Takumi Nishiuchi

Scopus Publications

Paleoproteomics of pottery food residues reveals plant cupin superfamily proteins preserved for thousands of years
Takumi Nishiuchi, Masataka Nakano, Shinya Ohki, Kenichi Kobayashi, Guoping Sun, Joonho Son, Shinya Shoda
Iscience, 2026
, were detected in the charred food residues. Lipid and isotope data were consistent with the proteomic results. Furthermore, 3D protein structure mapping showed that the detected peptides fall within β-barrel regions of the proteins, the structurally stable cores of these seed storage proteins. Thus, pottery foodcrusts preserved plant cupin proteins over 7,900-2,500 years.
Omics Profiles of the Null Segregants of RNA-Directed DNA Methylation-Positive Tobacco Plants
Haruka Morimoto, Yukiko Umeyama, Sayaka Hirai, Takumi Nishiuchi, Takumi Ogawa, Tomofumi Mochizuki, Daisaku Ohta, Hiroaki Kodama, Taira Miyahara
Agronomy, 2026
RNA-directed DNA methylation (RdDM), a new plant breeding technology, induces epigenetic modifications that can be inherited even after segregation of the responsible transgene. The transgene-free descendants (null segregants) are potentially exempt from the regulation of genetically modified plants. To evaluate the risks of potential unintended molecular changes in the null segregants of RdDM-positive plants, we produced null segregants (S44end2-null) from a transgenic tobacco line in which RdDM targeting the promoter of the transgene was introduced. Comprehensive multi-omics analyses, including transcriptomics, proteomics, and metabolomics, were conducted using S44end2-null and wild-type (WT) plants. Principal component analysis demonstrated clear separation of the transcriptomic and proteomic profiles of the two groups. The metabolomic profiles of S44end2-null plants exhibited considerable overlap with those of WT plants. Proteomic analysis of the null segregants of tobacco plants transformed with an empty vector demonstrated distinct cluster separation from WT plants. Because only sporadic DNA methylation on the tobacco genome was expected by the RdDM construct used in this study, the observed differences in omics profiles are considered to be significantly influenced by genetic variation accumulated during the transformation and regeneration processes (somaclonal variation). The safety assessment points for null segregants using RdDM technology are discussed.
Protocol to pinpoint oxidized and reduced cysteine residues in brown fat using differential alkylation labeling techniques
Hein Ko Oo, Cynthia M. Galicia-Medina, Takumi Nishiuchi, Hiroaki Takayama, Toshinari Takamura
STAR Protocols, 2025
Cysteine post-translational modifications are important for protein functions and protein-protein interactions. Here, we present a protocol to detect the reversibly oxidized and reduced cysteine residues of proteins using differential alkylation labeling techniques with liquid chromatography-mass spectrometry (LC-MS) analysis. We describe steps for tissue sample preparation, differential alkylation, trypsin digestion, and LC-MS analysis. We then detail procedures for protein identification and data analysis. This protocol has potential application in pinpointing the modified cysteine residues in organs and cells in various disease models. For complete details on the use and execution of this protocol, please refer to Oo et al. 1 • Steps for protein extraction from mouse tissues • Instructions for the double alkylation of cysteine residues • Labeling of cysteine residues based on their redox status • Steps to organize the oxidized and reduced cysteine residues from the LC-MS output Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cysteine post-translational modifications are important for protein functions and protein-protein interactions. Here, we present a protocol to detect the reversibly oxidized and reduced cysteine residues of proteins using differential alkylation labeling techniques with liquid chromatography-mass spectrometry (LC-MS) analysis. We describe steps for tissue sample preparation, differential alkylation, trypsin digestion, and LC-MS analysis. We then detail procedures for protein identification and data analysis. This protocol has potential application in pinpointing the modified cysteine residues in organs and cells in various disease models.
Cysteine redoxome landscape in the liver of male mice fed a high-fat high-sucrose diet
Cynthia M. Galicia-Medina, Hein Ko Oo, Takumi Nishiuchi, Ryota Tanida, Tuerdiguli Abuduyimiti, Hisanori Goto, Yujiro Nakano, Yumie Takeshita, Kiyo-aki Ishii, Takashi Toyama, Yoshiro Saito, Hiroaki Takayama, Toshinari Takamura
Journal of Biological Chemistry, 2025
Reversible cysteine posttranslational modifications serve as a "switch" for protein structure-function dynamics. Herein, we applied a comprehensive strategy to map the cysteine redoxome by pinpointing over 5000 oxidized and reduced cysteine residues in the liver of male mice fed either a normal chow diet or a high-fat/high-sucrose diet (HFHSD). The global and subcellular distribution of oxidized and reduced cysteine residues remained stable across both diet groups, indicating that HFHSD does not induce widespread shifts in cysteine redox equilibrium. Proteomic analyses revealed that HFHSD upregulates proteins involved in genomic stability, lipid detoxification, and energy regulation, while downregulating those linked to detoxification and metabolic flexibility. Notably, 169 cysteine residues exhibited dynamic redox changes in response to HFHSD, mapping to 35 Kyoto Encyclopedia of Genes and Genomes pathways central to redox balance and energy homeostasis. Motif and structural analyses demonstrated that the reactivity of cysteine residues sensitive to redox stress is dictated by distinct electrostatic microenvironments and subcellular localization. Cysteine residues sensitive to HFHSD-induced oxidation were enriched in mitochondria and cytosol, and cysteine residues sensitive to HFHSD-induced reduction in extracellular regions. Furthermore, cysteine residues sensitive to HFHSD-induced reduction mainly participate in disulfide bond formation and are exposed to the surface of the protein, suggesting roles as molecular switches in protein function. The current cysteine redoxome strategy broadens the disease-associated proteome landscape and provides potential therapeutic target cysteine residues critical for regulating protein functions and interactions relevant to pathophysiology.
Translocation of Insecticidal Bt Protein in Transgrafted Plants
Arisa Ando, Hitomi Ohkubo, Hisae Maki, Takumi Nishiuchi, Takumi Ogawa, Tomofumi Mochizuki, Daisaku Ohta, Hiroaki Kodama, Taira Miyahara
Biotech, 2025
Transgrafting constitutes a technique involving the integration of genetically modified (GM) and non-GM plant organisms. Typically, edible components derived from non-GM scions are categorized as non-GM food products, attributed to the absence of exogenous genetic material within their respective genomes. Non-GM food status could be compromised if proteins translocated across the graft interface. We investigated the movement of insecticidal Bacillus thuringiensis (Bt) crystal proteins, widely utilized in GM crop species. Tobacco plants engineered to express the Cry1Ab gene exhibited trace levels of Cry1Ab protein accumulation. In transgrafted plants, translocated Cry1Ab protein originating from GM rootstocks was detectable within scion foliar tissues but not within the seeds obtained from the non-GM scion. This result unequivocally demonstrates the capacity for Bt protein translocation from rootstocks to scions yet indicates a constrained distribution confined to scion tissues relatively close to the graft junction. While regulatory considerations necessitate a thorough appraisal of potential risks associated with Bt proteins, the results shown here facilitate the commercialization of the edible components as non-GM food products.
Vitamin D derivatives inhibit mesenchymal transition of mesothelial cells and mitigate peritoneal dissemination of ovarian cancer
Kazuhisa Fujita, Maia Hayashi, Masato Yoshihara, Satoshi Nomura, Kazuhisa Kitami, Emiri Miyamoto, Shohei Iyoshi, Kazumasa Mogi, Hiroki Fujimoto, Kaname Uno, Atsushi Kunishima, Yoshihiko Yamakita, Hiroyuki Tomita, Rino Tsutsumi, Ryota Sakamoto, Kazuo Nagasawa, Yusuke Masuo, Takumi Nishiuchi, Kiyosumi Shibata, Atsushi Enomoto, Hiroaki Kajiyama
Medical Molecular Morphology, 2025
Necrosis-Suppressing Effector Protein ChEC88 Adopts a Novel Structural Motif Conserved Among Genus-Spanning Hemibiotrophic Phytopathogens
Shinya Ohki, Hiroyuki Takahara, Tomohiro Imamura, Kosei Sakane, Asihan Bai, Kazunori Sasaki, Takumi Nishiuchi, Masashi Mori
Plants, 2025
Phytopathogenic fungi secrete numerous effector proteins to disrupt plant defenses. At present, their sequence–structure–function relationships remain poorly understood owing to their diversity. Comprehensive understanding of conserved effectors is necessary to elucidate the molecular relationship between fungi and plants. To fill this research gap, we investigated the Colletotrichum higginsianum effector candidate (ChEC)-88 specifically expressed during infection. Notably, similar to the biotrophy-associated secreted protein 3 (BAS3) from Pyricularia oryzae, ChEC88 inhibited plant cell death caused by necrosis- and ethylene-inducing peptide 1-like protein (NLP1). Nuclear magnetic resonance analysis results revealed that ChEC88 adopted a novel pseudo two-fold symmetrical three-dimensional structure. Homology modeling suggested that BAS3 exhibited a ChEC88-like conformation despite sharing less than 50% sequence identity. Through PSI-BLAST searches, we found that ChEC88 homologs were conserved in various hemibiotrophic phytopathogenic fungi, including Colletotrichum, P. oryzae, and Fusarium species. Functional assays demonstrated that all of the representative homologs suppressed NLP1-induced plant cell death. Mutation experiments identified the residues critical for ChEC88 function. Overall, our findings suggest that hemibiotrophic phytopathogenic fungi share a conserved immune-suppression strategy mediated by ChEC88-like proteins and that such effectors possibly originated from a common ancestral lineage of phytopathogenic fungi.
Complement component C4a binds to oxytocin and modulates plasma oxytocin concentrations and social behavior in male mice
Yasuhiko Yamamoto, Anpei Zhang, Teruko Yuhi, Ai Harashima, Anna A. Shabalova, Emina Nishizawa, Michiru Moriya, Seiichi Munesue, Yu Oshima, Maria Gerasimenko, Kazumi Furuhara, Chiharu Tsuji, Shigeru Yokoyama, Shinichi Horike, Jumpei Terakawa, Takiko Daikoku, Takumi Nishiuchi, Katsuya Sakai, Haruhiro Higashida
Biochemical and Biophysical Research Communications, 2025
Oxytocin (OT) is a hormone with a short half-life that is released from the posterior pituitary gland into the bloodstream. It plays an important role in childbirth, breastfeeding, and social behavior in humans and animals. However, the endogenous OT system, including plasma OT pharmacokinetics, is not fully understood. In this study, we used a click chemistry probe to discover a novel OT-binding protein in human serum and identified C4a, a peptidase-cleaved fragment of complement component 4 (C4). A direct association between OT and C4a expression was also confirmed. Upon knocking out Slp gene, which is one of the two mouse C4 genes, the level of free form of plasma OT was higher in Slp knockout ( Slp -/- ) mice than in wild-type ( Slp +/+ ) mice after intraperitoneal OT injection. In addition, open-field tests revealed that social interactions were higher in Slp -/- mice than Slp +/+ mice. An in vitro blood-brain barrier model showed that C4a neither inhibited nor accelerated receptor for advanced glycation end-product (RAGE)-dependent brain transport in OT. Our data validate the novel concept that C4a with OT-binding capacity can alter the dynamics of the free form of OT concentrations in the plasma, which may disturb the availability of OT to the brain, resulting in an interruption of OT-associated social behavior.
Mechanism of antithrombin deficiency due to the novel variant C32W in the C-terminus of the signal peptide
Yuika Kikuchi, Satomi Nagaya, Tomoki Togashi, Yuta Imai, Maki Togashi, Yuhei Araiso, Takumi Nishiuchi, Eriko Morishita
International Journal of Hematology, 2025
Dietary bacterial cellulose modulates gut microbiota and increases bile acid excretion in high-fat diet-induced obese mice
Takao Nagano, Aaron Lelo Pambu, Yasuki Higashimura, Masataka Nakano, Takumi Nishiuchi
Food Hydrocolloids for Health, 2025
Bacterial cellulose (BC), with its unique and highly viscous nanofiber structure, was the focus of this study. We investigated BC supplementation's potential for body weight management and its effects on gut microbiota and bile acid metabolism in high-fat diet (HFD)-fed mice. Mice were categorized into four groups: BC-untreated group on a control-fat diet (CF), BC-untreated group on an HFD (UHF), medium BC-treated group on an HFD (MHF), and high BC-treated group on an HFD (HHF). Over 12 weeks, BC supplementation significantly reduced body weight gain, fat accumulation , and plasma total cholesterol levels compared to the UHF group. Metabolomic analysis revealed that BC intake upregulated the bile acid biosynthesis pathway. The fecal analysis confirmed that bile acid excretion was highest in the HHF group, with increased plasma bile acid levels. BC's bile acid-binding capacity was 3.2 times greater than that of cellulose, contributing to these metabolic effects. Gut microbiota analysis showed that BC increased beneficial bacteria, including Bacteroides and Ruminococcus , which are involved in polysaccharide metabolism. However, BC intake did not enhance short-chain fatty acid production. These findings highlight BC's potential as a dietary intervention for obesity and cholesterol management through its effects on bile acid metabolism and gut microbiota.
High-viscosity dietary fibers modulate gut microbiota and liver metabolism to prevent obesity in high-fat diet-fed mice
Takao Nagano, Yasuki Higashimura, Masataka Nakano, Takumi Nishiuchi, Aaron Pambu Lelo
International Journal of Biological Macromolecules, 2025
Cysteine redoxome landscape in mouse brown adipose tissue under acute cold exposure
Hein Ko Oo, Cynthia M. Galicia-Medina, Takumi Nishiuchi, Ryota Tanida, Hisanori Goto, Yujiro Nakano, Yumie Takeshita, Yoshiro Saito, Hiroaki Takayama, Toshinari Takamura
Iscience, 2025
Trideoxycytidine Diphosphate Promotes Neural Stem Cell Proliferation and Neurogenesis in Mice
Takahiro Ishimoto, Fuyu Hayashi, Yuya Yamamoto, Keisuke Kiriyama, Reiya Yamashita, Naoto Matsumura, Takumi Nishiuchi, Yusuke Masuo, Mica Fujita, Keita Sutoh, Yukio Kato
Journal of Nutrition, 2025
Palaeoproteomic investigation of an ancient human skeleton with abnormal deposition of dental calculus
Yoko Uchida-Fukuhara, Shigeru Shimamura, Rikai Sawafuji, Takumi Nishiuchi, Minoru Yoneda, Hajime Ishida, Hirofumi Matsumura, Takumi Tsutaya
Scientific Reports, 2024
Dual inhibition of SUMOylation and MEK conquers MYC-expressing KRAS-mutant cancers by accumulating DNA damage
Hiroshi Kotani, Hiroko Oshima, Justin C. Boucher, Tomoyoshi Yamano, Hiroyuki Sakaguchi, Shigeki Sato, Koji Fukuda, Akihiro Nishiyama, Kaname Yamashita, Koushiro Ohtsubo, Shinji Takeuchi, Takumi Nishiuchi, Masanobu Oshima, Marco L. Davila, Seiji Yano
Journal of Biomedical Science, 2024
Comprehensive antitumor immune response boosted by dual inhibition of SUMOylation and MEK in MYC-expressing KRAS-mutant cancers
Hiroshi Kotani, Tomoyoshi Yamano, Justin C. Boucher, Shigeki Sato, Hiroyuki Sakaguchi, Koji Fukuda, Akihiro Nishiyama, Kaname Yamashita, Koushiro Ohtsubo, Shinji Takeuchi, Takumi Nishiuchi, Hiroko Oshima, Masanobu Oshima, Marco L. Davila, Seiji Yano
Experimental Hematology and Oncology, 2024
Proteomic Analysis to Understand the Promotive Effect of Ethanol on Soybean Growth Under Salt Stress
Setsuko Komatsu, Takumi Nishiuchi
Biology, 2024
Super-enhancer trapping by the nuclear pore via intrinsically disordered regions of proteins in squamous cell carcinoma cells
Masaharu Hazawa, Dini Kurnia Ikliptikawati, Yuki Iwashima, De-Chen Lin, Yuan Jiang, Yujia Qiu, Kei Makiyama, Koki Matsumoto, Akiko Kobayashi, Goro Nishide, Lim Keesiang, Hironori Yoshino, Toshinari Minamoto, Takeshi Suzuki, Isao Kobayashi, Makiko Meguro-Horike, Yan-Yi Jiang, Takumi Nishiuchi, Hiroki Konno, H. Phillip Koeffler, Kazuyoshi Hosomichi, Atsushi Tajima, Shin-ichi Horike, Richard W. Wong
Cell Chemical Biology, 2024
Wet-type grinder-treated okara modulates gut microbiota composition and attenuates obesity in high-fat-fed mice
Takao Nagano, Chihiro Watanabe, Eri Oyanagi, Hiromi Yano, Takumi Nishiuchi
Food Research International, 2024
Identification of uromodulin deposition in the stroma of perinephric fibromyxoid nephrogenic adenoma by mass spectrometry
Kaori Yoshimura, Yukinobu Ito, Mina Suzuki, Masafumi Horie, Takumi Nishiuchi, Yukako Shintani‐Domoto, Kazuyoshi Shigehara, Hiroko Oshima, Masanobu Oshima, Akiteru Goto, Takayuki Nojima, Toyonori Tsuzuki, Atsushi Mizokami, Hiroko Ikeda, Daichi Maeda
Pathology International, 2024
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration
Ketty C. Tamburrini, Sayo Kodama, Sacha Grisel, Mireille Haon, Takumi Nishiuchi, Bastien Bissaro, Yasuyuki Kubo, Sonia Longhi, Jean-Guy Berrin
Proceedings of the National Academy of Sciences of the United States of America, 2024
Proteomic and molecular analyses to understand the promotive effect of safranal on soybean growth under salt stress
Rehana Kausar, Takumi Nishiuchi, Setsuko Komatsu
Journal of Proteomics, 2024
Millmeter-wave irradiation regulates mRNA-expression and the ubiquitin-proteasome system in wheat exposed to flooding stress
Setsuko Komatsu, Takumi Nishiuchi, Takashi Furuya, Masahiko Tani
Journal of Proteomics, 2024
Translocation of green fluorescent protein in homo-and hetero-transgrafted plants
Takumi Ogawa, Kanae Kato, Harue Asuka, Yumi Sugioka, Tomofumi Mochizuki, Hirokazu Fukuda, Takumi Nishiuchi, Taira Miyahara, Hiroaki Kodama, Daisaku Ohta
Plant Biotechnology, 2024
Renal Pharmacokinetic Adaptation to Cholestasis Causes Increased Nephrotoxic Drug Accumulation by Mrp6 Downregulation in Mice
Hiroshi Arakawa, Takumi Kawanishi, Dai Shengyu, Takumi Nishiuchi, Makiko Meguro-Horike, Shin-ichi Horike, Masahiro Sugimoto, Yukio Kato
Journal of Pharmaceutical Sciences, 2023
Potential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: a cross-sectional case–control study
Kohei Ogura, Maho Endo, Takashi Hase, Hitomi Negami, Kohsuke Tsuchiya, Takumi Nishiuchi, Takeshi Suzuki, Kazuhiro Ogai, Hiromi Sanada, Shigefumi Okamoto, Junko Sugama
Clinical Proteomics, 2023
Kynurenine promotes Calcitonin secretion and reduces cortisol in the Japanese flounder Paralichthys olivaceus
Takahiro Ikari, Yukihiro Furusawa, Yoshiaki Tabuchi, Yusuke Maruyama, Atsuhiko Hattori, Yoichiro Kitani, Kenji Toyota, Arata Nagami, Jun Hirayama, Kazuki Watanabe, Atsushi Shigematsu, Muhammad Ahya Rafiuddin, Shouzo Ogiso, Keisuke Fukushi, Kohei Kuroda, Kaito Hatano, Toshio Sekiguchi, Ryotaro Kawashima, Ajai K. Srivastav, Takumi Nishiuchi, Akihiro Sakatoku, Masa-aki Yoshida, Hajime Matsubara, Nobuo Suzuki
Scientific Reports, 2023
An agroecological structure model of compost—soil—plant interactions for sustainable organic farming
Hirokuni Miyamoto, Katsumi Shigeta, Wataru Suda, Yasunori Ichihashi, Naoto Nihei, Makiko Matsuura, Arisa Tsuboi, Naoki Tominaga, Masahiko Aono, Muneo Sato, Shunya Taguchi, Teruno Nakaguma, Naoko Tsuji, Chitose Ishii, Teruo Matsushita, Chie Shindo, Toshiaki Ito, Tamotsu Kato, Atsushi Kurotani, Hideaki Shima, Shigeharu Moriya, Satoshi Wada, Sankichi Horiuchi, Takashi Satoh, Kenichi Mori, Takumi Nishiuchi, Hisashi Miyamoto, Hiroaki Kodama, Masahira Hattori, Hiroshi Ohno, Jun Kikuchi, Masami Yokota Hirai
Isme Communications, 2023
Apple-derived extracellular vesicles modulate the expression of human intestinal bile acid transporter ASBT/SLC10A2 via downregulation of transcription factor RARα
Shinya Usui, Qiunan Zhu, Hisakazu Komori, Yui Iwamoto, Takumi Nishiuchi, Yoshiyuki Shirasaka, Ikumi Tamai
Drug Metabolism and Pharmacokinetics, 2023
Postnatal expression of CD38 in astrocytes regulates synapse formation and adult social memory
Tsuyoshi Hattori, Stanislav M Cherepanov, Ryo Sakaga, Jureepon Roboon, Dinh Thi Nguyen, Hiroshi Ishii, Mika Takarada‐Iemata, Takumi Nishiuchi, Takayuki Kannon, Kazuyoshi Hosomichi, Atsushi Tajima, Yasuhiko Yamamoto, Hiroshi Okamoto, Akira Sugawara, Haruhiro Higashida, Osamu Hori
EMBO Journal, 2023
Membrane Proteomics to Understand Enhancement Effects of Millimeter-Wave Irradiation on Wheat Root under Flooding Stress
Setsuko Komatsu, Kazuna Hamada, Takashi Furuya, Takumi Nishiuchi, Masahiko Tani
International Journal of Molecular Sciences, 2023
Cell assembly analysis of neural circuits for innate behavior in Drosophila melanogaster using an immediate early gene stripe/egr-1
Seika Takayanagi-Kiya, Natsumi Shioya, Takumi Nishiuchi, Masafumi Iwami, Taketoshi Kiya
Proceedings of the National Academy of Sciences of the United States of America, 2023
Tandem metalloenzymes gate plant cell entry by pathogenic fungi
Bastien Bissaro, Sayo Kodama, Takumi Nishiuchi, Anna Maria Díaz-Rovira, Hayat Hage, David Ribeaucourt, Mireille Haon, Sacha Grisel, A. Jalila Simaan, Fred Beisson, Stephanie M. Forget, Harry Brumer, Marie-Noëlle Rosso, Victor Guallar, Richard O’Connell, Mickaël Lafond, Yasuyuki Kubo, Jean-Guy Berrin
Science Advances, 2022
Reduced graphene oxide increases cells with enlarged outer membrane of Citrifermentans bremense and exopolysaccharides secretion
Lingyu Meng, Li Xie, Yuu Hirose, Takumi Nishiuchi, Naoko Yoshida
Biosensors and Bioelectronics, 2022
Proteomic Profiling of Plant and Pathogen Interaction on the Leaf Epidermis
Yasir Sidiq, Daisuke Tamaoki, Takumi Nishiuchi
International Journal of Molecular Sciences, 2022
Estrogen induces the expression of EBV lytic protein ZEBRA, a marker of poor prognosis in nasopharyngeal carcinoma
Hirotomo Dochi, Satoru Kondo, Takayuki Murata, Masaki Fukuyo, Asuka Nanbo, Kousho Wakae, Wen‐Ping Jiang, Toshihide Hamabe‐Horiike, Mariko Tanaka, Takumi Nishiuchi, Harue Mizokami, Makiko Moriyama‐Kita, Eiji Kobayashi, Nobuyuki Hirai, Takeshi Komori, Takayoshi Ueno, Yosuke Nakanishi, Miyako Hatano, Kazuhira Endo, Hisashi Sugimoto, Naohiro Wakisaka, Shin‐Hun Juang, Masamichi Muramatsu, Atsushi Kaneda, Tomokazu Yoshizaki
Cancer Science, 2022
Dehalococcoides mccartyi NIT01, a novel isolate, dechlorinates high concentrations of chloroethenes by expressing at least six different reductive dehalogenases
Masaki Asai, Naoko Yoshida, Toshiya Kusakabe, Mohamed Ismaeil, Takumi Nishiuchi, Arata Katayama
Environmental Research, 2022
Subcellular proteomics to understand promotive effect of plant-derived smoke solution on soybean root
Yusuke Murashita, Takumi Nishiuchi, Shafiq Ur Rehman, Setsuko Komatsu
Proteomes, 2021
Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola
Ayumi Kosaka, Marta Pastorczyk, Mariola Piślewska-Bednarek, Takumi Nishiuchi, Erika Ono, Haruka Suemoto, Atsushi Ishikawa, Henning Frerigmann, Masanori Kaido, Kazuyuki Mise, Paweł Bednarek, Yoshitaka Takano
Scientific Reports, 2021
Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system
Marie Shinohara, Hiroshi Arakawa, Yuuichi Oda, Nobuaki Shiraki, Shinji Sugiura, Takumi Nishiuchi, Taku Satoh, Keita Iino, Sylvia Leo, Yusuke Kato, Karin Araya, Takumi Kawanishi, Tomoki Nakatsuji, Manami Mitsuta, Kosuke Inamura, Tomomi Goto, Kenta Shinha, Wataru Nihei, Kikuo Komori, Masaki Nishikawa, Shoen Kume, Yukio Kato, Toshiyuki Kanamori, Yasuyuki Sakai, Hiroshi Kimura
Scientific Reports, 2021
Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59
Suthitar Singkaravanit‐Ogawa, Ayumi Kosaka, Saeko Kitakura, Kotaro Uchida, Takumi Nishiuchi, Erika Ono, Satoshi Fukunaga, Yoshitaka Takano
Plant Journal, 2021
Nicotinamide effectively suppresses fusarium head blight in wheat plants
Yasir Sidiq, Masataka Nakano, Yumi Mori, Takashi Yaeno, Makoto Kimura, Takumi Nishiuchi
International Journal of Molecular Sciences, 2021
Induction of tocopherol biosynthesis through heat shock treatment in Arabidopsis
Miyuki Kusajima, Moeka Fujita, Takumi Nishiuchi, Hideo Nakashita, Tadao Asami
Bioscience Biotechnology and Biochemistry, 2021
Nicotinamide mononucleotide potentiates resistance to biotrophic invasion of fungal pathogens in barley
Kana Ueda, Yuichi Nakajima, Hiroshi Inoue, Kappei Kobayashi, Takumi Nishiuchi, Makoto Kimura, Takashi Yaeno
International Journal of Molecular Sciences, 2021
Abnormal social behavior and altered gene expression in mice lacking NDRG2
Mika Takarada-Iemata, Toru Yoshihara, Nahoko Okitani, Keiko Iwata, Tsuyoshi Hattori, Hiroshi Ishii, Jureepon Roboon, Dinh Thi Nguyen, Qiyan Fan, Takashi Tamatani, Takumi Nishiuchi, Masahide Asano, Osamu Hori
Neuroscience Letters, 2021
Characterization of mycosporine-like amino acids in the edible cyanobacterium Nostoc commune (Di Pi Cai) from China
Yang Wei, Takumi Nishiuchi, Toshio Sakamoto
Journal of General and Applied Microbiology, 2021
Proteasomal degradation of polycomb-group protein CBX6 confers MMP-2 expression essential for mesothelioma invasion
Katsuya Sakai, Takumi Nishiuchi, Shoichiro Tange, Yoshinori Suzuki, Seiji Yano, Minoru Terashima, Takeshi Suzuki, Kunio Matsumoto
Scientific Reports, 2020
Impact of nitrogen metabolism-associated culture pH changes on regulation of Fusarium trichothecene biosynthesis: revision of roles of polyamine agmatine and transcription factor AreA
Yuichi Nakajima, Manami Akasaka, Takuya Shiobara, Yoshiyuki Kitou, Kazuyuki Maeda, Kyoko Kanamaru, Shuichi Ohsato, Tetsuo Kobayashi, Takumi Nishiuchi, Makoto Kimura
Current Genetics, 2020
Amplification and cloning of arabidopsis 6xhis-tagged mpk6 fusion encoded gene to characterize biochemical mitogen-activated protein kinase in disease resistance role against Fusarium graminearum
M H Rahmah, T Nishiuchi
Iop Conference Series Earth and Environmental Science, 2020
Arabidopsis SMN2/HEN2, encoding DEAD-Box RNA helicase, governs proper expression of the resistance gene SMN1/RPS6 and is involved in dwarf, autoimmune phenotypes of mekk1 and mpk4 Mutants
Momoko Takagi, Naoki Iwamoto, Yuta Kubo, Takayuki Morimoto, Hiroki Takagi, Fuminori Takahashi, Takumi Nishiuchi, Keisuke Tanaka, Teruaki Taji, Hironori Kaminaka, Kazuo Shinozaki, Kazuya Akimitsu, Ryohei Terauchi, Ken Shirasu, Kazuya Ichimura
Plant and Cell Physiology, 2020
Substrate specificities of Fusarium biosynthetic enzymes explain the genetic basis of a mixed chemotype producing both deoxynivalenol and nivalenol-type trichothecenes
Kazuyuki Maeda, Yuya Tanaka, Momoko Matsuyama, Masayuki Sato, Kazuki Sadamatsu, Tamotsu Suzuki, Kosuke Matsui, Yuichi Nakajima, Takeshi Tokai, Kyoko Kanamaru, Shuichi Ohsato, Tetsuo Kobayashi, Makoto Fujimura, Takumi Nishiuchi, Naoko Takahashi-Ando, Makoto Kimura
International Journal of Food Microbiology, 2020
Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila
Saori Nonaka, Emil Salim, Koki Kamiya, Aki Hori, Firzan Nainu, Rangga Meidianto Asri, Ayu Masyita, Takumi Nishiuchi, Shoji Takeuchi, Noriyuki Kodera, Takayuki Kuraishi
Frontiers in Immunology, 2020
Heterochromatin protein 1γ deficiency decreases histone H3K27 methylation in mouse neurosphere neuronal genes
Chie Naruse, Kanae Abe, Toru Yoshihara, Tomoaki Kato, Takumi Nishiuchi, Masahide Asano
FASEB Journal, 2020
Arabidopsis MAPKKK δ-1 is required for full immunity against bacterial and fungal infection
Tomoya Asano, Thi Hang-Ni Nguyen, Michiko Yasuda, Yasir Sidiq, Kohji Nishimura, Hideo Nakashita, Takumi Nishiuchi
Journal of Experimental Botany, 2020
Comparative analysis of the effect of inorganic and organic chemicals with silver nanoparticles on soybean under flooding stress
Takuya Hashimoto, Ghazala Mustafa, Takumi Nishiuchi, Setsuko Komatsu
International Journal of Molecular Sciences, 2020
Melatonin is a potential drug for the prevention of bone loss during space flight
Mika Ikegame, Atsuhiko Hattori, Makoto J. Tabata, Kei‐ichiro Kitamura, Yoshiaki Tabuchi, Yukihiro Furusawa, Yusuke Maruyama, Tatsuki Yamamoto, Toshio Sekiguchi, Risa Matsuoka, Taizo Hanmoto, Takahiro Ikari, Masato Endo, Katsunori Omori, Masaki Nakano, Sayaka Yashima, Sadakazu Ejiri, Toshiki Taya, Hiroshi Nakashima, Nobuaki Shimizu, Masahisa Nakamura, Takashi Kondo, Kazuichi Hayakawa, Ichiro Takasaki, Atsushi Kaminishi, Ryosuke Akatsuka, Yuichi Sasayama, Takumi Nishiuchi, Masayuki Nara, Hachiro Iseki, Vishwajit S. Chowdhury, Shigehito Wada, Kenichi Ijiri, Toshio Takeuchi, Tohru Suzuki, Hironori Ando, Kouhei Matsuda, Masanori Somei, Hiroyuki Mishima, Yuko Mikuni‐Takagaki, Hisayuki Funahashi, Akihisa Takahashi, Yoshinari Watanabe, Masahiro Maeda, Hideaki Uchida, Akio Hayashi, Akira Kambegawa, Azusa Seki, Sachiko Yano, Toru Shimazu, Hiromi Suzuki, Jun Hirayama, Nobuo Suzuki
Journal of Pineal Research, 2019
Retinoblastoma inactivation induces a protumoral microenvironment via enhanced CCL2 secretion
Fengkai Li, Shunsuke Kitajima, Susumu Kohno, Akiyo Yoshida, Shoichiro Tange, Soichiro Sasaki, Nobuhiro Okada, Yuuki Nishimoto, Hayato Muranaka, Naoko Nagatani, Misa Suzuki, Sayuri Masuda, Tran C. Thai, Takumi Nishiuchi, Tomoaki Tanaka, David A. Barbie, Naofumi Mukaida, Chiaki Takahashi
Cancer Research, 2019
Plant pathogenic fungi Colletotrichum and Magnaporthe share a common G 1 phase monitoring strategy for proper appressorium development
Fumi Fukada, Sayo Kodama, Takumi Nishiuchi, Naoki Kajikawa, Yasuyuki Kubo
New Phytologist, 2019
Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis
Takuya Shiobara, Yuichi Nakajima, Kazuyuki Maeda, Manami Akasaka, Yoshiyuki Kitou, Kyoko Kanamaru, Shuichi Ohsato, Tetsuo Kobayashi, Takumi Nishiuchi, Makoto Kimura
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2019
Colletotrichum orbiculare MTF4 is a key transcription factor downstream of mor essential for plant signal-dependent appressorium development and pathogenesis
Sayo Kodama, Takumi Nishiuchi, Yasuyuki Kubo
Molecular Plant Microbe Interactions, 2019
Four chemotypes of the terrestrial cyanobacterium Nostoc commune characterized by differences in the mycosporine-like amino acids
Toshio Sakamoto, Akane Hashimoto, Minami Yamaba, Naoki Wada, Takayuki Yoshida, Kaori Inoue‐Sakamoto, Takumi Nishiuchi, Seiichi Matsugo
Phycological Research, 2019
Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist
Wenyu Miao, Katsuya Sakai, Naoya Ozawa, Takumi Nishiuchi, Yoshinori Suzuki, Kenichiro Ito, Tomomi Morioka, Masataka Umitsu, Junichi Takagi, Hiroaki Suga, Kunio Matsumoto
Scientific Reports, 2018
N-myc downstream-regulated gene 2 protects blood–brain barrier integrity following cerebral ischemia
Mika Takarada‐Iemata, Akifumi Yoshikawa, Hieu Minh Ta, Nahoko Okitani, Takumi Nishiuchi, Yasuhiro Aida, Tomoya Kamide, Tsuyoshi Hattori, Hiroshi Ishii, Takashi Tamatani, Thuong Manh Le, Jureepon Roboon, Yasuko Kitao, Tomohiro Matsuyama, Mitsutoshi Nakada, Osamu Hori
GLIA, 2018
Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum
Kazuyuki Maeda, Hinayo Ichikawa, Yuichi Nakajima, Takayuki Motoyama, Shuichi Ohsato, Kyoko Kanamaru, Tetsuo Kobayashi, Takumi Nishiuchi, Hiroyuki Osada, Makoto Kimura
ACS Chemical Biology, 2018
Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages
Juanjuan Zhao, Yasuo Okamoto, Yuya Asano, Kazuhiro Ishimaru, Sho Aki, Kazuaki Yoshioka, Noriko Takuwa, Takashi Wada, Yutaka Inagaki, Chiaki Takahashi, Takumi Nishiuchi, Yoh Takuwa
Plos One, 2018
Ubiquitin chain specificities of E6AP E3 ligase and its HECT domain
Fuminori Kobayashi, Takumi Nishiuchi, Kento Takaki, Hiroki Konno
Biochemical and Biophysical Research Communications, 2018
3,4-dihydroxybenzalacetone and caffeic acid phenethyl ester induce preconditioning ER stress and autophagy in SH-SY5Y cells
Ryoichi Tomiyama, Ken Takakura, Shouhei Takatou, Thuong M. Le, Takumi Nishiuchi, Yutaka Nakamura, Tetsuya Konishi, Seiichi Matsugo, Osamu Hori
Journal of Cellular Physiology, 2018
The possible roles of AtERF71 in the defense response against the Fusarium graminearum
Fitri Yelli, Tomoaki Kato, Takumi Nishiuchi
Plant Biotechnology, 2018
Expression of barley glutathione s-transferase13 gene reduces accumulation of reactive oxygen species by trichothecenes and paraquat in arabidopsis plants
Ninik Nihayatul Wahibah, Tomokazu Tsutsui, Daisuke Tamaoki, Kazuhiro Sato, Takumi Nishiuchi
Plant Biotechnology, 2018
Characterization of extracellular matrix components from the desiccation-tolerant cyanobacterium Nostoc commune
Kaori Inoue-Sakamoto, Yasunori Tanji, Minami Yamaba, Takumi Natsume, Takuya Masaura, Tomoya Asano, Takumi Nishiuchi, Toshio Sakamoto
Journal of General and Applied Microbiology, 2018
Characterization of mycosporine-like amino acids in the cyanobacterium nostoc verrucosum
Kaori Inoue-Sakamoto, Ehsan Nazifi, Chieri Tsuji, Tomoya Asano, Takumi Nishiuchi, Seiichi Matsugo, Kenji Ishihara, Yu Kanesaki, Hirofumi Yoshikawa, Toshio Sakamoto
Journal of General and Applied Microbiology, 2018
Nicotinamide mononucleotide and related metabolites induce disease resistance against fungal phytopathogens in Arabidopsis and barley
Akihiro Miwa, Yuji Sawada, Daisuke Tamaoki, Masami Yokota Hirai, Makoto Kimura, Kazuhiro Sato, Takumi Nishiuchi
Scientific Reports, 2017
The RB-IL-6 axis controls self-renewal and endocrine therapy resistance by fine-tuning mitochondrial activity
S Kitajima, A Yoshida, S Kohno, F Li, S Suzuki, N Nagatani, Y Nishimoto, N Sasaki, H Muranaka, Y Wan, T C Thai, N Okahashi, F Matsuda, H Shimizu, T Nishiuchi, Y Suzuki, K Tominaga, N Gotoh, M Suzuki, M E Ewen, D A Barbie, O Hirose, T Tanaka, C Takahashi
Oncogene, 2017
Synthesis, localization and possible function of serine (or cysteine) peptidase inhibitor, clade B, member 6a (Serpinb6a) in mouse submandibular gland
Hiroki Nakata, Miyuki Yamamoto, Tewarat Kumchantuek, Kannika Adhapanyawanich, Takumi Nishiuchi, Shoichi Iseki
Cell and Tissue Research, 2017
l-Threonine and its analogue added to autoclaved solid medium suppress trichothecene production by Fusarium graminearum
Kazuyuki Maeda, Yuichi Nakajima, Yoshikazu Tanahashi, Yoshiyuki Kitou, Akihiro Miwa, Kyoko Kanamaru, Tetsuo Kobayashi, Takumi Nishiuchi, Makoto Kimura
Archives of Microbiology, 2017
New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice
Chie Naruse, Shinwa Shibata, Masaru Tamura, Takayuki Kawaguchi, Kanae Abe, Kazushi Sugihara, Tomoaki Kato, Takumi Nishiuchi, Shigeharu Wakana, Masahito Ikawa, Masahide Asano
FASEB Journal, 2017
Identification of a trichothecene production inhibitor by chemical array and library screening using trichodiene synthase as a target protein
Kazuyuki Maeda, Yuichi Nakajima, Takayuki Motoyama, Yasumitsu Kondoh, Tatsuro Kawamura, Kyoko Kanamaru, Shuichi Ohsato, Takumi Nishiuchi, Minoru Yoshida, Hiroyuki Osada, Tetsuo Kobayashi, Makoto Kimura
Pesticide Biochemistry and Physiology, 2017
The morphogenesis-related NDR kinase pathway of Colletotrichum orbiculare is required for translating plant surface signals into infection-related morphogenesis and pathogenesis
Sayo Kodama, Junya Ishizuka, Ito Miyashita, Takaaki Ishii, Takumi Nishiuchi, Hideto Miyoshi, Yasuyuki Kubo
Plos Pathogens, 2017
Identification of sperm equatorial segment protein 1 in the acrosome as the primary binding target of peanut agglutinin (PNA) in the mouse testis
Hiroki Nakata, Tomohiko Wakayama, Tomoya Asano, Takumi Nishiuchi, Shoichi Iseki
Histochemistry and Cell Biology, 2017
Dysfunction of Arabidopsis MACPF domain protein activates programmed cell death via tryptophan metabolism in MAMP-triggered immunity
Satoshi Fukunaga, Miho Sogame, Masaki Hata, Suthitar Singkaravanit‐Ogawa, Mariola Piślewska‐Bednarek, Mariko Onozawa‐Komori, Takumi Nishiuchi, Kei Hiruma, Hiromasa Saitoh, Ryohei Terauchi, Saeko Kitakura, Yoshihiro Inoue, Paweł Bednarek, Paul Schulze‐Lefert, Yoshitaka Takano
Plant Journal, 2017
MicroRNA-140 mediates RB tumor suppressor function to control stem cell-like activity through interleukin-6
Akiyo Yoshida, Shunsuke Kitajima, Fengkai Li, Chaoyang Cheng, Yujiro Takegami, Susumu Kohno, Yuan Song Wan, Naoyuki Hayashi, Hayato Muranaka, Yuuki Nishimoto, Naoko Nagatani, Takumi Nishiuchi, Tran C Thai, Sawako Suzuki, Shinji Nakao, Tomoaki Tanaka, Osamu Hirose, David A. Barbie, Chiaki Takahashi
Oncotarget, 2017
An in vitro system to characterize prostate cancer progression identified signaling required for self-renewal
Mohammed Salah, Yuuki Nishimoto, Susumu Kohno, Atsushi Kondoh, Shunsuke Kitajima, Hayato Muranaka, Takumi Nishiuchi, Ahmed Ibrahim, Akiyo Yoshida, Chiaki Takahashi
Molecular Carcinogenesis, 2016
An S-adenosyl methionine synthetase (SAMS) gene from Andropogon virginicus L. confers aluminum stress tolerance and facilitates epigenetic gene regulation in Arabidopsis thaliana
Bunichi Ezaki, Aiko Higashi, Norie Nanba, Takumi Nishiuchi
Frontiers in Plant Science, 2016
Hydroxylations of trichothecene rings in the biosynthesis of Fusarium trichothecenes: Evolution of alternative pathways in the nivalenol chemotype
Kazuyuki Maeda, Akira Tanaka, Ryosuke Sugiura, Hiroyuki Koshino, Takeshi Tokai, Masayuki Sato, Yuichi Nakajima, Yoshikazu Tanahashi, Kyoko Kanamaru, Tetsuo Kobayashi, Takumi Nishiuchi, Makoto Fujimura, Naoko Takahashi‐Ando, Makoto Kimura
Environmental Microbiology, 2016
Colletotrichum orbiculare WHI2, a yeast stress-response regulator homolog, controls the biotrophic stage of hemibiotrophic infection through TOR signaling
Ken Harata, Takumi Nishiuchi, Yasuyuki Kubo
Molecular Plant Microbe Interactions, 2016
Exosomal transfer of functional small RNAs mediates cancer-stroma communication in human endometrium
Yoshiko Maida, Masahiro Takakura, Takumi Nishiuchi, Tanihiro Yoshimoto, Satoru Kyo
Cancer Medicine, 2016
Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma
Eri Adachi, Katsuya Sakai, Takumi Nishiuchi, Ryu Imamura, Hiroki Sato, Kunio Matsumoto
Oncotarget, 2016
Re-examination of genetic and nutritional factors related to trichothecene biosynthesis in Fusarium graminearum
Yoshiyuki Kitou, Yuichi Nakajima, Kazuyuki Maeda, Qi Jin, Takumi Nishiuchi, Kyoko Kanamaru, Tetsuo Kobayashi, Makoto Kimura
Bioscience Biotechnology and Biochemistry, 2016
Characterization of the acivicin effects on trichothecene production by Fusarium graminearum species complex
Kazuyuki Maeda, Yuichi Nakajima, Yoshikazu Tanahashi, Takahiro Kosaki, Yoshiyuki Kitou, Kyoko Kanamaru, Tetsuo Kobayashi, Takumi Nishiuchi, Makoto Kimura
Journal of General and Applied Microbiology, 2016
Responses to intermittent exposure to green light during the dark period in Arabidopsis thaliana and possible involvement of cryptochrome 2
Masayuki Sato, Takumi Nishiuchi, Toshio Sakamoto
Plant Biotechnology, 2015
Effect of disrupting the trichothecene efflux pump encoded by FgTri12 in the nivalenol chemotype of Fusarium graminearum
Yuichi Nakajima, Norifumi Koseki, Ryosuke Sugiura, Naoki Tominaga, Kazuyuki Maeda, Takeshi Tokai, Masumi Izawa, Kyoko Kanamaru, Takashi Kamakura, Tetsuo Kobayashi, Takumi Nishiuchi, Minoru Yoshida, Makoto Kimura
Journal of General and Applied Microbiology, 2015
A marine phytoplankton Prymnesium parvum upregulates the component proteins of photosystem II under iron stress
M. M. Rahman, M. A. Rahman, T. Maki, T. Nishiuchi, T. Asano, H. Hasegawa
Photosynthetica, 2015
Morphology and gene expression profile of the submandibular gland of androgen-receptor-deficient mice
Kannika Adthapanyawanich, Tewarat Kumchantuek, Hiroki Nakata, Miyuki Yamamoto, Tomohiko Wakayama, Takumi Nishiuchi, Shoichi Iseki
Archives of Oral Biology, 2015
Corrigendum to "characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune" [Journal of Photochemistry and Photobiology B: Biology 142 (2015) 154-168]
Ehsan Nazifi, Naoki Wada, Tomoya Asano, Takumi Nishiuchi, Yoshiaki Iwamuro, Satoshi Chinaka, Seiichi Matsugo, Toshio Sakamoto
Journal of Photochemistry and Photobiology B Biology, 2015
Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune
Ehsan Nazifi, Naoki Wada, Tomoya Asano, Takumi Nishiuchi, Yoshiaki Iwamuro, Satoshi Chinaka, Seiichi Matsugo, Toshio Sakamoto
Journal of Photochemistry and Photobiology B Biology, 2015
Quantitative phosphoproteomic analysis using iTRAQ method
Tomoya Asano, Takumi Nishiuchi
Methods in Molecular Biology, 2014
Effects of hypergravity stimulus on global gene expression during reproductive growth in Arabidopsis
D. Tamaoki, I. Karahara, T. Nishiuchi, T. Wakasugi, K. Yamada, S. Kamisaka
Plant Biology, 2014
A marine phytoplankton (Prymnesium parvum) up-regulates ABC transporters and several other proteins to acclimatize with Fe-limitation
M. Mamunur Rahman, M. Azizur Rahman, T. Maki, T. Nishiuchi, T. Asano, H. Hasegawa
Chemosphere, 2014
Effects of acivicin on growth, mycotoxin production and virulence of phytopathogenic fungi
K. Maeda, Y. Nakajima, T. Motoyama, Y. Kitou, T. Kosaki, T. Saito, T. Nishiuchi, K. Kanamaru, H. Osada, T. Kobayashi, M. Kimura
Letters in Applied Microbiology, 2014
Visualization of neural activity in insect brains using a conserved immediate early gene, Hr38
Nozomi Fujita, Yuka Nagata, Takumi Nishiuchi, Makoto Sato, Masafumi Iwami, Taketoshi Kiya
Current Biology, 2013
The Secreted Antifungal Protein Thionin 2.4 in Arabidopsis thaliana Suppresses the Toxicity of a Fungal Fruit Body Lectin from Fusarium graminearum
Tomoya Asano, Akihiro Miwa, Kazuyuki Maeda, Makoto Kimura, Takumi Nishiuchi
Plos Pathogens, 2013
Thermophile-fermented compost as a possible scavenging feed additive toprevent peroxidation
Hirokuni Miyamoto, Eiji Shimada, Takashi Satoh, Ryusuke Tanaka, Kenshiro Oshima, Wataru Suda, Shinji Fukuda, Takumi Nishiuchi, Makiko Matsuura, Kenichi Mori, Hisashi Miyamoto, Hiroshi Ohno, Masahira Hattori, Hiroaki Kodama, Teruo Matsushita
Journal of Bioscience and Bioengineering, 2013
Quantification of metabolic activity of cultured plant cells by vital staining with fluorescein diacetate
Naoko Saruyama, Yurina Sakakura, Tomoya Asano, Takumi Nishiuchi, Hamako Sasamoto, Hiroaki Kodama
Analytical Biochemistry, 2013
Glycosylated porphyra-334 and palythine-threonine from the terrestrial cyanobacterium nostoc commune
Ehsan Nazifi, Naoki Wada, Minami Yamaba, Tomoya Asano, Takumi Nishiuchi, Seiichi Matsugo, Toshio Sakamoto
Marine Drugs, 2013
ATM mediates prb function to control dnmt1 protein stability and dna methylation
Awad Shamma, Misa Suzuki, Naoyuki Hayashi, Masahiko Kobayashi, Nobunari Sasaki, Takumi Nishiuchi, Yuichiro Doki, Takahiro Okamoto, Susumu Kohno, Hayato Muranaka, Shunsuke Kitajima, Ken-ichi Yamamoto, Chiaki Takahashi
Molecular and Cellular Biology, 2013
Zebrafish scales respond differently to in vitro dynamic and static acceleration: Analysis of interaction between osteoblasts and osteoclasts
Kei-ichiro Kitamura, Koh Takahira, Masato Inari, Yusuke Satoh, Kazuichi Hayakawa, Yoshiaki Tabuchi, Kazuhiro Ogai, Takumi Nishiuchi, Takashi Kondo, Yuko Mikuni-Takagaki, Wenxi Chen, Atsuhiko Hattori, Nobuo Suzuki
Comparative Biochemistry and Physiology A Molecular and Integrative Physiology, 2013
Proteomic analysis of S-nitrosylation induced by 1-methyl-4-phenylpyridinium (MPP+)
Akira T Komatsubara, Tomoya Asano, Hiroki Tsumoto, Kazuharu Shimizu, Takumi Nishiuchi, Masanori Yoshizumi, Kentaro Ozawa
Proteome Science, 2012
Impact of oral administration of compost extract on gene expression in the rat gastrointestinal tract
Takashi Satoh, Takumi Nishiuchi, Tomoaki Naito, Teruo Matsushita, Hiroaki Kodama, Hisashi Miyamoto, Hirokuni Miyamoto
Journal of Bioscience and Bioengineering, 2012
The defense response in Arabidopsis thaliana against Fusarium sporotrichioides
Tomoya Asano, Makoto Kimura, Takumi Nishiuchi
Proteome Science, 2012
Prostaglandin E2 increases both osteoblastic and osteoclastic activity in the scales and participates in calcium metabolism in goldfish
Katsunori Omori, Shigehito Wada, Yusuke Maruyama, Atsuhiko Hattori, Kei-Ichiro Kitamura, Yusuke Sato, Masayuki Nara, Hisayuki Funahashi, Koji Yachiguchi, Kazuichi Hayakawa, Masato Endo, Rei Kusakari, Sachiko Yano, Ajai K. Srivastav, Takashi Kusui, Sadakazu Ejiri, Wenxi Chen, Yoshiaki Tabuchi, Yukihiro Furusawa, Takashi Kondo, Yuichi Sasayama, Takumi Nishiuchi, Masaki Nakano, Tatsuya Sakamoto, Nobuo Suzuki
Zoological Science, 2012
Compensatory upregulation of myelin protein zero-like 2 expression in spermatogenic cells in cell adhesion molecule-1-deficient mice
Hiroki Nakata, Tomohiko Wakayama, Kannika Adthapanyawanich, Takumi Nishiuchi, Yoshinori Murakami, Yoshimi Takai, Shoichi Iseki
Acta Histochemica Et Cytochemica, 2012
Erratum: Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice (Journal of Clinical Investigation (2010) 120, 11, (3979-3995) DOI:10.1172/JCI42315)
Fei Wang, Yasuo Okamoto, Isao Inoki, Kazuaki Yoshioka, Wa Du, Xun Qi, Noriko Takuwa, Koichi Gonda, Yasuhiko Yamamoto, Ryunosuke Ohkawa, Takumi Nishiuchi, Naotoshi Sugimoto, Yutaka Yatomi, Kunitoshi Mitsumori, Masahide Asano, Makoto Kinoshita, Yoh Takuwa
Journal of Clinical Investigation, 2012
Proteomic analysis of susceptible rice plants expressing the whole plant-specific resistance against Magnaporthe oryzae: Involvement of a thaumatin-like protein
Hironori Koga, Koji Dohi, Takumi Nishiuchi, Tomoaki Kato, Hiroyuki Takahara, Masashi Mori, Setsuko Komatsu
Physiological and Molecular Plant Pathology, 2012
Fungal elicitor-induced retardation and its restoration of root growth in tobacco seedlings
Yoshio Kawaguchi, Takumi Nishiuchi, Hiraoki Kodama, Toshitsugu Nakano, Kazuma Nishimura, Katsuyoshi Shimamura, Kazuo Yamaguchi, Kazuyuki Kuchitsu, Hideaki Shinshi, Kaoru Suzuki
Plant Growth Regulation, 2012
Involvement of auxin dynamics in hypergravity-induced promotion of lignin-related gene expression in Arabidopsis inflorescence stems
D. Tamaoki, I. Karahara, T. Nishiuchi, T. Wakasugi, K. Yamada, S. Kamisaka
Journal of Experimental Botany, 2011
Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice
Kanae Abe, Chie Naruse, Tomoaki Kato, Takumi Nishiuchi, Mitinori Saitou, Masahide Asano
Biology of Reproduction, 2011
Caspase-1 protein induces apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)-mediated necrosis independently of its catalytic activity
Kou Motani, Hiroko Kushiyama, Ryu Imamura, Takeshi Kinoshita, Takumi Nishiuchi, Takashi Suda
Journal of Biological Chemistry, 2011
Arabidopsis ENHANCED DISEASE RESISTANCE 1 is required for pathogen-induced expression of plant defensins in nonhost resistance, and acts through interference of MYC2-mediated repressor function
Kei Hiruma, Takumi Nishiuchi, Tomoaki Kato, Paweł Bednarek, Tetsuro Okuno, Paul Schulze‐Lefert, Yoshitaka Takano
Plant Journal, 2011
Comparative anatomy of embryogenesis in three species of Podostemaceae and evolution of the loss of embryonic shoot and root meristems
Natsu Katayama, Masahiro Kato, Takumi Nishiuchi, Toshihiro Yamada
Evolution and Development, 2011
Gene expression analysis of wounding-induced root-to-shoot communication in Arabidopsis thaliana
SATOKO HASEGAWA, YUSUKE SOGABE, TOMOYA ASANO, TOMOYUKI NAKAGAWA, HARUNA NAKAMURA, HIROAKI KODAMA, HIROYUKI OHTA, KAZUO YAMAGUCHI, MARTIN J. MUELLER, TAKUMI NISHIUCHI
Plant Cell and Environment, 2011
Comparative Analysis of Phosphoprotein Expression Using 2D-DIGE
Tomoya Asano, Takumi Nishiuchi
Methods in Molecular Biology, 2011
Visualization of wounding-induced root-to-shoot communication in Arabidopsis
Yusuke Sogabe, Haruna Nakamura, Tomoyuki Nakagawa, Satoko Hasegawa, Tomoya Asano, Hiroyuki Ohta, Kazuo Yamaguchi, Martin J. Mueller, Hiroaki Kodama, Takumi Nishiuchi
Plant Signaling and Behavior, 2011
HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnaporthe oryzae
Shigeyuki Tanaka, Akari Ichikawa, Kaori Yamada, Gento Tsuji, Takumi Nishiuchi, Masashi Mori, Hironori Koga, Yoko Nishizawa, Richard O'Connell, Yasuyuki Kubo
BMC Plant Biology, 2010
Sphingosine-1-phosphate receptor-2 deficiency leads to inhibition of macrophage proinflammatory activities and atherosclerosis in apoE-deficient mice
Fei Wang, Yasuo Okamoto, Isao Inoki, Kazuaki Yoshioka, Wa Du, Xun Qi, Noriko Takuwa, Koichi Gonda, Yasuhiko Yamamoto, Ryunosuke Ohkawa, Takumi Nishiuchi, Naotoshi Sugimoto, Yutaka Yatomi, Kunitoshi Mitsumori, Masahide Asano, Makoto Kinoshita, Yoh Takuwa
Journal of Clinical Investigation, 2010
Transcriptome profiling in Arabidopsis inflorescence stems grown under hypergravity in terms of cell walls and plant hormones
D. Tamaoki, I. Karahara, T. Nishiuchi, S. De Oliveira, L. Schreiber, T. Wakasugi, K. Yamada, K. Yamaguchi, S. Kamisaka
Advances in Space Research, 2009
Mechanism and repertoire of ASC-mediated gene expression
Mizuho Hasegawa, Ryu Imamura, Kou Motani, Takumi Nishiuchi, Norihiko Matsumoto, Takeshi Kinoshita, Takashi Suda
Journal of Immunology, 2009
A screening system for inhibitors of trichothecene biosynthesis: Hydroxylation of trichodiene as a target
Naoko Takahashi-Ando, Noriyuki Ochiai, Takeshi Tokai, Shuichi Ohsato, Takumi Nishiuchi, Minoru Yoshida, Makoto Fujimura, Makoto Kimura
Biotechnology Letters, 2008
AtNFXL1, an Arabidopsis homologue of the human transcription factor NF-X1, functions as a negative regulator of the trichothecene phytotoxin-induced defense response
Tomoya Asano, Daisuke Masuda, Michiko Yasuda, Hideo Nakashita, Toshiaki Kudo, Makoto Kimura, Kazuo Yamaguchi, Takumi Nishiuchi
Plant Journal, 2008
The AtNFXL1 gene functions as a signaling component of the type A trichothecene-dependent response
Tomoya Asano, Michiko Yasuda, Hideo Nakashita, Makoto Kimura, Kazuo Yamaguchi, Takumi Nishiuchi
Plant Signaling and Behavior, 2008
Involvement of the osmosensor histidine kinase and osmotic stress-activated protein kinases in the regulation of secondary metabolism in Fusarium graminearum
Noriyuki Ochiai, Takeshi Tokai, Takumi Nishiuchi, Naoko Takahashi-Ando, Makoto Fujimura, Makoto Kimura
Biochemical and Biophysical Research Communications, 2007
High-level overexpression of the Arabidopsis HsfA2 gene confers not only increased themotolerance but also salt/osmotic stress tolerance and enhanced callus growth
D. Ogawa, K. Yamaguchi, T. Nishiuchi
Journal of Experimental Botany, 2007
Comprehensive analysis of early response genes to two different microbial elicitors in tobacco cells
Kaoru Suzuki, Akira Yano, Takumi Nishiuchi, Toshitsugu Nakano, Hiroaki Kodama, Kazuo Yamaguchi, Hideaki Shinshi
Plant Science, 2007
Phytotoxic effects of trichothecenes on the growth and morphology of Arabidopsis thaliana
D. Masuda, M. Ishida, K. Yamaguchi, I. Yamaguchi, M. Kimura, T. Nishiuchi
Journal of Experimental Botany, 2007
Transgenic rice plants expressing trichothecene 3-O-acetyltransferase show resistance to the Fusarium phytotoxin deoxynivalenol
Shuichi Ohsato, Tetsuko Ochiai-Fukuda, Takumi Nishiuchi, Naoko Takahashi-Ando, Shinzo Koizumi, Hiroshi Hamamoto, Toshiaki Kudo, Isamu Yamaguchi, Makoto Kimura
Plant Cell Reports, 2007
The chloroplast genome from a lycophyte (microphyllophyte), Selaginella uncinata, has a unique inversion, transpositions and many gene losses
Sumika Tsuji, Kunihiko Ueda, Tomoaki Nishiyama, Mitsuyasu Hasebe, Sumi Yoshikawa, Akihiko Konagaya, Takumi Nishiuchi, Kazuo Yamaguchi
Journal of Plant Research, 2007
Molecular biology and biotechnology for reduction of Fusarium mycotoxin contamination
Makoto Kimura, Naoko Takahashi-Ando, Takumi Nishiuchi, Shuichi Ohsato, Takeshi Tokai, Noriyuki Ochiai, Makoto Fujimura, Toshiaki Kudo, Hiroshi Hamamoto, Isamu Yamaguchi
Pesticide Biochemistry and Physiology, 2006
Studies on transcriptional regulation of endogenous genes by ERF2 transcription factor in tobacco cells
Toshitsugu Nakano, Takumi Nishiuchi, Kaoru Suzuki, Tatsuhito Fujimura, Hideaki Shinshi
Plant and Cell Physiology, 2006
Fusarium phytotoxin trichothecenes have an elicitor-like activity in Arabidopsis thaliana, but the activity differed significantly among their molecular species
Takumi Nishiuchi, Daisuke Masuda, Hideo Nakashita, Kazuya Ichimura, Kazuo Shinozaki, Shigeo Yoshida, Makoto Kimura, Isamu Yamaguchi, Kazuo Yamaguchi
Molecular Plant Microbe Interactions, 2006
Elicitor-induced down-regulation of cell cycle-related genes in tobacco cells
KAORU SUZUKI, TAKUMI NISHIUCHI, YUKO NAKAYAMA, MASAKI ITO, HIDEAKI SHINSHI
Plant Cell and Environment, 2006
Polyethylene glycol-mediated enhancement of the hybridization rate on cDNA microarrays
Mamiko Shimanuki, Katsuyoshi Shimamura, Sayaka Hirai, Takumi Nishiuchi, Kaoru Suzuki, Hiroaki Kodama
Analytical Biochemistry, 2005
Rapid and transient activation of transcription of the ERF3 gene by wounding in tobacco leaves: Possible involvement of NtWRKYs and autorepression
Takumi Nishiuchi, Hideaki Shinshi, Kaoru Suzuki
Journal of Biological Chemistry, 2004
Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins
Arisa HIGA, Makoto KIMURA, Kouhei MIMORI, Tetsuko OCHIAI-FUKUDA, Takeshi TOKAI, Naoko TAKAHASHI-ANDO, Takumi NISHIUCHI, Tomoko IGAWA, Makoto FUJIMURA, Hiroshi HAMAMOTO, Ron USAMI, Isamu YAMAGUCHI
Bioscience Biotechnology and Biochemistry, 2003
Wounding activates immediate early transcription of genes for ERFs in tobacco plants
Takumi Nishiuchi, Kaoru Suzuki, Sakihito Kitajima, Fumihiko Sato, Hideaki Shinshi
Plant Molecular Biology, 2002
Possible involvement of protein phosphorylation in the wound-responsive expression of Arabidopsis plastid ω-3 fatty acid desaturase gene
Hiroaki Kodama, Takumi Nishiuchi, Shigemi Seo, Yuko Ohashi, Koh Iba
Plant Science, 2000
Wound-induced expression of the FAD7 gene is mediated by different regulatory domains of its promoter in leaves/stems and roots
Takumi Nishiuchi, Hiroaki Kodama, Shuichi Yanagisawa, Koh Iba
Plant Physiology, 1999
Roles of plastid ω-3 fatty acid desaturases in defense response of higher plants
Takumi Nishiuchi, Koh Iba
Journal of Plant Research, 1998
Wounding changes the spatial expression pattern of the Arabidopsis plastid ω-3 fatty acid desaturase gene (FAD7) through different signal transduction pathways
T Nishiuchi, T Hamada, H Kodama, K Iba
Plant Cell, 1997
cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco
T. Hamada, T. Nishiuchi, H. Kodama, M. Nishimura, K. Iba
Plant and Cell Physiology, 1996
Tissue-specific and light-responsive regulation of the promoter region of the Arabidopsis thaliana chloroplast ω-3 fatty acid desaturase gene (FAD7)
Takumi Nishiuchi, Takiko Nakamura, Tsuyoshi Abe, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba
Plant Molecular Biology, 1995
Fatty acid desaturation during chilling acclimation is one of the factors involved in conferring low-temperature tolerance to young tobacco leaves
H. Kodama, G. Horiguchi, T. Nishiuchi, M. Nishimura, K. Iba
Plant Physiology, 1995
Genomic nucleotide sequence of a gene encoding a microsomal ω-3 fatty acid desaturase from Arabidopsis thaliana
T. Nishiuchi, M. Nishimura, V. Arondel, K. Iba
Plant Physiology, 1994
A gene encoding a chloroplast ω-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the fad7 mutant of Arabidopsis thaliana
K Iba, S Gibson, T Nishiuchi, T Fuse, M Nishimura, V Arondel, S Hugly, C Somerville
Journal of Biological Chemistry, 1993

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