Ecology, Evolution, Behavior and Systematics, Insect Science, Biotechnology, Nature and Landscape Conservation
16
Scopus Publications
Scopus Publications
Synthesis and Characterization of Liposomes Derived from Oleaginous Yeast, Candida tropicalis Jordan Journal of Biological Sciences, 2025 Liposomes are nano or microvesicles used as carrier molecules for the transport of hydrophilic and hydrophobic substances.Oleaginous yeast is one of the potential source for the production of liposomes and hence an attempt has been made to produce liposomes from the lipids of oleaginous yeast, isolated from cultivation soil.The candidate species of oleaginous yeast producing elevated quantity of lipid (3.9U/ml in 96 hrs) was identified by 18s rRNA sequencing as Candida tropicalis.The lipids and phospholipids extracted were confirmed using FTIR spectroscopy and were used for the synthesis of liposomes by solvent injection method.The size of the liposome (78.7nm) was determined by AFM analysis.The HPLC analysis revealed the presence of phosphatidylcholine (0.44%) and ethanolamine (0.15%).The encapsulation efficiency was between 30 -50% as determined by UV-visible spectroscopy.Further research will throw light on the scope and applications of liposomes synthesized using Candida tropicalis in the biomedical and food industries.
WATER QUALITY ANALYSIS AND BIOMONITORING POTENTIAL OF AQUATIC INSECTS IN THE SELECTED STREAMS OF MUNNAR, KERALA Karuppaiya Sonaimuthu, S. Muralikrishnan, Marimuthu Muthukatturaja, Chellaiah Balasubramanian, Chellapandian Balachandran, et al. Indian Journal of Entomology, 2024 A total of 2,565 individuals of aquatic macroinvertebrates belonging to 27 genera, 18 families and seven orders were recorded from four streams of Munnar. Ephemeroptera was found to be the most dominant and diverse order. The Shannon and Simpson diversity indices were high in Suryanelli and low in Sivanmalai streams. The Evenness and Margalef indices were maximum in Periyavarai and Suryanelli streams and minimum in Sivanmalai stream. Water quality of the selected streams were analyzed with regard to the biotic indices. The functional feeding group (FFG) analysis revealed filter feeders as dominant group among FFGs in Munnar streams. The relationship between the water chemistry variables and aquatic macroinvertebrate community were discussed with canonical correspondence analysis (CCA).
Impact of rhizobacterium Bacillus sonorensis on propagation of Abelmoschus esculentus and its antimicrobial activity Balasundaram Harinathan, Subbiah Sankaralingam, Selvam Palpperumal, Chellapandian Balachandran, Abeer Hashem, Abdulaziz A. Alqarawi, Elsayed Fathi Abd_Allah, Selvaraj Arokiyaraj, Kathirvelu Baskar Journal of King Saud University Science, 2021 In order to evaluate the impact of Bacillus sonorensis on propagation of Abelmoschus esculentus and its antimicrobial activity was investigated. In the present study, A. esculentus was cultivated in B. sonorensis inoculated soil and also assessed the morphological as well as biochemical parameters of crops. The culture inoculum of B. sonorensis influenced growth and yield of treated plant. The root (39.5 cm), shoot length (20 cm), chlorophyll (1.06 mg), carotenoid (0.445 mg), protein and total soluble sugar content, NR & starch activity were higher in bioinoculated treated plant than control. The fresh biomass and dry weight were drastically increased in bioinoculated plants when compared to control. Gradual increase in composition of pivotal nutrients (N, P, K) and minor nutrients were observed in the bioinoculated plants. The culture filtrate possessed phytopathogenic activity against different phytopathogens. Among the three different phytopathogens, the maximum zone of inhibition (21 mm) was noticed in Aspergillus colletotrichum infected plants. The fermentation study was carried out in pilot scale fermentor and the synthesis of plant growth promoting substance was found to be 30.00 mg/l IAA.
Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes Chellapandian Balachandran, Alagumalai Vishali, Natarajan Arun Nagendran, Kathirvelu Baskar, Abeer Hashem, Elsayed Fathi Abd_Allah Saudi Journal of Biological Sciences, 2021 Marine microbes are potential source for novel metabolites. They are efficient in producing these metabolites utilizing agrowastes. Protease is one of the enzymes which find wide industrial applications. In the present study, protease producing bacteria was isolated from marine sediments and the organism was identified as Bacillus halodurans. The organism was subjected to protease production under solid state fermentation (SSF) using different agrowastes as substrates. Among the substrates used, wheat bran yielded maximum quantity of protease. The fermentation process was carried out under different cultural conditions to optimize the parameters influencing the enzyme production. The results of the stain removal studies by the enzyme revealed the increased efficiency of the microbial enzyme than the commercial detergent.
Biomedical and therapeutic potential of exopolysaccharides by Lactobacillus paracasei isolated from sauerkraut: Screening and characterization Thiruvengadam Shankar, Selvam Palpperumal, Durairaj Kathiresan, Subbiah Sankaralingam, Chellapandian Balachandran, Kathirvelu Baskar, Abeer Hashem, Abdulaziz A. Alqarawi, Elsayed Fathi Abd_Allah Saudi Journal of Biological Sciences, 2021 The intention of the study was evaluated for purification and characterization of exopolysaccharides from Lactobacillus paracasei; was isolated from homemade Sauerkraut sample collected from Sivakasi, Tamil Nadu, India, confirmed by biochemical and gene sequencing (16S rRNA). The purification and characterization of exopolysaccharides from candidate bacterium were studied on appearance, solubility of the EPS, carbohydrate estimation, emulsifying activity, sulphate, protein, uronic acid content, FTIR, HPLC and GC-MS analysis. The percentage of elemental carbon, (54.36%) hydrogen (21.74%), nitrogen (9.63%) and sulphur content (18.03%) were recorded in exopolysaccharides. The emulsification index (E24) of EPS was higher in toluene (79.20) and benzene (78.867) supplemented medium. FTIR spectrum of the candidate bacterial EPS confirmed presence of sulphate compounds, carboxyl group, and hydrogen bonded compounds etc. EPS exhibited 76.34% of Total Antioxidant Capacity (TAC), 71.15% of reducing power, 68.65% of Hydrogen Peroxide scavenging activity and also 60.31% DPPH radical scavenging activity. The potential antioxidant properties observed in exopolysaccharides from Lactobacillus paracasei is considered as valuable drugs.
Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae) Thiruvengadam Shankar, Subbiah Sankaralingam, Chellapandian Balachandran, Arunachalam Chinnathambi, Omaima Nasif, Sulaiman Ali Alharbi, Sungkwon Park, Kathirvelu Baskar Journal of King Saud University Science, 2021 The present work describes purification and characterization of an extracellular cellulase from a newly isolated cellulolytic bacterial strain Bacillus pumilus EWBCM1. The candidate strain was tested for its abilities to hydrolyze the structural polysaccharides through the depolymerising activities of carboxymethylcellulase. The purification of cellulase was carried out by ammonium sulphate precipitation, DEAE cellulose and sephadex G-100. Purified cellulase was found to be 47.56 fold along with 1.46 U/mg of protein and the molecular weight of cellulase was 47 kDa through SDS-PAGE electrophoresis. The optimum activity of cellulase was registered at pH 10 at 50 °C. In the present study, metal ions inferred the addition of 5 mM CaCl2 (128%) was recorded good activity than the other tested metal ions. Maximum activity of purified cellulase was recorded in 3% of NaCl and 5% of surfactants medium. The bioactive of purified cellulase was carried out with various commercial detergent and antimicrobial activity.
A new black fly species of Simulium (Nevermannia) (Simuliidae: Diptera) from the southern Eastern Ghats, India SANKARAPPAN ANBALAGAN, KALIMUTHU REKHA, SURULIYANDI VIJAYAN, CHELLAPANDIAN BALACHANDRAN, SUNDARAM DINAKARAN, MUTHUKALINGAN KRISHNAN Zootaxa, 2020 A new black fly species, Simulium (Nevermannia) karavalliense sp. nov. is described from Southern Eastern Ghats of South India. This new species is placed in the Simulium ruficorne species-group of the subgenus Nevermannia. S. (N.) karavalliense sp. nov. is characterized by female with a long sensory vesicle, male upper eye with 14 vertical columns and 16 horizontal rows of large facets, pupal gill with 8 filaments and larval hypostoma with 6 hypostomal bristles lying parallel to lateral margin. Taxonomic keys are provided to distinguish this new species from related species in India.
Two new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from South India SANKARAPPAN ANBALAGAN, SURULIYANDI VIJAYAN, CHELLAPANDIAN BALACHANDRAN, BERCHMANS THIYONILA, AATHMANATHAN SURYA Zootaxa, 2020 Two new black fly species, Simulium (Gomphostilbia) dinakarani sp. nov. and Simulium (Gomphostilbia) krishnani sp. nov. are described based on reared adult, pupal and larval specimens collected from the Western Ghats of India. These two new species are placed in the Simulium batoense species-group of the subgenus Gomphostilbia Enderlein. The morphological characters of two new species are compared with other related species of batoense species-group in India. The COI gene has successfully differentiated these two new species from its allies, S. (G.) panagudiense and S. (G.) takaokai of the batoense species group and the phylogenetic analysis by using COI gene sequences supporting its morphological classification.
