SANGARAJU RAJENDRA
@rmit.edu.au
ICMR-Research Associate
ICMR National Institute of Nutrition, INDIA and RMIT UNIVERSITY MELBOURNE Australia
EDUCATION
B. Pharm, M. Pharm ( Pharmacology), Ph.D. Biological Sciences/ Pharmaceutical Sciences/ Pharmacology//Inflammo Pharmacology
RESEARCH, TEACHING, or OTHER INTERESTS
General Pharmacology, Toxicology and Pharmaceutics, Pharmacy, General Biochemistry, Genetics and Molecular Biology, Food Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Priyanka Raju Chougule, Rajendra Sangaraju, Pradeep B. Patil, S. S. Y. H. Qadri, Virendra V. Panpatil, Sudip Ghosh, Sathish Kumar Mungamuri, Manjula Bhanoori, and Sukesh Narayan Sinha
Springer Science and Business Media LLC
Sukesh Narayan Sinha, Ramakrishna Ungarala, Dileshwar Kumar, Rajendra Sangaraju, and Sarita Kumar
Public Library of Science (PLoS)
Several methods have been reported to estimate Acetylcholinesterase (AChE) enzyme activity in blood samples. The Ellman assay is the most important among all but with several shortcomings, and there is a need to develop a method which is accurate, sensitive and quick for analyzing AChE. Therefore, we have developed an assay utilizing RP-HPLC with UV detection for the determination of AChE activity. This method measured the conversion of 1-naphthol acetate to 1-naphthol to estimate AChE activity in blood samples. Performance was judged on the basis of reproducibility, sensitivity, accuracy, and the ability to screen enzyme activity within 20minutes. A series of experiments were performed, varying the concentration of blood and substrate, with optimal sensitivity using 50 μM substrate and 10μL blood. The validation parameters such as linearity (R2 of ≥ 0.9842 for 1-naphthol and ≥ 0.9897 for 1-naphthol acetate), precision (94.21–96.41%), accuracy (85.2%–99.6% and 82.6%–99.3% for 1-naphthol and 1-naphthol acetate respectively), and robustness were validated according to International Conference on Harmonization (ICH) guidelines. Blood samples were collected from healthy people, farmers exposed to spraying of pesticides, and suicidal patients who ingested pesticides and were hospitalized and were analyzed by the developed method. The AChE level was approximately 21 units/mL compared to 24units/mL in controls, whereas suicidal patients showed the least AChE levels of 1 unit/mL. The employment of this method is recommended for estimating AChE level on various matrices.
Sukesh Narayan Sinha, Rajesh Kumar Kumpati, Pandu Naik Ramavath, Rajendra Sangaraju, Balaji Gouda, and Priyanka Chougule
Springer Science and Business Media LLC
AbstractThe aim of this study was to investigate the sociodemographic characteristics of patients based on the poison chosen and different types of organophosphorus compounds. The data were collected to explore the sociodemographic characteristics of organophosphate (OP)-poisoned patients based on the source, site, and route of poisoning, education level, occupational status, and the purpose of poisoning. Furthermore, we estimated the serotonin and dopamine levels in the plasma samples of patients, and survival plots were also described. During the study of OP pesticide poisoning in 116 human subjects and 5 healthy volunteers, we observed, based on the survival plot, that75.9% of the patients were discharged, and the remaining patients died (24.1% of the patients) due to respiratory failure followed by cardiac arrest. Our findings suggest that the serotonin levels significantly (p < 0.01 and p < 0.001) decreased from 12 to 36 h, whereas the dopamine levels slightly increased from 12 to 36 h in the group with OP poisoning compared to the control group. Based on these findings, this study may aid in deciphering the precise mechanism by which pesticides cause behavioural changes that influence serotonin and dopamine levels in OP-poisoned patients. The purpose of this work was to serve as a small reminder of the risk to public health associated with organophosphate pesticides.
Rajendra Sangaraju, Sateesh Alavala, Nasiruddin Nalban, Mahesh Kumar Jerald, and Ramakrishna Sistla
Elsevier BV
Sateesh Alavala, Nasiruddin Nalban, Rajendra Sangaraju, Madhusudana Kuncha, Mahesh Kumar Jerald, Eswar Kumar Kilari, and Ramakrishna Sistla
Springer Science and Business Media LLC
Nasiruddin Nalban, Rajendra Sangaraju, Sateesh Alavala, Salma Mukhtar Mir, Mahesh Kumar Jerald, and Ramakrishna Sistla
Springer Science and Business Media LLC
Chandrashekhar Madasu, Shailaja Karri, Rajendra Sangaraju, Ramakrishna Sistla, and Mallavadhani Venkata Uppuluri
Elsevier BV
Rajendra Sangaraju, Nasiruddin Nalban, Sateesh Alavala, Vinoth Rajendran, Mahesh Kumar Jerald, and Ramakrishna Sistla
Springer Science and Business Media LLC
Sateesh Alavala, Rajendra Sangaraju, Nasiruddin Nalban, Bidya Dhar Sahu, Mahesh Kumar Jerald, Eswar Kumar Kilari, and Ramakrishna Sistla
Elsevier BV
Nasiruddin Nalban, Sateesh Alavala, Rajendra Sangaraju, Salma Mukhtar Mir, and Ramakrishna Sistla
Springer Singapore
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
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Industry, Institute, or Organisation Collaboration
CSIR INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY
INDIAN COUNCIL OF MEDICAL RESEARCH
NATIONAL INSTITUTE OF NUTRITION
JNTU ANANTAPUR
RMIT UNIVERSITY MELBOURNE
INDUSTRY EXPERIENCE
Prof. Brien Holden Eye Research Foundation
LVPEI HOSPITAL
DRUG TESTING LABORATORY at CSIR IICT