In Vitro Comparative Analysis of Antioxidant Activities in Ethanolic Extracts of Centella Asiatica Linn., Ocimum Basilicum, Ocimum Sanctum, and Camellia Sinensis Nazifa Javaid, Meghanand T Nayak, Anjali Nayak, Rezhat Abass, Mohd. Zanul Abedeen, Milind Sharad Pande Journal of Craniomaxillofacial Research, 2026 Introduction: To comparatively evaluate the antioxidant potential of ethanolic extracts of Centella asiatica Linn. (CA), Ocimum basilicum Linn. (OB), Ocimum sanctum (OS), and Camellia sinensis (CS) using multiple in vitro assays.Materials and Methods: Ethanolic extracts of CA, OB, OS, and CS were prepared and subjected to in vitro antioxidant analysis, including total antioxidant capacity, DPPH radical scavenging, nitric oxide radical scavenging, reducing power, and superoxide anion radical scavenging assays using UV spectrophotometry. Antioxidant activities were recorded at concentrations up to 1000 µg/ml. All assays were performed across a concentration range of 10–1000 µg/ml using serial dilutions.Results: Total antioxidant activity (109.40 ± 0.15 % inhibition), DPPH scavenging (107.88 ± 9.32 % inhibition), nitric oxide scavenging (98.27 ± 9.48 % inhibition), reducing power (absorbance: 93.12 ± 9.12 at 700 nm), and superoxide scavenging (88.93 ± 8.14 % inhibition). The differences between groups were statistically significant (p < 0.001).Conclusion: CA demonstrated superior antioxidant activity compared to OB, OS, and CS. Its high antioxidant potential suggests its promise as a natural therapeutic agent for preventing oxidative stress–related diseases.
Recent Trends on Plants and Agricultural Products as Nutritional Source in Treating Diabetes Suryakant Verma, Milind Sharad Pande Recent Advances in Food Nutrition and Agriculture, 2026 A metabolic disease that requires insulin and is marked by consistently high blood sugar levels is known as diabetes mellitus. Many healthcare systems throughout the world have long relied on medicinal herbs as a means of addressing diabetes and its complications. Traditional medicine derived from plant extracts has several advantages over contemporary pharmaceuticals, including lower costs, greater clinical efficacy, and fewer side effects. Primarily, the condition has been managed by a range of synthetic medications that improve the altered glycemic state in individuals with diabetes. Synthetic medications work well, but along with their benefits, they come with noticeable adverse effects. Due to the lack of knowledge regarding their chemical composition, preparation method, active bio-actives, potential side effects, and the optimal way to administer them, medicinal plants have not been fully utilised as acceptable drugs in the treatment of diabetes, despite their long history of use as primary health care. Because of a lack of sufficient data on the parameters described earlier, most medicinal plants that show promise as anti-diabetic agents do not make it to the clinical trial phase. Medicinal plants that have been studied in humans with diabetes and shown promise as a treatment for the disease, either alone or in conjunction with other plants, are summarised in this review. Pharmacologically active phytomolecules with an antidiabetic action that are derived from medicinal plants were the primary topic of this review article. Its goal was to discuss their importance in diabetes management and therapy. These all-natural substances have the potential to be successful and alternative diabetes treatments, as well as a new method of approaching the disease.
Formulation, Optimization and Evaluation of Wound Healing activity of Lawsone-loaded Scaffold based gel for Topical Delivery Lovely Chaurasia, Milind Sharad Pande, Mojahidul Islam International Journal of Drug Delivery Technology, 2026 The skin acts as a vital protective barrier and plays a crucial role in maintaining homeostasis, sensory perception, and defense against external pathogens. Disruption of skin integrity results in wound formation, which may be acute or chronic depending on the healing duration. Chronic wounds, particularly those associated with conditions such as diabetes and vascular disorders, present significant therapeutic challenges. In recent years, hydrogels have emerged as promising woundhealing biomaterials due to their structural similarity to the extracellular matrix, high water retention, flexibility, and ability to deliver therapeutic agents. In the present study, chitosan and polyvinyl alcohol (PVA)–based hydrogel scaffolds loaded with 2-hydroxy-1,4-naphthoquinone were developed using a freeze-drying technique. Various scaffold formulations were prepared by altering chitosan:PVA ratios and were evaluated for particle size, entrapment efficiency, in-vitro drug release, rheological behavior, and physicochemical properties. Optimization was carried out using a Central Composite Design (CCD) to study the influence of polymer concentrations on particle size and entrapment efficiency. Among the formulations, F4 (chitosan:PVA, 2:1) exhibited the highest entrapment efficiency (92.45 ± 0.15%) with an optimal particle size (148 ± 0.15 nm). The optimized formulation demonstrated sustained drug release over 12 hours, suitable pH for topical application, good spreadability, homogeneity, and pseudoplastic rheological behavior. In vivo wound-healing and histopathological studies revealed enhanced re-epithelialization, collagen deposition, reduced inflammation, and improved granulation tissue formation in drug-loaded hydrogel–treated groups compared to controls. Overall, the developed chitosan/PVA hydrogel scaffold shows significant potential as an effective wound-healing system, offering sustained drug delivery, improved healing response, and favorable biocompatibility for topical applications.
A clinical and microbiological study to assess the efficacy of Acmella oleracea and Acacia catechu herbs as local drug delivery in treatment of chronic generalized periodontitis patients Kanika Verma, Deepa Dhruvakumar, Milind Pande Journal of Indian Society of Periodontology, 2022 Aim: A randomized controlled clinical trial was conducted to evaluate the effect of local drug delivery (LDD) of Acmella oleracea (Akarkara) and Acacia catechu (kattha) gels as an adjunct to scaling and root planing (SRP) in generalized chronic periodontitis patients. Materials and Methods: Thirty patients with ninety sites aged 25–65 years with generalized chronic periodontitis having pocket probing depth (PPD) of more than or equal to 5 mm on the basis of inclusion and exclusion criteria were selected. They were treated according to split-mouth design protocol. The placement of gel containing A. oleracea (1%) (Group 1) and A. catechu (1%) (Group 2) as LDD was placed at the two test sites, respectively, and only SRP was done in the control group (Group 3). Periodontal dressing was applied to all the three sites after procedure, and patients were given oral care guidelines. Evaluation of clinical parameters gingival index (GI), plaque index (PI), gingival bleeding index (GBI), PPD, clinical attachment level (CAL), and relative attachment level (RAL) was done at baseline, 1, 3, and 6 months, and microbiological parameter was assessed at baseline and 6 months. N-benzoyl-DL-arginine-2-napthylamide (BANA) test was used for the microbiological analysis. Results: Clinical parameters from baseline to 6 months at different time intervals demonstrated a significant reduction in mean GI, PI, GBI, and PPD and gain in CAL and RAL over a period of 6 months, and results were statistically significant in all the three treatment groups. However, on intergroup comparison, clinically significant difference was observed, but statistically, results were significant at few intervals. The microbiological analysis revealed a statistically nonsignificant reduction at the end of 6 months on intra- and intergroup comparison. Conclusion: The study concluded that the use of A. catechu gel and A. oleracea gel clinically showed improvement in clinical parameters when combined with SRP, attributing to better anti-inflammatory and healing properties.
Neuropharmacological screening of fronds of Adiantum capillus veneris linn Der Pharmacia Lettre, 2014
Immunomodulatory activity of gymnema sylvestre leaves Internet Journal of Pharmacology, 2010
Standardization of Arjunarishta formulation by TLC method International Journal of Pharmaceutical Sciences Review and Research, 2010
Preliminary Pharmacognostic evaluations and Phytochemical studies on roots of Mimosa pudica (Lajvanti) International Journal of Pharmaceutical Sciences Review and Research, 2010
Investigation of aphrodisiac potential of Blepharis edulis Linn. (Utangan) claimed by tribals of Malwa Region of Madhya Pradesh International Journal of Chemtech Research, 2009
Effect of ethanolic extract of Ocimum gratissimum (ram tulsi) on sexual behaviour in male mice International Journal of Pharmtech Research, 2009
