoxic capacity against HeLa cells was evaluated using WST-1 (4-[3-(4-Iodophenyl)-2-(4-nitro-phenyl)-2H-5-tetrazolio]-1,3-benzene sulfonate) colorimetric assay. Qualitative phytochemical screening showed the presence of phytochemicals such as phenols, flavonoids, tannins, terpenoids and glycosides except in the hexane extract. The total phenolic and flavonoid content ranged from 0.58–118.7 mg GAE/g DW and 9.8–20.1 mg GAE/g DW respectively. All extracts displayed a concentration-dependent inhibition of DPPH and ABTS, with IC50 values in the range 0.04–0.122 mg/mL and 0.079–0.205 mg/mL respectively. The aqueous extract had the highest total antioxidant capacity and ferric reducing power of 40.4 mg AAE/g DW and 0.98 mg Vitamin C equivalence/g DW. The IC50 value for cytotoxicity was in the range 155.3–211.3 mg/mL with the dichloromethane-methanol extracts being the least cytotoxic. It was concluded that Euclea natalensis leaf extracts contain bioactive phytochemicals with antioxidant potential and less cytotoxic capacity.
Cobalt pyridyl complexes against drug-resistant bacteria: Synthesis, characterization, antibacterial properties, mode of action, and molecular docking studies
James T.P. Matshwele, Sebusi Odisitse, Ofentse Mazimba, Taye B. Demissie, Moses O. Koobotse, Daphne T. Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Mosimanegape Jongman, Florence M. Nareetsile
Inorganica Chimica Acta, 2024
Evaluation of the α-amylase inhibitory activity of Euclea natalensis extracts used in the treatment of diabetes mellitus: An experimental and in silico approach
Keagile Bati, Phazha Bushe Baeti, Goabaone Gaobotse, Tebogo E. Kwape
Plant Science Today, 2024
Leaf extracts of Euclea natalensis A.D.C ameliorate biochemical abnormalities in high-fat-low streptozotocin-induced diabetic rats through modulation of the AMPK-GLUT4 pathway
Keagile Bati, Phazha Bushe Baeti, Goabaone Gaobotse, Tebogo Elvis Kwape
Egyptian Journal of Basic and Applied Sciences, 2024
Synthesis, characterization, antibacterial properties, mode of action and molecular docking studies of copper pyridyl complexes against drug-resistant bacteria
James T.P. Matshwele, Sebusi Odisitse, Taye B. Demissie, Moses O. Koobotse, Ofentse Mazimba, Daphne Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Mosimanegape Jongman, Florence Nareetsile
Inorganic Chemistry Communications, 2023
Reduction of Bacterial Folic Acid Production and Cell Membrane Disruption of Klebsiella pneumoniae by Two Amino Substituted Pyridyl Compounds: An Experimental and In Silico Approach
James T. P. Matshwele, Mosimanegape Jongman, Taye B. Demissie, Moses O. Koobotse, Ofentse Mazimba, Daphne Mapolelo, Keagile Bati, Lebogang G. Julius, David O. Nkwe, Florence Nareetsile, Sebusi Odisitse
Chemistry Africa, 2023
Antioxidant and cytoprotective effects of sequentially extracted Terminalia prunioides pods
Phazha Baeti, Keagile Bati, Kabo Masisi, Goabaone Gaobotse, Tebogo Kwape
Egyptian Journal of Basic and Applied Sciences, 2023
Anti-diabetic effects of an ethanol extract of Cassia abbreviata stem bark on diabetic rats and possible mechanism of its action: Anti-diabetic properties of Cassia abbreviata
Keagile Bati, Tebogo Elvis Kwape, Padmaja Chaturvedi
Journal of Pharmacopuncture, 2017

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