Chu Hoang Mau
@tnue.edu.vn
Genetics&Biotechnology, Thai Nguyen University of Education, Vietnam
Thai Nguyen University of Education, Vietnam
RESEARCH, TEACHING, or OTHER INTERESTS
Biochemistry, Genetics and Molecular Biology, Biotechnology, Molecular Biology, Education
Scopus Publications
Scopus Publications
Yen Thi Hai Nguyen, Tan Quang Tu, Nhung Hong Nguyen, Doai Van Nguyen, Huyen Thi Tran, Phat Tien Do, Thu Thi Mai Lo, Nhan Thị Thanh Pham, Lan Thi Ngoc Nguyen, Thuy Thi Thu Vu,et al.
Springer Science and Business Media LLC
Thuy Thi Thu Vu, Lien Thi Kim Vu, Lam Tung Le, Thu Thi Mai Lo, and Mau Hoang Chu
MDPI AG
Ficus simplicissima Lour. is an Asian species of fig tree in the family Moraceae. The chloroplast (cp) genome of F. simplicissima m3 was sequenced using the Pacbio sequel platform. The F. simplicissima cpDNA has a size of 160,321 bp in length, of which GC content accounts for 36.13%. The cp genome of F. simplicissima consists of a single large copy (LSC) with a size of 91,346 bp, a single small copy (SSC) with a size of 20,131 bp, and a pair of inverted repeats with a size of 24,421 to 24,423 bp. The cp genome of F. simplicissima has 127 genes, including 85 protein-coding genes, eight rRNA genes, and 34 tRNA genes; 92 simple sequence repeats and 39 long repeats were detected in the cpDNA of F. simplicissim. A comparative cp genome analysis among six species in the Ficus genus indicated that the genome structure and gene content were highly conserved. The non-coding regions show more differentiation than the coding regions, and the LSC and SSC regions show more differences than the inverted repeat regions. Phylogenetic analysis supported that F. simplicissima m3 had a close relationship with F. hirta. The complete cp genome of F. simplicissima was proposed as a chloroplast DNA barcoding for genus-level in the Moraceae family and the psbA-trnH gene region for species-level identification.
Nga Thi Thu Nguyen, Hang Thi Thuy Pho, Quan Huu Nguyen, Nhung Thi Doan, Lan Thi Ngoc Nguyen, Huong Mai Pham, Lam Tung Le, Thuong Danh Sy, Ha Hoang Chu, Lien Thi Kim Vu,et al.
Springer Science and Business Media LLC
Tan Quang Tu, Phat Tien Do, Doai Van Nguyen, Nhan Thi Thanh Pham, Tam Thi Nguyen, and Mau Hoang Chu
Springer Science and Business Media LLC
Thi Ngoc Lan Nguyen, Thi Thu Hoan Hoang, Huu Quan Nguyen, Quang Tan Tu, Thi Hong Tran, Thi Mai Thu Lo, Thi Thu Thuy Vu, and Hoang Mau Chu
Springer Science and Business Media LLC
Tan Quang Tu, Phutthakone Vaciaxa, Thu Thi Mai Lo, Nhung Hong Nguyen, Nhan Thi Thanh Pham, Quan Huu Nguyen, Phat Tien Do, Lan Thi Ngoc Nguyen, Yen Thi Hai Nguyen, and Mau Hoang Chu
Elsevier BV
Huu Quan Nguyen, Thi Ngoc Lan Nguyen, Thi Nhung Doan, Thi Thu Nga Nguyen, Mai Huong Phạm, Tung Lam Le, Danh Thuong Sy, Hoang Ha Chu, and Hoang Mau Chu
Springer Science and Business Media LLC
AbstractAdrinandra megaphylla Hu is a medicinal plant belonging to the Adrinandra genus, which is well-known for its potential health benefits due to its bioactive compounds. This study aimed to assemble and annotate the chloroplast genome of A. megaphylla as well as compare it with previously published cp genomes within the Adrinandra genus. The chloroplast genome was reconstructed using de novo and reference-based assembly of paired-end reads generated by long-read sequencing of total genomic DNA. The size of the chloroplast genome was 156,298 bp, comprised a large single-copy (LSC) region of 85,688 bp, a small single-copy (SSC) region of 18,424 bp, and a pair of inverted repeats (IRa and IRb) of 26,093 bp each; and a total of 51 SSRs and 48 repeat structures were detected. The chloroplast genome includes a total of 131 functional genes, containing 86 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes. The A. megaphylla chloroplast genome indicated that gene content and structure are highly conserved. The phylogenetic reconstruction using complete cp sequences, matK and trnL genes from Pentaphylacaceae species exhibited a genetic relationship. Among them, matK sequence is a better candidate for phylogenetic resolution. This study is the first report for the chloroplast genome of the A. megaphylla.
Nhan Thi Thanh Pham, Dung Phuong Le, Khanh Thi Ngoc Pham, Xaykham Thipphavong, and Mau Hoang Chu
Open Science Publishers LLP
In Vietnam, some species in the genus Stephania are being overexploited and recorded in the Red Data Book of Vietnam. In this article, we present the identification results of Stephania spp. collected in Lào Cai province using morphological characteristics and the DNA barcode method to contribute to the conservation and exploitation of genetic resources and pharmaceuticals of this species in Vietnam. The analysis of the data shows that all morphology characteristics and comparative anatomy of the petioles, stems, and leaves are typical of Stephania brachyandra Diels species. The matK gene and ITS region isolated from Stephania_ Laocai have base lengths of 879 bp and 423 bp, respectively. The (Bbasic Llocal Aalignment Ssearch Ttool) analysis of the matK gene and ITS region obtained in this study has the highest similarity, 99.37% and 98.97%, respectively, to S. brachyandra species. The matK sequences were highly conserved and had low variable sites for 747 nucleotides (84.98%) and 132 nucleotides (15.29%), respectively, whereas the short ITS region was less conserved and had variable sites for 78 (18.44%) and 345 (81.56%), respectively. The results of the molecular phylogenetic analysis of the matK gene by the maximum likelihood method for the Stephania_Laocai sample showed that the matK sequence is suggested for better phylogenetic resolution than the ITS region and the combination of the matK gene and ITS region can be used to identify S. brachyandra species. Based on the combination of the characteristics of morphology and nucleotide sequences of the matK gene and ITS region, Stephania spp. collected in Lào Cai province of Vietnam were determined as S. brachyandra Diels.
Yen Thi Hai Nguyen, Hoan Thi Thu Hoang, Anh Thi Hoang Mai, Lan Thi Ngoc Nguyen, Quan Huu Nguyen, Nhan Thi Thanh Pham, Thuong Danh Sy, and Mau Hoang Chu
MDPI AG
Aconitum carmichaelii Debx. is a herbal species that contains many precious bioactive substances, which are alkaloids, flavonoids, steroids, and glycosides. Flavonoids, which are major secondary compounds, play an important role in maintaining redox balance in the cells of the plant body. Many flavonoids have antibacterial, antioxidant, and anticancer properties. However, studies have mainly focused on aconitine, which is a highly toxic group A poison belonging to the alkaloid group, but with little mention of flavonoids. The flavonoids in A. carmichaelii are a group of substances with high content, concentrated in leaves and flowers, including quercetin and kaempferol. F3′5′H (Flavonoid 3′5′-hydroxylase) has been identified as the key enzyme involved in the final steps of flavonoid biosynthesis in plants in general and in A. carmichaelii specifically. This study offers the first report, and demonstrates that the overexpression of the F3′5′H gene from a herbal plant, A. carmichaelii, increases flavonoid content in genetically modified tobacco plants. The A. carmichaelii gene was transformed into tobacco leaf tissue to create transgenic tobacco plants. The AcF3′5′H gene was incorporated into the tobacco genome and was expressed in four transgenic tobacco lines (T01, T03, T05, and T014). The F3′5′H content increased from 20.33% to 32.00% compared with that in non-transformed plants (P < 0.001). Therefore, the flavonoid content of four transgenic tobacco lines increased compared to the WT, from 69.23% to 122.23% (P < 0.001). The results of the successful expression of the AcF3′5′H gene in model tobacco plants are the basis for using the AcF3′5′H gene for improving flavonoid content in other medicinal plants. Thus, the AcF3′5′H gene considered in this work could be a candidate for gene technology to enhance flavonoid accumulation in plants.
Nga Thi Thu Nguyen, Lan Thi Ngoc Nguyen, Thuong Danh Sy, Quan Huu Nguyen, Trung Quang Tu, Khang Van Pham, Tan Quang Tu, and Mau Hoang Chu
Informa UK Limited
Abstract Capparis trinervia (family Capparaceae) is a common wild plant distributed in various localities in the mountainous region of Vietnam. While essential oils have been found in the leaves and stems of C. trinervia, there are no reports to date describing the chemical composition and biological activities to guide further exploration and application of the oils. This study determined the essential oil composition of C. trinervia; 8 and 23 distinct essential oil compounds were identified from C. trinervia leaves and stems, respectively. Stigmasterol (C29H48O) was identified as the primary compound of essential oil from leaves, accounting for 75.775% of the oils, while 3,7,11,15-tetramethyl-2-hexadecen-1-ol (C20H40O) was identified as the main essential oil component in C. trinervia stems, accounting for 18.337%. This study also explored the cytotoxic effects of essential oils extracted from C. trinervia against five cancer cells lines (A549, MCF7, HeLa, HepG2 and KB). A strong cytotoxic effect on all cancer cell lines was demonstrated by 100 µg mL−1 essential oils derived from C. trinervia leaves and stems, which suggests the species is a promising candidate for use in life and public healthcare.
Huu Quan Nguyen, Thi Hong Trang Le, Thi Ngoc Lan Nguyen, Thu Giang Nguyen, Danh Thuong Sy, Quang Tan Tu, Thi Thu Thuy Vu, Van Son Le, Hoang Mau Chu, and Thi Kim Lien Vu
Springer Science and Business Media LLC
Thi Hang Nguyen, Huu Quan Nguyen, and Hoang Mau Chu
Society for Research and Knowledge Management
In Vietnam, experiential activities and experience-based learning models were first introduced in 2018 into the general education curriculum and they will be applied when implementing this program. Therefore, the teacher training program at university of education should meet the requirements of general education reform. In particular, it is essential to guide pedagogical students to design and organise experiential learning activities in the school. This study describes the design, implementation, and evaluation of a course of guiding to design and organise experience-based learning in schools for Vietnamese pedagogical students who will plan to implement experience-based learning activities into their teaching. A course in a 60-hour instructional process (including 30 hours of self-studying) with 5 activities designed to be experiential, enables our students to simultaneously explore and practice experience-based learning activities. The results of designing and organising 55 experience-based learning activity plans were obtained from one hundred and nine participants of the Faculty of Biology, Thai Nguyen University of Education, Vietnam who assessed the instructional process beneficially. The research group also proposed five effective measures to organise experiential learning in high school. This tutorial process will continue to be improved and implemented in subsequent courses to contribute to the renovation of higher education and meet the general educational improvement in Vietnam.
Thi Thanh Nhan Pham, Huu Quan Nguyen, Thi Ngoc Lan Nguyen, Xuan Tan Dao, Danh Thuong Sy, Van Son Le, and Hoang Mau Chu
Southern Cross Publishing
The dehydration responsive element binding (DREB) is a plant protein subfamily expressed when soybean plants face abiotic stresses. These DREB proteins are also considered to activate the transcription of drought-resistant genes. In this study, we present the determined results of relationships between overexpression of Glycine max DREB2 (GmDREB2) with the transcription level of Glycine max pyrroline-5-carboxylate synthetase (GmP5CS) gene, proline accumulation and drought tolerant ability transgenic soybean plants as the basis for selection of transgenic lines with high drought tolerance. GmDREB2 was inserted into a plant transgenic vector and the 35S-GmDREB2-cmyc construct was transferred into the soybean plants by Agrobacterium-mediated transformation. Recombinant GmDREB2 protein with a molecular weight of approximately 20 kDa was expressed in four transgenic soybean lines in the T1 generation. The GmP5CS gene was shown to have significantly (P<0.05) increased expression in the T2 transgenic soybean lines and higher than compared to non-transgenic plants with considering both in well watered condition and stressed conditions were from 1.06 to 1.31-fold and 1.37 to 1.95-fold, respectively. The proline content of four transgenic soybean lines increased 155.81% to 187.42 % after five days in drought-stress conditions and increased from 180.52 % to 233.74 % after nine days under drought-stress conditions (P<0.05). Therefore, the overexpression of GmDREB2 resulted in increasing transcription level of P5CS gene, proline accumulation and drought-stress tolerance of the transgenic soybean plants. The GmDREB2 transformation into soybean plants was confirmed by the results of genetically modified lines in the T2 generation (T2-1, T2-6, T2-7, and T2-8) with higher drought tolerant ability than those of non-transgenic plants.
Danh Thuong Sy, Do Van Hai, Ritesh Kumar Choudhary, The Bach Tran, Hoang Mau Chu, Huu Quan Nguyen, Thi Thu Nga Nguyen, Gordon C. Tucker, and Joongku Lee
Pensoft Publishers
Capparis kbangensis Sy &amp; D.V. Hai, a new species from Kbang District, Gia Lai Province, Vietnam, is described and illustrated. The new species is morphologically similar to Capparis versicolor but differs by several characters such as emarginate leaf apex, hairy margin of sepals, smaller fruits, and fewer seeds per fruit. Its ecology and conservation status are provided along with a taxonomic key to the closely allied species.
Quan Huu Nguyen, Lien Thi Kim Vu, Lan Thi Ngoc Nguyen, Nhan Thi Thanh Pham, Yen Thi Hai Nguyen, Son Van Le, and Mau Hoang Chu
Springer Science and Business Media LLC
AbstractSoybean plants are sensitive to the effects of abiotic stress and belong to the group of crops that are less drought and salt tolerant. The identification of genes involved in mechanisms targeted to cope with water shortage is an essential and indispensable task for improving the drought and salt tolerance of soybean. One of the approaches for obtaining lines with increased tolerance is genetic modification. The dehydration-responsive element binding proteins (DREBs), belonging to the AP2 family, are trans-active transcription factors that bind to the cis-sequences of the promoter for activating the expression of the target genes that mediate drought and salt tolerant responses. In this study, the GmDREB6 transgene was introduced into DT84 cultivar soybean plants, using Agrobacterium-mediated transformation. The efficacy of GmDREB6 overexpression in enhancing the transcriptional level of GmP5CS and proline accumulation in genetically modified (GM) soybean plants was also assayed. The results demonstrated that ten GM soybean plants (T0 generation) were successfully generated from the transformed explants after selecting with kanamycin. Among these plantlets, the presence of the GmDREB6 transgene was confirmed in nine plants by Polymerase Chain Reaction (PCR), and eight plants showed positive results in Southern blot. In the T1 generation, four GM lines, labelled T1-2, T1-4, T1-7, and T1-10, expressed the recombinant GmDREB6 protein. In the T2 generation, the transcriptional levels of the GmP5CS gene were higher in the GM lines than in the non-transgenic plants, under normal conditions and also under conditions of salt stress and drought, ranging from 1.36 to 2.01 folds and 1.58 to 3.16 folds that of the non-transgenic plants, respectively. The proline content was higher in the four GM soybean lines, T2-2, T2-4, T2-7, and T2-10 than in the non-transgenic plants, ranging from 0.82 μmol/g to 4.03 μmol/g. The proline content was the highest in the GM T2-7 line (7.77 μmol/g). In GM soybean lines, T2-2, T2-4, T2-7, and T2-10 proline content increased after plants were subjected to salt stress for seven days, in comparison to that under normal conditions, and ranged from 247.83% to 300%, while that of the non-GM plants was 238.22%. These results suggested that GmDREB6 could act as a potential candidate for genetic engineering for improving tolerance to salt stresses.
Thi Thanh Nhan Pham, Thi Ngoc Lan Nguyen, Thi Ha Bui, Huu Quan Nguyen, Thi Tam Nguyen, Van Son Le, and Hoang Mau Chu
Informa UK Limited
ABSTRACT Catharanthus roseus contains vincristine and vinblastine, which are outstanding drugs for cancer. In the biosynthetic pathways of terpenoid indole alkaloids (TIAs) in C. roseus, deacetylvindoline 4-O-acetyltransferase (DAT) is a key enzyme that catalyses the last reaction of vindoline biosynthesis to form vinblastine and vincristine. In this study, the CrDAT transgene was transferred into the periwinkle by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The C. roseus DAT gene was introduced into C. roseus plants and it was confirmed that CrDAT was successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein. Four transgenic periwinkle lines in T1 generation, T1-1, T1-3, T1-6, and T1-7, expressed recombinant DAT protein with the total protein content in the range of 2.86 μg.mg−1 to 5.12 μg.mg−1. Moreover, the vincristine contents of four transgenic lines increased by 1.63−2.48-fold compared to non-transgenic plants, ranging from 6.91 µg.g−1 (fresh weight) to 10.53 µg.g−1 (fresh weight). The T1-1 line had the highest vincristine content. Hence, the overexpression of the recombinant DAT protein can improve the vincristine accumulation of transgenic C. roseus plants. Abbreviation: CrDAT - Catharanthus roseus Deacetylvindoline-4-O-Acetyl Transferase; D4H - Deacetoxyvindoline 4-hydroxylase; ELISA - Enzyme-Linked Immunosorbent Assay Monoterpene indole alkaloid; T0, T1 - Generations of transgenic plants; TIAs - Terpenoid indole alkaloids; WT- The wild-type tobacco plants (non transgenic plant); 35S - Cauliflower mosaic virus 35S promoter
Thi Xuan Thuy Vi, Thi Ngoc Lan Nguyen, Thi Thanh Nhan Pham, Huu Quan Nguyen, Thi Hai Yen Nguyen, Quang Tan Tu, Van Son Le, and Hoang Mau Chu
Springer Science and Business Media LLC
Thuy Thi Thu Vu, Lien Thi Kim Vu, Quan Huu Nguyen, Khang Van Pham, Dung Tien Nguyen, Lan Thi Ngoc Nguyen, and Mau Hoang Chu
Informa UK Limited
Abstract Extracts from the rhizomes of Paris species have been used in Asia to help treat inflammation, infection, analgesia, detoxification, dysentery, etc. Analysis of the chemical components from the rhizomes of Paris vietnamensis showed that they contained polyphenols, flavons, flavonols, flavonoids, tannins and steroidal and triterpenoid saponins. In this study, P. vietnamensis was identified from the sample collected from Sapa, Laocai province, Vietnam and three steroidal glycosides from rhizomes of this species were evaluated for anticancer and antimicrobial activity in vitro. The samples were identified as belonging to P. vietnamensis species through a combination of morphological characteristics and DNA barcodes based on the nucleotide sequence of the ITS and matK. All three steroidal glycosides exhibited strong cytotoxic activity against SK-LU-1, HeLa and MKN7 cell lines with IC50 values ranging from 1.07 to 4.37 µmol L−1, and exhibited high antimicrobial activity against strains of Serratia marcescens, Escherichia coli, Lactobacillus plantarum and Bacillus subtilis. This study provides information on the antibacterial and inhibitory activities of steroidal glycosides derived from the rhizomes of P. vietnamensis collected from Sapa, Laocai province, Vietnam on cancer cell lines. Thus, P. vietnamensis could be used as an alternative to other species of the Paris genus for anticancer and antimicrobial treatment.
Huu Quan Nguyen, Van Hanh Vu, Phuong Dung Le, and Hoang Mau Chu
Springer Science and Business Media LLC
Bui Thi Ha, , Nguyen Thi Ngoc Lan, Nguyen Thi Tam, Le Van Son, Chu Hoang Mau, , , , ,et al.
Southern Cross Publishing
'Catharanthus roseus' (L.) G. Don contains about 130 types of alkaloids, including vincristine and vinblastine, which are outstanding drugs for cancer. The 'C. roseus' deacetylvindoline-4-O-acetyl transferase (CrDAT) is a key enzyme which catalyzes the second to the final reactions in the vindoline way. The low content of indole alkaloid in 'C. roseus' plants and the high cost of indole alkaloid production have promted many research to improve indole alkaloid yield in this plant. The aim of this work was to express recombinant CrDAT in tobacco, a model plant, to create the basis for the overexpression of the gene encoding CrDAT ('GenBank' LN809930) in 'C. Roseus' plants. In this study, the 35S-DAT-cmyc structure was transferred to tobacco and the transgenic tobacco lines was generated. The T1 generation was then analyzed by Western blot method and ELISA analysis. Southern blot assays confirmed that the CrDAT gene was completely introduced into tobacco genome by Agrobacterium-mediated transformation. The recombinant CrDAT protein of 51.5 kDa in size was successfully expressed at the seven transgenic tobacco lines. The recombinant CrDAT protein content of transgenic tobacco lines were 2.75 - 5.35 (mu g. mg-1 of total protein) range and the recombinant CrDAT protein content of the T0-1 line was highest (5.35 mu g. mg-1 of total protein).
Sy Danh Thuong, Ritesh Kumar Choudhary, Gordon C. Tucker, Chu Hoang Mau, Thi Thu Nga Nguyen, Huu Quan Nguyen, and Joongku Lee
Finnish Zoological and Botanical Publishing Board
Capparis bachii Sy, R.K. Choudhary & Joongku Lee (Capparaceae) is described and illustrated based on a collection from Dong Nai Province, southern Vietnam. It is morphologically similar to C. diffusa but differs from it by having numerous flowers per umbel, numerous stamens per flower, larger gynophore, cordate leaf base, and presence of hairs on leaves.
Thi Nhu Trang VU, Thi Hong Trang LE, Phu Hiep HOANG, Danh Thuong SY, Thi Thu Thuy VU, and Hoang Mau CHU
The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS
* Correspondence: chuhoangmau@tnu.edu.vn
Sy Danh Thuong, Ritesh Kumar Choudhary, Tran The Bach, Do Van Hai, Bui Hong Quang, Gordon C. Tucker, Chu Hoang Mau, Joongku Lee, Changyoung Lee, and Sangmi Eum
Wiley
Capparis dongvanensis (Capparaceae) is described as a new species and illustrated from Ha Giang Province of Vietnam. It is morphologically similar to C. lanceolaris and C. fengii, but differs in the number of flowers on peduncles, length of spine and petiole, apex of the leaf, type of inflorescence, petal color, cylindrical ovary without any beak, fruit shape and number of seeds per fruit. Diagnostic morphological characters along with pollen and seed SEM images of C. dongvanensis and allied species are provided. Its ecology, habitat and conservation status are also described.
Thi Xuan Thuy VI, Hoang Duc LE, Vu Thanh Thanh NGUYEN, Van Son LE, and Hoang Mau CHU
The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS
Lo Thi Mai Thu, Vi Thi Xuan Thuy, Le Hoang Duc, Le Van Son, Chu Hoang Ha, and Chu Hoang Mau
FapUNIFESP (SciELO)
Soybean mosaic virus (SMV) and bean yellow mosaic virus (BYMV) are two typical types of viruses that cause mosaic in soybean plants. Multiple viral infections at the same site can lead to 66% to 80% yield reduction. We have aimed to improve SMV and BYMV resistance in Vietnamese soybeans using gene transfer techniques under the mechanism of RNAi. In this study, we present newly generated transgenic tobacco plants carrying RNAi [CPi (SMV-BYMV)] resistance to the two types of viruses; 73.08% of transgenic tobacco lines proved to be fully resistant to SMV and BYMV. In addition, the number of virus copies in transgenic tobacco plants was reduced on average by more than 51% compared to the control plants (wild type). This promising result shows the potential of transerring the CPi (SMV-BYMV) structure in soybean to increase resistance of soybean to SMV and BYMV and advance the aims of antiviral soybean breeding in Vietnam.