Yusuf Ahmed Yusuf Haggag
@tanta.edu.eg
Scopus Publications
- Correction: Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFβ/SMAD pathway (Scientific Reports, (2022), 12, 1, (6578), 10.1038/s41598-022-10494-4)
Mohamed A. Salem, Eman G. Manaa, Nada Osama, Nora M. Aborehab, Mai F. Ragab, et al.
Scientific Reports, 2026 - Design and preparation of novel domperidone loaded polymeric blend electrospun nanofibers for improved oral pharmacodynamic activity
Kamal Shatla, Eman Sweed, Suleiman Eltokhy, Adel Abdel-Rahman, Abdel Hamid Ismail, et al.
Drug Delivery and Translational Research, 2026
Domperidone (DOM) is a BCS class II drug which widely used as an oral medication for the control of nausea and vomiting. It showed a very low and variable bioavailability related to its very poor solubility and pre-systemic metabolism. Polymeric nanofibers (PNFs) exhibit unique properties that make them a favorable choice for drug delivery applications. The study aimed to develop an optimum fast-dissolving oral polymeric electrospun nanofiber of DOM using Eudragit L100/Polyvinyl alcohol (EL-100/PVA) polymer blend. The fabrication process was optimized through a set of formulation parameters (composition of the polymer blend, polymer blend concentration, and electrospinning voltage). The DOM-loaded EL-100/PVA NFs were characterized for surface shape, nanosize, % drug loading, DOM in vitro release, drug/polymer interaction, and in vivo prokinetic study. The optimization process showed defect-free DOM-loaded NFs with very low nano diameter, high DOM loading, and superior in vitro dissolution of DOM at intestinal pH (about 90% of the drug released within 5 min). The drug/polymer interaction study demonstrated the conversion of DOM into an amorphous form, which facilitated its dissolution. No physicochemical interaction between DOM and the polymer blend was observed. I n vivo prokinetic study signified the orally enhanced prokinetic activity of DOM-loaded NF relative to pure DOM and commercial DOM product. The DOM-loaded EL-100/PVA NFs exhibited a better effect on the gastrointestinal reactivity relative to free DOM and commercial DOM product. The superior in vitro dissolution and in vivo prokinetic activity proved the promising potential of polymeric NFs to improve the oral delivery of DOM. The optimized DOM-loaded EL-100/PVA NFs may allow for dose reduction and low cardiovascular risk compared to conventional DOM tablets. Graphical abstract - Facile Formulation of an Oral Nanovesicular Carrier Co-Encapsulating Simvastatin and Ezetimibe for Enhanced Lipid-Lowering Effect
Abdelrahman A. Elfarouny, Yusuf A. Haggag, Ebtessam A. Essa, Sanaa A. El-Gizawy
European Journal of Pharmaceutics and Biopharmaceutics, 2026 - Controlled delivery of STING agonist and manganese ion in injectable PLGA implants for enhanced cancer immunotherapy
Swetha Kodamasimham, Helen H. Ju, Xingwu Zhou, Yusuf Haggag, Clara Huang, et al.
Journal of Controlled Release, 2026 - Local controlled release of stabilized monoclonal antibodies
Rae Sung Chang, Jennifer Walker, Anzar A. Mujeeb, Padma Kadiyala, Karthik Pisupati, et al.
Journal of Controlled Release, 2025 - Formulation and characterization of novel oral pH-sensitive electrospun nanofibers for boosting dissolution and penetration of model class IV drug
Omar Y. Mady, Safaa Khaled, Asmaa A. Hedaya, Noorelhoda Abdine, Yusuf Haggag
Pharmaceutical Development and Technology, 2025
Sulpiride, (a model class IV drug) is a central dopamine antagonist, that is commonly used in the treatment of various psychiatric and gastrointestinal conditions. However, its poor aqueous solubility and low oral bioavailability (20-30%) limit its therapeutic efficacy. Electrospun nanofibers offer a promising method to enhance the oral absorption of poorly water-soluble drugs. This study, for the first time, aimed to investigate the feasibility of electrospun Eudragit S100-loaded sulpiride nanofibers as an enhanced oral delivery system, compared to drug-loaded solid dispersion. The physicochemical properties of the nanofibers were characterized. The drug's intestinal permeability was evaluated using an ex vivo non-everted sac technique. Sulpiride-loaded nanofibers exhibited uniform morphology with a very narrow nanosize range of (98.4-123.6 nm) and a high drug-loading efficiency of > 98%. In vitro, dissolution demonstrated a significant enhancement in the sulpiride's dissolution rates from the nanofiber formulations (> 94% of the drug released within 4 hours) compared to the solid dispersion formulation (˂77% of the drug released). The nanofiber formulations exhibited a 2-fold increase in the drug's intestinal permeability and a 4-fold increase in apparent permeability (Papp) compared to the free drug. The improved dissolution and intestinal permeability of sulpiride-loaded nanofibers suggest their potential application for enhancing the oral delivery and therapeutic efficacy of class IV drugs. - Enhanced intestinal permeation of novel sulpiride electrospun nanofibers: formulation, optimization, and ex vivo evaluation of drug absorption
Safaa Khaled, Omar Mady, Asmaa Hedaya, Noorelhoda Abdine, Yusuf Haggag
Drug Development and Industrial Pharmacy, 2025
SIGNIFICANCE Electrospinning presents a promising avenue for drug delivery applications by integrating traditional solid dispersion methods with nano-medicinal strategies. Electrospun nanofibers (NFs) can be tailored to control the composition, diameter, and orientation of the nanofibers based on the intended application. OBJECTIVES Herein, we aim to fabricate novel polymeric nanofibers loaded with sulpiride (SUL) utilizing Eudragit L100-55 (EL100-55) polymers to improve the dissolution and permeability of a model class IV drug. METHODS Various factors were assessed to optimize the electrospun nanofiber formulation, including polymer concentrations, flow rate, and drug amount. RESULTS The electrospinning process yielded defect-free sulpiride-loaded EL100-55 nanofibers. The physicochemical analysis demonstrated favorable attributes in all formulations, encompassing high drug loading, encapsulation efficiency, and rapid drug release. Nanofiber formulations exhibited superior dissolution due to their extensive surface area. Modified non-everted sac experiments revealed a twofold increase in sulpiride permeation through the intestinal membrane upon EL100-55 encapsulation, emphasizing its impact on tight junction modulation in both nanofiber and solid dispersion formulations. Enhanced drug permeation in the nanofiber formulation involved dual mechanisms: transcellular diffusion and widening of the paracellular pathway. In contrast, the solid dispersion formulation prepared via solvent evaporation predominantly widened the paracellular pathway. Visualization techniques illustrated the nanofibers' robust affinity for the transcellular pathway. CONCLUSIONS Sulpiride encapsulation into EL100-55-nanofiber is a promising solution for BCS class IV drugs facing solubility and permeability challenges. - Bioadhesive behaviors of HPMC E5: comparative analysis of various techniques, histological and human radiological evidence
Omar Y. Mady, Omar Dewedar, Noorelhoda Abdine, Hossam Zaytoon, Yusuf Haggag
Scientific Reports, 2024
Enhancing drug residence duration within the stomach offers distinct advantages for both localized and systemic effects. Numerous strategies have been proposed to extend drug residence time, with mucoadhesive polymers being a notable avenue. In this context, hydroxypropyl methylcellulose E5 has been employed as both a binding agent for granulating contrast metal powder and a mucoadhesive polymer, spanning various concentrations. The in vitro bioadhesion strength of the formulated tablets was gauged against the stomach lining of rabbits, for the quantification of bioadhesive forces. The temporal aspect of bioadhesion was evaluated through two approaches: one centered on gastric fluid dynamics and another proffered by the researchers, focusing on gastric wall kinetics. The results divulged a decline in bioadhesion force concomitant with high polymer concentrations. Histological examination of stained stomach sections revealed mucosal perturbations within the rabbit stomach. These disruptions exhibited an escalating trend in conjunction with elevated polymer concentrations, culminating in extensive disturbance at a 7.5% polymer concentration. The outcomes unveiled a direct relationship between polymer concentration increments and extended contact time. Subsequent radiological tracking of contrast metal behavior within a mature human stomach indicated a residence time of 6 h due to the entrapment of displaced components at disparate locations. - Co-delivery of novel valsartan and hydrochlorothiazide pH-responsive electrospun polymeric nanofibers for improved oral delivery
Yusuf A. Haggag, Mohamed Abdel-Wahab, Suleiman S. Eltokhy, Sally Elnawasany, Mokhtar M. Mabrouk, et al.
Journal of Drug Delivery Science and Technology, 2024 - Can the Adoption of Salvadora Persica among School-aged Learners Lead to Improved Oral Hygiene Practices?
International Journal of School Health, 2024 - In situ evaluation of the impact of metformin or verapamil coadministration with vildagliptin on its regional absorption from the rabbit’s intestine
Ahmed M. Elmeniar, Mohamed A. Osman, Sanaa A. El‐Gizawy, Dimple Modi, Nitin B. Charbe, et al.
Biopharmaceutics and Drug Disposition, 2024 - Anti-cancer effect of nano-encapsulated boswellic acids, curcumin and naringenin against HepG-2 cell line
Sally Elnawasany, Yusuf A. Haggag, Shahinaz M. Shalaby, Nema A. Soliman, Amira A. EL Saadany, et al.
BMC Complementary Medicine and Therapies, 2023 - Evaluation of the effect of guanabenz-loaded nanoparticles on chronic toxoplasmosis in mice
Wesam Moustafa Abdelhamid Elgendy, Yusuf A. Haggag, Kholoud A. El-Nouby, Samy I. El-Kowrany, Sirria M. El Marhoumy
Experimental Parasitology, 2023 - Fucoidan in Pharmaceutical Formulations: A Comprehensive Review for Smart Drug Delivery Systems
Yusuf Haggag, Abeer Abd Elrahman, Roland Ulber, Ahmed Zayed
Marine Drugs, 2023 - Bioavailability enhancement of acyclovir by honey: Analytical and histological evidence
Omar Y. Mady, Mohamed A. Osman, Naglaa I. Sarhan, Ali A. Shatla, Yusuf A. Haggag
Journal of Drug Delivery Science and Technology, 2023 - The adjunctive effect of locally delivered spirulina gel versus spirulina nanoparticles gel to nonsurgical treatment of stage II, grade B periodontitis: a randomized, controlled, clinical trial
Eman A. H. Altabrizy, Lobna A. A. ElGammal, Mohamed A. S. Attia, Yusuf A. Haggag, Ahmed M. M. Badr
Tanta Dental Journal, 2023 - Coriander (Coriandrum sativum L.) essential oil and oil-loaded nano-formulations as an anti-aging potentiality via TGFβ/SMAD pathway
Mohamed A. Salem, Eman G. Manaa, Nada Osama, Nora M. Aborehab, Mai F. Ragab, et al.
Scientific Reports, 2022 - Matrix metalloproteinase 2 is a target of the RAN-GTP pathway and mediates migration, invasion and metastasis in human breast cancer
Mohamed El-Tanani, Angela Platt-Higgins, Yin-Fai Lee, Arwa Omar Al Khatib, Yusuf Haggag, et al.
Life Sciences, 2022 - Advances in nanomaterial-based immunosensors for prostate cancer screening
Rabia Khan, Fareeha Arshad, Israr U. Hassan, Gowhar A. Naikoo, Mona Z. Pedram, et al.
Biomedicine and Pharmacotherapy, 2022 - PH-sensitive nanoparticles containing 5-fluorouracil and leucovorin as an improved anti-cancer option for colon cancer
Bakry Ibrahim, Omar Y Mady, Murtaza M Tambuwala, Yusuf A Haggag
Nanomedicine, 2022 - Fucoidans as nanoparticles: Pharmaceutical and biomedical applications
Ahmed Zayed, Yusuf Haggag, Shahira M. Ezzat, Mohamed A. Salem, Roland Ulber
Polysaccharide Nanoparticles Preparation and Biomedical Applications, 2022 - Overview of key molecular and pharmacological targets for diabetes and associated diseases
Seyed Hossein Shahcheraghi, Alaa A.A. Aljabali, Mazhar S. Al Zoubi, Vijay Mishra, Nitin B. Charbe, et al.
Life Sciences, 2021 - Repurposing of Guanabenz acetate by encapsulation into long-circulating nanopolymersomes for treatment of triple-negative breast cancer
Yusuf A. Haggag, Mohamed Yasser, Murtaza M. Tambuwala, Suleiman S. El Tokhy, Mohammad Isreb, et al.
International Journal of Pharmaceutics, 2021 - Preparation and In vitro characterization of a novel self-nano emulsifying drug delivery system for a fixed-dose combination of candesartan cilexetil and hydrochlorothiazide
Moataz B. Zewail, Sanaa A. El-Gizawy, Mohamed A. Osman, Yusuf A. Haggag
Journal of Drug Delivery Science and Technology, 2021 - Polymeric nanoencapsulation of zaleplon into PLGA nanoparticles for enhanced pharmacokinetics and pharmacological activity
Yusuf A. Haggag, Ahmed Kh. Abosalha, Murtaza M. Tambuwala, Enass Y. Osman, Sanaa A. El‐Gizawy, et al.
Biopharmaceutics and Drug Disposition, 2021 - The plausible mechanisms of tramadol for treatment of COVID-19
Nahla E. El-Ashmawy, Abdel-Halim A. Lashin, Kamal M. Okasha, Amal M. Abo Kamer, Tarek M. Mostafa, et al.
Medical Hypotheses, 2021 - Correction: Albumin nano-encapsulation of piceatannol enhances its anticancer potential in colon cancer via down regulation of nuclear p65 and hif-1ff (cancers 2020, 12, 113)
Alaa A. A. Aljabali, Hamid A. Bakshi, Faruck L. Hakkim, Yusuf A. Haggag, Khalid M. Al-Batanyeh, et al.
Cancers, 2020 - Is hesperidin essential for prophylaxis and treatment of COVID-19 Infection?
Yusuf A. Haggag, Nahla E. El-Ashmawy, Kamal M. Okasha
Medical Hypotheses, 2020 - Co-delivery of a RanGTP inhibitory peptide and doxorubicin using dual-loaded liposomal carriers to combat chemotherapeutic resistance in breast cancer cells
Yusuf Haggag, Bayan Abu Ras, Yahia El-Tanani, Murtaza M. Tambuwala, Paul McCarron, et al.
Expert Opinion on Drug Delivery, 2020 - Nanoencapsulation of sophorolipids in PEGylated poly(lactide-co-glycolide) as a novel approach to target colon carcinoma in the murine model
Yusuf Haggag, Mohamed Elshikh, Mohamed El-Tanani, Ibrahim M Bannat, Paul McCarron, et al.
Drug Delivery and Translational Research, 2020 - Design, formulation and in vivo evaluation of novel honokiol-loaded PEGylated PLGA nanocapsules for treatment of breast cancer
Yusuf A Haggag, Rowida R Ibrahim, Amin A Hafiz
International Journal of Nanomedicine, 2020 - Albumin nano-encapsulation of piceatannol enhances its anticancer potential in colon cancer via downregulation of nuclear p65 and HIF-1α
Alaa A. A. Aljabali, Hamid A. Bakshi, Faruck L. Hakkim, Yusuf A. Haggag, Khalid M. Al-Batanyeh, et al.
Cancers, 2020 - Novel ran-RCC1 inhibitory peptide-loaded nanoparticles have anti-cancer efficacy in vitro and in vivo
Yusuf Haggag, Kyle Matchett, Robert Falconer, Mohammad Isreb, Jason Jones, et al.
Cancers, 2019 - Polymeric nano-encapsulation of 5-fluorouracil enhances anti-cancer activity and ameliorates side effects in solid Ehrlich Carcinoma-bearing mice
Yusuf A. Haggag, Mohamed A. Osman, Sanaa A. El-Gizawy, Ahmed E. Goda, Maha M. Shamloula, et al.
Biomedicine and Pharmacotherapy, 2018 - Effect of poly(ethylene glycol) content and formulation parameters on particulate properties and intraperitoneal delivery of insulin from PLGA nanoparticles prepared using the double-emulsion evaporation procedure
Yusuf A. Haggag, Ahmed M. Faheem, Murtaza M. Tambuwala, Mohamed A. Osman, Sanaa A. El-Gizawy, et al.
Pharmaceutical Development and Technology, 2018 - Polymeric nano-encapsulation of curcumin enhances its anti-cancer activity in breast (Mda-mb231) and lung (a549) cancer cells through reduction in expression of hif-1αand nuclear p65 (rel a)
Mohammed N. Khan, Yusuf A. Haggag, Majella E. Lane, Paul A. McCarron, Murtaza M. Tambuwala
Current Drug Delivery, 2018 - Nano-encapsulation of a novel anti-Ran-GTPase peptide for blockade of regulator of chromosome condensation 1 (RCC1) function in MDA-MB-231 breast cancer cells
Yusuf A. Haggag, Kyle B. Matchett, El-Habib Dakir, Paul Buchanan, Mohammed A. Osman, et al.
International Journal of Pharmaceutics, 2017 - Preparation and in vivo evaluation of insulin-loaded biodegradable nanoparticles prepared from diblock copolymers of PLGA and PEG
Yusuf Haggag, Yasser Abdel-Wahab, Opeolu Ojo, Mohamed Osman, Sanaa El-Gizawy, et al.
International Journal of Pharmaceutics, 2016 - Sulindac solid dispersions: Development, characterization and in vivo evaluation of ulcerogenic activity in rats
Yusuf Haggag, Sanaa El-Gizawy, Esmat El-din, Nagla El-Shitany, Mohamed Osman
Journal of Applied Pharmaceutical Science, 2016 - Ran GTPase promotes cancer progression via Met receptor-mediated downstream signaling
Hiu-Fung Yuen, Ka-Kui Chan, Angela Platt-Higgins, El-Habib Dakir, Kyle B. Matchett, et al.
Oncotarget, 2016 - Evaluation of nano spray drying as a method for drying and formulation of therapeutic peptides and proteins
Yusuf A. Haggag, Ahmed M. Faheem
Frontiers in Pharmacology, 2015
