MARIA CANDIDA DE GODOY GASPAROTO

@unesp.br

Sao Paulo State University - Campus of Registro - FCAVR - Depart. Agronomy and Natural Resources
Unesp

MARIA CANDIDA DE GODOY GASPAROTO


                           Download Compact PDF  
https://researchid.co/mcggasparoto

RESEARCH, TEACHING, or OTHER INTERESTS

Agronomy and Crop Science, Agricultural and Biological Sciences, Horticulture
22

Scopus Publications

788

Scholar Citations

10

Scholar h-index

11

Scholar i10-index

Scopus Publications

  • Resistance to Triazoles in Populations of Mycosphaerella fijiensis and M. musicola from the Sigatoka Disease Complex from Commercial Banana Plantations in Minas Gerais and São Paulo, Brazil
    Abimael Gomes da Silva, Tatiane Carla Silva, Silvino Intra Moreira, Tamiris Yoshie Kiyama Oliveira, Felix Sebastião Christiano, et al.
    Microorganisms, 2025
    The sterol demethylation inhibitors (DMIs) are among the most widely used fungicides for controlling black Sigatoka (Mycosphaerella fijiensis) and yellow Sigatoka (Mycosphaerella musicola) in banana plantations in Brazil. Black Sigatoka is considered more important due to causing yield losses of up to 100% in commercial banana crops under predisposing conditions. In contrast, yellow Sigatoka is important due to its widespread occurrence in the country. This study aimed to determine the current sensitivity levels of Mf and Mm populations to DMI fungicides belonging to the chemical group of triazoles. Populations of both species were sampled from commercial banana plantations in Registro, Vale do Ribeira, São Paulo (SP), Ilha Solteira, Northwestern SP, and Janaúba, Northern Minas Gerais, and were further characterized phenotypically. Additionally, allelic variation in the CYP51 gene was analyzed in populations of these pathogens to identify and characterize major mutations and/or mechanisms potentially associated with resistance. Sensitivity to the triazoles propiconazole and tebuconazole was determined by calculating the 50% inhibitory concentration of mycelial growth (EC50) based on dose–response curves ranging from 0 to 5 µg mL−1. Variation in sensitivity to fungicides was evident with all nine Mf isolates showing moderate resistance levels to both propiconazole or tebuconazole, while 11 out of 42 Mm strains tested showed low to moderate levels of resistance to these triazoles. Mutations leading to CYP51 substitutions Y136F, Y461N/H, and Y463D in Mm and Y461D, G462D, and Y463D in Mf were associated with low or moderate levels of resistance to the triazoles. Interestingly, Y461H have not been reported before in Mm or Mf populations, and this alteration was found in combination with V106D and A446S. More complex CYP51 variants and CYP51 promoter inserts associated with upregulation of the target protein were not detected and can explain the absence of highly DMI-resistant strains in Brazil. Disease management programs that minimize reliance on fungicide sprays containing triazoles will be needed to slow down the further evolution and spread of novel CYP51 variants in Mf and Mm populations in Brazil.
  • Resistance to Site-Specific Succinate Dehydrogenase Inhibitor Fungicides Is Pervasive in Populations of Black and Yellow Sigatoka Pathogens in Banana Plantations from Southeastern Brazil
    Tatiane C. Silva, Silvino I. Moreira, Daniel M. de Souza, Felix S. Christiano, Maria C. G. Gasparoto, et al.
    Agronomy, 2024
    The Sigatoka disease complex (SDC), caused by Mycosphaerella fijiensis (Mf) and M. musicola (Mm), comprises the most destructive fungal leaf streak and spot diseases of commercial banana crops worldwide. In Brazil, the site-specific succinate dehydrogenase inhibitor (SDHI) fungicides labeled for SDC management since 2014 present a high risk for the emergence of resistance if deployed intensively and solo. Our study determined the levels of sensitivity to boscalid and fluxapyroxad in four populations of the SDC pathogens sampled in 2020 from three distinct geographical regions under contrasting fungicide programs. Resistance, defined as EC50 values exceeding 20 µg mL−1, was prevalent at 59.7% for fluxapyroxad and 94.0% for boscalid. Only 1.5% of isolates exhibited sensitivity to both fungicides. We also assessed the changes in the corresponding fungicide target protein-encoding genes (SdhB, C, and D). None of the target site alterations detected were associated with reduced sensitivity. A second SdhC paralog was also analyzed, but target alterations were not found. However, MDR (multidrug resistance) was detected in a selection of isolates. Further monitoring for Sdh target mutations will be important, but an important role for other resistance mechanisms such as the presence of additional Sdh paralogs and MDR cannot be ruled out. These results highlight the importance of implementing sound anti-resistance management strategies when SDHI fungicides are deployed for the management of SDC.
  • Strategies for managing fungicide resistance in the Brazilian tropical agroecosystem: Safeguarding food safety, health, and the environmental quality
    Paulo Cezar Ceresini, Tatiane Carla Silva, Samara Nunes Campos Vicentini, Rui Pereira Leite Júnior, Silvino Intra Moreira, et al.
    Tropical Plant Pathology, 2024
    Fungicide resistance is an alarming challenge for the Brazilian tropical agricultural systems, with major implications for food safety, human and animal health, as well as for the environment. This review explores strategies to address fungicide resistance within the Brazilian agroecosystem context. We examined historical and current scenarios of fungicide resistance in the Brazilian agroecosystems and the approaches to delay the emergence and mitigate the selection of resistant variants. Our review indicates that the prevalence of resistance in field populations of key plant pathogens in Brazil was due to failures in the implementation of preventive measures. To address this issue, alternative evolutionary-smart strategies against fungicide resistance are proposed, emphasizing institutional actions and public policies. Crucial steps involve strengthening national networks for large-scale foliar and seed fungicide efficacy testing and resistance monitoring, as well as imposing tighter restrictions on the labeling of high-risk single-active formulations. Additionally, the integration of non-chemical disease management strategies and the establishment of a centralized database and information system on fungicide resistance in Brazil are identified as essential for effective resistance monitoring and informed decision-making. To enhance fungicide resistance management, the adoption of a warning system (e.g., based on aerobiology- or on weather-monitoring) for predicting disease epidemics and minimizing fungicide applications is recommended. Increased funding, collaboration, mandatory reporting, and capacity building are required to overcome these challenges. In addition, promoting integrated disease management approaches is vital. By implementing these tailored strategies, Brazil can actively contribute to safeguarding its food safety, protecting human and animal health, and preserving the delicate balance of its unique agroecosystem. The adoption of evolutionary-smart strategies against fungicide resistance will prolong fungicide efficacy, reduce economic costs, and minimize environmental impacts, ensuring sustainable and resilient agriculture in Brazil.
  • Colletotrichum abscissum: Detection on symptomless citrus leaves and symptomatic citrus flowers using high-resolution melting analysis
    Maria Cândida de Godoy de Gasparoto, Andre B. Gama, Nan‐Yi Wang, Carolina S. Rebello, Megan M. Dewdney, et al.
    Plant Pathology, 2023
    High‐resolution melting (HRM) analysis has been recently applied for the diagnosis of plant diseases. This cost‐effective method is advantageous over standard PCR as it does not require DNA band visualization or the use of probes necessary for quantitative PCR. Colletotrichum abscissum causes postbloom fruit drop of citrus (PFD) and survives asymptomatically on vegetative tissue. Quiescent appressoria on citrus leaves are the primary source of inoculum between flowering periods. Early PFD symptoms may be misidentified as physical damage on citrus flowers. Our objectives were to validate an HRM‐based method to rapidly identify C. abscissum on citrus leaves and flowers. We screened seven previously published primer pairs and concluded that the most effective set of primers was CaITS‐F and CaITS‐R815. We evaluated three different DNA extraction methods, two with a purification step (DNeasy Plant Mini and PowerSoil kits) and a faster method without a purification step (buffer GEB2). We inoculated citrus leaves with C. abscissum conidial suspensions of varying concentrations and HRM analysis detected as few as 200 C. abscissum spores using DNeasy Plant Mini or DNeasy PowerSoil kits for DNA extraction. As expected, samples extracted with GEB2 buffer reduced HRM sensitivity. We further collected field samples from areas with high and low C. abscissum inoculum levels. The HRM method could detect the pathogen in high inoculum areas from both leaves and flowers regardless of the extraction method used. In summary, we describe a new molecular diagnostic tool to detect C. abscissum on citrus during its asymptomatic and symptomatic stages.
  • An Accurate, Affordable, and Precise Resazurin-Based Digital Imaging Colorimetric Assay for the Assessment of Fungicide Sensitivity Status of Fungal Populations
    Tatiane Carla Silva, Silvino Intra Moreira, Fabio Gomes Assis, Samara Nunes Campos Vicentini, Abimael Gomes Silva, et al.
    Agronomy, 2023
    This study aimed at the development and validation of an accurate, more affordable, and precise digital imaging resazurin-based fungicide sensitivity colorimetric assay (COL-assay) for fungal plant pathogens from the genera Mycosphaerella and Pyricularia. This proposed digital imaging assay was based on colorimetric estimates of resazurin reduction, which was used as a metabolic indicator of fungal respiration activity on microplate cultures. As fungal model systems, we used the yellow and black Sigatoka pathogens [Mycosphaerella musicola (Mm) and M. fijiensis (Mf), respectively] and the wheat blast pathogen, Pyricularia oryzae Triticum lineage (PoTl), which were previously characterized for QoI, DMI, and SDHI fungicide sensitivity. We then compared the classical spectrophotometry detection assay (SPEC-assay) with the proposed COL-assay based on the analyses of digital images of the microplates’ cultures captured with mobile phone cameras on a handmade trans-illuminator built for poorly equipped labs. Qualitatively, in terms of accuracy, there was full correspondence between the SPEC-assay and the COL-assay according to the fungal EC50 or the relative growth classes on QoI, SDHI, and DMI fungicides for both Mycosphaerella and Pyricularia pathogens. We also observed a strong to very strong correlation coefficient between the COL-assay and the SPEC-assay fungicide sensitivity values for the QoI azoxystrobin, the SDHI fluxapyroxad, and the DMI tebuconazole. Our conclusion was that the COL-assay had a similar accuracy as the SPEC-assay (i.e., resulted in similar fungicide-sensitivity categories for both resistant or sensitive fungal isolates) and high precision. By openly sharing here the COL-assay’s full methodology, and the blueprints of the handmade trans-illuminator, we foresee its adoption by poorly equipped labs throughout the country as an affordable venue for monitoring the fungicide resistance status of populations of important fungal plant pathogens such as M. fijiensis, M. musicola, and P. oryzae Triticum and Oryza lineages.
  • Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis, M. musicola and M. thailandica from Banana Plantations in Southeastern Brazil
    Tamiris Y. K. Oliveira, Tatiane C. Silva, Silvino I. Moreira, Felix S. Christiano, Maria C. G. Gasparoto, et al.
    Agronomy, 2022
    Yellow and black Sigatoka, caused by Mycosphaerella fijiensis and M. musicola, respectively, are the most important worldwide foliar diseases of bananas. Disease control is heavily dependent on intensive fungicide sprays, which increase selection pressure for fungicide resistance in pathogen populations. The primary objective of this study was to assess the level and spread of resistance to quinone-outside inhibitors (QoI—strobilurin) fungicides in populations of both pathogens sampled from banana fields under different fungicide spray regimes in Southeastern Brazil. Secondly, we aimed to investigate when QoI resistance was confirmed if this was associated with the target-site alteration G143A caused by a mutation in the mitochondrial encoded cytochrome b gene. QoI resistance was detected in fungicide treated banana fields, while no resistance was detected in the organic banana field. A total of 18.5% of the isolates sampled from the pathogens’ populations were resistant to QoI. The newly described M. thailandica was also found. It was the second most abundant Mycosphaerella species associated with Sigatoka-like leaf spot symptoms in the Ribeira Valley and the highest level of QoI resistance was found for this pathogen. The G143A cytochrome b alteration was associated with the resistance to the QoI fungicides azoxystrobin and trifloxystrobin in M. fijiensis, M. musicola and M. thailandica strains. In order to reduce resistance development and maintain the efficacy of QoI fungicides, anti-resistance management strategies based on integrated disease management practices should be implemented to control the Sigatoka disease complex.
  • Prevalent Transmission of ‘Candidatus Liberibacter asiaticus’ over ‘Ca. Liberibacter americanus’ in a Long-Term Controlled Environment
    Maria Cândida de Godoy Gasparoto, Isabela Vescove Primiano, Renato B. Bassanezi, Silvia Afonseca Lourenço, Luiz H. Montesino, et al.
    Phytopathology, 2022
    In Brazil, citrus huanglongbing (HLB) is associated with ‘Candidatus Liberibacter americanus’ (CLam) and ‘Ca. Liberibacter asiaticus’ (CLas). However, there are few studies about HLB epidemiology when both Liberibacter spp. and its insect vector, the Asian citrus psyllid (ACP, Diaphorina citri), are present. The objective of this work was to compare the transmission of HLB by ACP when both CLam and CLas are present as primary inoculum. Two experiments were performed under screenhouse conditions from April 2008 to January 2012 (experiment 1) and from February 2011 to December 2015 (experiment 2). The experiments were carried out with sweet orange plants infected with CLam or CLas as inoculum source surrounded by sweet orange healthy plants. One hundred Liberibacter-free adult psyllids were monthly confined to the source of inoculum plants for 7 days with subsequent free movement inside the screenhouse. Fortnightly, nymphs and adults of psyllids were monitored. Psyllid and leaf samples were collected periodically for Liberibacter detection by PCR or quantitative PCR. CLas was detected more frequently than CLam in both psyllid and leaf samples. No mixed infections were detected in the psyllids. A clear prevalence of CLas over CLam was observed in both experiments. The final HLB incidences were 16.7 and 14.5% of Liberibacter-positive test plants, and CLas was detected in 92.3 and 93.1% of these infected plants. Mixed infection was observed only in 3.8% of infected test plants in experiment 1. These results endorse the shift in the prevalence of CLam to CLas observed in citrus orchards of São Paulo, Brazil.
  • Potential reservoirs of a ‘Candidatus Phytoplasma pruni’-related strains (16SrIII-X) associated with HLB-like symptoms in citrus in Brazil
    Júlio C. Barbosa, Maria C. G. Gasparoto, Bárbara Eckstein, Armando Bergamin Filho, Ivan P. Bedendo
    Tropical Plant Pathology, 2021
    Plants of beggarticks (Bidens pilosa L.), honeyweed (Leonurus sibiricus L.), and citrus (Citrus ssp.) trees exhibiting phyllody and virescence, leaf distortion, and yellow shoots symptoms, respectively, were found in orchards at the state of Sao Paulo. Based on PCR assays, computer-simulated RFLP, and phylogenetic analyses, it was confirmed that these plants were infected with a phytoplasma of the subgroup 16SrIII-X, belonging to the ‘Candidatus Phytoplasma pruni’ species. This finding suggested that beggartick and honeyweed plants may be possible reservoirs of 16SrIII-X phytoplasma to citrus tree. The phytoplasma identified in this study presented perfect identity with the phytoplasma previously reported in association with citrus plants harboring HLB-like symptoms, in Brazil.
  • In vitro and in planta studies on temperature adaptation of exserohilum turcicum isolates from maize in europe and south america
    Barbara Ludwig Navarro, Raphael de Araújo Campos, Maria Cândida de Godoy Gasparoto, Andreas von Tiedemann
    Pathogens, 2021
    Northern Corn Leaf Blight (NCLB) is a fungal leaf disease in maize caused by Exserohilum turcicum. NCLB occurs worldwide, from tropical to temperate zones raising the question about plasticity of temperature adaptation of local isolates of the pathogen. Seven isolates of E. turcicum originating from South America and seven from Europe were compared for their response to temperature variations in vitro and in vivo between 15 and 30 °C. In vitro, isolates originating from Europe and South America significantly differed in mycelial growth rate at 30 °C and in sporulation at 25 °C and 30 °C. Aggressiveness of E. turcicum isolates was evaluated on three susceptible maize cultivars (maize lines B37, Sus1 and the German hybrid Niklas) under different day/night temperature regimes (15/10 °C, 20/15 °C, 25/20 °C, or 30/25 °C) with a photoperiod of 14 h. Aggressiveness, recorded as area under the disease progress curve (AUDPC), of South American isolates was higher than for European isolates at 15 °C, 20 °C and 25 °C, and for sporulation in vivo in all temperatures. In general, aggressiveness components were most influenced by temperature. Therefore, multivariate analysis was performed with aggressiveness component data at 30 °C, which expressed the highest number of variables with significant differences between isolate origins. According to their aggressiveness, European and South American isolates can be grouped separately, demonstrating that South American isolates are better adapted to higher temperatures and display a higher level of aggressiveness under similar conditions than European isolates from a cool climate. It is concluded that plasticity of temperature adaptation in E. turcicum populations is relatively large and allowed E. turcicum to follow the recent expansion of maize cultivation into cool climate zones in Europe. However, our data suggest that adaptation to higher temperature is likely to increase aggressiveness of NCLB on maize in cooler climate zones when experiencing further climate warming. This plasticity in adaptation to environmental conditions of E. turcicum may also hamper the success of breeding programs as it may decrease the durability of resistance.
  • Sensitivity of Colletotrichum acutatum Isolates from Citrus to Carbendazim, Difenoconazole, Tebuconazole, and Trifloxystrobin
    Andre B. Gama, Juliana S. Baggio, Carolina S. Rebello, Silvia de Afonseca Lourenço, Maria Cândida de G. Gasparoto, et al.
    Plant Disease, 2020
    Postbloom fruit drop (PFD) of citrus is caused by the Colletotrichum acutatum and C. gloeosporioides species complexes. The disease is important when frequent rainfall occurs during the flowering period of citrus trees. In Brazil, until 2012, PFD was mainly controlled by preventive applications of the methyl-benzimidazole carbamate (MBC) carbendazim and demethylation-inhibitor (DMI) fungicides such as difenoconazole. Since then, mixtures containing the DMI tebuconazole and the quinone-outside inhibitor (QoI) trifloxystrobin have been commonly used. Fungicides are often applied preventively, sometimes even when conditions are not conducive for PFD development. Excessive fungicide applications may favor the selection of resistant populations of Colletotrichum spp. In this study, we assessed the fungicide sensitivity of C. acutatum isolates collected during the two distinct periods of PFD management in Brazil: before and after the trifloxystrobin and tebuconazole mixture became widely employed. The sensitivity of 254 C. acutatum isolates to carbendazim and difenoconazole and of 164 isolates to tebuconazole and trifloxystrobin was assessed. Mycelial growth inhibition of these isolates was evaluated for all the fungicides using either serial dilution of fungicide rates or the spiral gradient dilution method. In addition, inhibition of conidial germination was also assessed for trifloxystrobin. Analysis of partial β-tub, cytb, and cyp51b gene sequences did not reveal any mutations related to resistance to MBCs, QoIs, and DMIs, respectively. In mycelial growth assays, mean EC50 values were 0.14, 0.11, and 0.21 μg/ml for difenoconazole, tebuconazole, and trifloxystrobin, respectively. The conidial germination inhibition by trifloxystrobin was similar among the tested isolates, and the mean EC50 value was 0.002 μg/ml. All isolates had similar mean mycelial growth inhibition for carbendazim, regardless of the fungicide concentrations. Therefore, based on similar EC50 values and molecular analyses, no shift in the sensitivity of isolates has been observed to the fungicides commonly used in different citrus-producing areas in Brazil.
  • Spatiotemporal dynamics of citrus huanglongbing spread: a case study
    M. C. G. Gasparoto, B. Hau, R. B. Bassanezi, J. C. Rodrigues, L. Amorim
    Plant Pathology, 2018
  • A survey on the transport conditions and storage of roses (Rosa spp.) commercialized at CEAGESP flower wholesale in São Paulo City, Brazil
    Mariana Stoppa Pereira, Marcelo Vieira Ferraz, Maria Cândida De Godoy Gasparoto, Thiago De Oliveira
    Ornamental Horticulture, 2018
  • Honeybees can spread Colletotrichum acutatum and C. gloeosporioides among citrus plants
    M. C. G. Gasparoto, S. A. Lourenço, F. A. O. Tanaka, M. B. Spósito, L. C. Marchini, et al.
    Plant Pathology, 2017
  • Influence of temperature on infection and establishment of 'Candidatus Liberibacter americanus' and 'Candidatus Liberibacter asiaticus' in citrus plants
    M. C. G. Gasparoto, H. D. Coletta‐Filho, R. B. Bassanezi, S. A. Lopes, S. A. Lourenço, et al.
    Plant Pathology, 2012
  • Yield loss caused by huanglongbing in different sweet orange cultivars in São Paulo, Brazil
    Renato Beozzo Bassanezi, Luiz Henrique Montesino, Maria Cândida Godoy Gasparoto, Armando Bergamin Filho, Lilian Amorim
    European Journal of Plant Pathology, 2011
  • Detection of mechanical and disease stresses in citrus plants by fluorescence spectroscopy
    J. Belasque, Jr., M. C. G. Gasparoto, L. G. Marcassa
    Applied Optics, 2008
  • Fluorescence spectroscopy applied to orange trees
    L. G. Marcassa, M. C. G. Gasparoto, J. Belasque, E. C. Lins, F. Dias Nunes, et al.
    Laser Physics, 2006
  • Fluorescence spectroscopy for detection of citrus canker in orange plantation
    Optics Infobase Conference Papers, 2006
  • Fluorescence spectroscopy for detection of citrus canker in orange plantation
    Optics Infobase Conference Papers, 2006
  • Fluorescence Spectroscopy for Detection of Citrus Canker in Orange Plantation
    Optics Infobase Conference Papers, 2006
  • Fluorescence spectroscopy for detection of citrus canker in orange plantation
    Optics Infobase Conference Papers, 2006
  • Fluorescence spectroscopy for detection of citrus canker in orange plantation
    E. Lins, J. Belasque Júnior, M. C. G. Gasparoto, V. S. Bagnato, L. G. Marcassa
    Optics Infobase Conference Papers, 2006

RECENT SCHOLAR PUBLICATIONS

  • Aerobiological and molecular monitoring of banana sigatoka pathogens and strobilurin resistance
    AG Silva, LD Krug, TC Silva, SI Moreira, DM Souza, GV Leardine, ...
    Revista Caatinga 38, e12568 , 2025
    2025
  • Resistance to Triazoles in Populations of Mycosphaerella fijiensis and M. musicola from the Sigatoka Disease Complex from Commercial Banana Plantations in …
    AG da Silva, TC Silva, SI Moreira, TYK Oliveira, FS Christiano Jr, ...
    Microorganisms 13 (7), 1439 , 2025
    2025
  • Dispersal of Colletotrichum acutatum Sensu Lato Conidia from Infected Citrus and Strawberry Under Simulated Rainfall and Different Laminar and Turbulent Wind …
    AB Gama, MCG Gasparoto, GH Poole, CH Bock, TR Gottwald, L Amorim, ...
    Phytopathology® 115 (5), 507-520 , 2025
    2025
    Citations: 4
  • Monitoramento aerobiológico e molecular de patógenos das sigatokas da bananeira e da resistência à estrobirulina
    AG Silva, LD Krug, TC Silva, SI Moreira, DM Souza, GV Leardine, ...
    Revista Caatinga 38, e12568 , 2025
    2025
  • Resistance to site-specific succinate dehydrogenase inhibitor fungicides is pervasive in populations of black and yellow Sigatoka pathogens in banana plantations from …
    TC Silva, SI Moreira, DM de Souza, FS Christiano Jr, MCG Gasparoto, ...
    Agronomy 14 (4), 666 , 2024
    2024
    Citations: 8
  • Chemical profile by UPLC-HRMS and antifungal and antioxidant activity of marine macroalgae Dictyota menstrualis
    TC Santos, RCC Martins, MA Alves, J Obando, RC Villaça, DN Cavalcanti, ...
    2024
    Citations: 2
  • An accurate, affordable, and precise resazurin-based digital imaging colorimetric assay for the assessment of fungicide sensitivity status of fungal populations
    TC Silva, SI Moreira, FG Assis Jr, SNC Vicentini, AG Silva, TYK Oliveira, ...
    Agronomy 13 (2), 343 , 2023
    2023
    Citations: 4
  • Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis , M. musicola and M. thailandica from Banana Plantations in …
    TYK Oliveira, TC Silva, SI Moreira, FS Christiano Jr, MCG Gasparoto, ...
    Agronomy 12 (12), 2952 , 2022
    2022
    Citations: 25
  • Prevalent Transmission of ‘Candidatus Liberibacter asiaticus’ over ‘ Ca . Liberibacter americanus’ in a Long-Term Controlled Environment
    MCG Gasparoto, IV Primiano, RB Bassanezi, SA Lourenço, LH Montesino, ...
    Phytopathology® 112 (1), 180-188 , 2022
    2022
    Citations: 18
  • Potential reservoirs of a ‘ Candidatus Phytoplasma pruni’-related strains (16SrIII-X) associated with HLB-like symptoms in citrus in Brazil
    JC Barbosa, MCG Gasparoto, B Eckstein, AB Filho, IP Bedendo
    Tropical Plant Pathology 46 (2), 163-168 , 2021
    2021
    Citations: 2
  • In Vitro and In Planta Studies on Temperature Adaptation of Exserohilum turcicum Isolates from Maize in Europe and South America
    BL Navarro, RA Campos, MCG Gasparoto, A von Tiedemann
    Pathogens 10 (2), 154 , 2021
    2021
    Citations: 14
  • Sensitivity of Colletotrichum acutatum Isolates from Citrus to Carbendazim, Difenoconazole, Tebuconazole, and Trifloxystrobin
    AB Gama, JS Baggio, CS Rebello, SA Lourenco, MCG Gasparoto, ...
    Plant disease 104 (6), 1621-1628 , 2020
    2020
    Citations: 48
  • Colletotrichum acutatum isolates from citrus to carbendazim, difenoconazole, tebuconazole, and trifloxystrobin.
    AB Gama, JS Baggio, CS Rebello, S de Afonseca Lourenço, ...
    2020
  • In vitro control of Colletotrichum acutatum isolates from citrus using extract from brown alga Dictyota dichotoma
    RE da Silva, CS Rebello, LP Machado, GJ Silva, MCDG Gasparoto
    Plant Health 2019 , 2019
    2019
  • Spatiotemporal dynamics of citrus huanglongbing spread: a case study
    MCG Gasparoto, B Hau, RB Bassanezi, JC Rodrigues, L Amorim
    Plant pathology 67 (7), 1621-1628 , 2018
    2018
    Citations: 35
  • A survey on the transport conditions and storage of roses ( Rosa spp.) commercialized at CEAGESP flower wholesale in São Paulo City, Brazil
    MS Pereira, MV Ferraz, MCDG Gasparoto, TD Oliveira
    Ornamental Horticulture 24, 125-137 , 2018
    2018
    Citations: 2
  • Dispersal of Colletotrichum gloeosporioides by citrus pollen
    MC Gasparoto, AB Gama, SA Lourenço, GJ Silva Junior, L Amorim
    Phytopathology 108 (10S supl.), S1. 198-199 , 2018
    2018
  • Honeybees can spread Colletotrichum acutatum and C. gloeosporioides among citrus plants
    MCG Gasparoto, SA Lourenço, FAO Tanaka, MB Spósito, LC Marchini, ...
    Plant Pathology 66 (5), 777-782 , 2017
    2017
    Citations: 30
  • Infectious period of citrtus black spot on sweet orange
    SA Lourenço, MCG Gasparoto, MB Spósito, L Amorim, TR Gottwald
    2013
    Citations: 3
  • Dispersal of Colletotrichum acutatum among citrus plants by honeybees in screenhouse
    MCG Gasparoto, SA Lourenço, PG Assis, LC Escalotin, MB Sposito, ...
    2013

MOST CITED SCHOLAR PUBLICATIONS

  • Detection of mechanical and disease stresses in citrus plants by fluorescence spectroscopy
    J Belasque Jr, MCG Gasparoto, LG Marcassa
    Applied Optics 47 (11), 1922-1926 , 2008
    2008
    Citations: 219
  • Yield loss caused by huanglongbing in different sweet orange cultivars in São Paulo, Brazil
    RB Bassanezi, LH Montesino, MCG Gasparoto, A Bergamin Filho, ...
    European journal of plant pathology 130 (4), 577-586 , 2011
    2011
    Citations: 203
  • Fluorescence spectroscopy applied to orange trees
    LG Marcassa, MCG Gasparoto, J Belasque Jr, EC Lins, FD Nunes, ...
    Laser physics 16 (5), 884-888 , 2006
    2006
    Citations: 67
  • Influence of temperature on infection and establishment of ‘ Candidatus Liberibacter americanus’ and ‘ Candidatus Liberibacter asiaticus’ in citrus plants
    MCG Gasparoto, HD Coletta‐Filho, RB Bassanezi, SA Lopes, ...
    Plant Pathology 61 (4), 658-664 , 2012
    2012
    Citations: 66
  • Sensitivity of Colletotrichum acutatum Isolates from Citrus to Carbendazim, Difenoconazole, Tebuconazole, and Trifloxystrobin
    AB Gama, JS Baggio, CS Rebello, SA Lourenco, MCG Gasparoto, ...
    Plant disease 104 (6), 1621-1628 , 2020
    2020
    Citations: 48
  • Spatiotemporal dynamics of citrus huanglongbing spread: a case study
    MCG Gasparoto, B Hau, RB Bassanezi, JC Rodrigues, L Amorim
    Plant pathology 67 (7), 1621-1628 , 2018
    2018
    Citations: 35
  • Honeybees can spread Colletotrichum acutatum and C. gloeosporioides among citrus plants
    MCG Gasparoto, SA Lourenço, FAO Tanaka, MB Spósito, LC Marchini, ...
    Plant Pathology 66 (5), 777-782 , 2017
    2017
    Citations: 30
  • Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis , M. musicola and M. thailandica from Banana Plantations in …
    TYK Oliveira, TC Silva, SI Moreira, FS Christiano Jr, MCG Gasparoto, ...
    Agronomy 12 (12), 2952 , 2022
    2022
    Citations: 25
  • Prevalent Transmission of ‘Candidatus Liberibacter asiaticus’ over ‘ Ca . Liberibacter americanus’ in a Long-Term Controlled Environment
    MCG Gasparoto, IV Primiano, RB Bassanezi, SA Lourenço, LH Montesino, ...
    Phytopathology® 112 (1), 180-188 , 2022
    2022
    Citations: 18
  • In Vitro and In Planta Studies on Temperature Adaptation of Exserohilum turcicum Isolates from Maize in Europe and South America
    BL Navarro, RA Campos, MCG Gasparoto, A von Tiedemann
    Pathogens 10 (2), 154 , 2021
    2021
    Citations: 14
  • Yield reduction caused by Huanglongbing in different sweet Orange cultivars in São Paulo, Brazil
    RB Bassanezi, LH Montesino, MCG Gasparoto, L Amorim
    International Research Conference on Huanglongbing, Orlando 2008, 237-238 , 2008
    2008
    Citations: 10
  • Resistance to site-specific succinate dehydrogenase inhibitor fungicides is pervasive in populations of black and yellow Sigatoka pathogens in banana plantations from …
    TC Silva, SI Moreira, DM de Souza, FS Christiano Jr, MCG Gasparoto, ...
    Agronomy 14 (4), 666 , 2024
    2024
    Citations: 8
  • First report of ' Candidatus Liberibacter americanus' transmission from Murraya paniculata to sweet orange by Diaphorina citri .
    MCG Gasparoto, RB Bassanezi, L Amorim, LH Montesino, SA Lourenco, ...
    2010
    Citations: 8
  • Relationship between insecticide sprays and huanglongbing progress in a citrus orchard in Sao Paulo, Brazil
    A Berhamin-Filho, MCG Gasparoto, RB Bassanezi, L Amorim
    Proceedings of the International Research Conference on Huanglongbing … , 2008
    2008
    Citations: 8
  • Fluorescence spectroscopy to detect water stress in orange trees
    EC Lins, FD Nunes, MCG Gasparoto, JB Junior, VS Bagnato, ...
    SBMO/IEEE MTT-S International Conference on Microwave and Optoelectronics … , 2005
    2005
    Citations: 6
  • Dispersal of Colletotrichum acutatum Sensu Lato Conidia from Infected Citrus and Strawberry Under Simulated Rainfall and Different Laminar and Turbulent Wind …
    AB Gama, MCG Gasparoto, GH Poole, CH Bock, TR Gottwald, L Amorim, ...
    Phytopathology® 115 (5), 507-520 , 2025
    2025
    Citations: 4
  • An accurate, affordable, and precise resazurin-based digital imaging colorimetric assay for the assessment of fungicide sensitivity status of fungal populations
    TC Silva, SI Moreira, FG Assis Jr, SNC Vicentini, AG Silva, TYK Oliveira, ...
    Agronomy 13 (2), 343 , 2023
    2023
    Citations: 4
  • Infectious period of citrtus black spot on sweet orange
    SA Lourenço, MCG Gasparoto, MB Spósito, L Amorim, TR Gottwald
    2013
    Citations: 3
  • Number of insecticide sprays has no effect on the incidente of citrus huanglongbing ina commercial orchad in São Paulo, Brazil
    A Bergamin Filho, MG Gasparoto, L Amorim, RB Bassanezi
    Phtopathology 98 (6), S21 , 2008
    2008
    Citations: 3
  • Chemical profile by UPLC-HRMS and antifungal and antioxidant activity of marine macroalgae Dictyota menstrualis
    TC Santos, RCC Martins, MA Alves, J Obando, RC Villaça, DN Cavalcanti, ...
    2024
    Citations: 2