Augmenting phage therapy using green nanotechnology for promising infection control, wound healing and devoiding phage resistance in MDR Pseudomonas aeruginosa Abishek Mani, Aarcha Shanmugha Mary, Nashath Kalangadan, Srivignesh Sundaresan, Somnath Ghosh, John Prakash, Siva Sundara Kumar Durairajan, Kaushik Rajaram Scientific Reports, 2025 Humanity could descend into a pre-antibiotic world as a result of antimicrobial resistance (AMR). Phage defenses are considered "vintage heroes" in the battle against multidrug-resistant (MDR) bacteria. However, phage resistance continues to evolve, necessitating alternative strategies. A potential solution is combining phages with antimicrobials like silver nanoparticles (AgNPs) to enhance the phage therapy. This study focuses on a combination therapy using Bauhinia variegata L.-mediated AgNPs and Pseudomonas phage M12PA to target multidrug-resistant (MDR) Pseudomonas aeruginosa. The antibacterial properties of this combination were evaluated through comprehensive characterizations and assays. As a proof of concept, the AgNP-phage combination delayed phage resistance by 6-12 h, reduced mutation, and enhanced antibacterial efficiency. Anti-biofilm activity improved by ~ 40% compared to AgNPs alone at sub-inhibitory concentrations. AgNPs and phages have demonstrated excellent compatibility, and the phage titer did not drop noticeably over 30 days. Biocompatibility assessments, including cell viability and hemolysis assays, highlighted the safety of biosynthesized AgNPs. Wound healing efficacy was also demonstrated in mouse fibroblast cells and an in ovo angiogenesis model. This study underscores the efficiency of phage-AgNPs synergy to be a potential therapeutic approach for MDR bacterial wound infection, showing effective anti-biofilm activity, delayed phage resistance, and minimal toxicity.
One-Pot Three-Component Cascade Synthesis of Pyrazolopyrido[2,3-h]Quinazolines: Effective Fluorescent Turn-Off Probes for Sensing Pd2+ Ions Philip Raja Stalin, Sepperumal Murugesan, John Prakash, Raju Ranjith Kumar Chemistryselect, 2025 Palladium(II) ions (Pd2+) are crucial in various fields, including catalysis, medicine, electronics, hydrogen storage, sensors, and imaging. However, their uncontrolled use can lead to toxicity and environmental hazards. Therefore, developing selective and sensitive Pd2+ sensing probes is vital for ecological safety, biomedical monitoring, and sustainable resource management. Herein, we report the synthesis of novel pyrazolopyrido[2,3‐h]quinazoline‐based probes (PQMe and PQCl) for the selective and sensitive detection of Pd2+ ions among various metal ions. An environmentally benign multicomponent cascade strategy was employed to synthesize these hybrid heterocycles. The detection limits of the probes PQMe and PQCl for Pd2+ were determined to be 0.55 µM and 0.71 µM, respectively. The binding interaction between the probes and Pd2+ was confirmed through 1H NMR titration experiments. Furthermore, fluorescence lifetime measurements supported the fluorescence quenching mechanism upon Pd2+ binding. The applicability of these probes for real‐world analysis was evaluated by detecting Pd2+ ions in various water samples. Additionally, we demonstrated the potential of these probes to be fabricated into test strips or swabs for efficient on‐site detection of Pd2+ ions.
Relative fitness of wild-type and phage-resistant pyomelanogenic P. aeruginosa and effects of combinatorial therapy on resistant formation Aarcha Shanmugha Mary, Nashath Kalangadan, John Prakash, Srivignesh Sundaresan, Sutharsan Govindarajan, Kaushik Rajaram Heliyon, 2024 Bacteriophages, the natural predators of bacteria, are incredibly potent candidates to counteract antimicrobial resistance (AMR). However, the rapid development of phage-resistant mutants challenges the potential of phage therapy. Understanding the mechanisms of bacterial adaptations to phage predation is crucial for phage-based prognostic applications. Phage cocktails and combinatorial therapy, using optimized dosage patterns of antibiotics, can negate the development of phage-resistant mutations and prolong therapeutic efficacy. In this study, we describe the characterization of a novel bacteriophage and the physiology of phage-resistant mutant developed during infection. M12PA is a P. aeruginosa -infecting bacteriophage with Myoviridae morphology. We observed that prolonged exposure of P. aeruginosa to M12PA resulted in the selection of phage-resistant mutants. Among the resistant mutants, pyomelanin-producing mutants, named PA-M, were developed at a frequency of 1 in 16. Compared to the wild-type, we show that PA-M mutant is severely defective in virulence properties, with altered motility, biofilm formation, growth rate, and antibiotic resistance profile. The PA-M mutant exhibited reduced pathogenesis in an allantoic-infected chick embryo model system compared to the wild-type. Finally, we provide evidence that combinatory therapy, combining M12PA with antibiotics or other phages, significantly delayed the emergence of resistant mutants. In conclusion, our study highlights the potential of combinatory phage therapy to delay the development of phage-resistant mutants and enhance the efficacy of phage-based treatments against P. aeruginosa .
Eco-friendly Gracilaria sp. protein extract–PEGDA hydrogels for fluorescent detection and adsorptive removal of Hg2+ ions C Manikandan, R Krishna, AB Nicy, R Ramakrishnan, J Prakash Algal Research, 104562 , 2026 2026
A label-free approach to simultaneous determination of three endocrine disruptors in river water: angular sweep total fluorescence spectroscopy coupled to multi-way calibration A Vijayan, J Prakash Analyst 151 (2), 529-541 , 2026 2026
Augmenting phage therapy using green nanotechnology for promising infection control, wound healing and devoiding phage resistance in MDR Pseudomonas aeruginosa A Mani, A Shanmugha Mary, N Kalangadan, S Sundaresan, S Ghosh, ... Scientific Reports 15 (1), 30439 , 2025 2025 Citations: 4
Intriguing Role of Cyclodextrin in Fluorescence-Based Metal Ion Sensors M Shahsad, S Jayaram, J Prakash Cyclodextrins for Chemosensing, 105-123 , 2025 2025
The Versatile Roles of Cyclodextrins in Colorimetric and Spectrophotometric Detection of Metal Ions A Vijayan, CK Najiya, J Prakash Cyclodextrins for Chemosensing, 67-85 , 2025 2025
Benzo [4, 5] imidazole [2, 1-b] quinazoline-1 (2H)-one: An efficient fluorescent probe for the selective and sensitive detection of Cu (II) ions SS Roja, PS Sunil, MM Darussalam, V Manoharan, J Prakash, RR Kumar Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 333, 125853 , 2025 2025 Citations: 12
One‐Pot Three‐Component Cascade Synthesis of Pyrazolopyrido[2,3‐ h ]Quinazolines: Effective Fluorescent Turn‐Off Probes for Sensing Pd 2+ Ions PR Stalin, S Murugesan, J Prakash, RR Kumar ChemistrySelect 10 (19), e01643 , 2025 2025 Citations: 2
Investigations into bioavailability and micelle-assisted spectroscopic quantification of brodifacoum in natural samples G Suku, A Vijayan, J Prakash Analytical Methods 17 (20), 4158-4166 , 2025 2025
Emerging analytical methods for quantitative determination of biofuel-petroleum blend composition A Vijayan, J Prakash Analytical Letters 57 (18), 3050-3067 , 2024 2024 Citations: 3
Relative fitness of wild-type and phage-resistant pyomelanogenic P. aeruginosa and effects of combinatorial therapy on resistant formation AS Mary, N Kalangadan, J Prakash, S Sundaresan, S Govindarajan, ... Heliyon 10 (21) , 2024 2024 Citations: 4
Unveiling angular sweep total fluorescence Spectroscopy: A novel multidimensional technique for analysis of complex multi-fluorophoric systems A Vijayan, J Prakash Talanta 271, 125662 , 2024 2024 Citations: 1
Probe-based spectrophotometric quantification of petrol-ethanol fuel blends for field-able applications A Vijayan, J Prakash Green Analytical Chemistry 3, 100043 , 2022 2022 Citations: 4
Fabrication of eco-friendly hydrogel strips for the simultaneous quantification of heavy metal ions in aqueous environment A Mohan, J Prakash Dyes and Pigments 199, 110045 , 2022 2022 Citations: 11
Simultaneous quantification of multiple polycyclic aromatic hydrocarbons in aqueous media using micelle assisted white light excitation fluorescence J Prakash, AK Mishra Scientific Reports 10 (1), 8921 , 2020 2020 Citations: 6
Visualization and quantification of heavy metal ions in drinking water using plant pigments A Mohan, J Prakash Proceedings of the fifteenth DAE-BRNS biennial Trombay symposium on … , 2019 2019
Convenient determination of luminescence quantum yield using a combined electronic absorption and emission spectrometer J Prakash, AK Mishra Review of Scientific Instruments 87 (1) , 2016 2016 Citations: 7
Quantification of doxorubicin in biofluids using white light excitation fluorescence J Prakash, AK Mishra Journal of Biophotonics 7 (8), 607-616 , 2014 2014 Citations: 7
Spectroscopic tool for cancer diagnoses-a preliminary study J Prakash, AK Mishra 2014
Fabrication, optimization and application of a dip-probe fluorescence spectrometer based on white-light excitation fluorescence J Prakash, AK Mishra Measurement Science and Technology 24 (10), 105502 , 2013 2013 Citations: 6
Introduction of a novel fluorescence based method for the composition estimation of petroleum fuel–biofuel blends J Prakash, AK Mishra Fuel 108, 351-355 , 2013 2013 Citations: 15
MOST CITED SCHOLAR PUBLICATIONS
Introduction of a novel fluorescence based method for the composition estimation of petroleum fuel–biofuel blends J Prakash, AK Mishra Fuel 108, 351-355 , 2013 2013.0 Citations: 15
Benzo [4, 5] imidazole [2, 1-b] quinazoline-1 (2H)-one: An efficient fluorescent probe for the selective and sensitive detection of Cu (II) ions SS Roja, PS Sunil, MM Darussalam, V Manoharan, J Prakash, RR Kumar Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 333, 125853 , 2025 2025.0 Citations: 12
White light excitation fluorescence (WLEF) Part II. Analysis of complex multifluorophoric systems J Prakash, AK Mishra Analytical Methods 3 (2), 368-373 , 2011 2011.0 Citations: 12
Fabrication of eco-friendly hydrogel strips for the simultaneous quantification of heavy metal ions in aqueous environment A Mohan, J Prakash Dyes and Pigments 199, 110045 , 2022 2022.0 Citations: 11
White light excitation fluorescence (WLEF) Part I. Exploring the use in analytical fluorimetry J Prakash, AK Mishra Analytical Methods 3 (2), 362-367 , 2011 2011.0 Citations: 10
Convenient determination of luminescence quantum yield using a combined electronic absorption and emission spectrometer J Prakash, AK Mishra Review of Scientific Instruments 87 (1) , 2016 2016.0 Citations: 7
Quantification of doxorubicin in biofluids using white light excitation fluorescence J Prakash, AK Mishra Journal of Biophotonics 7 (8), 607-616 , 2014 2014.0 Citations: 7
Simultaneous quantification of multiple polycyclic aromatic hydrocarbons in aqueous media using micelle assisted white light excitation fluorescence J Prakash, AK Mishra Scientific Reports 10 (1), 8921 , 2020 2020.0 Citations: 6
Fabrication, optimization and application of a dip-probe fluorescence spectrometer based on white-light excitation fluorescence J Prakash, AK Mishra Measurement Science and Technology 24 (10), 105502 , 2013 2013.0 Citations: 6
Augmenting phage therapy using green nanotechnology for promising infection control, wound healing and devoiding phage resistance in MDR Pseudomonas aeruginosa A Mani, A Shanmugha Mary, N Kalangadan, S Sundaresan, S Ghosh, ... Scientific Reports 15 (1), 30439 , 2025 2025.0 Citations: 4
Relative fitness of wild-type and phage-resistant pyomelanogenic P. aeruginosa and effects of combinatorial therapy on resistant formation AS Mary, N Kalangadan, J Prakash, S Sundaresan, S Govindarajan, ... Heliyon 10 (21) , 2024 2024.0 Citations: 4
Probe-based spectrophotometric quantification of petrol-ethanol fuel blends for field-able applications A Vijayan, J Prakash Green Analytical Chemistry 3, 100043 , 2022 2022.0 Citations: 4
Emerging analytical methods for quantitative determination of biofuel-petroleum blend composition A Vijayan, J Prakash Analytical Letters 57 (18), 3050-3067 , 2024 2024.0 Citations: 3
Humic acid enhanced solubilization of bromadiolone in aqueous media J Prakash Advances in Environmental Research 1 (3), 211-221 , 2012 2012.0 Citations: 3
One‐Pot Three‐Component Cascade Synthesis of Pyrazolopyrido[2,3‐ h ]Quinazolines: Effective Fluorescent Turn‐Off Probes for Sensing Pd 2+ Ions PR Stalin, S Murugesan, J Prakash, RR Kumar ChemistrySelect 10 (19), e01643 , 2025 2025.0 Citations: 2
Unveiling angular sweep total fluorescence Spectroscopy: A novel multidimensional technique for analysis of complex multi-fluorophoric systems A Vijayan, J Prakash Talanta 271, 125662 , 2024 2024.0 Citations: 1
Fluorescence Lifetime of Pyrene Butyric Acid as a Versatile Sensing Tool for Monitoring Self-Assembled Systems and Microenvironment J Mishra, A Vijayan, J Swain, J Prakash Citations: 1
Eco-friendly Gracilaria sp. protein extract–PEGDA hydrogels for fluorescent detection and adsorptive removal of Hg2+ ions C Manikandan, R Krishna, AB Nicy, R Ramakrishnan, J Prakash Algal Research, 104562 , 2026 2026.0
A label-free approach to simultaneous determination of three endocrine disruptors in river water: angular sweep total fluorescence spectroscopy coupled to multi-way calibration A Vijayan, J Prakash Analyst 151 (2), 529-541 , 2026 2026.0
Intriguing Role of Cyclodextrin in Fluorescence-Based Metal Ion Sensors M Shahsad, S Jayaram, J Prakash Cyclodextrins for Chemosensing, 105-123 , 2025 2025.0
