Sandra Regina Georgetti

@uel.br

Professor in Department of Pharmaceutical Sciences/State University of Londrina
State University of Londrina

Sandra Regina Georgetti


            Download Compact PDF  
https://researchid.co/sandrageorgetti

RESEARCH INTERESTS

DEvelopement of topical formulations added natural antioxidant to prevent or delay UVB-induced skin damage
76

Scopus Publications

Scopus Publications

  • Resolvin D2 Reduces UVB Skin Pathology by Targeting Cytokines, Oxidative Stress, and NF-κB Activation
    Ingrid C. Pinto, Priscila Saito, Camilla C. A. Rodrigues, Renata M. Martinez, Cristina P. B. Melo, et al.
    Antioxidants, 2025
    UVB skin pathology is initiated by reactive oxygen species (ROS), differentiating this condition from other inflammatory diseases involving first the immune cell activation by danger or pathogen molecular patterns followed by oxidative stress. Resolvin D2 (RvD2) has been found to reduce inflammation in preclinical models. However, whether or not RvD2 reduces skin pathology caused by UVB irradiation is not yet known. Therefore, the efficacy of RvD2 on skin pathology triggered by UVB irradiation in female hairless mice was assessed. RvD2 (0.3, 1 or 3 ng/mouse, i.p.) was found to protect the skin against UVB inflammation, as observed in the reduction in edema (46%), myeloperoxidase activity (77%), metalloproteinase-9 activity (39%), recruitment of neutrophils/macrophages (lysozyme+ cells, 76%) and mast cells (106%), epidermal thickening (93%), sunburn cell formation (68%), collagen fiber breakdown (55%), and production of cytokines such as TNF-α (100%). Considering the relevance of oxidative stress to UVB irradiation skin pathologies, an important observation was that the skin antioxidant capacity was recovered by RvD2 according to the results that show the ferric reducing antioxidant power (68%), cationic radical scavenges (93%), catalase activity (74%), and the levels of reduced glutathione (48%). Oxidative damage was also attenuated, as observed in the reduction in superoxide anion production (69%) and lipid hydroperoxides (71%). The RvD2 mechanism involved the inhibition of NF-κB activation, as observed in the diminished degradation of IκBα (48%) coupled with a reduction in its downstream targets that are involved in inflammation and oxidative stress, such as COX-2 (66%) and gp91phox (77%) mRNA expression. In conclusion, RvD2 mitigates the inflammatory and oxidative pathologic skin aggression that is triggered by UVB.
  • Patches containing quercetin microcapsules to ameliorate dermal herpes simplex virus injuries in mice
    Jéssica Bassetto Carra, Maria Laura Goussain Darido, Camila Felix Vecchi, Mariana Carla de Oliveira, Ricardo Luís Nascimento de Matos, et al.
    European Journal of Pharmaceutics and Biopharmaceutics, 2025
  • Bioadhesive Polymeric Films Containing Rhamnolipids, An Innovative Antimicrobial Topical Formulation
    Jéssica B. Carra, Kamila B. B. Wessel, Giovana N. Pereira, Mariana C. Oliveira, Pietra M. T. Pattini, et al.
    AAPS Pharmscitech, 2024
    Acne affects most of the world's population, causing an impact on the self-esteem of adolescents and young adults. One of the causes is the presence of the bacteria Cutibacterium acnes which are part of the natural microbiota of the skin. Topical treatments consist of anti-inflammatory and antibiotics, which could select resistant strains. Alternatives to the antibiotic are biocomposites that have antimicrobial activity like biosurfactants which are produced by bacteria. An innovative way of applying these compounds is bioadhesive polymeric films that adhere to the skin and release the active principle topically. Rhamnolipids have great potential to be used in the treatment of acne because they present antimicrobial activity against C. acnes in low and safe concentrations (MIC of 15.62 µg/mL, CBM of 31.25 µg/mL and CC50 of 181.93 µg/mL). Four films with different rhamnolipids concentrations (0.0; 0.1; 0.2; and 0.3%, w/w) were obtained as to visual appearance, mass variation, thickness, density, solubility, pH, water vapor transmission, mechanical properties (folding endurance, bioadhesion strength, tensile strength, elongation at break and Young's modulus), scanning electron microscopy and infrared. The results show that these formulations had a homogeneous appearance; elastic mechanical properties; pH similar to human skin and bioadhesive. The polymeric films containing rhamnolipids were effective against C. acnes, in the in vitro test, at the three concentrations tested, the film with the highest concentration (0.3%, w/w) being the most promising for presenting the highest antimicrobial activity. Thus, the polymeric film containing rhamnolipids has the potential to be used in the treatment of acne.
  • Resolvin D5 Protects Female Hairless Mouse Skin from Pathological Alterations Caused by UVB Irradiation
    Priscila Saito, Ingrid C. Pinto, Camilla C. A. Rodrigues, Ricardo L. N. de Matos, David L. Vale, et al.
    Antioxidants, 2024
    Resolvin D5 (RvD5) is a lipid mediator that has been reported to present anti-inflammatory and pro-resolution properties. Evidence also supports its capability to enhance reactive oxygen species (ROS) production during bacterial infections, which would be detrimental in diseases driven by ROS. The biological activity of RvD5 and mechanisms against UVB irradiation skin pathology have not been investigated so far. Female hairless mice were treated intraperitoneally with RvD5 before UVB stimulus. RvD5 reduced skin edema in a dose-dependent manner as well as oxidative stress by increasing antioxidants (endogenous tissue antioxidant scavenging of cationic radical, iron reduction, catalase activity and reduced glutathione levels) and decreasing pro-oxidants (superoxide anion and lipid peroxidation). RvD5 antioxidant activity was accompanied by enhancement of Nrf2, HO-1 and NQO1 mRNA expression. RvD5 reduced the production of IL-1β, TNF-α, TGF-β, and IL-10. RvD5 also reduced the inflammatory cell counts, including mast cells and neutrophils/macrophages. The reduction of oxidative stress and inflammation resulted in diminished matrix metalloproteinase 9 activity, collagen degradation, epidermal thickening and sunburn cell development. Therefore, this study demonstrates, to our knowledge, the first body of evidence that RvD5 can be used to treat UVB skin pathology and unveils, at least in part, its mechanisms of action.
  • Development of trans-Chalcone loaded pectin/casein biodegradable microcapsules: Efficacy improvement in the management of experimental colitis
    Carla F.S. Guazelli, Victor Fattori, Barbara B. Colombo, Isabela S. Ludwig, Laisa G. Vicente, et al.
    International Journal of Pharmaceutics, 2023
  • Aspirin-Triggered Resolvin D1 (AT-RvD1) Protects Mouse Skin against UVB-Induced Inflammation and Oxidative Stress
    Cristina P. B. Melo, Priscila Saito, Renata M. Martinez, Larissa Staurengo-Ferrari, Ingrid C. Pinto, et al.
    Molecules, 2023
    Intense exposure to UVB radiation incites excessive production of reactive oxygen species (ROS) and inflammation. The resolution of inflammation is an active process orchestrated by a family of lipid molecules that includes AT-RvD1, a specialized proresolving lipid mediator (SPM). AT-RvD1 is derived from omega-3, which presents anti-inflammatory activity and reduces oxidative stress markers. The present work aims to investigate the protective effect of AT-RvD1 on UVB-induced inflammation and oxidative stress in hairless mice. Animals were first treated with 30, 100, and 300 pg/animal AT-RvD1 (i.v.) and then exposed to UVB (4.14 J/cm2). The results showed that 300 pg/animal of AT-RvD1 could restrict skin edema, neutrophil and mast cell infiltration, COX-2 mRNA expression, cytokine release, and MMP-9 activity and restore skin antioxidant capacity as per FRAP and ABTS assays and control O2•− production, lipoperoxidation, epidermal thickening, and sunburn cells development. AT-RvD1 could reverse the UVB-induced downregulation of Nrf2 and its downstream targets GSH, catalase, and NOQ-1. Our results suggest that by upregulating the Nrf2 pathway, AT-RvD1 promotes the expression of ARE genes, restoring the skin’s natural antioxidant defense against UVB exposition to avoid oxidative stress, inflammation, and tissue damage.
  • Fermented (By Monascus purpureus or Aspergillus oryzae) and Non-Fermented Defatted Soybean Flour Extracts: Biological Insight and Mechanism Differences in Inflammatory Pain and Peritonitis
    Marília F. Manchope, Mariana M. Bertozzi, Sergio M. Borghi, Cíntia L. Handa, Mariana A. Queiroz-Cancian, et al.
    Fermentation, 2023
    Background: Monascus purpureus and Aspergillus oryzae have been used to ferment defatted soybean flour (DSF: DSFF-Mp and DSSF-Ao, respectively) extract, improving antioxidant availability and conversion of the glycosylated isoflavones to aglycones. The aim of the present study was to evaluate the biological activity of fermented and non-fermented DSF extracts in pain and inflammation, which has not yet been explored. Methods: Phenolic compounds of extracts were determined. Non-fermented DSF (DSF-Non), DSFF-Mp, and DSFF-Ao (10–100 mg/kg) were administrated i.p., 30 min before i.pl. or i.p. carrageenan stimulus. Mechanical and thermal hyperalgesia, edema, histopathology, leukocyte recruitment, and oxidative stress in the paw tissue, and inflammatory cell recruitment, NFκB activation, and cytokine production were assessed in the peritoneum. Stomach and kidney toxicity were evaluated. Results: DSF-Non, DSFF-Mp, and DSFF-Ao extracts inhibited mechanical and thermal hyperalgesia, paw edema, histopathology, neutrophil recruitment, and oxidative stress, as well as inhibited peritoneal leukocyte recruitment. DSF-Non increased IL-10, and DSFF-Ao reduced IL-33 levels. DSFF-Mp increased IL-10 and reduced IL-33 production, and NFκB activation in CD45+ cells, without inducing toxicity. Conclusions: The present data reveal for the first time that fermented/non-fermented DSF extracts are analgesic and anti-inflammatory, showing differences in the mechanism of action depending on fungi applied for fermentation.
  • Therapeutic Potential of Controlled Delivery Systems in Asthma: Preclinical Development of Flavonoid-Based Treatments
    Sergio M. Borghi, Tiago H. Zaninelli, Jéssica B. Carra, Olivia K. Heintz, Marcela M. Baracat, et al.
    Pharmaceutics, 2023
    Asthma is a chronic disease with increasing prevalence and incidence, manifested by allergic inflammatory reactions, and is life-threatening for patients with severe disease. Repetitive challenges with the allergens and limitation of treatment efficacy greatly dampens successful management of asthma. The adverse events related to several drugs currently used, such as corticosteroids and β-agonists, and the low rigorous adherence to preconized protocols likely compromises a more assertive therapy. Flavonoids represent a class of natural compounds with extraordinary antioxidant and anti-inflammatory properties, with their potential benefits already demonstrated for several diseases, including asthma. Advanced technology has been used in the pharmaceutical field to improve the efficacy and safety of drugs. Notably, there is also an increasing interest for the application of these techniques using natural products as active molecules. Flavones, flavonols, flavanones, and chalcones are examples of flavonoid compounds that were tested in controlled delivery systems for asthma treatment, and which achieved better treatment results in comparison to their free forms. This review aims to provide a comprehensive understanding of the development of novel controlled delivery systems to enhance the therapeutic potential of flavonoids as active molecules for asthma treatment.
  • Spray-drying of casein/pectin bioconjugate microcapsules containing grape (Vitis labrusca) by-product extract
    Jéssica Bassetto Carra, Ricardo Luís Nascimento de Matos, Ana Paula Novelli, Renê Oliveira do Couto, Fabio Yamashita, et al.
    Food Chemistry, 2022
    Novel microcapsules containing grape peel by-product extract were obtained. In this pursuit, complex coacervation of casein/pectin bioconjugate and spray-drying were combined. We have investigated the role of the dispersion feed rate (FR), drying air inlet temperature (IT) and drying air flow rate (AR) in the drying yield, microencapsulation efficiency, total polyphenols and anthocyanins contents, antioxidant activity, and morphology of the products. Also, the first-order degradation kinetics of the phytochemicals for both the extract and dried microcapsules was assessed and compared. The loss on the phytochemicals during spray-drying was attenuated in up to 88%, and the IT was the main factor affecting the particle properties. The polyphenols on the extract interacted with the polymers, influencing the assemble of the bioconjugate and the particle's features. Such microencapsulation strategy enhanced the thermal stability of the phytochemicals and rendered biocompatible and biodegradable products of which the nutraceutical and cosmeceutical application may have potential.
  • Pimenta pseudocaryophyllus (Gomes) Landrum extract inhibits inflammatory pain in mice: targeting neutrophil recruitment, oxidative stress, and cytokine production
    Marília F. Manchope, Sandra S. Mizokami, Camila R. Ferraz, Sergio M. Borghi, Josiane A. Vignoli, et al.
    Natural Product Research, 2022
    Pimenta pseudocaryophyllus (Gomes) Landrum is a Brazilian native plant. The mechanisms by which it promotes analgesia are unknown. We demonstrated the analgesic effect of P. pseudocaryophyllus dried extract (3 mg/kg; i.p.) in the following models of inflammatory pain (maximal inhibition): phenyl-p-benzoquinone (89%), formalin (72% - 1st phase and 96% - 2nd phase for flinches, and 50% - 1st phase and 71% - 2nd phase for licking behavior), complete Freund's adjuvant (95% - flinches and 33% - licking behavior), and carrageenin (56% - mechanical and 85% - thermal hyperalgesia) without motor impairment. Its analgesic effect depends on inhibiting neutrophil recruitment (95% - histopathology, 83% - myeloperoxidase activity, and 80% - LysM-eGFP mice), oxidative stress (86% - GSH and 98% - superoxide anion), and cytokine production (35% - IL-33, 80% - TNF-α, and 95% - IL-1β). The present study advances in understanding the analgesic mechanisms of P. pseudocaryophyllus.
  • Hydroethanolic Extract of Grape Peel from Vitis labrusca Winemaking Waste: Antinociceptive and Anti-Inflammatory Activities
    Cristiana F. G. Silva, Victor Fattori, Caroline R. Tonetti, Marcos A. S. Ribeiro, Ricardo L. N. Matos, et al.
    Food Technology and Biotechnology, 2022
  • Rosmarinus officinalis extract-loaded emulgel prevents UVB irradiation damage to the skin
    KÁTIA S. TAKAYAMA, MARIANA C. MONTEIRO, PRISCILA SAITO, INGRID C. PINTO, CLAUDIA T. NAKANO, et al.
    Anais Da Academia Brasileira De Ciencias, 2022
  • Protection against UVB deleterious skin effects in a mouse model: effect of a topical emulsion containing Cordia verbenacea extract
    Cristina P. B. Melo, Priscila Saito, David L. Vale, Camilla C. A. Rodrigues, Ingrid C. Pinto, et al.
    Photochemical and Photobiological Sciences, 2021
  • Protective effect of oral treatment with Cordia verbenacea extract against UVB irradiation deleterious effects in the skin of hairless mouse
    Cristina P.B. Melo, Priscila Saito, David L. Vale, Camilla C.A. Rodrigues, Ingrid C. Pinto, et al.
    Journal of Photochemistry and Photobiology B Biology, 2021
  • A topical formulation containing quercetin-loaded microcapsules protects against oxidative and inflammatory skin alterations triggered by UVB irradiation: enhancement of activity by microencapsulation
    David L. Vale, Renata M. Martinez, Daniela C. Medeiros, Camila da Rocha, Natália Sfeir, et al.
    Journal of Drug Targeting, 2021
  • Topical Administration of 15-Deoxy-Δ12,14-Prostaglandin J2 Using a Nonionic Cream: Effect on UVB-Induced Skin Oxidative, Inflammatory, and Histopathological Modifications in Mice
    Clovis M. Kumagai, Renata M. Martinez, Barbara B. Colombo, Priscila Saito, Ingrid C. Pinto, et al.
    Mediators of Inflammation, 2021
  • Analgesic activity and mechanism of action of a Beta vulgaris dye enriched in betalains in inflammatory models in mice
    Renata M. Martinez, Miriam S. Hohmann, Daniela T. Longhi-Balbinot, Ana C. Zarpelon, Marcela M. Baracat, et al.
    Inflammopharmacology, 2020
  • The lipoxin receptor/FPR2 agonist BML-111 protects mouse skin against ultraviolet B radiation
    Renata M. Martinez, Victor Fattori, Priscila Saito, Ingrid C. Pinto, Camilla C. A. Rodrigues, et al.
    Molecules, 2020
  • Prevention of uvb radiation-induced oxidative stress in mice by topical administration of azadirachta indica (Neem) extract
    Renata Micheli Martinez, Clara Ruiza de Souza, Cristina de Paula Barros de Melo, Marcela Maria Baracat, Nilton Syogo Arakawa, et al.
    Revista De Ciencias Farmaceuticas Basica E Aplicada, 2020
  • Treatment with maresin 1, a docosahexaenoic acid-derived pro-resolution lipid, protects skin from inflammation and oxidative stress caused by UVB irradiation
    Talita L. C. Cezar, Renata M. Martinez, Camila da Rocha, Cristina P. B. Melo, David L. Vale, et al.
    Scientific Reports, 2019
  • Parameters of the fermentation of soybean flour by Monascus purpureus or Aspergillus oryzae on the production of bioactive compounds and antioxidant activity
    Cíntia Ladeira Handa, Fernando Sanches de Lima, Marcela Fernanda Geton Guelfi, Meg da Silva Fernandes, Sandra Regina Georgetti, et al.
    Food Chemistry, 2019
  • Preclinical Evaluation of Rutin-Loaded Microparticles with an Enhanced Analgesic Effect
    Daniela Cristina de Medeiros, Sandra Satie Mizokami, Natalia Sfeir, Sandra Regina Georgetti, Alexandre Urbano, et al.
    ACS Omega, 2019
  • Production of hydrolysate of okara protein concentrate with high antioxidant capacity and aglycone isoflavone content
    Vitória Ribeiro Garcia de Figueiredo, Ariana Justus, Dafne Garcia Pereira, Sandra Regina Georgetti, Elza Iouko Ida, et al.
    Brazilian Archives of Biology and Technology, 2019
  • Validation of methodology for assay, pharmaceutical equivalence, and comparative dissolution profile for tablets containing amlodipine besylate
    R. M. Martinez, Jenifer Freitas da Silva, L. R. Jorge, Rhye Lessa Ishikawa, Ana Paula Novelli, et al.
    Journal of Applied Pharmaceutical Science, 2019
  • Topical emulsion containing pyrrolidine dithiocarbamate: effectiveness against ultraviolet B irradiation-induced injury of hairless mouse skin
    Renata M. Martinez, Ana L. M. Ivan, David L. Vale, Marcela Z. Campanini, Vitor S. Ferreira, et al.
    Journal of Pharmacy and Pharmacology, 2018