Pharmaceutical Science, Chemistry, Drug Discovery, General Pharmacology, Toxicology and Pharmaceutics
21
Scopus Publications
Scopus Publications
IN VITRO, EX-VIVO, AND DISSOLUTION KINETIC STUDIES OF MOUTH-DISSOLVING SUBLINGUAL FILMS CONTAINING LOSARTAN POTASSIUM AND HYDROCHLOROTHIAZIDE VINAYATA ATTAL, KRATIKA DANIEL Asian Journal of Pharmaceutical and Clinical Research, 2025 Objectives: Sublingual films were designed for easy administration, rapid as well as slow drug absorption for obtaining controlled release action through the oral mucosa, bypassing the gastrointestinal tract and hepatic first-pass metabolism. This can result in a faster onset of action, enhanced bioavailability, and patient convenience since water isn’t required for administration. Films also offer precise dosing, enhanced drug stability, and targeted therapy for localized treatment within the oral cavity. These features make a non-invasive and painless alternative to injections, improving the patient experience. This paper focuses on in vitro and ex vivo activity using goat oral mucosa and the suitability of release kinetics using different kinetic parameters. Methods: Films were generated using a solvent casting method where a polymer, drug, plasticizers, and excipients were dissolved in a solvent to form a homogenous mixture. This mixture was poured into a petri dish, forming a thin layer. After controlled evaporation, the resulting film was cut to the desired size and shape and stored away from light and heat. Results: Ex vivo studies using goat buccal mucosa showed effective drug absorption. F4 batch was considered an optimized batch because it showed a release of 79.23±1.15% in 9 h in in vitro release study and ex vivo studies showed the highest permeation as compared to other batches and is found to be 96.54±1.23% in 9 h and the flux was calculated to be 0.975±1.15 mg h−1cm−2, 0.904±0.92 mg h−1cm−2, and 0.901 mg h−1cm−2 (Target flux 0.991 mg h−1cm−2).Two-way ANOVA suggested p<0.05. The drug release was best described by the Higuchi square root model, indicating a diffusion-controlled process. Conclusions: The film containing losartan potassium and hydrochlorothiazide offers a significant advancement in hypertension treatment. This approach boosts therapeutic outcomes and patient quality of life by overcoming demands associated with traditional oral dosage forms. Further research could extend the benefits of film to other medications and conditions, enhancing the field of drug delivery.
Nephroprotective, Hepatoprotective and Toxicological Studies of Vigna mungo Aqueous Extract , Kratika Daniel, Shivanee G Phalphale, and International Journal of Drug Delivery Technology, 2025 Hepatitis and nephropathy are significant global public health issues. The liver plays a crucial role in the body's glucose,protein, and lipid metabolism while also facilitating the elimination of waste products and harmful metabolites, therebypreventing toxicity. Similarly, the kidneys, being key components of the excretory system, are particularly vulnerable to awide array of toxic agents, chemicals, and drug-related injuries. About 80% of people worldwide use traditional medicineto treat a variety of illnesses, according to the World Health Organisation (WHO). However, scientific studies reveal thatonly about 1% of these plants exhibit therapeutic potential when consumed in extract form by humans. Given the absenceof stable liver and kidney protective drugs in conventional allopathic medicine, herbal remedies play a vital role inmanaging liver and kidney disorders. Herbal medicines are often considered safe and free from significant side effects dueto their natural origin and widespread availability. The seeds of Vigna mungo are traditionally utilised in treatments forconditions such as paralysis, rheumatism, nervous system disorders, fever, and as a diuretic and tonic. These seeds are richin bioactive compounds, including phenolic compounds, tannins, saponins, flavonoids, carbohydrates, proteins, aminoacids, lipids, ascorbic acid, and enzymes. However, the hepatoprotective effects have not been extensively studied usingadditional experimental models. Moreover, there is a lack of systematic and scientific analysis regarding their potentialrenal effects in the existing literature.
Regulatory Landscape and Quality Assurance in Nutraceutical Development Kratika Daniel, Vivek Daniel, Pooja Solanki, Arti Solanki Nutraceuticals and Obesity Strategies for Weight Management, 2025 This chapter deals with the role of nutraceuticals in weight management and lifestyle modification for long-term weight loss maintenance. Nutraceuticals are natural or synthetic products that have health benefits that can modulate various biological processes related to weight management, such as obesity, basic metabolic rate, hormonal disorders, stress, etc. A lifestyle modification program can fight with many diseases and disorders. Such type of lifestyle program includes dietary, physical, and emotional challenges to help individuals achieve their goal and sustain for a long term to manage a healthy and balanced life. Lifestyle modification is the first approachable treatment to manage BMR and weight. The main features of lifestyle modification programs are calorie-deficit diet, increased physical activities, and cognitive therapy. Nowadays, there are so many artificial intelligence (AI) tools or mobile applications, such as HealthifyMe, Fitelo, etc., available to maintain the lifestyle and weight. This chapter aims to review all perspectives on how combinations of nutraceuticals and lifestyle modification programs can act synergistically on BMR and weight management.
An Overview on Use of Artificial Intelligence Tools-A Boon or Curse for Academic Writing in Pharmaceutical Sectors Pharma Times, 2024
Preparation of Mouth Dissolving Sublingual Film of Fixed Dose Combination of Hydrochlorothiazide and Losartan Potassium and Statistical Optimization of Film Attal . V, Daniel . K International Journal of Drug Delivery Technology, 2024 Fast-dissolving films (FDFs) are innovative dosage forms offering advantages such as improved patient compliance, rapid onset of action, precise dosing, and favorable taste. Particularly beneficial for patients unable to swallow or require immediate drug absorption, FDFs dissolve rapidly in the mouth, releasing medication directly into the oral cavity. The sublingual application enhances drug delivery due to the high permeability of the sublingual mucosa, bypassing hepatic first-pass metabolism. This route is advantageous for drugs like losartan potassium (LP) and hydrochlorothiazide (HCZ), which benefit from enhanced bioavailability and faster onset of action. Preformulation studies included UV spectroscopy for λmax determination (LP: 282 nm, HCZ: 250 nm), DSC for thermal analysis (LP: 289.11°C, HCZ: 104.76°C), and solubility studies confirming LP’s solubility in acidic pH and HCZ’s increased solubility in pH 6.7 buffer. Compatibility studies using FTIR and DSC validated stability with excipients. Formulation optimization with HPMC E15 resulted in films with characteristics like film weight (145.66–167.0 mg), thickness (0.246–0.326 mm), folding endurance (898.0–1274.0), pH (6.0–6.70), disintegration time (77.66–174.6 s), tensile strength (77.04–141.90 g/cm²), and drug release profiles (LP: 68.78–96.22%, HCZ: 59.49–95.43% at 21 minutes). In summary, this study successfully developed LP and HCZ FDFs with optimized characteristics, demonstrating their potential in hypertension management and improving therapeutic outcomes.
Formulation, Development and Optimization of Silymarin Loaded Nanoparticle Orodispersible Tablets for its Anti-inflammatory Activity Radhika N Kotame, Kratika . Daniel International Journal of Drug Delivery Technology, 2024 The objective of this work was to enhance the anti-inflammatory properties of silymarin by developing and refining an orodispersible tablet formulation loaded with nanoparticles. Formulation development and optimization of silymarin-loaded nanoparticle orodispersible tablet for its anti-inflammatory activity is the focus of this research paper. Nanoparticle drug delivery systems can help to overcome these limitations by enhancing solubility and absorption. Disintegration and dissolution of Orodispersible tablets take place in the mouth rapidly without water, providing a faster onset of action. These nanoparticles were then incorporated into orodispersible tablets containing superdisintegrants and sweeteners using direct compression. A Box-Behnken design was utilized to systematically optimize the tablet formulation based on responses including disintegration time, wetting time, hardness and friability. After 15 minutes, SL-NP Formulation -3, which contains 5% crospovidone as a superdisintegrant, demonstrated maximal drug release, or 98.5%. Rats with paw edema caused by carrageenan were used to test the anti-inflammatory potential. Following three hours, the 400 mg/kg dose showed a strong 48% inhibition; the impact grew to 52% after three hours. Overall, the development of silymarin nanoparticle orodispersible tablets shows promise for improving the anti-inflammatory effects of silymarin through a synergistic combination of nanoencapsulation and rapid disintegrating tablet technologies.
In-vitro and In-silico Docking Studies of Active Constituents of Momordica charantia and Emblica officinalis as Potential Alpha-Amylase Inhibitors Kratika Daniel, Vivek Daniel, Charanjeet Singh, Anil K Gupta, Yashwant Daniel International Journal of Pharmaceutical Quality Assurance, 2024 Introduction: Commercially available oral hypoglycemic drugs are known to be potential alpha-amylase inhibitors that reduce postprandial hyperglycemia. Natural drugs are nowadays very popular in the treatment of diabetes. Aim and Objectives: This study investigated in-vitro alpha-amylase inhibition of Momordica charantia and Emblica officinalis and studied the interaction between active phytoconstituents and alpha-amylase enzyme. Method: Active constituents of both fruits were identified, docking studies were performed using Autodock Vina, and interactions were studied using PyMOL and Discovery Studio. The alpha-amylase inhibitory potentials of the fresh juice were investigated at the concentration of the fresh juice with alpha amylase enzyme and starch solution at 565 nm was observed. Result: With the docking studies, it was observed that momoridicin I and II showed better interaction with alpha-amylase enzyme (PDB ID: 1B2Y) and showed binding energies at -8.2 and -8.4 Kcal/mol, respectively. The fresh juice of both fruits showed the most effective alpha-amylase inhibition and IC50 values found at 440 and 312 μL/mL, respectively. Summary and Conclusion: The attempt to study in-silico docking studies and in-vitro alpha-amylase enzyme inhibition were successfully performed. Comparatively, M. charantia showed more enzyme inhibition at low concentrations.
Estimation of Active Components in Gokshura Tablet and Pushyanug Churna Formulation using High-performance Thin Layer Chromatography Method Priyanka . Rathore, Kratika . Daniel, Vivek . Daniel, Charanjeet . Singh, Yashwant . ., Anil K. Gupta International Journal of Drug Delivery Technology, 2022 Gokshura tablet is an ayurvedic formulation with gokhru (Tribulus terrestris) as best fixing recommended for building vitality levels. It enhances life, sexual want and drive. Pushyanug churna is an ayurvedic polyherbal formulation, hence this seems essential to explain the material institutionalization, various bioactive markers, blends exhibit in the polyherbal ayurveda compositions such as pushyanug churna. Point of the exhibit effort has been to create what’s more, approve a high-performance thin layer chromatography (HPTLC) strategy for assurance of diosgenin present in gokshura tablet. Mangiferin and chlorogenic acid are present in pushyanug churna. Diosgenin, a biomarker chemical found in gokshura tablets, and mangiferin, a biomarker compound found in pushyanug churna, were standardized using recently developed easy and accurate HPTLC procedures. Pre-coated silica gel 60-F254 was employed at the stationary phase and a mixture of toluene, ethyl acetate, and formic acid (in the proportions 5:4:1) was employed for the mobile phase in the development methodology for diosgenin. In the mobile phase, mangiferin, ethyl acetate and methanol were added in a ratio of 40:60 v/v were utilized. In the chlorogenic acid mobile phase, ethyl acetate:formic acid:acetic acid:water (10:1.1:1.1:2.6 v/v). It was determined that the Rf value of the marker chemical was 0.77 (diosgenin) in gokhsura tablet and 0.23 mangiferin, 0.75 chlorogenic acid in pushyanug churna. For bioactive marker chemicals found in in-house and commercially available formulations, the developed HPTLC approach has shown to be straightforward, sensitive, specific, and dependable.
In silico docking studies, synthesis and chacterization of some novel 1,3,4 thiadiazole analogues of 4-amino hippuric acid as potent antimicrobial agents Indian Drugs, 2018
Short notes in-silico preliminary docking screening of some anti-Alzheimer drugs Indian Drugs, 2016
Pharmacognostical and phytochemical studies on stamens of Mesua ferrea as potent anthelmintic agent Indian Drugs, 2015
Evaluation of wound healing effect of a polyherbal formulation by different cutaneous wound models Pharmacologyonline, 2011
Synthesis and antihelmentic activity of some azole derivative of hippuric acid International Journal of Pharmaceutical Sciences, 2010
Synthesis and In-vitro anthelmintic activity of some 4-aminophenol derivatives International Journal of Pharmaceutical Research, 2010
Synthesis and biological activities of some novel 1,2,4-triazole [3,4-B][1,3,4] thiadiazole derivatives Journal of Global Pharma Technology, 2010
