Ahmed Zayed
@webofscience.com
RESEARCH, TEACHING, or OTHER INTERESTS
Agricultural and Biological Sciences, Plant Science, Food Science
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Rehan M. El-Shabasy, Tarek F. Eissa, Yossef Emam, Ahmed Zayed, Nesrin Fayek, and Mohamed A. Farag
Springer Science and Business Media LLC
AbstractIncreasing attention has been given to mango (Mangifera indica) fruits owing to their characteristic taste, and rich nutritional value. Mango kernels are typically discarded as a major waste product in mango industry, though of potential economic value. The present study aims to outline the first comparison of different mango kernel cvs. originated from different localities alongside Egypt, e.g., Sharqia, Suez, Ismailia, and Giza. Gas chromatography–mass spectroscopy (GC–MS) post silylation analysis revealed that sugars were the major class being detected at 3.5–290.9 µg/mg, with some kernels originating from Sharqia province being the richest amongst other cvs. In consistency with sugar results, sugar alcohols predominated in Sharqia cvs. at 1.3–38.1 µg/mg represented by ribitol, iditol, pinitol, and myo-inositol. No major variation was observed in the fatty acids profile either based on cv. type or localities, with butyl caprylate as a major component in most cvs. identified for the first time in mango. Regarding phenolics, Sedeeq cv. represented the highest level at 18.3 µg/mg and showing distinct variation among cvs. posing phenolics as better classification markers than sugars. Multivariate data analyses (MVA) confirmed that the premium cvs “Aweis and Fons” were less enriched in sugars, i.e., fructose, talose, and glucose compared to the other cvs. Moreover, MVA of Zabdeya cv. collected from three localities revealed clear segregation to be chemically distinct. Sharqia originated mango kernels were rich in sugars (e.g., glucose and fructose), whilst sarcosine esters predominated in other origins.
Meryem El Jemli, Shahira M. Ezzat, Mourad Kharbach, Eman Sherien Mostafa, Rasha Ali Radwan, Yousra El Jemli, Otman El-Guourrami, Samir Ahid, Yahia Cherrah, Ahmed Zayed,et al.
Elsevier BV
Andrea Schmeckebier, Christian Ebel, Janik Haffelder, Christine Müller-Renno, Christiane Ziegler, Ahmed Zayed, Judith Stiefelmaier, and Roland Ulber
Springer Science and Business Media LLC
AbstractIn plant cell culture technology, plant cells grow in large agglomerates. For various investigations, however, single cells are required. One important parameter is the adhesion strength of single cells to surfaces or to each other. Hereby, e.g. the influence of the surface material and structure is of interest. To assess the adhesion forces of single cells to a surface, measurements with an atomic force microscope (AFM) can be applied. In the case of the plant cells Ocimum basilicum CMC, these cells grow as large cell agglomerates, so that a direct AFM single cell measurement is not possible. By developing a suitable cell separation method, it was possible to obtain vital single cells of the Ocimum basilicum CMC type and also to carry out adhesion measurements of these cells on a glass surface. No explicit methods for cell separation of plant cell cultures could be found in the literature. This method is therefore a new innovation that could also be used for other cell lines.
Javad Sharifi‐Rad, Maria Magdalena Quetglas‐Llabrés, Antoni Sureda, Lorena Mardones, Marcelo Villagran, Eda Sönmez Gürer, Jelena Živković, Shahira M. Ezzat, Ahmed Zayed, Safa Gümüşok,et al.
Wiley
AbstractMetabolic syndrome (MetS) is a common disorder involving a cluster of metabolic abnormalities, such as abdominal obesity, hypertension, dyslipidemia, insulin resistance, and atherogenic profile. MetS is characterized by an increase in oxidative stress and a chronic proinflammatory state, which are directly related to the development and progression of this pathology. It has been seen how a healthy lifestyle and good dietary practices are key to improving the different metabolic parameters and, therefore, play a fundamental role in reducing the risk of developing diabetes. The present review focuses on the research evidence related to the therapeutic properties of Lycium barbarum L. in MetS gathered in the last years. Several preclinical studies suggest that L. barbarum extracts are a good dietary supplement for the prevention of cardiovascular diseases in people with MetS. This compound has been used for years in traditional Chinese medicine for the treatment of atrophic gastritis, problems related to the lungs, kidneys, and liver, and as a supplement for eye health. In addition, different in vitro and in vivo studies have been carried out that support the properties attributed to metabolites derived from L. barbarum, such as polysaccharides that have been shown diverse biological activities. In conclusion, L. barbarum extracts have multiple benefits to increase general well‐being and immune function. However, there are a limited number of studies related to effect of L. barbarum in MetS, but they demonstrated effectiveness in the treatment of obesity, diabetes mellitus type 2, and prevention of diabetes mellitus type 2 complication.
Ahmed Zayed, Hang Thi Thuy Cao, Vo Thi Dieu Trang, and Roland Ulber
Springer Science and Business Media LLC
Ahmed Zayed, Dalal A. Al-Saedi, Emmanuel Ofosu Mensah, Osman Nabayire Kanwugu, Parise Adadi, and Roland Ulber
MDPI AG
Fucoidan is a class of multifunctional polysaccharides derived from marine organisms. Its unique and diversified physicochemical and chemical properties have qualified them for potential and promising pharmacological uses in human diseases, including inflammation, tumors, immunity disorders, kidney diseases, and diabetes. Physicochemical and chemical properties are the main contributors to these bioactivities. The previous literature has attributed such activities to its ability to target key enzymes and receptors involved in potential disease pathways, either directly or indirectly, where the anionic sulfate ester groups are mainly involved in these interactions. These findings also confirm the advantageous pharmacological uses of sulfated versus non-sulfated polysaccharides. The current review shall highlight the molecular targets of fucoidans, especially enzymes, and the subsequent responses via either the upregulation or downregulation of mediators’ expression in various tissue abnormalities. In addition, in silico studies will be applied to support the previous findings and show the significant contributors. The current review may help in understanding the molecular mechanisms of fucoidan. Also, the findings of this review may be utilized in the design of specific oligomers inspired by fucoidan with the purpose of treating life-threatening human diseases effectively.
Maha M. Salama, Nora M. Aborehab, Nihal M. El Mahdy, Ahmed Zayed, and Shahira M. Ezzat
Springer Science and Business Media LLC
AbstractLeukemia is a group of malignant disorders which affect the blood and blood-forming tissues in the bone marrow, lymphatic system, and spleen. Many types of leukemia exist; thus, their diagnosis and treatment are somewhat complicated. The use of conventional strategies for treatment such as chemotherapy and radiotherapy may develop many side effects and toxicity. Hence, modern research is concerned with the development of specific nano-formulations for targeted delivery of anti-leukemic drugs avoiding toxic effects on normal cells. Nanostructures can be applied not only in treatment but also in diagnosis. In this article, types of leukemia, its causes, diagnosis as well as conventional treatment of leukemia shall be reviewed. Then, the use of nanoparticles in diagnosis of leukemia and synthesis of nanocarriers for efficient delivery of anti-leukemia drugs being investigated in in vivo and clinical studies. Therefore, it may contribute to the discovery of novel and emerging nanoparticles for targeted treatment of leukemia with less side effects and toxicities.
Islam M. Kamal, Ahmed Zayed, Tarek F. Eissa, and Mohamed A. Farag
Springer Science and Business Media LLC
AbstractGuava tree (Psidium guajava L., Myrtaceae) is an economic grown worldwide, particularly in tropical and subtropical regions. Guavas encompass numerous cultivars (cvs.) that were discriminated in previous studies based on leaf morphological features and profile of volatile organic compounds (VOCs). Nevertheless, fruit VOCs have also shown outstanding potential for discrimination of other plant taxa, which has not been utilized in guava. Hence, the current study investigates the various guava cvs. harvested from India and Egypt. A total of 5 samples were analyzed by solid phase microextraction coupled to gas chromatography/mass spectrometry. Results led to the detection of 42 VOCs belonging to aldehydes, alcohols, esters, ketones, aliphatic and aromatic hydrocarbons, in addition to monoterpene and sesquiterpene hydrocarbons. Butylated hydroxytoluene and β-caryophyllene were predominant reaching 77% and 41% in Egyptian and Indian guava, respectively. The impact of probiotic fermentation, i.e., Lactobacillus acidophilus and L. plantarum on aroma profile was not significantly different (p > 0.05). Multivariate data analyses were further applied for samples classification and markers determination, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). PCA score plot showed clear segregation of Egyptian from Indian specimens, whereas OPLS-DA revealed that β-caryophyllene was associated with white fruit versus 3-butenyl isothiocyanate and muurolol in red fruit type in the case of Indian guava. The richness of Egyptian guava in butylated hydroxytoluene in addition to the presence of vitamin C may potentiate its antioxidant activity, to be followed in subsequent studies regarding its health effects.
Nourhan Hisham Shady, Ahmed Zayed, Rania Alaaeldin, Mohamed Hisham, Mohamed Gawesh, Randa Mohammed, Mahmoud A. Elrehany, and Usama Ramadan Abdelmohsen
Elsevier BV
Ahmed Serag, Ahmed Zayed, Ahmed Mediani, and Mohamed A. Farag
Springer Science and Business Media LLC
AbstractTongkat ali commonly known as Malaysian Ginseng (Eurycomalongifolia) is a herbal root worldwide available in nutraceuticals, either as a crude powder or capsules blended with other herbal products. Herein, a multiplexed metabolomics approach based on nuclear magnetic resonance (NMR) and solid-phase microextraction combined with gas chromatography–mass spectrometry (SPME–GC–MS) was applied for authentic tongkat ali extract vs some commercial products quality control analysis. NMR metabolite fingerprinting identified 15 major metabolites mostly ascribed to sugars, organic and fatty acids in addition to quassinoids and cinnamates. Following that, multivariate analysis as the non-supervised principal component analysis (PCA) and supervised orthogonal partial least squares-discriminant analysis (OPLS-DA) were applied revealing that differences were related to fatty acids and 13,21-dihydroeurycomanone being more enriched in authentic root. SPME–GC–MS aroma profiling led to the identification of 59 volatiles belonging mainly to alcohols, aldehydes/furans and sesquiterpene hydrocarbons. Results revealed that aroma of commercial products showed relatively different profiles being rich in vanillin, maltol, and methyl octanoate. Whereas E-cinnamaldehyde, endo-borneol, terpinen-4-ol, and benzaldehyde were more associated to the authentic product. The present study shed the light for the potential of metabolomics in authentication and standardization of tongkat ali and identification of its true flavor composition.
Abdel-Rahim S. Ibrahim, Mai K. Mansour, Mohammed M.A. Ahmed, Roland Ulber, and Ahmed Zayed
Elsevier BV
Aya A. Abdella, Roland Ulber, and Ahmed Zayed
Elsevier BV
Ahmed Zayed, Hang Thi Thuy Cao, Vo Thi Dieu Trang, and Roland Ulber
Springer Science and Business Media LLC
Ahmed Zayed, Mohamed A. Farag, Alexander Mehring, Mohamed A. Salem, Rana M. Ibrahim, Saleh Alseekh, Alisdair R. Fernie, and Roland Ulber
Elsevier BV
Mohamed A. Farag, Ali Reda, Mohamed Nabil, Diaaeldin M. Elimam, and Ahmed Zayed
Royal Society of Chemistry (RSC)
Evening primrose oil has attracted considerable interest worldwide because of its bioactive constituents and numerous functions in foods and health.
Ahmed Zayed, Mohamed A. Salem, Walaa A. Negm, and Shahira M. Ezzat
Springer Science and Business Media LLC
Hend Mostafa Selim, Walaa A. Negm, Mohammed F. Hawwal, Ismail A. Hussein, Engy Elekhnawy, Roland Ulber, and Ahmed Zayed
Elsevier BV
Mohamed A. Salem, Hend E. Abo Mansour, Esraa M. Mosalam, Riham A. El-Shiekh, Shahira M. Ezzat, and Ahmed Zayed
Springer Science and Business Media LLC
Walaa A. Negm, Shahira M. Ezzat, and Ahmed Zayed
Royal Society of Chemistry (RSC)
Vector-borne diseases (VBDs) are a worldwide critical concern accounting for 17% of the estimated global burden of all infectious diseases in 2020.
Yusuf Haggag, Abeer Abd Elrahman, Roland Ulber, and Ahmed Zayed
MDPI AG
Fucoidan is a heterogeneous group of polysaccharides isolated from marine organisms, including brown algae and marine invertebrates. The physicochemical characteristics and potential bioactivities of fucoidan have attracted substantial interest in pharmaceutical industries in the past few decades. These polysaccharides are characterized by possessing sulfate ester groups that impart negatively charged surfaces, low/high molecular weight, and water solubility. In addition, various promising bioactivities have been reported, such as antitumor, immunomodulatory, and antiviral effects. Hence, the formulation of fucoidan has been investigated in the past few years in diverse pharmaceutical dosage forms to be able to reach their site of action effectively. Moreover, they can act as carriers for various drugs in value-added drug delivery systems. The current work highlights the attractive biopharmaceutical properties of fucoidan being formulated in oral, inhalable, topical, injectable, and other advanced formulations treating life-quality-affecting diseases. Therefore, the present work points out the current status of fucoidan pharmaceutical formulations for future research transferring their application from in vitro and in vivo studies to clinical application and market availability.
Aya Allam, Noha Shafik, Ahmed Zayed, Ibrahim Khalifa, Ibrahim A. Bakry, and Mohamed A. Farag
PeerJ
Hazard Analysis Critical Control Point (HACCP) is a risk management protocol developed to ensure food safety through a precautionary approach that is believed to offer assurances in producing safe food for customers. Yogurt is made in a number of phases, commencing with the collection of raw milk and ending with consumer consumption. While this is happening, major economic and health issues might arise from exposing the manufacturing line to biological, chemical, and/or physical contaminations. As a result, the decision tree approach was used to determine the CCPs during the production of yogurt. Additionally, biological dangers are incorporated as a by-product of the system’s implementation performance. In particular, the plain set and nut puree-honey-fortified stirred yogurt manufacturing techniques are highlighted for the first time in this study. The potential manufacturing risks are described for the first time, together with information on how HACCP plans may guard against major risks that could result in the production of yogurt that is not in compliance with established standards.
Mohamed A. Farag, Asmaa F. Aboul Naser, Ahmed Zayed, and Mohamed G. Sharaf El-Dine
MDPI AG
Interest in the consumption of seed sprouts is gradually increasing as functional foods in the modern Western diet owing to their several nutritional and health benefits. The present study aims to investigate four major legume sprouts derived from faba bean (Vicia faba L.), lentil (Lens esculenta L.), chickpea (Cicer arietinum L.), and fenugreek (Trigonella foenum-greacum L.) for their antidiabetic activity and mitigation of associated complications, i.e., oxidative stress, liver dysfunction, and lipid metabolism, compared with glibenclamide. Biochemical results presented herein further showed that the four sprouts exhibited significant hypoglycemic effects (p < 0.05), with improvement in decreasing of blood glucose levels at different degrees and with faba bean sprout most active at 348% improvement, compared to 364.3% for glibenclamide. Further biochemometric analysis based on a comparison between targeted versus untargeted partial least square (PLS) and regression analyses revealed that faba bean sprouts’ richness in flavonoids was a determinant key factor for such efficacy. In addition, correlation with previously investigated NMR fingerprinting aided in pinpointing other active agents, such as betaine and L-DOPA. Furthermore, the effect on serum liver enzymes, including alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase; oxidative stress markers; and lipid profiles showed significant improvement, especially in the case of faba bean sprout. The study revealed the potential health benefits of legume sprouts in the treatment of diabetes and its associated complications, as well as the potential role of biochemometrics in active agents’ identification in such a complex matrix to be considered for other functional foods investigation.
Ahmed Zayed, Doris Finkelmeier, Thomas Hahn, Lisa Rebers, Anusriha Shanmugam, Anke Burger-Kentischer, and Roland Ulber
MDPI AG
Microwave-assisted extraction (MAE) is recognized as a green method for extraction of natural products. The current research aimed to explore the MAE for fucoidans extraction from different brown seaweeds, including Fucus vesiculosus, F. spiralis, and Laminaria saccharina. Following several solvent-extraction pre-treatment steps and MAE optimization, the algal biomasses were extracted in a ratio of 1:25 in 0.1 M HCl containing 2 M CaCl2 for 1.0 min. The results showed that L. saccharina’s extract was different from the others, regarding the highest sugar content reached 0.47 mg glucose equivalent/mg extract being confirmed by monosaccharide composition analysis and the lowest fucoidan content and sulfation degree at 0.09 mg/mg extract and 0.13, respectively. Moreover, these findings were confirmed by tentative structural elucidation based on Fourier-transform infrared spectrometry which also showed a different spectrum. However, the MAE enhanced melanoidins formation in products, which was confirmed by the intense band at 1420 cm−1. Interestingly, the results of monomeric composition showed that fucoidan extract by MAE from F. vesiculosus belonged to sulfated galactofucans which are known for their potential bioactivities. Furthermore, the cytotoxic activity of the four fucoidans in concentrations ranging from 4.9 µg/mL to 2500 µg/mL was investigated and correlated with the chemical characterization showing that F. vesiculosus_MAE fucoidan was the most potent and safest. The current research revealed the chemical heterogeneity of fucoidans regarding taxonomical class and used greener extraction method of fucoidans toward the achievement of the UN sustainability goals.
Alyaa Hatem Ibrahim, Eman Zekry Attia, Heba A. Hofny, Faisal Alsenani, Ahmed Zayed, Mostafa E. Rateb, Usama Ramadan Abdelmohsen, Samar Yehia Desoukey, Mostafa Ahmed Fouad, and Mohamed Salah Kamel
Informa UK Limited
Abstract This work was performed to dig into the phytochemical composition and bioactivities of Nocardiopsis sp. UR67 associated with the marine sponge Callyspongia sp. It was fermented in suspension and immobilised in calcium alginate bead cultures. The ethyl acetate extracts, afforded from the broth in each case named EG-49 and J-48g, respectively, revealed 16 chemical principles mostly belonging to polyketides, macrolides, and peptides. EG-49 and J-48g displayed anti-Candida albicans activity with IC50 values of 8.1 and 8.3 µg/mL, and a substantial cytotoxic effect against lung adenocarcinoma H1650 at IC50 12.6 and 13.7 µg/mL, respectively. However, only EG-49 exhibited a noteworthy anti-trypanosomal activity at 7.5 µg/mL. Molecular docking of the characterised compounds against Trypanosoma brucei trypanothione reductase demonstrated the highest binding models of griseochelin-methyl ester (9) and filipin-II (11), which drew considerable significance of the metabolites derived from Nocardiopsis sp. UR67 developing potential T. brucei trypanothione reductase inhibitors.
Mohammed M. A. Ahmed, Ehab A. Ragab, Ahmed Zayed, Elsayed M. El-Ghaly, Said K. Ismail, Shabana I. Khan, Zulfiqar Ali, Amar G. Chittiboyina, and Ikhlas A. Khan
Informa UK Limited
Abstract Soft corals distributed across the Red Sea coasts are a rich source of diverse and bioactive natural products. Chemical probing of the Red Sea soft coral Litophyton arboreum led to isolation and structural characterization of an undescribed sesquiterpenoid, litoarbolide A (1), along with 14 previously reported metabolites (2-15). The chemical structures of the isolates were assigned based on NMR as well as high resolution electrospray ionization mass spectrometry (HR-ESI-MS) data. Litoarbolide A is supposed to be the biosynthetic precursor to other sesquiterpenoids, which formed via further post-translational modifications. Furthermore, these metabolites were evaluated for anti-malarial activity, where only the acyclic sesquiterpenoid of a sec-germacrane nucleus (7) showed an activity against chloroquine-sensitive (D6) and chloroquine-resistant (W2) strains of Plasmodium falciparum with IC50 at 3.7 and 2.2 mg/mL, respectively. Moreover, the isolated metabolites were all non-toxic to the Vero cell line. These findings support the consideration of L. arboreum in further natural anti-malarial studies. Graphical Abstract