Direct Impact of Pomegranate Juice and Peel Extracts on Prostate Cancer Progression - Inhibition of Proliferation, Migration, and Colony Formation in Prostate Cancer Cell Lines and Tingwei Li International Journal of Drug Delivery Technology, 2026 Prostate cancer, which affects millions of men worldwide, is a prevalent form of cancer. The risk factors associated with it include age, genetics, and diet. Despite advances in cancer research, the quest for effective treatments and preventive measures against this disease remains an ongoing challenge. Pomegranate, a fruit known for its antioxidant-rich juice and bioactive peel extracts, has been identified as a prospective therapeutic intervention for a range of health ailments, including cancer. The current study set out to look at the direct effects of the juice of pomegranates and peel extract on the advancement of prostate cancer. We evaluated their capacity to impede prostate cancer cell lines' colony formation, motility, and proliferation as cancer cells. To achieve this, the MTT test was used to measure the drop in the survival of cells that followed a PP extract injection. Prostate cancer cells' ability to form colonies and their ability to be inhibited from migrating were also evaluated using the clonogenic assay and wound healing experiment conducted with the help of pomegranate extracts. The results revealed promising inhibitory effects of pomegranate extracts on these key cancer hallmarks, suggesting their potential as complementary therapeutic agents for prostate cancer.
Role of nanosensors for sustainable agricultural practices Murugan Karuvelan, Soumya Lakshman, Usharani Boopathy, Ramachandran Chelliah, Deog Hwan Oh Agricultural Crop Improvement Plant and Soil Relationships, 2025 The agricultural sector serves as the primary foundation upon which the worldwide human population relies to fulfil their basic energy requirements. The current agricultural landscape is faced with significant challenges, mostly driven by the escalating global population and diminishing crop productivity. These challenges are primarily attributed to a multitude of biotic and abiotic stresses, which have emerged as key concerns in the field of agriculture. The process of urbanisation and modernisation in the agricultural sector has resulted in significant acceleration in the production of diverse raw materials. Nevertheless, the widespread use of chemical fertilisers and pesticides has been found to have detrimental consequences for both human health and the biodiversity of plant and animal species. The existing traditional procedures are inadequate for addressing the entirety of these difficulties. Therefore, it is imperative to explore alternate ways that are more efficient in response to immediate requirements. Within this particular context, the utilisation of nanotechnology (NT) has the potential to serve as an intelligent and efficient measure in the pursuit of sustainable agriculture. In recent decades, there has been significant growth in the field of NT. This advancement has led to the creation of efficient and cost-effective nanosensors (NSs) that can rapidly detect various contaminants present in natural agroecosystems. Importantly, these NSs have the added benefit of being safe for human health. The coalescence of NT and biotechnology has resulted in the creation of advanced NSs capable of detecting and diagnosing soil shortages and environmental conditions. This chapter centres on the examination of current applications, potential future prospects, and associated obstacles to integrating NT into agricultural practices.
Antidiabetic Potential of Emodin in Streptozotocin-Induced Type-2 Diabetic Rats Journal of Neonatal Surgery, 2025
Mukia maderaspatana (L.) M. Roem. phytoconstituents: Unveiling their anticancer potentials against hepatocellular carcinoma cells Sither Vasantha Kumar, Chandrasekar Shobana, Durairaj Rohini, Jegadeesan Manjunathan, Ramasamy Ramasubburayan, Natesan Thirumalaivasan, Santhiyagu Prakash, Boopathy Usharani South African Journal of Botany, 2024 Globally, hepatocellular carcinoma poses significant health challenges due to its higher incidence and mortality rates. Further adverse effects of synthetic drugs prompted research into herbal plants as safer, more effective and alternative treatments. Mukia maderaspatana (L.) M. Roem. is a traditional medicinal renowned for its therapeutic properties. This study explores the anticancer property of hydroalcoholic leaf extract of M. maderaspatana (HLEMM) against liver carcinoma (HepG2) cells and further unveils the active phytoconstituents. Purification and characterization of HLEMM through spectroscopic analysis (HPLC, UV-Vis, MS, FT-IR, NMR) evidenced 3,3′,4′,5,5′,7–Hexahydroxyflavone (Myricetin) as the active phytoconstituent. Cytotoxicity of HLEMM on HepG2 cells showed a significantly dose-dependent reduction in cell viability with an IC 50 of 128.6 ± 0.4 μg/ml. Cell cycle analysis revealed that HLEMM effectively inhibited HepG2 cells at the G2/M phase. Results of the Annexin-V/PI staining study inferred HLEMM-induced apoptosis in the early and late phases of HepG2 cells. Flow cytometry results confirmed the up-regulation of caspase-3 and caspase-9 along with the simultaneous down regulation of Bcl-2, substantiating the activation of apoptosis, a controlled process of programmed cell death due to cellular stress or damage. Depolarization of the mitochondrial membrane in HepG2 cells was confirmed by a decrease in JC-1 fluorescence, as observed under a confocal fluorescent microscope. This tends to indicate the alteration in membrane potential and interference in energy metabolites. In silico biodegradability pattern of Myricetin by BIOWIN TM models showed its rapid biodegradation under aerobic conditions and further emphasized its environmental sustainability. In conclusion, this is the first report spotlighting Myricetin as the active compound from M. maderaspatana and further suggests that it could serve as a potential anti-cancer agent for treating liver cancer.
PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITIES OF MUKIA MADERASPATANA VASANTHA KUMAR S., B. USHARANI International Journal of Applied Pharmaceutics, 2022 Objective: The study aimed to evaluate the bioactive compounds of Mukia maderaspatana and its properties. In addition, the preliminary phytochemical analysis and antioxidant potential of Mukia maderaspatana were performed. Methods: Extraction of leaves of Mukia maderaspatana made using Soxhlet apparatus, followed by evaluation of phytochemicals such as alkaloids, flavonoids, coumarin, glycosides, terpenoids, tannin, steroid, saponin, phenol, anthraquinone by the standard methodology of Sofowara, Trease and Evans, Harborne. Quantification of phytochemicals were performed accordingly to standard methodology. Antioxidant activity were performed accordingly to methodology of Shimada, Prieto, Zhang and Benzie. Methodology of Hema et al. is followed for detection of bioactive compounds by GC-MS analysis. Results: The Qualitative analysis revealed the presence of higher concentration of phytocompounds in Hydroalcoholic extract. The yield of alkaloid (215.8±4.78 mg AE), flavonoid (307.10±8.73 mg QE), phenol (374.50±6.41 mg GAE), tannin (129.98±5.32 mg TAE) and terpenoids (119.23±4.17 mg LE) are higher in hydroalcoholic extract than aqueous and methanolic extract. IC50 value for DPPH radical scavenging activity, Total antioxidant activity, Hydrogen peroxide scavenging activity and Ferric reducing antioxidant power activity are (61.43µg/ml), (59.68µg/ml), (55.69µg/ml), (64.60µg/ml). GC-MS analysis revealed the presence of about thirty compounds in hydroalcoholic extract of Mukia maderaspatana. Conclusion: The present study reveals that Mukia maderaspatana is an important medicinal plant. We can conclude that Leaves of Mukia maderaspatana are good source of antioxidants, which might cure the disease related to oxidative stress.
In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells Usharani B., Monisha R. Research Journal of Pharmacy and Technology, 2022 HeLa cells are broadly used for in-vitro studies in bringing out the medicinal value of plants against some dreadful diseases. From time immemorial, it is well proven that plant extracts play a vital role in treating many diseases including cancer. The plant extract contains copious varied phenolic compounds and flavonoids that are majorly responsible for its medicinal properties. In our study we have focused on the extraction of betel quid (betel leaves, areca nut and slaked lime in an appropriate proportion) using the solvent hexane. The hexane extract of the betel quid was evaluated for in vitro studies of apoptosis of HeLa cells. The results revealed that hexane extract of the betel quid at the maximum concentration of 200 µg/mL was very effective and only 20.89 % of cells were viable. The cytotoxic effect against HeLa cells was significant with IC50 of 96.33μg/ml. Further studies can be done on concentrating the bioactive compounds responsible for the anticancer activity. The bioactive active compounds can be formulated in the treatment of cancer in the upcoming research.
Identification of Bioactive Phytocomponents of Hydroalcoholic Extract of Enhalus acoroides by Gas Chromatography-Mass Spectrometry Analysis S. Navaith Ahmed, P. Kalaivani, P. Amudha, B. Usharani Research Journal of Pharmacy and Technology, 2021 Sea grass are good source of potent drug which has medicinal properties and able to cure human disease. One such marine plant is the Enhalus acoroides which belong to hydrocharitaeceae family. This marine species are widely distributed in the tropics of Indian and western Pacific Ocean and the species were collected from Ramanathapuram district for further process. This study reveals about the bioactive components present in Enhalus acoroides and identified its biological activity by Gas chromatography Mass spectrometry analysis using hydroalcoholic extract. The compounds present in Enhalus acoroides are Benzoic acid, 2- methyl 7- oxa bicyclol heptanes, 1, 3 Nonadiene, silane, ethoxytriethyl. Biological activities of the compounds present in the sample include antioxidant, hypocholesterolemic, antihypertensive, anti-inflammatory, anti-microbial, antiviral and anti-hepatotoxic effect.
Extraction of Essential oils from Cymbopogon citratus using Organic solvents Usharani B. Research Journal of Pharmacy and Technology, 2021 Essential oils are extracted from diverse plants and used in medicinal field for diverse conditions. The presence of unique aromatic compounds imparts aroma and medicinal values. Essential oil plays a vital role in pharmaceutical, cosmetics and also acts as rejuvenate. Though there are various plants from which essential oil are extracted, our study focuses on the extraction and comparison of the phytochemicals released from the fresh leaves of Cymbopogon citratus using two different organic solvents; (a). hexane (b) hexane and toluene in the ratio 1:1. Extraction was performed for 16 hours in Soxhlet extractor. The amount of oil obtained was weighed in both extractions and subjected to GC MS for phytochemical analysis. Results revealed that when the solvents hexane and toluene in the ratio 1:1 was used it gave a better yield of 3% from 5 gram of fresh leaves, while hexane yielded only 1.5%. The GC-MS analysis revealed that the compound released from both extractions are of diverse medicinal values and can be used in aromatherapy as rejuvenate. The compounds nerol (2,6 octadienal, 3,7 dimethyl.Z) and geraniol (2,6 octadienal, 3,7 dimethyl) are the common phytochemicals extracted .Nerol and geraniol are geometric isomers. Geraniol is reported as an efficient anticancer agent with additional properties of analgesic, antinflammatory and antiseptic values. Nerol is used as a fragrance. The compound 1 acetyl 4 hydroxy pyrrolidine 2 one is extracted when hexane and toluene were used for extraction in the ratio 1:1. It is reported to be anti-rheumatic and effective in the treatment of hips and joints. Hence it can be concluded that hexane and toluene in the ratio 1:1 can be used for extraction of oil considering its medicinal values and the increased % of extraction rather than using hexane alone.
Nanocomposite Clay Filter - A Cutting Edge Scenario In Disinfection Process Indian Journal of Environmental Protection, 2021
Constructed wetland: A sustainable approach pertaining to the treatment of wastewater convoying biodiversity An Introduction to Constructed Wetlands, 2020
Mukia maderaspatana (L.) M. Roem. phytoconstituents: Unveiling their anticancer potentials against hepatocellular carcinoma cells SV Kumar, C Shobana, D Rohini, J Manjunathan, R Ramasubburayan, ... South African Journal of Botany 169, 27-37 , 2024 2024.0 Citations: 8
Cyclotide Offers Neuroprotection Against 6-OHDA-Induced Toxicity in SH-SY5Y Neuroblastoma Cells by Downregulating mRNA Expression of MAO/α-Synuclein/LRRK2/PARK7/PINK1/PTEN Genes M Muniyasamy, U Boopathy, R Durairaj, S Chandrasekar Latin American Journal of Pharmacy 42 (3), 1396-1409 , 2023 2023.0
Albosteroid, A Steroidal Glycoside Derived From Morus Mongolica, Had A Protective Role In Preventing Nephrotoxicity In Rats Exposed To Paracetamol. GN Reddy, R Chauhan, M Muneeswari, D Arivukodi, N Niveditha, ... Journal of Pharmaceutical Negative Results 13 , 2022 2022.0
Chick Chorioallantois Membrane (CAM) Assay as an In Vivo Model to Study the Anti-angiogenesis and Anti-inflammatory activity of Nano-HSP. R Chauhan, GN Reddy, M Muneeswari, D Arivukodi, N Niveditha, ... Journal of Pharmaceutical Negative Results 13 , 2022 2022.0 Citations: 3
ANTIOXIDANT POTENTIAL AND SOME MEDICINAL PROPERTIES OF AGAR FORMULATIONS FROM SEAWEED Gracilaria filiforms IN COASTAL TAMIL NADU. S Chandrasekar, U Boopathy Journal of Pharmaceutical Negative Results 13 , 2022 2022.0 Citations: 1
Vitamin D in Physiological and Pathological Conditions C Shobana, B Usharani Thanuj International Publishers, Tamil Nadu, India, 90 , 2022 2022.0
Anticancer activity of hydroalcoholic extract of Enhalus acoroides N Ahmed, KS Vasantha, AK John, C Shobana, B Usharani International Journal of Health Sciences, 9528-9537 , 2022 2022.0 Citations: 7
Mycosynthesis of silver nanoparticles: Mechanism and applications J Annamalai, K Murugan, J Shanmugam, U Boopathy Green Synthesis of Silver Nanomaterials, 391-411 , 2022 2022.0 Citations: 4
In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells B Usharani, R Monisha Research Journal of Pharmacy and Technology 15 (1), 47-50 , 2022 2022.0 Citations: 3
Sewage treatment through constructed wetland system tailed by nanocomposite clay filter: a clean green initiative. B Usharania, N Vasudevanb 2021.0 Citations: 2
Identification of bioactive phytocomponents of hydroalcoholic extract of Enhalus acoroides by Gas Chromatography-Mass Spectrometry Analysis SN Ahmed, P Kalaivani, P Amudha, B Usharani Research Journal of Pharmacy and Technology 14 (12), 6511-6515 , 2021 2021.0 Citations: 5
Extraction of Essential oils from Cymbopogon citratus using Organic solvents B Usharani Research Journal of Pharmacy and Technology 14 (11), 5709-5712 , 2021 2021.0 Citations: 6
Pollutant removal from sewage in tropical climate by constructed wetland system: an asset for irrigation B Usharani, N Vasudevan International Letters of Natural Sciences 82 , 2021 2021.0 Citations: 2
Metagenomics study of the microbes in constructed wetland system treating sewage B Usharani International Letters of Natural Sciences 74 , 2019 2019.0 Citations: 27
Root exudates of Cyperus alternifolius in partial hydroponic condition under heavy metal stress B Usharani, N Vasudevan Pharmacognosy research 9 (3), 294 , 2017 2017.0 Citations: 15
Eco-friendly approach for leaching out heavy metals from sewage sludge B Usharani, N Vasudevan Chemistry and Ecology 32 (6), 507-519 , 2016 2016.0 Citations: 6
Impact of heavy metal toxicity and constructed wetland system as a tool in remediation B Usharani, N Vasudevan Archives of Environmental & Occupational Health 71 (2), 102-110 , 2016 2016.0 Citations: 18
Pharmaceutical Potential of Active Compounds From Plants: A Comprehensive Review on Alzhemier Disease and Future Possibilities M Muneeswari, GN Reddy, D Arivukodi, B Usharani, C Shobana International Journal of Health Sciences, 8553-8570 , 0
MOST CITED SCHOLAR PUBLICATIONS
Metagenomics study of the microbes in constructed wetland system treating sewage B Usharani International Letters of Natural Sciences 74 , 2019 2019.0 Citations: 27
Impact of heavy metal toxicity and constructed wetland system as a tool in remediation B Usharani, N Vasudevan Archives of Environmental & Occupational Health 71 (2), 102-110 , 2016 2016.0 Citations: 18
Root exudates of Cyperus alternifolius in partial hydroponic condition under heavy metal stress B Usharani, N Vasudevan Pharmacognosy research 9 (3), 294 , 2017 2017.0 Citations: 15
Mukia maderaspatana (L.) M. Roem. phytoconstituents: Unveiling their anticancer potentials against hepatocellular carcinoma cells SV Kumar, C Shobana, D Rohini, J Manjunathan, R Ramasubburayan, ... South African Journal of Botany 169, 27-37 , 2024 2024.0 Citations: 8
Anticancer activity of hydroalcoholic extract of Enhalus acoroides N Ahmed, KS Vasantha, AK John, C Shobana, B Usharani International Journal of Health Sciences, 9528-9537 , 2022 2022.0 Citations: 7
Extraction of Essential oils from Cymbopogon citratus using Organic solvents B Usharani Research Journal of Pharmacy and Technology 14 (11), 5709-5712 , 2021 2021.0 Citations: 6
Eco-friendly approach for leaching out heavy metals from sewage sludge B Usharani, N Vasudevan Chemistry and Ecology 32 (6), 507-519 , 2016 2016.0 Citations: 6
Identification of bioactive phytocomponents of hydroalcoholic extract of Enhalus acoroides by Gas Chromatography-Mass Spectrometry Analysis SN Ahmed, P Kalaivani, P Amudha, B Usharani Research Journal of Pharmacy and Technology 14 (12), 6511-6515 , 2021 2021.0 Citations: 5
Mycosynthesis of silver nanoparticles: Mechanism and applications J Annamalai, K Murugan, J Shanmugam, U Boopathy Green Synthesis of Silver Nanomaterials, 391-411 , 2022 2022.0 Citations: 4
Chick Chorioallantois Membrane (CAM) Assay as an In Vivo Model to Study the Anti-angiogenesis and Anti-inflammatory activity of Nano-HSP. R Chauhan, GN Reddy, M Muneeswari, D Arivukodi, N Niveditha, ... Journal of Pharmaceutical Negative Results 13 , 2022 2022.0 Citations: 3
In-vitro Cytotoxicity assay of Betel quid extract against HeLa cells B Usharani, R Monisha Research Journal of Pharmacy and Technology 15 (1), 47-50 , 2022 2022.0 Citations: 3
Sewage treatment through constructed wetland system tailed by nanocomposite clay filter: a clean green initiative. B Usharania, N Vasudevanb 2021.0 Citations: 2
Pollutant removal from sewage in tropical climate by constructed wetland system: an asset for irrigation B Usharani, N Vasudevan International Letters of Natural Sciences 82 , 2021 2021.0 Citations: 2
ANTIOXIDANT POTENTIAL AND SOME MEDICINAL PROPERTIES OF AGAR FORMULATIONS FROM SEAWEED Gracilaria filiforms IN COASTAL TAMIL NADU. S Chandrasekar, U Boopathy Journal of Pharmaceutical Negative Results 13 , 2022 2022.0 Citations: 1
Cyclotide Offers Neuroprotection Against 6-OHDA-Induced Toxicity in SH-SY5Y Neuroblastoma Cells by Downregulating mRNA Expression of MAO/α-Synuclein/LRRK2/PARK7/PINK1/PTEN Genes M Muniyasamy, U Boopathy, R Durairaj, S Chandrasekar Latin American Journal of Pharmacy 42 (3), 1396-1409 , 2023 2023.0
Albosteroid, A Steroidal Glycoside Derived From Morus Mongolica, Had A Protective Role In Preventing Nephrotoxicity In Rats Exposed To Paracetamol. GN Reddy, R Chauhan, M Muneeswari, D Arivukodi, N Niveditha, ... Journal of Pharmaceutical Negative Results 13 , 2022 2022.0
Vitamin D in Physiological and Pathological Conditions C Shobana, B Usharani Thanuj International Publishers, Tamil Nadu, India, 90 , 2022 2022.0
Pharmaceutical Potential of Active Compounds From Plants: A Comprehensive Review on Alzhemier Disease and Future Possibilities M Muneeswari, GN Reddy, D Arivukodi, B Usharani, C Shobana International Journal of Health Sciences, 8553-8570 , 0
