DR ANKUR SRIVASTAVA

@iitrindia.org

Research Associate in Systems Toxicology Group
CSIR-INDIAN INSTITUTE OF TOXICOLOGY RESEARCH

DR ANKUR SRIVASTAVA


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https://researchid.co/ank.biotech

EDUCATION

M.Sc. in Biotechnology
Ph.D. in Biotechnology, Biochemistry, Neurobiology

RESEARCH, TEACHING, or OTHER INTERESTS

Neuroscience, General Neuroscience, Molecular Biology, Cell Biology
20

Scopus Publications

176

Scholar Citations

8

Scholar h-index

5

Scholar i10-index

Scopus Publications

  • Mesenchymal stem cell secretome restores monocrotophos induced toxicity in human neural progenitor cells
    P. Vatsa, A. Srivastava, A.K. Srivastava, A. Pandeya, A. Singh, A.B. Pant
    Biochemical and Biophysical Research Communications, 2025
  • Targeting TDP-43 Proteinopathy in hiPSC-Derived Mutated hNPCs with Mitoxantrone Drugs and miRNAs
    Uzair A. Ansari, Ankita Srivastava, Ankur K. Srivastava, Abhishek Pandeya, Pankhi Vatsa, Renu Negi, Akash Singh, Aditya B. Pant
    Pharmaceutics, 2025
    Background/Objectives: TDP-43 mutation-driven Amyotrophic Lateral Sclerosis (ALS) motor neuron disease is one of the most prominent forms (approximately 97%) in cases of sporadic ALS. Dysfunctional autophagy and lysosomal function are the prime mechanisms behind ALS. Mitoxantrone (Mito), a synthetic doxorubicin analog, is an inhibitor of DNA and RNA synthesis/repair via intercalating with nitrogenous bases and inhibiting topoisomerase II. The therapeutic potential of miRNAs associated with disease conditions has also been reported. This study explores the therapeutic potential of Mito along with miRNAs against mutated TDP-43 protein-induced proteinopathy in human-induced pluripotent stem cell (hiPSC)-derived human neural progenitor cells (hNPCs). Methods: HiPSCs mutated for TDP-43 were differentiated into hNPCs and used to explore the therapeutic potential of Mito at a concentration of 1 μM for 24 h (the identified non-cytotoxic dose). The therapeutic effects of Mito on miRNA expression and various cellular parameters such as mitochondrial dynamics, autophagy, and stress granules were assessed using the high-throughput Open Array technique, immunocytochemistry, flow cytometry, immunoblotting, and mitochondrial bioenergetic assay. Results: Mutated TDP-43 protein accumulation causes stress granule formation (G3BP1), mitochondrial bioenergetic dysfunction, SOD1 accumulation, hyperactivated autophagy, and ER stress in hNPCs. The mutated hNPCs also show dysregulation in six miRNAs (miR-543, miR-34a, miR-200c, miR-22, miR-29b, and miR-29c) in mutated hNPCs. A significant restoration of TDP-43 mutation-induced alterations could be witnessed upon the exposure of mutated hNPCs to Mito. Conclusions: Our study indicates that miR-543, miR-29b, miR-22, miR-200c, and miR-34a have antisense therapeutic potential alone and in combination with Mitoxantrone.
  • Proteomic-miRNA Biomics Profile Reveals 2D Cultures of Human iPSC-Derived Neural Progenitor Cells More Sensitive than 3D Spheroid System Against the Experimental Exposure to Arsenic
    R. Negi, A. Srivastava, A. K. Srivastava, P. Vatsa, U. A. Ansari, B. Khan, H. Singh, A. Pandeya, AB Pant
    Molecular Neurobiology, 2024
  • Strategic Developments for Pre-clinical Safety/Efficacy Studies of Hair Care Products
    Ankita Srivastava, Ankur Kumar Srivastava, A. B. Pant
    Hair Care Products Efficacy Safety and Global Regulation, 2024
  • A protein–miRNA biomic analysis approach to explore neuroprotective potential of nobiletin in human neural progenitor cells (hNPCs)
    Sadaf Jahan, Uzair Ahmad Ansari, Ankur Kumar Srivastava, Sahar Aldosari, Nessrin Ghazi Alabdallat, Arif Jamal Siddiqui, Andleeb Khan, Hind Muteb Albadrani, Sana Sarkar, Bushra Khan, Mohd Adnan, Aditya Bhushan Pant
    Frontiers in Pharmacology, 2024
    Chemical-induced neurotoxicity is increasingly recognized to accelerate the development of neurodegenerative disorders (NDs), which pose an increasing health burden to society. Attempts are being made to develop drugs that can cross the blood–brain barrier and have minimal or no side effects. Nobiletin (NOB), a polymethoxylated flavonoid with anti-oxidative and anti-inflammatory effects, has been demonstrated to be a promising compound to treat a variety of NDs. Here, we investigated the potential role of NOB in sodium arsenate (NA)-induced deregulated miRNAs and target proteins in human neural progenitor cells (hNPCs). The proteomics and microRNA (miRNA) profiling was done for different groups, namely, unexposed control, NA-exposed, NA + NOB, and NOB groups. Following the correlation analysis between deregulated miRNAs and target proteins, RT-PCR analysis was used to validate the selected genes. The proteomic analysis showed that significantly deregulated proteins were associated with neurodegeneration pathways, response to oxidative stress, RNA processing, DNA repair, and apoptotic process following exposure to NA. The OpenArray analysis confirmed that NA exposure significantly altered miRNAs that regulate P53 signaling, Wnt signaling, cell death, and cell cycle pathways. The RT-PCR validation studies concur with proteomic data as marker genes associated with autophagy and apoptosis (HO-1, SQSTM1, LC-3, Cas3, Apaf1, HSP70, and SNCA1) were altered following NA exposure. It was observed that the treatment of NOB significantly restored the deregulated miRNAs and proteins to their basal levels. Hence, it may be considered one of its neuroprotective mechanisms. Together, the findings are promising to demonstrate the potential applicability of NOB as a neuroprotectant against chemical-induced neurotoxicity.
  • Pesticide mediated silent neurotoxicity and its unmasking: An update on recent progress
    Ankita Srivastava, Ankur Kumar Srivastava, Abhishek Pandeya, Aditya Bhushan Pant
    Toxicology, 2023
  • Proteome architecture of human‑induced pluripotent stem cell‑derived three‑dimensional organoids as a tool for early diagnosis of neuronal disorders
    R. Negi, A. Srivastava, A. Srivastava, Abhishek Pandeya, P. Vatsa, U. A. Ansari, A. Pant
    Indian Journal of Pharmacology, 2023
    BACKGROUND AND OBJECTIVES: Induced pluripotent stem cells (iPSCs) derived three-dimensional (3D) model for rare neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS) is emerging as a novel alternative to human diseased tissue to explore the disease etiology and potential drug discovery. In the interest of the same, we have generated a TDP-43-mutated human iPSCs (hiPSCs) derived 3D organoid model of ALS disease. The high-resolution mass spectrometry (MS)-based proteomic approach is used to explore the differential mechanism under disease conditions and the suitability of a 3D model to study the disease. MATERIALS AND METHODS: The hiPSCs cell line was procured from a commercial source, grown, and characterized following standard protocols. The mutation in hiPSCs was accomplished using CRISPR/Cas-9 technology and predesigned gRNA. The two groups of organoids were produced by normal and mutated hiPSCs and subjected to the whole proteomic profiling by high-resolution MS in two biological replicates with three technical replicas of each. RESULTS: The proteomic analysis of normal and mutated organoids revealed the proteins associated with pathways of neurodegenerative disorders, proteasomes, autophagy, and hypoxia-inducible factor-1 signaling. Differential proteomic analysis revealed that the mutation in TDP-43 gene caused proteomic deregulation, which impaired protein quality mechanisms. Furthermore, this impairment may contribute to the generation of stress conditions that may ultimately lead to the development of ALS pathology. CONCLUSION: The developed 3D model represents the majority of candidate proteins and associated biological mechanisms altered in ALS disease. The study also offers novel protein targets that may uncloud the precise disease pathological mechanism and be considered for future diagnostic and therapeutic purposes for various neurodegenerative disorders.
  • A combined microRNA and proteome profiling to investigate the effect of ZnO nanoparticles on neuronal cells
    Ankur Kumar Srivastava, Smriti Singh Yadav, Saumya Mishra, Sanjeev Kumar Yadav, Devendra Parmar, Sanjay Yadav
    Nanotoxicology, 2020
    Zinc oxide nanoparticles (ZnO NPs) are one of the most broadly used engineered nanomaterials. The toxicity potential of ZnO NPs has been explored in several studies; however, its neurotoxicity, especially its molecular mechanism, has not been studied in depth. In this study, we have used a cellular model of neuronal differentiation (nerve growth factor differentiated PC12 cells) to compare the effect of ZnO NPs exposure on neuronal (differentiated or mature neurons) and non-neuronal (undifferentiated) cells. Our studies have shown that the noncytotoxic concentration of ZnO NPs causes neurite shortening and degeneration in differentiated PC12 cells. Brain-specific microRNA (miRNA) array and liquid chromatography with tandem mass spectrometry (LC-MS/MS) are used to carry out profiling of miRNAs and proteins in PC12 cells exposed with ZnO NPs. Exposure of ZnO NPs produced significant deregulation of a higher number of miRNAs (15) and proteins (267) in neuronal cells in comparison to miRNAs (8) and proteins (207) of non-neuronal cells (8). In silico pathway analysis of miRNAs and proteins deregulated in ZnO NPs exposed differentiated PC12 cells have shown pathways leading to neurodegenerative diseases and mitochondrial dysfunctions are primarily targeted pathways. Further, a bioenergetics study carried out using Seahorse XFp metabolic flux analyzer has confirmed the involvement of mitochondrial dysfunctions in ZnO NPs exposed differentiated PC12 cells. In conclusion, differentiated PC12 cells (neuronal) were found more vulnerable than undifferentiated (non-neuronal PC12 cells) toward the exposure of ZnO NPs and deregulation of miRNAs and mitochondrial dysfunctions play a significant role in its toxicity.
  • A proteomic approach to investigate enhanced responsiveness in rechallenged adult rats prenatally exposed to lindane
    Ankita Srivastava, Ankur Kumar Srivastava, Manisha Mishra, Jai Shankar, Anita Agrahari, Mohan Kamthan, Pradhyumna K. Singh, Sanjay Yadav, Devendra Parmar
    Neurotoxicology, 2019
  • Impact of Surface-Engineered ZnO Nanoparticles on Protein Corona Configuration and Their Interactions With Biological System
    Anurag Kumar Srivastav, Nitesh Dhiman, Hafizurrahman Khan, Ankur Kumar Srivastav, Sanjeev Kumar Yadav, Jyoti Prakash, Nidhi Arjaria, Dhirendra Singh, Sanjay Yadav, Satyakam Patnaik, Mahadeo Kumar
    Journal of Pharmaceutical Sciences, 2019
  • Molecular Diagnostics in Melanoma: An Update
    A. Srivastava, P. Srivastava, A. B. Pant
    Molecular Diagnostics in Cancer Patients, 2019
  • Secretome of Differentiated PC12 Cells Enhances Neuronal Differentiation in Human Mesenchymal Stem Cells Via NGF-Like Mechanism
    A. Srivastava, S. Singh, A. Pandey, D. Kumar, C. S. Rajpurohit, V. K. Khanna, A. B. Pant
    Molecular Neurobiology, 2018
  • Resveratrol Prevents the Cellular Damages Induced by Monocrotophos via PI3K Signaling Pathway in Human Cord Blood Mesenchymal Stem Cells
    S. Jahan, D. Kumar, S. Singh, V. Kumar, A. Srivastava, A. Pandey, C. S. Rajpurohit, V. K. Khanna, A. B. Pant
    Molecular Neurobiology, 2018
  • Phytomedicine: A Potential Alternative Medicine in Controlling Neurological Disorders
    A. Srivastava, P. Srivastava, A. Pandey, V.K. Khanna, A.B. Pant
    New Look to Phytomedicine Advancements in Herbal Products as Novel Drug Leads, 2018
  • Secretome of Differentiated PC12 Cells Restores the Monocrotophos-Induced Damages in Human Mesenchymal Stem Cells and SHSY-5Y Cells: Role of Autophagy and Mitochondrial Dynamics
    A. Srivastava, S. Singh, C. S. Rajpurohit, P. Srivastava, A. Pandey, D. Kumar, V. K. Khanna, A. B. Pant
    Neuromolecular Medicine, 2018
  • PKA-GSK3β and β-Catenin Signaling Play a Critical Role in Trans-Resveratrol Mediated Neuronal Differentiation in Human Cord Blood Stem Cells
    S Jahan, S Singh, A Srivastava, V Kumar, D Kumar, A Pandey, CS Rajpurohit, AR Purohit, VK Khanna, AB Pant
    Molecular Neurobiology, 2018
  • Differentiation Induces Dramatic Changes in miRNA Profile, Where Loss of Dicer Diverts Differentiating SH-SY5Y Cells Toward Senescence
    Abhishek Jauhari, Tanisha Singh, Ankita Pandey, Parul Singh, Nishant Singh, Ankur Kumar Srivastava, Aditya Bhushan Pant, Devendra Parmar, Sanjay Yadav
    Molecular Neurobiology, 2017
  • Adoptive Autophagy Activation: a Much-Needed Remedy Against Chemical Induced Neurotoxicity/Developmental Neurotoxicity
    A. Srivastava, V. Kumar, A. Pandey, S. Jahan, D. Kumar, C. S. Rajpurohit, S. Singh, V. K. Khanna, A. B. Pant
    Molecular Neurobiology, 2017
  • Stem Cells in Neurotoxicology/Developmental Neurotoxicology: Current Scenario and Future Prospects
    S. Singh, A. Srivastava, V. Kumar, A. Pandey, D. Kumar, C. S. Rajpurohit, V. K. Khanna, S. Yadav, A. B. Pant
    Molecular Neurobiology, 2016
  • Transactivation of P53 by cypermethrin induced miR-200 and apoptosis in neuronal cells
    Ankita Pandey, Abhishek Jauhari, Tanisha Singh, Parul Singh, Nishant Singh, Ankur Kumar Srivastava, Farah Khan, Aditya Bhushan Pant, Devendra Parmar, Sanjay Yadav
    Toxicology Research, 2015

RECENT SCHOLAR PUBLICATIONS

  • Mesenchymal Stem Cell Secretome Restores Monocrotophos Induced Toxicity in Human Neural Progenitor Cells
    P Vatsa, A Srivastava, AK Srivastava, A Pandeya, A Singh, AB Pant
    Biochemical and Biophysical Research Communications, 151987 , 2025
    2025
  • Targeting TDP-43 Proteinopathy in hiPSC-Derived Mutated hNPCs with Mitoxantrone Drugs and miRNAs
    UA Ansari, A Srivastava, AK Srivastava, A Pandeya, P Vatsa, R Negi, ...
    Pharmaceutics 17 (4), 410 , 2025
    2025
  • Strategic Developments for Pre-clinical Safety/Efficacy Studies of Hair Care Products
    A Srivastava, AK Srivastava, AB Pant
    Hair Care Products: Efficacy, Safety and Global Regulation, 223-273 , 2024
    2024
    Citations: 2
  • A protein–miRNA biomic analysis approach to explore neuroprotective potential of nobiletin in human neural progenitor cells (hNPCs)
    S Jahan, UA Ansari, AK Srivastava, S Aldosari, NG Alabdallat, AJ Siddiqui, ...
    Frontiers in Pharmacology 15, 1343569 , 2024
    2024
    Citations: 8
  • Proteomic-miRNA Biomics Profile Reveals 2D Cultures of Human iPSC-Derived Neural Progenitor Cells More Sensitive than 3D Spheroid System Against the Experimental Exposure to …
    R Negi, A Srivastava, AK Srivastava, P Vatsa, UA Ansari, B Khan, H Singh, ...
    Molecular Neurobiology, 1-17 , 2024
    2024
    Citations: 3
  • Pesticide Mediated Silent Neurotoxicity and Its Unmasking: An Update on Recent Progress
    A Srivastava, AK Srivastava, A Pandeya, AB Pant
    Toxicology, 153665 , 2023
    2023
    Citations: 8
  • Proteome architecture of human-induced pluripotent stem cell-derived three-dimensional organoids as a tool for early diagnosis of neuronal disorders
    R Negi, A Srivastava, AK Srivastava, A Pandeya, P Vatsa, UA Ansari, ...
    Indian Journal of Pharmacology 55 (2), 108 , 2023
    2023
    Citations: 10
  • Proteome profiling of phosphatidylinositol-5-phosphate 4-kinase type 2A and 2B knockdown cells identify modifications in key regulators involved in cell homeostasis and genome …
    P Awasthi, AK Srivastava, VK Yadav, R Singh, SS Yadav, GR Kidiyoor, ...
    Genome Instability & Disease 3 (2), 88-107 , 2022
    2022
    Citations: 1
  • A combined microRNA and proteome profiling to investigate the effect of ZnO nanoparticles on neuronal cells
    AK Srivastava, SS Yadav, S Mishra, SK Yadav, D Parmar, S Yadav
    Nanotoxicology 14 (6), 757-773 , 2020
    2020
    Citations: 31
  • A proteomic approach to investigate enhanced responsiveness in rechallenged adult rats prenatally exposed to lindane
    A Srivastava, AK Srivastava, M Mishra, J Shankar, A Agrahari, M Kamthan, ...
    Neurotoxicology 74, 184-195 , 2019
    2019
    Citations: 6
  • Impact of surface-engineered ZnO nanoparticles on protein corona configuration and their interactions with biological system
    AK Srivastav, N Dhiman, H Khan, AK Srivastav, SK Yadav, J Prakash, ...
    Journal of Pharmaceutical Sciences 108 (5), 1872-1889 , 2019
    2019
    Citations: 30
  • Differentiation induces dramatic changes in miRNA profile, where loss of dicer diverts differentiating SH-SY5Y cells toward senescence
    A Jauhari, T Singh, A Pandey, P Singh, N Singh, AK Srivastava, AB Pant, ...
    Molecular neurobiology 54, 4986-4995 , 2017
    2017
    Citations: 40
  • Transactivation of P53 by cypermethrin induced miR-200 and apoptosis in neuronal cells
    A Pandey, A Jauhari, T Singh, P Singh, N Singh, AK Srivastava, F Khan, ...
    Toxicology Research 4 (6), 1578-1586 , 2015
    2015
    Citations: 29
  • MicroRNAs are emerging as most potential molecular biomarkers
    S Yadav, A Jauhari, N Singh, T Singh, AK Srivastav, P Singh, A Pant, ...
    Biochemistry & Analytical Biochemistry 4 (2161–1009), 1000191 , 2015
    2015
    Citations: 8

MOST CITED SCHOLAR PUBLICATIONS

  • Differentiation induces dramatic changes in miRNA profile, where loss of dicer diverts differentiating SH-SY5Y cells toward senescence
    A Jauhari, T Singh, A Pandey, P Singh, N Singh, AK Srivastava, AB Pant, ...
    Molecular neurobiology 54, 4986-4995 , 2017
    2017
    Citations: 40
  • A combined microRNA and proteome profiling to investigate the effect of ZnO nanoparticles on neuronal cells
    AK Srivastava, SS Yadav, S Mishra, SK Yadav, D Parmar, S Yadav
    Nanotoxicology 14 (6), 757-773 , 2020
    2020
    Citations: 31
  • Impact of surface-engineered ZnO nanoparticles on protein corona configuration and their interactions with biological system
    AK Srivastav, N Dhiman, H Khan, AK Srivastav, SK Yadav, J Prakash, ...
    Journal of Pharmaceutical Sciences 108 (5), 1872-1889 , 2019
    2019
    Citations: 30
  • Transactivation of P53 by cypermethrin induced miR-200 and apoptosis in neuronal cells
    A Pandey, A Jauhari, T Singh, P Singh, N Singh, AK Srivastava, F Khan, ...
    Toxicology Research 4 (6), 1578-1586 , 2015
    2015
    Citations: 29
  • Proteome architecture of human-induced pluripotent stem cell-derived three-dimensional organoids as a tool for early diagnosis of neuronal disorders
    R Negi, A Srivastava, AK Srivastava, A Pandeya, P Vatsa, UA Ansari, ...
    Indian Journal of Pharmacology 55 (2), 108 , 2023
    2023
    Citations: 10
  • A protein–miRNA biomic analysis approach to explore neuroprotective potential of nobiletin in human neural progenitor cells (hNPCs)
    S Jahan, UA Ansari, AK Srivastava, S Aldosari, NG Alabdallat, AJ Siddiqui, ...
    Frontiers in Pharmacology 15, 1343569 , 2024
    2024
    Citations: 8
  • Pesticide Mediated Silent Neurotoxicity and Its Unmasking: An Update on Recent Progress
    A Srivastava, AK Srivastava, A Pandeya, AB Pant
    Toxicology, 153665 , 2023
    2023
    Citations: 8
  • MicroRNAs are emerging as most potential molecular biomarkers
    S Yadav, A Jauhari, N Singh, T Singh, AK Srivastav, P Singh, A Pant, ...
    Biochemistry & Analytical Biochemistry 4 (2161–1009), 1000191 , 2015
    2015
    Citations: 8
  • A proteomic approach to investigate enhanced responsiveness in rechallenged adult rats prenatally exposed to lindane
    A Srivastava, AK Srivastava, M Mishra, J Shankar, A Agrahari, M Kamthan, ...
    Neurotoxicology 74, 184-195 , 2019
    2019
    Citations: 6
  • Proteomic-miRNA Biomics Profile Reveals 2D Cultures of Human iPSC-Derived Neural Progenitor Cells More Sensitive than 3D Spheroid System Against the Experimental Exposure to …
    R Negi, A Srivastava, AK Srivastava, P Vatsa, UA Ansari, B Khan, H Singh, ...
    Molecular Neurobiology, 1-17 , 2024
    2024
    Citations: 3
  • Strategic Developments for Pre-clinical Safety/Efficacy Studies of Hair Care Products
    A Srivastava, AK Srivastava, AB Pant
    Hair Care Products: Efficacy, Safety and Global Regulation, 223-273 , 2024
    2024
    Citations: 2
  • Proteome profiling of phosphatidylinositol-5-phosphate 4-kinase type 2A and 2B knockdown cells identify modifications in key regulators involved in cell homeostasis and genome …
    P Awasthi, AK Srivastava, VK Yadav, R Singh, SS Yadav, GR Kidiyoor, ...
    Genome Instability & Disease 3 (2), 88-107 , 2022
    2022
    Citations: 1
  • Mesenchymal Stem Cell Secretome Restores Monocrotophos Induced Toxicity in Human Neural Progenitor Cells
    P Vatsa, A Srivastava, AK Srivastava, A Pandeya, A Singh, AB Pant
    Biochemical and Biophysical Research Communications, 151987 , 2025
    2025
  • Targeting TDP-43 Proteinopathy in hiPSC-Derived Mutated hNPCs with Mitoxantrone Drugs and miRNAs
    UA Ansari, A Srivastava, AK Srivastava, A Pandeya, P Vatsa, R Negi, ...
    Pharmaceutics 17 (4), 410 , 2025
    2025

Industry, Institute, or Organisation Collaboration

CSIR- Indian Institute of Toxicology Research, Lucknow
National Dope Testing Laboratory, New Delhi