Aleksandra Kurzyńska
@uwm.edu.pl
Department of Animal Anathomy and Physiology, Faculty of Biology and Biotechnology
University of Warmia and Mazury
Scopus Publications
Scopus Publications
Karol Mierzejewski, Aleksandra Kurzyńska, Monika Golubska, Jarosław Całka, Ismena Gałęcka, Mariusz Szabelski, Łukasz Paukszto, Aneta Andronowska, and Iwona Bogacka
Elsevier BV
Karol Mierzejewski, Zuzanna Gerwel, Aleksandra Kurzyńska, Monika Golubska, and Iwona Bogacka
Elsevier BV
Karol Mierzejewski, Aleksandra Kurzyńska, Zuzanna Gerwel, Monika Golubska, Robert Stryiński, and Iwona Bogacka
MDPI AG
Inflammation in the female reproductive system causes serious health problems including infertility. The aim of this study was to determine the in vitro effects of peroxisome proliferator-activated receptor-beta/delta (PPARβ/δ) ligands on the transcriptomic profile of the lipopolysaccharide (LPS)-stimulated pig corpus luteum (CL) in the mid-luteal phase of the estrous cycle using RNA-seq technology. The CL slices were incubated in the presence of LPS or in combination with LPS and the PPARβ/δ agonist—GW0724 (1 μmol/L or 10 μmol/L) or the antagonist—GSK3787 (25 μmol/L). We identified 117 differentially expressed genes after treatment with LPS; 102 and 97 differentially expressed genes after treatment, respectively, with the PPARβ/δ agonist at a concentration of 1 μmol/L or 10 μmol/L, as well as 88 after the treatment with the PPARβ/δ antagonist. In addition, biochemical analyses of oxidative status were performed (total antioxidant capacity and activity of peroxidase, catalase, superoxide dismutase, and glutathione S-transferase). This study revealed that PPARβ/δ agonists regulate genes involved in the inflammatory response in a dose-dependent manner. The results indicate that the lower dose of GW0724 showed an anti-inflammatory character, while the higher dose seems to be pro-inflammatory. We propose that GW0724 should be considered for further research to alleviate chronic inflammation (at the lower dose) or to support the natural immune response against pathogens (at the higher dose) in the inflamed corpus luteum.
Karol Mierzejewski, Łukasz Paukszto, Aleksandra Kurzyńska, Zuzanna Kunicka, Jan P. Jastrzębski, Karol G. Makowczenko, Monika Golubska, and Iwona Bogacka
Springer Science and Business Media LLC
AbstractInflammation is a biological response of the immune system, which can be triggered by many factors, including pathogens. These factors may induce acute or chronic inflammation in various organs, including the reproductive system, leading to tissue damage or disease. In this study, the RNA-Seq technique was used to determine the in vitro effects of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the expression of genes and long non-coding RNA, and alternative splicing events (ASEs) in LPS-induced inflammation of the porcine endometrium during the follicular phase of the estrous cycle. Endometrial slices were incubated in the presence of LPS and PPARγ agonists (PGJ2 or pioglitazone) and a PPARγ antagonist (T0070907). We identified 169, 200, 599 and 557 differentially expressed genes after LPS, PGJ2, pioglitazone or T0070907 treatment, respectively. Moreover, changes in differentially expressed long non-coding RNA and differential alternative splicing events were described after the treatments. The study revealed that PPARγ ligands influence the LPS-triggered expression of genes controlling the DNA damage response (GADD45β, CDK1, CCNA1, CCNG1, ATM). Pioglitazone treatment exerted a considerable effect on the expression of genes regulating the DNA damage response.
Karol Mierzejewski, Aleksandra Kurzyńska, Zuzanna Kunicka, Anna Klepacka, Monika Golubska, and Iwona Bogacka
Elsevier BV
Zuzanna Kunicka, Karol Mierzejewski, Aleksandra Kurzyńska, Robert Stryiński, Jesús Mateos, Mónica Carrera, Monika Golubska, and Iwona Bogacka
CSIRO Publishing
Context The corpus luteum (CL) is an endocrine gland in the ovary of mature females during the oestrous cycle and pregnancy. There is evidence of a relationship between the secretory function of the CL and PPARs. Aims In this study, we investigated the changes in the proteome of the CL in relation to the phase of the oestrous cycle and the impact of PPARγ ligands on the proteomic profile of the CL during the mid- and late-luteal phase of the oestrous cycle. Methods The porcine CL explants were incubated in vitro for 6 h in the presence of PPARγ ligands (agonist pioglitazone, antagonist T0070907) or without ligands. Global proteomic analysis was performed using the TMT-based LC-MS/MS method. Key results The obtained results showed the disparity in proteomic profile of the untreated CL – different abundance of 23 and 28 proteins for the mid- and late-luteal phase, respectively. Moreover, seven proteins were differentially regulated in the CL tissue treated with PPARγ ligands. In the mid-luteal phase, one protein, CAND1, was downregulated after treatment with T0070907. In the late-luteal phase, the proteins SPTAN1, GOLGB1, TP53BP1, MATR3, RRBP1 and SRRT were upregulated by pioglitazone. Conclusions Comparative proteomic analysis revealed that certain proteins constitute a specific proteomic signature for each examined phase. Moreover, the study showed that the effect of PPARγ ligands on the CL proteome was rather limited. Implications The results provide a broader insight into the processes that may be responsible for the structural luteolysis of the porcine CL, in addition to apoptosis and autophagy.
Aleksandra Kurzyńska, Zuzanna Kunicka, Karol Mierzejewski, Monika Golubska, and Iwona Bogacka
Elsevier BV
Karol Mierzejewski, Łukasz Paukszto, Aleksandra Kurzyńska, Zuzanna Kunicka, Jan Paweł Jastrzębski, and Iwona Bogacka
Oxford University Press (OUP)
Abstract Female fertility depends greatly on the capacity of the uterus to recognize and eliminate microbial infections, a major reason of inflammation in the endometrium in many species. This study aimed to determine the in vitro effect of peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the transcriptome genes expression and alternative splicing in the porcine endometrium in the mid-luteal phase of the estrous cycle during LPS-stimulated inflammation using RNA-seq technology. The endometrial slices were incubated in vitro in the presence of LPS and PPARγ agonists—PGJ2 or pioglitazone and antagonist—T0070907. We identified 222, 3, 4, and 62 differentially expressed genes after LPS, PGJ2, pioglitazone, or T0070907 treatment, respectively. In addition, we detected differentially alternative spliced events: after treatment with LPS-78, PGJ2-60, pioglitazone-52, or T0070907-134. These results should become a basis for further studies explaining the mechanism of PPARγ action in the reproductive system in pigs.
Zuzanna Kunicka, Aleksandra Kurzyńska, Anna Szydłowska, and Iwona Bogacka
Wiley
AbstractProblemCytokines are immune response mediators that play an important role in the regulation of reproductive functions. An association between cytokines and peroxisome proliferator receptors (PPARs) has been reported in various tissues, including the endometrium. The present study aimed to evaluate the impact of PPARα ligands on the expression of nuclear factor kappa B (NF‐κB) and cytokines (interleukin [IL]‐1β, IL‐4, IL‐6, IL‐8, IL‐10, and LIF) in the porcine endometrium in different reproductive stages.Methods of studyEndometrial slices were collected from gilts on days 10‐12 or 14‐16 of the estrous cycle and pregnancy. Endometrial tissue explants were incubated in vitro in the presence or absence of PPARα agonist WY‐14643 and antagonist MK886. Expression of mRNA and protein for NF‐ĸB and selected cytokines was evaluated by real‐time PCR and immunoblot.ResultsPPARα agonist WY‐14643 decreased the mRNA expression of NF‐κB in most of the analyzed stages (excluding days 10‐12 of the estrous cycle), but increased the expression of NF‐κB protein (excluding days 14‐16 of pregnancy). The WY‐14643 increased expression of IL‐1β and IL‐6 proteins, and the mRNA expression of IL‐8 and LIF, decreased IL‐4 expression, and did not affect the mRNA and protein expression of IL‐10.ConclusionThe obtained results demonstrate that PPARα is involved in the regulation of NF‐κB and cytokine expression in the porcine endometrium. PPARα ligands exert a varied influence on immune system components, which could be attributed to differences in the receptivity of porcine endometrial tissue during the reproductive cycle.
Zuzanna Kunicka, Aleksandra Kurzyńska, Anna Szydłowska, Beata Kaczyńska, and Iwona Bogacka
Elsevier BV
Zuzanna Kunicka, Aleksandra Kurzynska, Anna Szydlowska, Karol Mierzejewski, and Iwona Bogacka
Wiley
ProblemCytokines, mediators of the immune response, are involved in the regulation of female reproductive processes during the estrous cycle and pregnancy. The present study aimed to investigate the effect of selected peroxisome proliferator‐activated receptor gamma (PPARγ) ligands on the expression of nuclear factor kappa B (NF‐κB) and selected cytokines, such as interleukin (IL)‐1β, ‐4, ‐6, ‐8, ‐10, and the leukemia inhibitory factor, in the porcine endometrium on days 10‐12 and 14‐16 of the estrous cycle (mid‐ and late luteal phase, respectively) or pregnancy (maternal recognition of pregnancy and beginning of implantation, respectively).Method of studyEndometrial slices were incubated in vitro in the presence of PPARγ agonists, 15‐deoxy‐Δ12, 14‐prostaglandin J2 or rosiglitazone, and PPARγ antagonist T0070907. mRNA and protein levels in tissues were determined by real‐time PCR and Western blot.ResultsOn days 10‐12 of the estrous cycle and days 14‐16 of pregnancy, PPARγ ligands enhanced the expression of NF‐κB, mRNA cytokines, and/or proteins. During the late luteal phase of the estrous cycle (days 14‐16) and maternal recognition of pregnancy (days 10‐12), PPARγ ligands inhibited the expression of NF‐κB, and they differentially affected the expression of mRNA and proteins of cytokines.ConclusionOur results indicate that PPARγ is engaged in the endometrial synthesis of NF‐κB and selected cytokines in pigs. The influence of PPARγ ligands on the tested components of the immune system varied subject to the physiological status of females, and it could be associated with differences in endometrial receptivity.
Katarzyna Chojnowska, Joanna Czerwinska, Tadeusz Kaminski, Barbara Kaminska, Aleksandra Kurzynska, and Iwona Bogacka
Oxford University Press (OUP)
Abstract The European beaver (Castor fiber L.) is the largest free-living rodent in Eurasia. The present work aimed to determine sex- and season-related changes in leptin receptor (Ob-R) expression in the hypothalamic–pituitary–gonadal/adrenal axes and uterus of beavers during breeding- (April), post-breeding- (July), and pre-breeding- (November) periods. The expression of Ob-R gene and protein was found in all analyzed tissues. The expression of Ob-R mRNA remained constant in the hypothalamus of both sexes during the analyzed stages. Sex- and season-related changes were found in the pituitary gland; the greatest level was observed in July in both sexes. The same expression pattern was noted in the testis, whereas in the ovary a lack of seasonal changes was found. In uterine tissues, the greatest expression occurred in November. The impact of season was also demonstrated in the adrenal cortex. In females, a higher Ob-R transcript level was noted in April, while in males, an increased mRNA abundance was noted in November than July. Our study suggests that in the beaver, leptin acting via the Ob-R can be an important endocrine factor engaged in the regulation of reproductive functions and stress response.
Iwona Bogacka, Łukasz Paukszto, Jan P. Jastrzębski, Joanna Czerwińska, Katarzyna Chojnowska, Barbara Kamińska, Aleksandra Kurzyńska, Nina Smolińska, Zygmunt Giżejewski, and Tadeusz Kamiński
Public Library of Science (PLoS)
The European beaver (Castor fiber L.) is an important free-living rodent that inhabits Eurasian temperate forests. Beavers are often referred to as ecosystem engineers because they create or change existing habitats, enhance biodiversity and prepare the environment for diverse plant and animal species. Beavers are protected in most European Union countries, but their genomic background remains unknown. In this study, gene expression patterns in beaver testes and the variations in genetic expression in breeding and non-breeding seasons were determined by high-throughput transcriptome sequencing. Paired-end sequencing in the Illumina HiSeq 2000 sequencer produced a total of 373.06 million of high-quality reads. De novo assembly of contigs yielded 130,741 unigenes with an average length of 1,369.3 nt, N50 value of 1,734, and average GC content of 46.51%. A comprehensive analysis of the testicular transcriptome revealed more than 26,000 highly expressed unigenes which exhibited the highest homology with Rattus norvegicus and Ictidomys tridecemlineatus genomes. More than 8,000 highly expressed genes were found to be involved in fundamental biological processes, cellular components or molecular pathways. The study also revealed 42 genes whose regulation differed between breeding and non-breeding seasons. During the non-breeding period, the expression of 37 genes was up-regulated, and the expression of 5 genes was down-regulated relative to the breeding season. The identified genes encode molecules which are involved in signaling transduction, DNA repair, stress responses, inflammatory processes, metabolism and steroidogenesis. Our results pave the way for further research into season-dependent variations in beaver testes.
Katarzyna Chojnowska, Joanna Czerwinska, Tadeusz Kaminski, Barbara Kaminska, Aleksandra Kurzynska, and Iwona Bogacka
Elsevier BV
A. Kurzynska, K. Chojnowska, M. Bogacki, and I. Bogacka
Elsevier BV
A. Kurzyńska, M. Bogacki, K. Chojnowska, and I. Bogacka
Czech Academy of Agricultural Sciences
In the present study, we investigated the effect of PPAR ligands on progesterone (P4) and 17β-estradiol (E2) secretion, as well as 3β-hydroxysteroid dehydrogenase/Δ(5)-Δ(4) isomerase (3β-HSD) mRNA expression, in porcine endometrial slices collected on days 10–12 and 14–16 of the estrous cycle or early pregnancy. The explants were incubated in vitro for 6 h in the presence of PPARα ligands – WY-14643 (agonist) and MK 886 (antagonist); PPARβ ligands – L-165041 (agonist) and GW 9662 (antagonist); PPARγ ligands – 15d-prostaglandin J2 and rosiglitazone (agonists) and T0070907 (antagonist). During the estrous cycle, all PPAR ligands inhibited P4 secretion during the mid-luteal phase (days 10–12). During early pregnancy, a stimulatory effect of PPARα agonist was observed during maternal recognition of pregnancy (days 10–12), while an inhibitory effect was observed at the beginning of implantation (days 14–16). PPAR ligands inhibited the expression of 3β-HSD mRNA on days 14–16 of the estrous cycle (β and γ isoforms) or pregnancy (α, β, γ isoforms) but did not affect gene expression on days 10–12 of the estrous cycle or early pregnancy. An inhibitory effect of PPARα, PPARγ, and PPARβ on E2 secretion was observed during maternal recognition of pregnancy, but a stimulatory effect was observed during mid(γ isoform) or late-luteal (β isoform) phases of the estrous cycle. Our study indicates, for the first time, that PPARs are engaged in P4 and E2 production in porcine endometrium. It is possible that the diverse receptivity of endometrial tissue to the PPAR ligands can be associated with the reproductive status of gilts.
K. Chojnowska, J. Czerwinska, T. Kaminski, B. Kaminska, G. Panasiewicz, A. Kurzynska, and I. Bogacka
Springer Science and Business Media LLC
Iwona Bogacka, Aleksandra Kurzynska, Marek Bogacki, and Katarzyna Chojnowska
VM Media SP. zo.o VM Group SK
Peroxisome proliferator-activated receptors (PPARs) belong to a ligand-dependent nuclear receptor family. In the past decade, numerous studies have revealed the presence and significance of PPARs in the reproductive system. PPARs are expressed at different levels of hypothalamic-pituitary-gonadal (HPG) axis. They are also present in the uterus as well as in the placenta and embryonic tissues of different species. PPARs significance has been reported during the estrous/menstrual cycle and pregnancy with the gamma isoform studied most frequently. Several studies indicate that PPARs regulate proliferation of ovarian cells, tissue remodeling and steroidogenesis. In the endometrium, PPARs are engaged in the regulation of prostaglandins, steroids and cytokines synthesis. The role of PPARs in the trophoblast differentiation, maturation and invasion as well as in the embryo development has also been demonstrated. In this review, we summarize current findings concerning the role of PPARs in the regulation of reproductive functions at different levels of the HPG axis during various physiological statuses of females. In addition, the role of PPARs in the modulation of uterine functions as well as the placenta and embryo development has also been discussed.
I. Bogacka, M. Bogacki, A. Kurzyńska, and K. Chojnowska
Elsevier BV