Dr. R.K. Garg

EDUCATION

My research interest focuses on molecular biology of the gene especially for animals intended for their
conservation purposes using certain molecular markers, Development of DNA barcodes database and
Development of molecular markers. Our team is also doing work on molecular based diseases diagnostics and
patterns identification for the populations of the Madhya Pradesh

RESEARCH, TEACHING, or OTHER INTERESTS

Animal Science and Zoology, Biotechnology, Molecular Biology, Ecology, Evolution, Behavior and Systematics

Scopus Publications

Molecular insights into the genetic and haplotype diversity among four populations of Catla catla from Madhya Pradesh revealed through mtDNA cyto b gene sequences
R.K. Garg, Vaishali Mishra
Journal of Genetic Engineering and Biotechnology, 2018
In the present investigation, the genetic structure of four populations of Catla catla, sequences of mitochondrial gene, cytochrome b (cyto b) from four populations were sequenced and analyzed. The sequences of mitochondrial regions revealed high haplotype diversity and low nucleotide diversity. The lowest 249 polymorphic sites and 0.00 parsimony informative sites were detected in populations of Fish Federation Pond (CCFFB) whereas highest 330 polymorphic sites and 56 parsimony informative sites were detected in populations of Narmada River (CCNRH) in the cyto b gene sequences in Catla catla populations. The twelve different haplotypes were detected among the four populations studied, lowest population specific haplotype as 2.00 was observed in Fish Federation Pond (CCFFB) and highest was in Population of Narmada River and Tighra reservoir. Sequencing of cyto b gene revealed 12 number of haplotypes (h) with haplotype (gene) diversity (Hd) 0.8736 and nucleotide diversity (π) 0.6474. These data clearly indicated that, feral/wild population showing highest values of polymorphisms, parsimony, haplotype diversity showing good, healthy habitat is lotic water (Narmada River) and lentic water body (Tighra reservoir). The results also concluded that the partial cyto b is polymorphic and can be a potential marker to determine ecological habitat based genetic differentiation among the populations.
Characterization of genetic variance within and among five populations of Sperata seenghala (Skyes, 1839) revealed by random amplified polymorphic DNA markers
R.K. Garg, P. Sairkar, S. Chouhan, N. Batav, N. Silawat, R. Sharma, R.K. Singh, N.N. Mehrotra
Journal of Genetic Engineering and Biotechnology, 2014
The genetic diversity among five populations (Bhadbada reservoir, Mohinisagar reservoir, Bansagar reservoir, Bargi reservoir and Gandhisagar reservoir) was revealed using random amplified polymorphic DNA markers. 10 random primers screened, 5 primers revealed various banding patterns and yielded 71 total loci as an average of which 39.60 (55.77%) were polymorphic between the population and 86.84% within the population of Sperata seenghala. Population wise the highest genetic polymorphism was obtained in Bhadbada reservoir as 67.61% whereas the lowest was in Gandhisagar reservoir as 49.30%. However, Analysis of Molecular Variance indicated low genetic diversity (Hpop = 0.0921 ± 0.1249; I = 0.1584 ± 0.1942) in Bansagar reservoir. Relative genetic differentiation (GST = 0.3993) and restricted gene flow (Nm = 0.7523) as an average indicated low gene diversity among the fish populations. The un-weighted pair group method with averages (UPGMA) dendrogram showed 05 major clusters, each cluster representing a population. Fish population of Mohinisagar reservoir showed high genetic distance (0.3981) with respective Bargi reservoir population and highest genetic identity (0.8846) reflected between Bansagar and Gandhisagar reservoir. Highest genetic distance between Mohinisagar and Bargi reservoir fish populations shows no significant correlation between genetic and geographical distance of the genotypes collected from different lentic and geographical isolated water bodies. This investigation indicated that lowest genetic diversity existed in different geographic populations of S. seenghala. All the five populations were found to be low in genetic variation, which is useful information for future conservation measures of S. seenghala confined in natural water bodies of Madhya Pradesh.
Fungal flora of vermicompost and organic manure: A case study of molecular diversity of mucor racemosus using RAPD analysis
Biosciences Biotechnology Research Asia, 2013
Assessment of genetic diversity of Clarias batrachus using RAPD markers in three water bodies of Bhopal
Journal of Environmental Biology, 2010
Genetic polymorphism of two populations of catfish Aorichthys seenghala (Sykes) using RAPD fingerprinting
International Journal of Integrative Biology, 2009
Genetic diversity between two populations of Heteropneustes fossilis (Bloch) using RAPD profile
R.K. Garg, P. Sairkar, N. Silawat, N. Vijay, N. Batav, N.N. Mehrotra
International Journal of Zoological Research, 2009
RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India
African Journal of Biotechnology, 2009
Genetic variability in Asparagus racemosus (Willd.) from Madhya Pradesh, India by random amplified polymorphic DNA
African Journal of Biotechnology, 2009
Water quality and conservation management of Ramsagar reservoir, Datia, Madhya Pradesh
Journal of Environmental Biology, 2009
Water quality and pollution status of Chambal river in National Chambal sanctuary, Madhya Pradesh
Journal of Environmental Biology, 2008
Checklist of fishes of Ramsagar reservoir, Datia district, Madhya Pradesh, India
R.K. Garg, R.J. Rao, D.N. Saksena
Zoos Print Journal, 2007
Assessment of physico-chemical water quality of Harsi reservoir, District Gwalior, Madhya Pradesh
Journal of Ecophysiology and Occupational Health, 2006