Size Characteristics and Mechanisms of Toxic Action of Selenium, Cobalt and Silver Nanoparticles on Mitochondria of the Liver of Rats S. S. Anufrick, A. I. Savko, S. N. Anuchin, T. A. Kovalenya, T. V. Ilyich, T. K. Krupskaya, E. A. Lapshina, I. B. Zavodnik Biophysics Russian Federation, 2025 Nanosized materials are widely used in biomedical nanotechnologies, but the mechanisms of toxic effects of metal and nonmetal nanoclusters remain unclear. The aim of this work is to evaluate the size characteristics and mechanisms of toxic action of silver, selenium and cobalt nanoparticles at the level of isolated mitochondria. Using the laser ablation method, silver (rounded, ~10–20 and ~50 nm), cobalt (cubic and prismatic, ~100–200 nm) and selenium (spherical, ~20–30 and 135–180 nm) nanoparticles with different spectral characteristics and capable of forming conglomerates were obtained. Silver, cobalt, and selenium nanoparticles (0.1–10 μg/mL) effectively inhibited the respiratory activity of isolated rat liver mitochondria by disrupting the coupling of oxidation and phosphorylation, which was accompanied by a drop in the mitochondrial membrane potential. The uncoupling effect of nanoparticles may be associated with the transfer of electrons from the electron transport chain of mitochondria to the positively charged surface of nanoparticles and depends on the size and material of the nanoparticles.
Radiation Divergence of Microsecond Rhodamine 6G Solution Lasers and Solid-State Perylene-Activated Nanocomposite Laser V. V. Tarkovskii, S. S. Anufrik, A. O. Romashkevich, P. R. Makei Bulletin of the Lebedev Physics Institute, 2022 Abstract The radiation divergences of a microsecond laser on ethanol solution of rhodamine 6G and perylene-activated solid-state laser on nanoporous glass–polymere (NPGP) composite are compared, depending on the specific energy of pumping radiation. It is established that the main reasons of large radiation divergence of the NPGP nanocomposite laser are the light scattering of the pumping radiation and laser radiation by the heterogeneous medium of the composite and the thermal self-action.
An Excimer XeCl Minilaser S. S. Anufrick, A. P. Volodenkov, K. F. Znosko, V. F. Losev Atmospheric and Oceanic Optics, 2022 An electric-discharge XeCl minilaser and the results of its experimental modeling are described. The laser uses a two-stage system of magnetic compression of current pulses, semiconductor high-voltage bipolar transistors as a switch, and preionization of the working medium by barrier discharge radiation from under the grid electrode. The dependence of the output parameters of the minilaser on the excitation conditions and the composition of the active medium is derived. The maximal pulse energy of ∼12 mJ was obtained for a mixture 1.5 Torr HCl + 15 Torr Xe + 4 atm Ne at a voltage across the peaking capacitor of 20 kV and a lasing pulse duration at half maximum of ∼20 ns. The efficiency of the XeCl minilaser in terms of the energy stored in the sharpening capacitor is ∼4%.
Copper and Selenium Nanoparticles as a New Tool against Antibiotic-Resistant Pathogenic Microorganisms Р.И. Довнар, С.М. Смотрин, С.С. Ануфрик, С.Н. Анучин, И.С. Довнар, Н.Н. Иоскевич Surgery Eastern Europe, 2022 Введение. Все более возрастающая антибиотикорезистентность патогенных микроорганизмов является тревожным знаком современной хирургии. Наличие устойчивости к антибиотикам удлиняет сроки госпитализации пациентов, увеличивает заболеваемость и смертность, повышает затраты на здравоохранение. Так, в Республике Беларусь ежегодные потери вследствие наличия антибактериальной резистентности микроорганизмов составляют от 93,5 до 131,5 млн белорусских рублей.Цель. Изучение количественных характеристик антибактериального действия наночастиц меди и селена по отношению к клиническим полиантибиотикорезистентным штаммам грамположительных и грамотрицательных бактерий.Материалы и методы. Противомикробное действие наночастиц меди и селена исследовалось на двух штаммах грамположительных бактерий – Staphylococcus aureus и Staphylococcus haemolyticus, а также пяти штаммах грамотрицательных бактерий – Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis и Pseudomonas aeruginosa. Антибактериальные свойства данных наночастиц изучались с помощью метода серийных разведений в жидкой питательной среде. В качестве способа синтеза наночастиц меди и селена был использован метод лазерной абляции в жидкости.Результаты. Проведенные исследования продемонстрировали, что наночастицы меди и селена обладают выраженным противомикробным действием по отношению к клиническим патогенным штаммам как грамположительных, так и грамотрицательных микроорганизмов. Учитывая наличие полиантибиотикорезистентности у данных штаммов, применение наночастиц исследованных элементов является перспективным направлением в хирургии, особенно в случае гнойных процессов, вызванных полирезистентной, смешанной или трудно поддающейся лечению микрофлорой.Выводы. Наночастицы меди и селена являются уникальным классом веществ, проявляющих выраженную антибактериальную эффективность в отношении полиантибиотикорезистентной микрофлоры, что показывает возможность их широкого применения в различных областях хирургии в будущем. Introduction. Increasing antibiotic resistance of pathogenic microorganisms is an alarming sign of modern surgery. The presence of antibiotic resistance lengthens hospital stays, increases morbidity and mortality, and increases healthcare costs. Thus, in the Republic of Belarus, annual losses due to the presence of antibacterial resistance of microorganisms range from 93.5 to 131.5 million Belarusian rubles annually.Purpose. To study quantitative characteristics of antibacterial action of copper and selenium nanoparticles against clinical polyantibiotic-resistant strains of gram-positive and gram-negative bacteria.Materials and methods. The antimicrobial effect of copper and selenium nanoparticles was studied on two gram-positive strains: Staphylococcus aureus and Staphylococcus haemolyticus and five strains of gram-negative bacteria: Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa. The antibacterial properties of these nanoparticles were studied using the method of serial dilutions in a liquid nutrient medium. The laser ablation in liquid was used as a method of synthesizing copper and selenium nanoparticles.Results. The conducted studies have demonstrated that copper and selenium nanoparticles exhibit a pronounced antimicrobial effect against clinical pathogenic strains of both gram-positive and gram-negative microorganisms. Given the presence of polyantibiotic resistance in these strains, the use of nanoparticles of the studied elements is a promising direction in surgery, especially in case of purulent processes caused by multiresistant, mixed or difficult to treat microflora.Conclusions. Copper and selenium nanoparticles represent the unique class of substances exhibiting pronounced antibacterial efficacy against polyantibiotic-resistant microflora, which suggests the possibility of their wide application in various fields of surgery in the future.
Effect of pump-induced loss on the spectral dependence of Rhodamine 6G laser efficiency under microsecond coherent pumping V.V. Tarkovsky, S.S. Anufrik, V.Yu. Kurstak Quantum Electronics, 2021 We examine the effect of the pump-induced loss in the gain medium of a Rhodamine 6G laser on the spectral dependence of the laser efficiency under microsecond coherent pumping. It is shown theoretically and experimentally that the pump-induced loss is mainly due to nonlinear refraction and thermo-optic inhomogeneities of the gain medium, which produce a negative thermal lens, whose defocusing effect reduces the laser efficiency. The induced absorption by excited-state singlet and triplet levels of dye molecules is shown to make no significant contribution to changes in laser efficiency. The pump-induced thermal loss at the peak absorption wavelength of Rhodamine 6G plays a more important role well above the threshold pump power density (γ ⩾ 5). We demonstrate that the maximum dye laser efficiency under microsecond coherent pumping can only be achieved at an optimal pump wavelength.
Computer modeling of XeCl-lasers Slavomir Anufrick, Alexander Volodenkov, Kazimir Znosko, Valery Losev Proceedings of SPIE the International Society for Optical Engineering, 2021
XeCl-excilamp with the capacitance discharge S. S. Anufrik, A. P. Volodenkov, K. F. Znosko Conference Proceedings 5th International Conference on Advanced Optoelectronics and Lasers Caol 2010, 2010
Methods of XeCl-lasers computer modeling Slavomir S. Anufrik, Vasiliy O. Shkleinik, Alexander P. Volodenkov, Kazimir F. Znosko Proceedings of Caol 2008 4th International Conference on Advanced Optoelectronics and Lasers, 2008
Technique of XeCl-excilamps computer modeling Slavomir S. Anufri, Vasiliy O. Shkleinik, Alexander P. Volodenkov, Kazimir F. Znosko 2008 International Conference on Laser and Fiber Optical Networks Modeling Lfnm 2008, 2008
Technique of XeCl-excilamps computer modeling Slavomir S. Anufrik, Vasiliy O. Shkleinik, Alexander P. Volodenkov, Kazimir F. Znosko 2008 International Conference on Laser and Fiber Optical Networks Modeling Lfnm 2008, 2008
Some types of XeCl-excilamps Slavomir Anufrik, Alexander Volodenkov, Kazimir Znosko Proceedings of SPIE the International Society for Optical Engineering, 2008
Simulation of active medium of XeCl lasers Slavomir Anufrik, Alexander Volodenkov, Kazimir Znosko Proceedings of SPIE the International Society for Optical Engineering, 2007
Kinetic model for XeCL-lasers Slavomir Anufrik, Alexander Volodenkov, Kazimir Znosko 8th International Conference on Laser and Fiber Optical Networks Modeling Lfnm 2006, 2006
The simple excilamp on basis of XeCl-mixture S. Anufrik, A. Volodenkov, K. Znosko Proceedings of Caol 2005 2nd International Conference on Advanced Optoelectronics and Lasers, 2005
Compact electric-discharge excimer lasers Proceedings of Lfnm 2004 6th International Conference on Laser and Fiber Optical Networks Modeling, 2004
Simplified model for XeCl-lasers Proceedings of Lfnm 2004 6th International Conference on Laser and Fiber Optical Networks Modeling, 2004
Simulation of preionization system for XeCl-lasers Proceedings of Lfnm 2004 6th International Conference on Laser and Fiber Optical Networks Modeling, 2004