Cristian Bis Humbert

@neurocentre-magendie.fr

Postdoc/ Psychobiology of vulnerability to drug addiction
Inserm



              

https://researchid.co/cristianbis-humbert

RESEARCH, TEACHING, or OTHER INTERESTS

Physiology, Neuroscience, Pharmacology, Toxicology and Pharmaceutics, Neuropsychology and Physiological Psychology

12

Scopus Publications

Scopus Publications

  • Evaluating the daily modulation of FADD and related molecular markers in different brain regions in male rats
    Fernando Yáñez‐Gómez, Laura Gálvez‐Melero, Sandra Ledesma‐Corvi, Cristian Bis‐Humbert, Elena Hernández‐Hernández, Glòria Salort, Rubén García‐Cabrerizo, and M. Julia García‐Fuster
    Wiley
    AbstractFas‐Associated protein with Death Domain (FADD), a key molecule controlling cell fate by balancing apoptotic versus non‐apoptotic functions, is dysregulated in post‐mortem brains of subjects with psychopathologies, in animal models capturing certain aspects of these disorders, and by several pharmacological agents. Since persistent disruptions in normal functioning of daily rhythms are linked with these conditions, oscillations over time of key biomarkers, such as FADD, could play a crucial role in balancing the clinical outcome. Therefore, we characterized the 24‐h regulation of FADD (and linked molecular partners: p‐ERK/t‐ERK ratio, Cdk‐5, p35/p25, cell proliferation) in key brain regions for FADD regulation (prefrontal cortex, striatum, hippocampus). Samples were collected during Zeitgeber time (ZT) 2, ZT5, ZT8, ZT11, ZT14, ZT17, ZT20, and ZT23 (ZT0, lights‐on or inactive period; ZT12, lights‐off or active period). FADD showed similar daily fluctuations in all regions analyzed, with higher values during lights off, and opposite to p‐ERK/t‐ERK ratios regulation. Both Cdk‐5 and p35 remained stable and did not change across ZT. However, p25 increased during lights off, but exclusively in striatum. Finally, no 24‐h modulation was observed for hippocampal cell proliferation, although higher values were present during lights off. These results demonstrated a clear daily modulation of FADD in several key brain regions, with a more prominent regulation during the active time of rats, and suggested a key role for FADD, and molecular partners, in the normal physiological functioning of the brain's daily rhythmicity, which if disrupted might participate in the development of certain pathologies.

  • Evaluating signs of hippocampal neurotoxicity induced by a revisited paradigm of voluntary ethanol consumption in adult male and female Sprague-Dawley rats
    Carles Colom-Rocha, Cristian Bis-Humbert, and M. Julia García-Fuster
    Springer Science and Business Media LLC
    Abstract Background Binge alcohol drinking is considered a prominent risk factor for the development of alcohol-use disorders, and could be model in rodents through the standard two-bottle preference choice test. The goal was to recreate an intermittent use of alcohol during 3 consecutive days each week to ascertain its potential impact on hippocampal neurotoxicity (neurogenesis and other neuroplasticity markers), and including sex as a biological variable, given the well-known sex differences in alcohol consumption. Methods Ethanol access was granted to adult Sprague–Dawley rats for 3 consecutive days per week, followed by 4 days of withdrawal, during 6 weeks, mimicking the most common pattern of intake in people, drinking over the weekends in an intensive manner. Hippocampal samples were collected to evaluate signs of neurotoxicity. Results Female rats consumed significantly more ethanol than males, although intake did not escalate over time. Ethanol preference levels remained below 40% over time and did not differ between sexes. Moderate signs of ethanol neurotoxicity were observed in hippocampus at the level of decreased neuronal progenitors (NeuroD + cells), and these effects were independent of sex. No other signs of neurotoxicity were induced by ethanol voluntary consumption when measured through several key cell fate markers (i.e., FADD, Cyt c, Cdk5, NF-L) by western blot analysis. Conclusions Overall, the present results suggest that even though we modeled a situation where no escalation in ethanol intake occurred across time, mild signs of neurotoxicity emerged, suggesting that even the use of ethanol during adulthood in a recreational way could lead to certain brain harm.

  • Adolescent cocaine induced persistent negative affect in female rats exposed to early-life stress
    Cristian Bis-Humbert and M. Julia García-Fuster
    Springer Science and Business Media LLC
    Abstract Rationale The combination of several risk factors (sex, a prior underlying psychiatric condition, or early drug initiation) could induce the emergence of negative affect during cocaine abstinence and increase the risk of developing addiction. However, most prior preclinical studies have been centered in male rodents, traditionally excluding females from these analyses. Objectives To ascertain the behavioral and neurochemical consequences of adolescent cocaine exposure when the combination of several risk factors is present (female, early-life stress). Methods Whole litters of Sprague–Dawley rats were exposed to maternal deprivation for 24 h on postnatal day (PND) 9. Cocaine was administered in adolescence (15 mg/kg/day, i.p., PND 33–39). Negative affect was assessed by several behavioral tests (forced swim, open field, novelty-suppressed feeding, sucrose preference). Hippocampal cell fate markers were evaluated by western blot (FADD, Bax, cytochrome c) or immunohistochemistry (Ki-67; cell proliferation). Results Maternal deprivation is a suitable model of psychiatric vulnerability in which to study the impact of adolescent cocaine in female rats. While adolescent cocaine did not alter affective-like behavior during adolescence, a pro-depressive–like state emerged during adulthood, exclusively in rats re-exposed to cocaine during abstinence. FADD regulation by cocaine in early-life stressed female rats might contribute to certain hippocampal neuroadaptations with some significance to the observed induced negative affect. Conclusions Adolescent cocaine induced persistent negative affect in female rats exposed to early-life stress, highlighting the risk of early drug initiation during adolescence for the emergence of negative reinforcement during abstinence likely driving cocaine addiction vulnerability, also in female rats.

  • Dose-dependent opposite effects of nortriptyline on affective-like behavior in adolescent rats: Comparison with adult rats
    Cristian Bis-Humbert, Rubén García-Cabrerizo, and M. Julia García-Fuster
    Elsevier BV

  • Electroconvulsive seizures protect against methamphetamine-induced inhibition of neurogenesis in the rat hippocampus
    Rubén García-Cabrerizo, Cristian Bis-Humbert, and M. Julia García-Fuster
    Elsevier BV

  • Antidepressant-like effects of cannabidiol in a rat model of early-life stress with or without adolescent cocaine exposure
    Cristian Bis-Humbert, Rubén García-Cabrerizo, and M. Julia García-Fuster
    Springer Science and Business Media LLC

  • Increased negative affect when combining early-life maternal deprivation with adolescent, but not adult, cocaine exposure in male rats: regulation of hippocampal FADD
    Cristian Bis-Humbert, Rubén García-Cabrerizo, and M. Julia García-Fuster
    Springer Science and Business Media LLC


  • Decreased sensitivity in adolescent versus adult rats to the antidepressant-like effects of cannabidiol
    Cristian Bis-Humbert, Rubén García-Cabrerizo, and M. Julia García-Fuster
    Springer Science and Business Media LLC

  • Adolescent morphine induces emotional signs of withdrawal paired with neurotoxicity selectively in male rats: Female resilience
    Fernando Jiménez-Romero, Cristian Bis-Humbert, and M. Julia García-Fuster
    Elsevier BV