Andre Romero da Silva

@ifes.edu.br

Industrial Chemistry Department
Federal Institute of Espírito Santo

Andre Romero da Silva


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https://researchid.co/aromero
Graduate at Bachelor of Technological Chemical from Universidade Estadual de Campinas (2000), master's at Chemistry from Universidade Estadual de Campinas (2003) and Ph.D. at Chemistry from Universidade Estadual de Campinas (2007). Has experience in Chemistry, acting on the following subjects: indium meso-tetraphenylporphyrin, photodynamic therapy, photosensitizers, biomolecules photooxidation and mechanism of photooxidation. He has held positions as Chemistry course coordinator, general teaching coordinator, Teaching Director, Director of Research and Pos-Graduate and is the actual Pro-rector of Research and Pos-Graduate at Federal Institute of Espírito Santo.

EDUCATION

2017-2018 - Specialization in Pedagogical Practices by Federal Institute of Espírito Santo - Brazil
2003-2007 - Doctor of Science (Physical Chemistry) by State University of Campinas - Brazil
2001-2003 - Master in Chemistry (Physical Chemistry) by State University of Campinas - Brazil
1995-2000 - Bachelor of Technological Chemistry by State University of Campinas - Brazil

RESEARCH INTERESTS

Nanotechnology, Drug Delivery System, Photodynamic therapy, Factorial Design, Biochemistry, Genetics and Molecular Biology, Chemistry, Coated Fertilizer

FUTURE PROJECTS

Urea granules coated with Ca and Mg nanoparticles as controlled release pathway for macro and micro nutrients

This project aims to achieve an intelligent release system of granular urea based fertilizer coated with CaCO3 and MgO nanoparticles. The work will seek to: a) determine the influence of the parameters used in the preparation of coated urea granules on properties fundamental to the release kinetics of macro and micronutrients; b) characterize the urea granules in relation to the properties that influence the controlled release of the fertilizer; and c) determine the efficiency of coated and uncoated granulated urea on the cultivation of eucalyptus seedlings.


Applications Invited

Ga (III) -phthalocyanine-containing PLGA-PEG nanospheres: preparation, characterization and evaluation of photodynamic efficiency in MCF-7 tumor cells

This research project has as its general objective the preparation, characterization and evaluation of photodynamic efficiency of polymeric lactide-co-glycolide acid (PLGA) nanospheres linked or not to polyethylene glycol (PLGA-PEG) molecules, containing phthalocyanine of gallium (GaPc) as a photosensitizer in reducing the viability of MCF-7 breast tumor cells. The influence of parameters involved in the preparation of nanospheres on nanoparticulate properties will be studied by chemometric procedures. Experiments will be performed to evaluate the effect of photosensitizer encapsulation on GaPc photobleaching as well as on the side effect of photosensitivity on non-cancerous cells.


Applications Invited
23

Scopus Publications

Scopus Publications

  • Sustainable adsorption of organic dyes from water using brewery spent grain: kinetic, computational and equilibrium studies
    Lenilson Eustaquio de Souza Junior, Allan de Oliveira Gasparoni Pinto, Gabriel Fernandes Souza dos Santos, Araceli Veronica Flores Nardy Ribeiro, Andre Romero da Silva, et al.
    Surfaces and Interfaces, 2026
  • Impact of functionalization linker chemistry on cellulase immobilization: a comparative study of TEOS, APTS, and MPTS functionalized magnetic nanoparticles
    Larissa Lamburghini Brandão, Thais de Andrade Silva, Ana Carolina de Lima Barizão, Larissa Bernardino Moro, Julyana Noval de Souza Ferreira, et al.
    Rsc Advances, 2026
    Magnetic nanobiocatalysts based on Fe 3 O 4 coated with TEOS and functionalised with APTS or MPTS were developed and evaluated for cellulase immobilisation.
  • Magnetite Nanoparticles Encapsulated with PBS-PEG for AMF Hyperthermia
    Carlos Augusto Zanoni Souto, Fernando Gomes de Souza Junior, André Romero da Silva, Kaushik Pal
    Materials, 2025
    Novel studies on typical synthesized magnetite nanoparticles were encapsulated into a poly (butylene succinate)/poly (ethylene glycol) copolymer (PBS-PEG). PBS was chosen because of its biocompatibility characteristics necessary for biomedical applications. PEG, as part of the macromolecular structure, increases the hybrid system’s solubility in an aqueous environment, increasing the circulation time of the material in the bloodstream. The immune system has difficulty recognizing particles with good solubility in an aqueous medium and with a diameter until 200 nm, preventing the body from eliminating the nanoparticles before the magnetic hyperthermia is performed. All the prepared materials were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic light scattering (DLS), and zeta potential. The detailed investigated result executes the formulation developed in this work, showing it has potential and that further studies and analyses can be carried out so that the formulation can be improved, thus obtaining even better results.
  • PLGA nanoparticles for treatment of cardiovascular diseases
    Julyana Noval de Souza Ferreira, Vannyla Viktória Viana Vasconcelos, Barbara Silva Figueiredo, Demian Paiva Alves, Antony Luca Luna Vieira de Abreu, et al.
    Poly Lactic Co Glycolic Acid Plga Nanoparticles for Drug Delivery, 2023
  • Interaction effects between macromolecules and photosensitizer on the ability of AlPc and InPc-loaded PHB magnetic nanoparticles in photooxidatizing simple biomolecules
    Barbara Silva Figueiredo, Julyana Noval de Souza Ferreira, Vannyla Viktória Viana Vasconcelos, Joselito Nardy Ribeiro, Marco Cesar Cunegundes Guimarães, et al.
    International Journal of Biological Macromolecules, 2022
  • Controlled biosynthesis of gold nanoparticles with Coffea arabica using factorial design
    Wanderson Juvencio Keijok, Rayssa Helena Arruda Pereira, Luis Alberto Contreras Alvarez, Adilson Ribeiro Prado, André Romero da Silva, et al.
    Scientific Reports, 2019
    Green synthesis of metallic nanoparticles has become incredibly popular, mainly by minimizing problems of environmental contamination and by being able to reduce, stabilize and potentially functionalize nanomaterials. Such compounds have possible applications in various areas, e.g., pharmaceuticals (drug delivery systems, cosmetics), textile industry (clothing with antimicrobial properties), diagnostic medicine (imaging, high efficiency biosensors), energy (solar panels), bioremediation, among others. However, the lack of reproducibility and information on the control mechanisms during synthesis have made the application of green-synthesized nanoparticles unfeasible. Thus, this study proposed the investigation of the main mechanisms affecting synthesis control, using factorial design for the preparation of gold nanoparticles with extract of Coffea arabica. We obtained stable (Zeta Potential, UV-vis and DLS), monodisperse, and quasi-spherical (TEM) nanoparticles, which presented adsorbed aromatic molecules (FTIR and RAMAN) and defined crystal structure (XRD), proving that the plant extract acted as a reducing agent, as well as a stabilizer and functionalizer for the synthesized nanostructures. The factorial design employed here to obtain gold nanoparticles with Coffea arabica extract allowed for a controlled and reproducible synthesis, enabling new possibilities for the application in several fields.
  • Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin
    Felipe Guzansky Milanezi, Leandra Martins Meireles, Marcella Malavazi de Christo Scherer, Jairo P. de Oliveira, André Romero da Silva, et al.
    Saudi Pharmaceutical Journal, 2019
    In the present work, we report the antioxidant, antimicrobial and cytotoxic activities of quercetin-capped gold nanoparticles (AuNPsQct). The synthesis of AuNPsQct was confirmed by UV–Vis spectroscopy, FTIR and transmission electron microscopy (TEM) analyses. The FTIR spectrum showed the integrity of the quercetin molecules on the nanoparticle surface. The TEM images showed sizes less than 100 nm and a slight spherical shape. The electrostatic stability was confirmed by the zeta potential method. The antioxidant activity of quercetin, evaluated by DPPH, ABTS and nitric oxide free radical scavenging methods, was preserved in the gold nanoparticles, furthermore quercetin-capped gold nanoparticles (IR50 0.37 µg/mL) demonstrated a higher antioxidant activity than free quercetin (IR50 0.57 µg/mL) by nitric oxide free radical scavenging method. Strong antifungal activity was observed for Aspergillus fumigatus with concentrations ranging from 0.1 to 0.5 mg/mL. The nanoparticles with quercetin did not exhibit cytotoxicity to human fibroblasts (L929 cells). In conclusion, these results suggest that AuNPsQct, produced by cost-effective method, can act as a promising candidate for different medical applications.
  • PLGA-PEG nanoparticles containing gallium phthalocyanine: Preparation, optimization and analysis of its photodynamic efficiency on red blood cell and Hepa-1C1C7
    Drielly Lorenzoni, Carlos Augusto Zanoni Souto, Maiara Barboza Araujo, Carol de Souza Berger, Luciene Cristina Duarte da Silva, et al.
    Journal of Photochemistry and Photobiology B Biology, 2019
    Poly(lactide-co-glycolide) (PLGA) has been used for the encapsulation of phthalocyanine motived by its biocompatibility and biodegradability. Many studies have already been done to evaluate the influence of parameters used in the PLGA nanoparticle synthesis but without the evaluation of the combinatory interaction between these parameters on the nanoparticulate properties. Ga(III)-phthalocyanine (GaPc) was encapsulated into the PEGlated PLGA-nanoparticles and the individual and combinatory effects of the emulsification time, the method used for the nanoparticle synthesis and the temperature of the aqueous phase was evaluated on the size, entrapment efficiency, efficacy of nanoparticle recovery, residual PVA and zeta potential value using a 23 factorial design (FD). Mathematical models were adjustable to the data and evolutionary operations were performed to optimize the nanoparticle size. The ability of the optimized nanoparticle to decrease the viability of the Hepa-1C1C7 cell and the blood red cell was also evaluated. The FD disclosed the emulsification-diffusion method decreased the residual PVA and the size of PLGA-PEG nanoparticle, but also decreased the entrapment efficiency of GaPc, the zeta potential absolute value and the recovery efficacy of nanoparticles. The combinatory effect between the method used in the nanoparticle preparation and the temperature of aqueous phase influenced four of the five evaluated properties. The viability of Hepa-1C1C7 cells was reduced until 13× when the cells were irradiated in the presence of encapsulated GaPc while it was decreased until 4.7× when the experiment was carried out with the free GaPc. The encapsulated GaPc was also more efficient to cause the haemolysis of the RBC than it was the free GaPc. The optimization of the nanoparticles synthesis increased the efficiency of the GaPc to oxidize the evaluated cells.
  • Virola oleifera-capped gold nanoparticles showing radical-scavenging activity and low cytotoxicity
    Andressa dos Santos Corrêa, Luis Alberto Contreras, Wanderson Juvencio Keijok, Divan Henrique Fernandes Barcelos, Ana Cláudia Hertel Pereira, et al.
    Materials Science and Engineering C, 2018
    The development of effective nanoparticle therapeutics has been hindered by their surface characteristics, such as hydrophobicity and charge. Therefore, the success of biomedical applications with nanoparticles is governed by the control of these characteristics. In this article, we report an efficient green capping method for gold nanoparticles (AuNPs) by a reduction with sodium citrate and capping with Virola oleifera (Vo), which is a green exudate rich in polyphenols and flavonoids. The Vo-capped AuNPs were characterized by UV, DLS, FTIR, Raman, TEM, DPPH, FRAP and their cytotoxicity was evaluated on the viability of Murine macrophage cell. The AuNPs had an average particle size of 15 nm and were stable over a long time, as indicated by their unchanged SPR and zeta potential values. These nanoparticles were assessed for their antioxidant potential using DPPH and FRAP and demonstrated the highest antioxidant activities and low cytotoxicity. We propose that the Virola oleifera-capped AuNPs have potential biomedical applications.
  • Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
    Laryssa Pinheiro Costa Silva, Jairo Pinto Oliveira, Wanderson Juvencio Keijok, André Romero da Silva, Anderson Rocha Aguiar, et al.
    International Journal of Nanomedicine, 2017
    The biosynthesis of metallic nanoparticles (NPs) using biological systems such as fungi has evolved to become an important area of nanobiotechnology. Herein, we report for the first time the extracellular synthesis of highly stable silver NPs (AgNPs) using the nematophagous fungus Duddingtonia flagrans (AC001). The fungal cell-free filtrate was analyzed by the Bradford method and 3,5-dinitrosalicylic acid assay and used to synthesize the AgNPs in the presence of a 1 mM AgNO3 solution. They have been characterized by UV–Vis spectroscopy, X-ray diffraction, transmission electron microscopy, dynamic light scattering, Zeta potential measurements, Fourier-transform infrared, and Raman spectroscopes. UV–Vis spectroscopy confirmed bioreduction, while X-ray diffractometry established the crystalline nature of the AgNPs. Dynamic light scattering and transmission electron microscopy images showed approximately 11, 38 nm monodisperse and quasispherical AgNPs. Zeta potential analysis was able to show a considerable stability of AgNPs. The N–H stretches in Fourier-transform infrared spectroscopy indicate the presence of protein molecules. The Raman bands suggest that chitinase was involved in the growth and stabilization of AgNPs, through the coating of the particles. Our results show that the NPs we synthesized have good stability, high yield, and monodispersion.
  • Determination of structural and thermodynamic parameters of bovine α-trypsin isoform in aqueous-organic media
    Dayanne Pinho Rosa, Evaldo Vitor Pereira, Antonio Victor Baioco Vasconcelos, Maria Aparecida Cicilini, André Romero da Silva, et al.
    International Journal of Biological Macromolecules, 2017
  • The photobleaching of the free and encapsulated metallic phthalocyanine and its effect on the photooxidation of simple molecules
    Brenda Gomes Fanchiotti, Marcella Piffer Zamprogno Machado, Letícia Camilato de Paula, Mahmut Durmuş, Tebello Nyokong, et al.
    Journal of Photochemistry and Photobiology B Biology, 2016
  • Facile Synthesis of Monodisperse Gold Nanocrystals Using Virola oleifera
    Bárbara A. Milaneze, Jairo P. Oliveira, Ingrid Augusto, Wanderson J. Keijok, Andressa S. Côrrea, et al.
    Nanoscale Research Letters, 2016
  • Effects of preparation conditions of poly(lactide-co-glycolide) nanoparticles loaded with amphiphilic porphyrins and their photoactivities
    Juliana Machado da Silveira, André Romero da Silva, Mathias O. Senge, Renato Atilio Jorge
    Journal of Nanoscience and Nanotechnology, 2014
  • Gamma trypsin: Purification and physicochemical characterization of a novel bovine trypsin isoform
    Caroline Dutra Lacerda, Antônio Ernani. Teixeira, Jamil Silvano de Oliveira, Silvana Fontoura Silva, Antônio Victor Baioco Vasconcelos, et al.
    International Journal of Biological Macromolecules, 2014
  • Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells
    Carlos Augusto Zanoni Souto, Klésia Pirola Madeira, Daniel Rettori, Mariana Ozello Baratti, Letícia Batista Azevedo Rangel, et al.
    Journal of Nanoparticle Research, 2013
  • Effects of preparation conditions on the characteristics of poly(lactide-co-glycolide) nanospheres loaded with chloro(5,10,15,20- tetraphenylporphyrinato)indium(III)
    André Romero da Silva, Ana Maria de Oliveira, Fábio Augusto, Renato Atilio Jorge
    Journal of Nanoscience and Nanotechnology, 2011
  • Drug release from microspheres and nanospheres of poly(lactide-co- glycolide) without sphere separation from the release medium
    Journal of the Brazilian Chemical Society, 2010
  • In vitro photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) loaded-poly(lactide-co-glycolide) nanoparticles in LNCaP prostate tumour cells
    André Romero da Silva, Natalia Mayumi Inada, Daniel Rettori, Mariana Ozello Baratti, Anibal Eugenio Vercesi, et al.
    Journal of Photochemistry and Photobiology B Biology, 2009
  • Photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato) indium(III)
    André R. da Silva, Alessandra C. Pelegrino, Antonio C. Tedesco, Renato A. Jorge
    Journal of the Brazilian Chemical Society, 2008
  • Type II photooxidation mechanism of biomolecules using chloro(5,10,15,20- tetraphenylporphyrinato)indium(III) as a photosensitizer
    André Romero da Silva, Joselito Nardy Ribeiro, Daniel Rettori, Renato Atilio Jorge
    Journal of the Brazilian Chemical Society, 2008
  • Irradiated cationic mesoporphyrin induces larger damage to isolated rat liver mitochondria than the anionic form
    Natalia M. Inada, André R. da Silva, Renato A. Jorge, Jiri Borecký, Anibal E. Vercesi
    Archives of Biochemistry and Biophysics, 2007
  • Evaluation of photodynamic activity of porphyrins for use in photodynamic therapy through tryptophan photo-oxidation
    J. N. Ribeiro, R. A. Jorge, A. R. da Silva, A. V. Flores, L. M. Ronchi, et al.
    Ecletica Quimica, 2007

Publications

Controlled biosynthesis of gold nanoparticles with Coffea arabica using factorial design
Scientific Reports
2019-12 | journal-article
DOI: 10.1038/s41598-019-52496-9Parte de ISSN: 2045-2322

PLGA-PEG nanoparticles containing gallium phthalocyanine: Preparation, optimization and analysis of its photodynamic efficiency on red blood cell and Hepa-1C1C7
Journal of Photochemistry and Photobiology B: Biology
2019-09 | journal-article
DOI: 10.1016/j. de ISSN: 1011-1344

Quantification of Inter-particle Spacing Caused by Thiol Self-Assembled Monolayers Using Transmission Electron Microscopy
Plasmonics
2019-06 | journal-article
DOI: 10.1007/s11468-018-0853-9Parte de ISSN: 1557-1955Parte de ISSN: 1557-1963

Antioxidant, antimicrobial and cytotoxic activities of gold nanoparticles capped with quercetin
Saudi Pharmaceutical Journal
2019 | journal-article
DOI: 10.1016/j. 2-

Virola oleifera-capped gold nanoparticles showing radical-scavenging activity and low cytotoxicity
Materials Science and Engineering: C
2018-10 | journal-article
DOI: 10.1016/j. de ISSN: 0928-4931

EVALUATION OF LIQUORICE GLYCYRRHIZA GLABRA L. ROOT POWDER AS A NEW ADSORBENT IN THE REMOVAL OF TEXTILE DYES IN AQUEOUS MEDIUM.
International Journal of Advanced Research
2018-08-31 | journal-article
DOI: 10.21474/ijar01/7520Parte de ISSN: 2320-5407

WOOD SAWDUST POWDER FROM CORYMBIA CITRIODORA TO CONGO RED TOXIC DYE ADSORPTION
INDIAN JOURNAL OF APPLIED RESEARCH
2018-07 | journal-article
DOI: 10.36106/ijar

Determination of structural and thermodynamic parameters of bovine α-trypsin isoform in aqueous-organic media
International Journal of Biological Macromolecules
2017-08 | journal-article
DOI: 10.1016/j. de ISSN: 0141-8130

Extracellular biosynthesis of silver nanoparticles using the cell-free filtrate of nematophagous fungus Duddingtonia flagrans
International Journal of Nanomedicine
2017-08 | journal-article
DOI: 10.2147/ de ISSN: 1178-2013

Facile Synthesis of Monodisperse Gold Nanocrystals Using Virola oleifera
Nanoscale Research Letters
2016-12 | journal-article
DOI: 10.1186/s11671-016-1683-3Parte de ISSN: 1931-7573Parte de ISSN: 1556-276X

The photobleaching of the free and encapsulated metallic phthalocyanine and its effect on the photooxidation of simple molecules
Journal of Photochemistry and Photobiology B: Biology
2016-12 | journal-article
DOI: 10.1016/j. de ISSN: 1011-1344

Banana Peel for Acetylsalicylic Acid Retention
Journal of Environmental Protection
2016 | journal-article
DOI: 10.4236/ de ISSN: 2152-2197Parte de ISSN: 2152-2219

Gamma trypsin: Purification and physicochemical characterization of a novel bovine trypsin isoform
International Journal of Biological Macromolecules
2014-09 | journal-article
DOI: 10.1016/j. de ISSN: 0141-8130

Effects of Preparation Conditions of Poly(lactide-co-glycolide) Nanoparticles Loaded with Amphiphilic Porphyrins and Their Photoactivities
Journal of Nanoscience and Nanotechnology
2014-08-01 | journal-article
DOI: 10.1166/ de ISSN: 1533-4880Parte de ISSN: 1533-4899

Improved photodynamic action of nanoparticles loaded with indium (III) phthalocyanine on MCF-7 breast cancer cells
Journal of Nanoparticle Research
2013-09 | journal-article
DOI: 10.1007/s11051-013-1879-8Parte de ISSN: 1388-0764Parte de ISSN: 1572-896X

Effects of Preparation Conditions on the Characteristics of Poly(lactide-co-glycolide) Nanospheres Loaded with Chloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
Journal of Nanoscience and Nanotechnology
2011-06-01 | journal-article
DOI: 10.1166/ de ISSN: 1533-4880

Drug release from microspheres and nanospheres of poly(lactide-co- glycolide) without sphere separation from the release medium
Journal of the Brazilian Chemical Society
2010 | journal-article
EID: 2-

Avaliação pré-clínica de novas substâncias para uso como futuras drogas fotoquimioterápicas
ARQUIVOS CATARINENSES DE MEDICINA
2009 | journal-article

In vitro photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) loaded-poly(lactide-co-glycolide) nanoparticles in LNCaP prostate tumour cells
Journal of Photochemistry and Photobiology B: Biology
2009 | journal-article
DOI: 10.1016/j. 2-

Photodynamic activity of chloro(5,10,15,20-tetraphenylporphyrinato) indium(III)
Journal of the Brazilian Chemical Society
2008 | journal-article
EID: 2-

Type II photooxidation mechanism of biomolecules using chloro(5,10,15,20- tetraphenylporphyrinato)indium(III) as a photosensitizer
Journal of the Brazilian Chemical Society
2008 | journal-article
EID: 2-

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

2012/05
BR1020120105772
Pharmaceutical composition comprising nanospheres encapsulated indium phthalocyanine of a polymer and its preparation process with control of nanoparticulate properties

2008/10
PI08043787
Pharmaceutical Composition Comprising Nanospheres Encapsulated Indium Meso-Tetrafenylporphyrin (INTPP) and Process for Preparation thereof

2007/07
PI07030053
Determination process of release profile of encapsulated product

CONSULTANCY

Arbitration of scientific articles at Chemical Paper, Combinatorial Chemistry & High Throughput Screening, Current Nanoscience, Issues in Biological Sciences and Pharmaceutical Research, New Journal of Chemistry, Journal oh Nanoparticle Research, Journal of Nanoscience and Nanotechnology and Tetrahedron,

Industry, Institute, or Organisation Collaboration

Project: Urea granules coated with Ca and Mg nanoparticles as controlled release pathway for macro and micro nutrients
Industry: Granutec Tecnologia de Granulados
Support Source: Granutec Tecnologia de Granulados and Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq
Financing Amount: BRL 76.126,56

INDUSTRY EXPERIENCE

2007/07 to 2008/08 - I worked at EMS Sigma Pharma S.A. as Supervisor Researcher of Project. In this time I work with drug delivery system.