Rahul S Tade
@kavayitri bahinabai chaudhari north maharashtra university jalgaon
Assistant Professor
H. R. Patel Institute of Pharmaceutical Education and Research
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmaceutical Science, Cancer Research, Biomedical Engineering, Mechanics of Materials
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Krishna Kuna, Sudhakar Reddy Baddam, Sudhakar Kalagara, Pavan C. Akkiraju, Rahul S. Tade, and Sreenivas Enaganti
Elsevier BV
Rahul S. Tade, Swapnil N. Jain, Janhavi T. Satyavijay, Pratham N. Shah, Tejaswi D. Bari, Tanushri M. Patil, and Ruhi P. Shah
Tsinghua University Press
Debarati Maity, Rahul Tade, and Anagha S. Sabnis
Springer Science and Business Media LLC
Rahul Shankar Tade, Ashish Kalkal, and Pravin Onkar Patil
Springer Science and Business Media LLC
Zamir G. Khan, Minal R. Patil, Sopan N. Nangare, Ashwini G. Patil, Sai H. S. Boddu, Rahul S. Tade, and Pravin O. Patil
Springer Science and Business Media LLC
Rahul Shankar Tade and Pravin Onkar Patil
IOP Publishing
Abstract Lung cancer (LC) is a deadly malignancy that is posing a serious threat to human health. Therefore, early detection of LC biomarkers is the key to reducing LC-related fatalities. Herein, we present the first fluorescent-based selective detection of LC biomarker human telomerase reverse transcriptase (hTERT) using polyethyleneimine (PEI) functionalized graphene quantum dots (fGQDs). One-pot in situ synthesis of amine-functionalized GQDs was accomplished by hydrothermal carbonization of biowaste-derived cellulose and PEI. Synthesized fGQDs were characterized by various analytical techniques. Synthesized fGQDs not only exhibited enhanced fluorescence life-time but also excellent stability in the different solvents compared to bare GQDs. The surface activation of hTERT-Ab by carbodiimide chemistry (EDC-NHS) resulted in stacking interactions with fGQDs, involving adsorption-desorption as well as competitive mechanisms. The higher inherent affinity of hTERT-Ag (hTERT antigen) for hTERT-Ab (hTERT antibody) resulted in complex formation and recovery of fGQD fluorescence. As a result, this fluorescence sensing demonstrated a greater linear detection range (0.01 ng ml−1–100 μg ml−1) as well as a notable low detection limit (36.3 pg ml−1). Furthermore, the fabricated immunosensor (Ab@fGQDs) has excellent stability and performance in real samples, with an average recovery of 97.32%. The results of cytotoxicity and cellular bioimaging study in A549 cells show that fGQDs can be used for additional nanotherapeutics and biological applications.
Sopan Nangare, Prashant Patil, Ashwini Patil, Prashant Deshmukh, Trupti Powar, Jidnyasa Pantwalawalkar, Zamir Khan, Rahul Tade, Jayvadan K. Patel, and Pravin Patil
Springer International Publishing
Jidnyasa Pantwalawalkar, Sachin Chandankar, Rahul Tade, Zamir Khan, Matin Shaikh, Trupti Powar, Prashant Patil, Vrushabh Sugandhi, and Sopan Nangare
IOP Publishing
Abstract Prostate cancer (PC) is significantly increasing as a principal cause of mortality among the men’s population. Accordingly, research scholars have made tremendous development for the effective treatment of PC. Despite this, the early diagnosis of PC is a challenging task for scientific fraternities due to the plenty of demerits of currently engaged methods in terms of sensitivity, selectivity, and many more. For that reason, PC management needs exceptional alternative strategies for proficient management of PC that can endow with early diagnosis of PC and eventually it will result in promising cure of PC. Strategies involved in advanced nanomaterials especially carbon nanomaterials have gained tremendous consideration due to their numerous benefits. Notably, graphene quantum dots (GQDs) are a promising next-generation carbon nanomaterial with manifold biomedical applications such as biosensing and drug delivery in cancer and different life-threatening health issues that may be due to the versatile and tunable properties of GQDs. In a nutshell, it provides high sensitivity and selectivity towards the target biomarker of PC. In this review, the prevalence and distribution of PC, major highlights and limitations of current diagnostics and treatments, as well as recent trends for PC management have been discussed in brief. Afterward, the synthesis methods, properties, biocompatibility, and bioconjugation of GQDs along with their impact on the performance of biosensors have been summarised in brief. Finally, the application of GQD based biosensors exhibited the lowest detection limit up to femtogram in simulated and real-time samples. As a result, the applications of GQDs as novel biosensors presents plentiful advantages including high sensitivity, high selectivity, high stability, rapid response, etc of the PC biomarkers. Therefore, this review will provide the details of recent updates to prepare and extend advanced biosensing prospects using GQDs as excellent nanomaterial probes in PC and other cancer types.
Rahul Shankar Tade and Pravin Onkar Patil
American Chemical Society (ACS)
The diagnosis of tumor biomarkers is an attentive approach for the early detection and treatment of cancer. However, a cost-effective, simple, rapid, selective, and sensitive method is a basic prerequisite for diagnostic research. Herein, we present a novel fluorescence-based label-free sensing strategy for the sensitive and selective detection of carcinoembryonic antigen (CEA) using poly-l-lysine (PLL)-functionalized graphene quantum dots (GQDs). The GQDs were synthesized using a greener method by employing carbonized peanut shell (PNS) waste as a carbon source, and functionalization was accomplished using PLL (PLL-GQDs). The fluorescence stability of the PLL-GQDs was tested in a variety of solvent systems and pH solutions. When compared to nonfunctionalized GQDs (PNS-GQDs), prepared PLL-GQDs demonstrated increased fluorescence lifetime, high quantum yield, excellent photostability, biocompatibility, and greater cellular uptake. The PLL-GQDs with abundant surface amine and carboxylic groups showed selective interactions with an activated CEA antibody (CEA-Ab), resulting in the quenching of fluorescence signals. Because of the strong bioaffinity of CEA to the CEA-Ab, the antibody was unwrapped, resulting in the formation of an antibody-antigen complex and the recovery of fluorescence. As a result of this relationship, a turn "on-off-on" sensing mechanism with a strong response to CEA concentration (0.01 ng mL-1 to 100 μg mL-1) and a detection limit of 1.19 pg mL-1 was demonstrated. Furthermore, the fabricated CEA immunosensor (CEA-Ab@PLL-GQDs) performed admirably in real sample analysis, with an average recovery of 98.32%. The cellular uptake performance of PLL-GQDs was also demonstrated in the A427 cell lines, exhibiting a greater cellular uptake potential than PNS-GQDs. The cellular bioimaging study demonstrates that PLL-GQDs can be used for additional therapeutic and biological applications.
Rahul S. Tade, Mahesh P. More, Sopan N. Nangare, and Pravin O. Patil
Informa UK Limited
Abstract Lung cancer (LC) is heading up as a substantial cause of mortality worldwide. Despite enormous progress in cancer management, LC remains a crucial problem for oncologists due to the lack of early diagnosis and precise treatment. In this context, numerous early diagnosis and treatment approaches for LC at the cellular level have been developed using advanced nanomaterials in the last decades. Amongst this, graphene quantum dots (GQDs) as a novel fluorescent material overwhelmed the horizons of materials science and biomedical fields due to their multifunctional attributes. Considering the complex nature of LC, emerging diagnostic and therapeutic (Theranostics) strategies using GQDs proved to be an effective way for the current practice in LC. In this line, we have abridged various approaches used in the LC theranostics using GQDs and its surface-engineered motif. The admirable photophysical attributes of GQDs realised in photolytic therapy (PLT), hyperthermia therapy (HTT), and drug delivery have been discussed. Furthermore, we have engrossed the impasse and its effects on the use of GQDs in cancer treatments from cellular level (in vivo-in vitro) to clinical. Inclusively, this review will be an embodiment for the scientific fraternity to design and magnify their view for the theranostic application of GQDs in LC treatment.
Sopan N. Nangare, Premnath M. Sangale, Ashwini G. Patil, Sai HS. Boddu, Prashant K. Deshmukh, Namdeo R. Jadhav, Rahul S. Tade, Dilip R. Patil, Abhijeet Pandey, Srinivas Mutalik,et al.
Elsevier BV
S N Nangare, S Landge, A G Patil, R S Tade, P K Deshmukh, and P O Patil
IOP Publishing
The green synthesis of iron nanoparticles (FeNPs) doped and silver nanoparticles (AgNPs) loaded reduced graphene oxide (rGO) (Fe-Ag@rGO) nanocomposite and its applications in methylene blue (MB), malachite green (MG), rhodamine B (RB) degradation were reported. Initially, AgNPs loaded rGO (Ag@rGO) nanocomposites were synthesised simultaneously by an ecological method using Tamarindus indica shell extract as a green reducing agent. Then, the doping of FeNPs into rGO@Ag nanocomposites afforded Fe-Ag@rGO nanocomposite. Interestingly, the finding of this study confirmed that the Fe-Ag@rGO nanocomposites exhibited countless stupendous features in terms of dye degradation. Briefly, the UV-visible spectroscopy and Fourier-transform infrared spectroscopy (FTIR) study confirmed the synthesis of Fe-Ag@rGO nanocomposite. The scanning electron microscopy (SEM) images showed the spherical shape with cross-linked network structures that confirmed the surface modification and synthesis of Fe-Ag@rGO nanocomposite. Finally, the dye degradation potential of the photocatalyst was found to be 97.20%, 98.43%, and 97.33%, for MB, MG, RB, respectively. Herein, the improved photocatalytic performance of the Fe-Ag@rGO was found due to the larger surface area, porous nature, high electron mobility, and synergistic effect of the Fe-Ag@rGO nanocomposite. Additionally, the effective interfacial hybridisation of ‘Ag’, and doping of ‘Fe’ on the rGO sheet extended the duration of the photogenerated electron (e-) hole pairs that can also be contributing to dye degradation. Conclusively, the present experiment provides the new Fe-Ag@rGO nanocomposite to the dye degradation, which could be improved environmental remediation.
Rahul Shankar Tade, Sopan Namdev Nangare, and Pravin Onkar Patil
IOP Publishing
Abstract The worldwide frontiers of research have experienced a flood of developments in advanced nanomaterials. Among these, graphene, a member of the carbon family, has now replaced many traditional materials and broadened the horizons of material chemistry, analytical chemistry, pharmaceutics, and other multidisciplinary fields. Owing to the exceptional properties of graphene, it has been widely utilized in various nanocomposites as a reinforcing material and for biosensing components. The present review serves as a familiarization for budding researchers in the materials science and analytical fields, where the use of graphene in biosensing-related applications had long been foreseen. Furthermore, we also offer a brief review of graphene’s tunable properties for biosensing. This article describes the actual mechanisms of interfaces that interact with graphene, such as immunogenic agents, bacteria, and other biomolecules. We also discuss the application of graphene-based materials to the biosensing of a range of analytes, and the challenges and future perspectives of graphene. Thus, this review gives a detailed insight into biosensing with graphene, graphene’s fundamental properties, and application perspectives.
Rahul S Tade, Pravin O Patil, and Vivekanand K Chatap
IOP Publishing
Abstract Cellulose-based magnetic nanocomposites (Cf-MNCs) have been introduced using a modified one-pot in situ co-precipitation method using iron salts with various concentrations in the alkali solution. Fabricated nanocomposites investigated for structural and functional properties with different spectroscopic characterization techniques prior to use in dye degradation study. The scanning electron microscopy revealed the morphological structure of the synthesized nanofibers and nanocomposites. The elemental analysis and vibrating sample magnetometry emphasized the presence of Fe elements attributed to the iron salts. The HRTEM analysis showed a destructed cellulose fiber network indicating its arrangement into nanocomposites. Moreover, the crystal properties of the Cf-MNCs were accomplished using x-ray powder diffraction (79.3% crystallinity). The Fourier transform infrared analysis and differential scanning calorimetry gives the idea about the structural and functional changes in the cellulose fibers loaded with iron oxide nanoparticles. The functional adsorption properties of the prepared nanocomposites have been evaluated using methylene blue and Alizarin red S carcinogenic dyes. The dye adsorption of the fabricated Cf-MNCs nanocomposites was found to be 93%. We affirmed that this novel eco-friendly degradable polymer-based nanocomposite has great potential in the field of catalyst fabrication for the degradation of organic pollutants in wastewater.
Sopan Nangare, Shailesh Dugam, Pravin Patil, Rahul Tade, and Namdeo Jadhav
IOP Publishing
Abstract Amongst assorted regio-selective and targeted oral drug delivery strategies accepted for the gastro-retentive drug delivery system (GRDDS), the floating drug delivery system (FDDS) holds a major share as clinically accepted formulations. The major objective of the present investigation was to explore the silk industry waste protein, silk fibroin (SF) as a possible electrospun nanocarrier for the FDDS. In a nutshell, electrospinning (ES) is one of the flexible and astonishing strategies for the fabrication of porous electrospun nanofibers (NFs), which offers the potential to amend the floating profile, dissolution rate, solubility, and release patterns of the drug, etc as per compendial requirements. Looking at the prospects of floating SF-NFs preparation, we have isolated and lyophilized the SF from industrial waste cocoons and prepared drug-loaded SF single polymer nanofibers (SPN). Lafutidine (LF) being a good candidate for GRDDS selected as a model drug, which is an excellent proton pump inhibitor, mainly used in the treatment of gastric ulcers. Finally, the obtained LF loaded SF-NFs (LF-SF-NFs) were successfully analyzed for physicochemical characteristics, porosity, swelling index, antioxidant activity, mucoadhesion strength, floating properties, enzymatic degradation, and accelerated stability study, etc. Further, these LF-SF-NFs were evaluated for percent drug content, weight variation, in-vitro dissolution in 0.1 N hydrochloric acid (HCl, pH:1.2) and fasted state simulated gastric fluid (FSSGF), and accelerated stability study. It has shown significant floating time >18 h, about 99% ± 0.58% floating buoyancy with sustained release up to 24 h. LF-SF-NFs showed good compatibility, entrapment efficiency, antioxidant activity, mucoadhesion strength, enzymatic degradation, and long term stability. Soon, the essential floating and drug release profiles can claim single polymer (SF) based electrospun protein NFs as a possible novel oral nanocarrier for FDDS.
Sopan N. Nangare, Sayali R. Patil, Ashwini G. Patil, Zamir G. Khan, Prashant K. Deshmukh, Rahul S. Tade, Mahendra R. Mahajan, Sanjaykumar B. Bari, and Pravin O. Patil
Springer Science and Business Media LLC
Pravin Onkar Patil, Sopan Namdev Nangare, Pratiksha Pramod Patil, Ashwini Ghanashyam Patil, Dilip Ramsing Patil, Rahul Shankar Tade, Arun Madhukar Patil, Prashant Krishnarao Deshmukh, and Sanjay Baburao Bari
Tsinghua University Press
Rahul S. Tade and Pravin O. Patil
American Chemical Society (ACS)
Breast cancer (BC) is increasing as a significant cause of mortality among women. In this context, early diagnosis and treatment strategies for BC are being developed by researchers at the cellular level using advanced nanomaterials. However, immaculate etiquette is the prerequisite for their implementation in clinical practice. Considering the stolid nature of cancer, combining diagnosis and therapy (theranostics) using graphene quantum dots (GQDs) is a prime focus and challenge for researchers. In a nutshell, GQDs is a new shining star among various fluorescent materials, which has acclaimed fame in a short duration in materials science and the biomedical field as well. From this perspective, we review various strategies in BC treatment using GQDs alone or in combination. In addition, the photophysical properties of GQDs explored in photothermal therapy, hyperthermia therapy, and photodynamic therapy are also discussed. Moreover, we also focus on the strategic use of GQDs both as drug carriers and as combinatorial-guided drug delivery motifs. This Review provides an update for the scientific community to plan and expand advanced theranostic horizons in BC using GQDs.
Rahul S. Tade and Pravin O. Patil
Elsevier BV
M. Shitole, S. Dugam, R. Tade, and S. Nangare
Elsevier BV
Rahul S Tade, Sopan N Nangare, Ashwini G Patil, Abhieet Pandey, Prashant K Deshmukh, Dilip R Patil, Tanisha N Agrawal, Srinivas Mutalik, Arun M Patil, Mahesh P More,et al.
IOP Publishing
Graphene quantum dots (GQDs), impressive materials with enormous future potential, are reviewed from their inception, including different precursors. Considering the increasing burden of industrial and ecological bio-waste, there is an urgency to develop techniques which will convert biowaste into active moieties of interest. Amongst the various materials explored, we selectively highlight the use of potential carbon containing bioprecursors (e.g. plant-based, amino acids, carbohydrates), and industrial waste and its conversion into GQDs with negligible use of chemicals. This review focuses on the effects of different processing parameters that affect the properties of GQDs, including the surface functionalization, paradigmatic characterization, toxicity and biocompatibility issues of bioprecursor derived GQDs. This review also examines current challenges and s the ongoing exploration of potential bioprecursors for ecofriendly GQD synthesis for future applications. This review sheds further light on the electronic and optical properties of GQDs along with the effects of doping on the same. This review may aid in future design approaches and applications of GQDs in the biomedical and materials design fields.
Rahul Shankar Tade, Sopan Namdev Nangare, and Pravin Onkar Patil
Tsinghua University Press
Pravin O. Patil, Gaurav R. Pandey, Ashwini G. Patil, Vivek B. Borse, Prashant K. Deshmukh, Dilip R. Patil, Rahul S. Tade, Sopan N. Nangare, Zamir G. Khan, Arun M. Patil,et al.
Elsevier BV
Rahul S Tade, Mahesh P More, Vivekanand K Chatap, Pravin O Patil, and Prashant K Deshmukh
IOP Publishing
In the present study, one pot fabrication process was used for the synthesis of magnetic nanocellulose fiber composite (MCFNCs), wherein In situ co-precipitation methodology contains reaction of iron with ammonia in controlled temperature environment and cellulose fibers acts as a capping and stabilizing agent. The crosslinked cellulose fibers helps to dissociate Fe ions and avoid aggregation during the nucleation stages. The Nystatin (Nyst) was used as a model drug for studying the release characteristics of MCFNCs. The preliminary confirmation was done by comparing the FTIR spectra of synthesized MCFNCs with its precursor. The fabricated MCFNCs was characterized by x-ray Diffraction, Vibrating Sample Magnetometry, Particle Size, etc. The surface morphology and internal structure were identified by Scanning and Transmission Electron Microscopic observation of respective samples. It shows porous aggregated structure of synthesized nanocellulose, BET surface area and BJH pore size was determined simultaneously and found to be 13.42 m2 g−1 and 104.48 Å respectively. Due to the porous nature of nanocellulose fiber, it has high loading capacity i.e. around 17.8% amongst porous material category. In vitro drug release characteristics of Nyst loaded MCFNCs compared to pure drug showed sustained delivery for up to 8h time period. The Antifungal activity was evaluated on Candida Albicans and showed prominent inhibitory activity. The biocompatible nature of synthesized nanocomposites obtained from the natural nanocellulose fiber has a huge prospective in magnetically guided drug delivery in various parasitic diseases and have a potential of biomedical applications.