Evaluation of the Effectiveness of a Phytogenic Supplement (Alkaloids and Flavonoids) in the Control of Eimeria spp. in Experimentally Challenged Broiler Chickens Anne-Sophie Hascoët, Paulina Torres-Celpa, Roberto Riquelme-Neira, Héctor Hidalgo-Olate Animals, 2025 Avian coccidiosis remains a problematic challenge in poultry farms worldwide, with increasing concerns about resistance to conventional anticoccidial drugs, highlighting the need for alternative control strategies. A total of 256 male broilers (Ross 308), 1 day old, were randomly assigned to four experimental groups (eight replicates, each containing eight chickens) to evaluate the effectiveness of a phytogenic supplement against Eimeria spp. At 14 days, broilers were challenged via oral gavage with a mixture of six strains of live Eimeria spp. oocysts (4.0 × 104 Eimeria spp. oocysts per bird). The groups included a negative control (no anticoccidials or challenge), a positive control (coccidial challenge, no anticoccidials), a phytogenic-supplemented diet based on alkaloids and flavonoids, and a diet with anticoccidials (narasin and nicarbazin). Fecal samples were collected for oocyst counts from day 14 to 23, and at 23 days (9 days post-infection), intestinal lesions and mucosa measurements were evaluated. The oocyst counts in the phytogenic and anticoccidial groups were significantly lower than in the positive control (with a reduction of 61.3% and 71.6%, respectively, compared to the positive control) (p < 0.05), with no significant differences between the phytogenic supplement and anticoccidials. The phytogenic supplement provided intermediate protection based on histopathological scores with a significantly lower histopathology score than the positive control but significantly higher than the negative control (p < 0.05). No differences in body weight, feed intake, feed conversion ratio, or mortality were observed across groups during the trial, likely due to the moderate challenge applied. In conclusion, the phytogenic supplement demonstrated anticoccidial activity comparable to narasin and nicarbazin without compromising productivity, warranting further research into its mechanisms, resistance impact, and commercial application.
Effectiveness of enzymatic treatment for reducing dairy fouling at pilot-plant scale under real cleaning conditions Alfons Eduard Guerrero-Navarro, Abel Guillermo Ríos-Castillo, Carolina Ripolles-Avila, Anna Zamora, Anne-Sophie Hascoët, Xavier Felipe, Manuel Castillo, José Juan Rodríguez-Jerez Lwt, 2022 Conventional cleaning with chemical products uses a substantial amount of water and energy to clean the fouling related to dairy production, resulting in significant economic costs. We evaluated an enzymatic treatment based on the use of protease and amylase to clean dairy fouling generated in an indirect plate heat exchanger and the spray dryer equipment of a pilot plant, representing real cleaning conditions in the dairy industry. The efficacy of the enzymatic treatment in removing fouling at 50 °C was comparable to that of the clean-in-place method, with alkaline–acid cleaning performed at a maximum temperature of 80 °C. Microbiological analysis showed that the cleaning treatments guaranteed adequate hygienic conditions of the dairy products manufactured. Monitoring fluorescence markers, such as tryptophan, riboflavin, Maillard compounds, and dityrosine could help improve the effectiveness of both alkaline and enzymatic cleaning. The enzymatic treatment fulfills dairy industry objectives, saving water and energy during washing by reducing chemical product use. Considering that enzymatic cleaning is biodegradable after use and that its economic cost is competitive compared to chemical cleaning, it represents a viable alternative to the chemical cleaning of dairy fouling.
In vitro preformed biofilms of bacillus safensis inhibit the adhesion and subsequent development of listeria monocytogenes on stainless‐steel surfaces Anne-Sophie Hascoët, Carolina Ripolles-Avila, Brayan R. H. Cervantes-Huamán, José Juan Rodríguez-Jerez Biomolecules, 2021 Listeria monocytogenes continues to be one of the most important public health challenges for the meat sector. Many attempts have been made to establish the most efficient cleaning and disinfection protocols, but there is still the need for the sector to develop plans with different lines of action. In this regard, an interesting strategy could be based on the control of this type of foodborne pathogen through the resident microbiota naturally established on the surfaces. A potential inhibitor, Bacillus safensis, was found in a previous study that screened the interaction between the resident microbiota and L. monocytogenes in an Iberian pig processing plant. The aim of the present study was to evaluate the effect of preformed biofilms of Bacillus safensis on the adhesion and implantation of 22 strains of L. monocytogenes. Mature preformed B. safensis biofilms can inhibit adhesion and the biofilm formation of multiple L. monocytogenes strains, eliminating the pathogen by a currently unidentified mechanism. Due to the non-enterotoxigenic properties of B. safensis, its presence on certain meat industry surfaces should be favored and it could represent a new way to fight against the persistence of L. monocytogenes in accordance with other bacterial inhibitors and hygiene operations.
Microbial ecology evaluation of an iberian pig processing plant through implementing sch sensors and the influence of the resident microbiota on Listeria monocytogenes Anne-Sophie Hascoët, Carolina Ripolles-Avila, Alfons Eduard Guerrero-Navarro, José Juan Rodríguez-Jerez Applied Sciences Switzerland, 2019 There is a whole community of microorganisms capable of surviving the cleaning and disinfection processes in the food industry. These persistent microorganisms can enhance or inhibit biofilm formation and the proliferation of foodborne pathogens. Cleaning and disinfection protocols will never reduce the contamination load to 0; however, it is crucial to know which resident species are present and the risk they represent to pathogens, such as Listeria monocytogenes, as they can be further used as a complementary control strategy. The aim of this study was to evaluate the resident surface microbiota in an Iberian pig processing plant after carrying out the cleaning and disinfection processes. To do so, surface sensors were implemented, sampled, and evaluated by culture plate count. Further, isolated microorganisms were identified through biochemical tests. The results show that the surfaces are dominated by Bacillus spp., Pseudomonas spp., different enterobacteria, Mannheimia haemolytica, Rhizobium radiobacter, Staphylococcus spp., Aeromonas spp., lactic acid bacteria, and yeasts and molds. Moreover, their probable relationship with the presence of L. monocytogenes in three areas of the plant is also explained. Further studies of the resident microbiota and their interaction with pathogens such as L. monocytogenes are required. New control strategies that promote the most advantageous profile of microorganisms in the resident microbiota could be a possible alternative for pathogen control in the food industry. To this end, the understanding of the resident microbiota on the surfaces of the food industry and its relation with pathogen presence is crucial.
Evaluation of the Effectiveness of a Phytogenic Supplement (Alkaloids and Flavonoids) in the Control of Eimeria spp. in Experimentally Challenged Broiler Chickens AS Hascoët, P Torres-Celpa, R Riquelme-Neira, H Hidalgo-Olate Animals 15 (6), 847 , 2025 2025.0 Citations: 10
Evaluación del efecto anticoccidial de flavonoides cítricos en pollos de engorde AS Hascoët, P Torres, H Hidalgo Veterinaria 61 (1), 10 , 2025 2025.0
Effectiveness of enzymatic treatment for reducing dairy fouling at pilot-plant scale under real cleaning conditions AE Guerrero-Navarro, AG Ríos-Castillo, C Ripolles-Avila, A Zamora, ... Lwt 154, 112634 , 2022 2022.0 Citations: 27
In Vitro Preformed Biofilms of Bacillus safensis Inhibit the Adhesion and Subsequent Development of Listeria monocytogenes on Stainless-Steel Surfaces AS Hascoët, C Ripolles-Avila, BRH Cervantes-Huamán, ... Biomolecules 11 (3), 475 , 2021 2021.0 Citations: 15
Effect of an enzymatic treatment on the removal of mature Listeria monocytogenes biofilms: A quantitative and qualitative study T Mazaheri, C Ripolles-Avila, AS Hascoët, JJ Rodríguez-Jerez Food Control 114, 107266 , 2020 2020.0 Citations: 42
New approach for the removal of mature biofilms formed by wild strains of Listeria monocytogenes isolated from food contact surfaces in an Iberian pig processing plant C Ripolles-Avila, M Ramos-Rubio, AS Hascoët, M Castillo, ... International Journal of Food Microbiology 323, 108595 , 2020 2020.0 Citations: 35
Microbial ecology evaluation of an iberian pig processing plant through implementing SCH sensors and the influence of the resident microbiota on Listeria monocytogenes AS Hascoët, C Ripolles-Avila, AE Guerrero-Navarro, JJ Rodríguez-Jerez Applied Sciences 9 (21), 4611 , 2019 2019.0 Citations: 29
Hygienic properties exhibited by single-use wood and plastic packaging on the microbial stability for fish C Ripolles-Avila, AS Hascoët, AG Ríos-Castillo, JJ Rodríguez-Jerez LWT 113, 108309 , 2019 2019.0 Citations: 14
Development of a dairy fouling model to assess the efficacy of cleaning procedures using alkaline and enzymatic products AE Guerrero-Navarro, AG Ríos-Castillo, CR Avila, AS Hascoët, X Felipe, ... LWT 106, 44-49 , 2019 2019.0 Citations: 40
Evaluation of the microbiological contamination of food processing environments through implementing surface sensors in an iberian pork processing plant: An approach towards … C Ripolles-Avila, AS Hascoët, JV Martínez-Suárez, R Capita, ... Food Control 99, 40-47 , 2019 2019.0 Citations: 47
Quantification of mature Listeria monocytogenes biofilm cells formed by an in vitro model: A comparison of different methods C Ripolles-Avila, BH Cervantes-Huaman, AS Hascoët, J Yuste, ... International journal of food microbiology 289, 209-214 , 2019 2019.0 Citations: 45
Eficacia bactericida de nanopartículas de TiO2 activadas por luz UV contra bacterias Gram-positivas y Gram-negativas en suspensión CR Ávila, MM Garcia, AS Hascoët, JJR Jerez CyTA: Journal of food 17 (1), 408-418 , 2019 2019.0
Desarrollo de biofilms de listeria monocytogenes y microbiota acompañante en instalaciones cárnicas: cuantificación, acción sinérgica y antagonista AS Hascoët 2019.0 Citations: 3
Bactericidal efficacy of UV activated TiO 2 nanoparticles against Gram-positive and Gram-negative bacteria on suspension C Ripolles-Avila, M Martinez-Garcia, AS Hascoët, JJ Rodríguez-Jerez CyTA-Journal of Food 17 (1), 408-418 , 2019 2019.0 Citations: 61
Establishment of incubation conditions to optimize the in vitro formation of mature Listeria monocytogenes biofilms on food-contact surfaces C Ripolles-Avila, AS Hascoët, AE Guerrero-Navarro, JJ Rodríguez-Jerez Food Control 92, 240-248 , 2018 2018.0 Citations: 78
Growth inhibition of Listeria monocytogenes by a cell-free extract of cultured bacteria isolated from a meat processing plant AS Hascoët, C Ripolles, A Guerrero, M Martínez, JJ Rodríguez-Jerez Annals of Nutrition and Metabolism 73 (2), 79-79 , 2018 2018.0
Listeria monocytogenes and salmonella typhimurium biofilm detachment from stainless steel surfaces through enzymatic technology C Ripolles-Avila, AE Guerrero-Navarro, AS Hascoet, JJ Rodriguez-Jerez Annals of Nutrition and Metabolism 73 (2), 80-80 , 2018 2018.0 Citations: 1
DEMONSTRATION OF A NEW ENZYMATIC CLEANING PROCEDURE TO REMOVE MILK FOULING IN A DAIRY PILOT PLANT AE Guerrero-Navarro, AG Rios-Castillo, C Ripolles-Avila, AS Hascoët, ... Annals of Nutrition and Metabolism 73 (2), 84-84 , 2018 2018.0 Citations: 1
Reduction of animal excrement's potential as a polluant by using zeolite in feeding AS Hascoët 2016.0
Assessment of the anticoccidial effect of citrus flavonoids in broiler chickens AS Hascoët, P Torres-Celpa, H Hidalgo Sociedad de Medicina Veterinaria del Uruguay (SMVU) , 0
MOST CITED SCHOLAR PUBLICATIONS
Establishment of incubation conditions to optimize the in vitro formation of mature Listeria monocytogenes biofilms on food-contact surfaces C Ripolles-Avila, AS Hascoët, AE Guerrero-Navarro, JJ Rodríguez-Jerez Food Control 92, 240-248 , 2018 2018.0 Citations: 78
Bactericidal efficacy of UV activated TiO 2 nanoparticles against Gram-positive and Gram-negative bacteria on suspension C Ripolles-Avila, M Martinez-Garcia, AS Hascoët, JJ Rodríguez-Jerez CyTA-Journal of Food 17 (1), 408-418 , 2019 2019.0 Citations: 61
Evaluation of the microbiological contamination of food processing environments through implementing surface sensors in an iberian pork processing plant: An approach towards … C Ripolles-Avila, AS Hascoët, JV Martínez-Suárez, R Capita, ... Food Control 99, 40-47 , 2019 2019.0 Citations: 47
Quantification of mature Listeria monocytogenes biofilm cells formed by an in vitro model: A comparison of different methods C Ripolles-Avila, BH Cervantes-Huaman, AS Hascoët, J Yuste, ... International journal of food microbiology 289, 209-214 , 2019 2019.0 Citations: 45
Effect of an enzymatic treatment on the removal of mature Listeria monocytogenes biofilms: A quantitative and qualitative study T Mazaheri, C Ripolles-Avila, AS Hascoët, JJ Rodríguez-Jerez Food Control 114, 107266 , 2020 2020.0 Citations: 42
Development of a dairy fouling model to assess the efficacy of cleaning procedures using alkaline and enzymatic products AE Guerrero-Navarro, AG Ríos-Castillo, CR Avila, AS Hascoët, X Felipe, ... LWT 106, 44-49 , 2019 2019.0 Citations: 40
New approach for the removal of mature biofilms formed by wild strains of Listeria monocytogenes isolated from food contact surfaces in an Iberian pig processing plant C Ripolles-Avila, M Ramos-Rubio, AS Hascoët, M Castillo, ... International Journal of Food Microbiology 323, 108595 , 2020 2020.0 Citations: 35
Microbial ecology evaluation of an iberian pig processing plant through implementing SCH sensors and the influence of the resident microbiota on Listeria monocytogenes AS Hascoët, C Ripolles-Avila, AE Guerrero-Navarro, JJ Rodríguez-Jerez Applied Sciences 9 (21), 4611 , 2019 2019.0 Citations: 29
Effectiveness of enzymatic treatment for reducing dairy fouling at pilot-plant scale under real cleaning conditions AE Guerrero-Navarro, AG Ríos-Castillo, C Ripolles-Avila, A Zamora, ... Lwt 154, 112634 , 2022 2022.0 Citations: 27
In Vitro Preformed Biofilms of Bacillus safensis Inhibit the Adhesion and Subsequent Development of Listeria monocytogenes on Stainless-Steel Surfaces AS Hascoët, C Ripolles-Avila, BRH Cervantes-Huamán, ... Biomolecules 11 (3), 475 , 2021 2021.0 Citations: 15
Hygienic properties exhibited by single-use wood and plastic packaging on the microbial stability for fish C Ripolles-Avila, AS Hascoët, AG Ríos-Castillo, JJ Rodríguez-Jerez LWT 113, 108309 , 2019 2019.0 Citations: 14
Evaluation of the Effectiveness of a Phytogenic Supplement (Alkaloids and Flavonoids) in the Control of Eimeria spp. in Experimentally Challenged Broiler Chickens AS Hascoët, P Torres-Celpa, R Riquelme-Neira, H Hidalgo-Olate Animals 15 (6), 847 , 2025 2025.0 Citations: 10
Desarrollo de biofilms de listeria monocytogenes y microbiota acompañante en instalaciones cárnicas: cuantificación, acción sinérgica y antagonista AS Hascoët 2019.0 Citations: 3
Listeria monocytogenes and salmonella typhimurium biofilm detachment from stainless steel surfaces through enzymatic technology C Ripolles-Avila, AE Guerrero-Navarro, AS Hascoet, JJ Rodriguez-Jerez Annals of Nutrition and Metabolism 73 (2), 80-80 , 2018 2018.0 Citations: 1
DEMONSTRATION OF A NEW ENZYMATIC CLEANING PROCEDURE TO REMOVE MILK FOULING IN A DAIRY PILOT PLANT AE Guerrero-Navarro, AG Rios-Castillo, C Ripolles-Avila, AS Hascoët, ... Annals of Nutrition and Metabolism 73 (2), 84-84 , 2018 2018.0 Citations: 1
Evaluación del efecto anticoccidial de flavonoides cítricos en pollos de engorde AS Hascoët, P Torres, H Hidalgo Veterinaria 61 (1), 10 , 2025 2025.0
Eficacia bactericida de nanopartículas de TiO2 activadas por luz UV contra bacterias Gram-positivas y Gram-negativas en suspensión CR Ávila, MM Garcia, AS Hascoët, JJR Jerez CyTA: Journal of food 17 (1), 408-418 , 2019 2019.0
Growth inhibition of Listeria monocytogenes by a cell-free extract of cultured bacteria isolated from a meat processing plant AS Hascoët, C Ripolles, A Guerrero, M Martínez, JJ Rodríguez-Jerez Annals of Nutrition and Metabolism 73 (2), 79-79 , 2018 2018.0
Reduction of animal excrement's potential as a polluant by using zeolite in feeding AS Hascoët 2016.0
Assessment of the anticoccidial effect of citrus flavonoids in broiler chickens AS Hascoët, P Torres-Celpa, H Hidalgo Sociedad de Medicina Veterinaria del Uruguay (SMVU) , 0
