Pragyan Paramita
@the ohio state university
Postdoctoral Research Fellow
EDUCATION
I have pursued my doctorate in the field of Bone Tissue Engineering from the department of Medical Biotechnology, Chettinad Academy of Research and Education, India.
RESEARCH, TEACHING, or OTHER INTERESTS
Biomaterials, Biomedical Engineering, Orthopedics and Sports Medicine, Multidisciplinary
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Pragyan Paramita, Sai Nivethitha Sethu, Namasivayam Subhapradha, Vijayashree Ragavan, Ramachandran Ilangovan, Anandan Balakrishnan, Narasimhan Srinivasan, Ramachandran Murugesan, and Ambigapathi Moorthi
Informa UK Limited
Abstract Understanding the mechanism behind neuronal regeneration is critical for treating ischemic stroke and traumatic brain injury. The presence of neural stem cells in and around the sub-ventricular zone of human and also in zebrafish is evidenced. In this current study, the neuro-protective potential of nano-formulated hesperetin on injury-induced neurogenesis in zebrafish was assessed. Nanoformulation of hesperetin was prepared by anti-solvent precipitation technique using sodium dodecyl sulfate (SDS) as the stabilizing agent. The synthesized particles were characterized using SEM, DLS, XRD and FT-IR. Anti-oxidant capacity of nano hesperetin (nHST) in in vitro followed by in vivo studies in a traumatic brain injury (TBI) model of adult zebrafish (Danio rerio), catalase activity, histological analysis and gene expression studies for the genes Sox2, Nestin, Fabp7a and HuC were carried out. The synthesized particles were found to be in nanoscale and SDS had successfully integrated with hesperetin. Moreover, nHST had a significantly higher anti-oxidant capacity in vitro. Catalase levels in nHST treated group were significantly restored compared to other groups. Histological studies supported reduced tissue damage on oral administration of nano-hesperetin as compared to other groups. Gene expression studies showed that nano-hesperetin at a concentration of 10 μM when administered orally induced proliferation of neural stem cells without inducing cell death.
Mohamed Abudhahir, Azeena Saleem, Pragyan Paramita, Sukumar Dinesh Kumar, Chung Tze‐Wen, Nagarajan Selvamurugan, and Ambigapathi Moorthi
Wiley
The bone defects healing are always associated with post implantation infections; hence biomaterials rules significant role for orchestration of defective bone. In this study, we synthesized biocomposite scaffold by combining polycaprolactone (PCL), wollastonite (Ws) and metal ions (Cu) by electrospinning technique. The manufactured scaffolds (PCL/Ws andPCL/Cu-Ws) were subjected to physio-chemical characterization by scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier Transform Infra Red Spectroscopy (FTIR) and XRD. The surface topography of the scaffolds was found to be micro-fibrous in nature and each fiber was cylindrical in structure. The exogenous biomineralization and protein adsorption capacity of these scaffolds were studied. Enhanced amount of protein was adsorbed on PCL/Cu-Ws than PCL/Ws scaffold after incubating for 48 hr in foetal bovine serum (FBS) also the biomineralization shown to be promoted the apatite formation in vitro. The synthesized PCL/Cu-Ws scaffold was biocompatible to mouse mesenchymal stem cells and enhanced the mRNA expressionof osteoblastic specific marker genes including alkaline phosphatase and type I collagen and major transcription factor Runx2 in the presence of osteogenic medium indicates the osteoconductive nature of the scaffolds. The amount of calcium deposition and promotion of osteoblast differentiation and mineralization on human osteoblast cells was confirmed by alizarin red staining. The fabricated scaffolds possess potent antibacterial effect against Staphylococcu aureus and Escherichia coli. Hence, our outcomes confirmed that the PCL/Ws and PCL/Cu-Ws scaffolds promote bonesynthesis by cell proliferation and differentiation suitable for applications in bone regeneration orbone defects.
Pragyan Paramita, Murugesan Ramachandran, Srinivasan Narashiman, Selvamurugan Nagarajan, Dinesh Kumar Sukumar, Tze-Wen Chung, and Moorthi Ambigapathi
Springer Science and Business Media LLC
AbstractBone is a flexible and electro active tissue that is vulnerable to various traumatic injuries. The self-healing of damaged bone tissue towards reconstruction is limited due to the lack of proper niche compliances. Nevertheless, the classical grafting techniques like autograft/allograft for bone repair pose challenges like bacterial infections and donor-site morbidity with unsatisfactory outcomes. The use of appropriate biomaterial with osteogenic potential can meet these challenges. In this regard, bioactive glass ceramics is widely used as a bone filler or graft material because of its bonding affinity to bone leading towards bone reconstruction applications without the challenge of post implant infections. Hence, the current study is aimed at addressing this potentiality of zinc (Zn) for doped the bioglass at nano-scale advantages for bone tissue repair. Since, Zn has been demonstrated to have not only antibacterial property but also the stimulatory effect on osteoblasts differentiation, mineralization by enhancing the osteogenic genes expression. In view of these, the present study is focused on sol–gel synthesis and pysico-chemical characterization of Zinc-doped bioglass nanoparticles (Zn-nBGC) and also analyzing its biological implications. The surface morphological and physiochemical characterizations using SEM, EDX, FT-IR and XRD analysis has shown the increased surface area of Zn-nBGC particles providing a great platform for biomolecular interaction, cytocompatibility, cell proliferation and osteogenic differentiation. The obtaining hydroxy apatite groups have initiated in vitro mineralization towards osteogenic lineage formation. Zn has not only involved in enhancing cellular actions but also strengthen the ceramic nanoparticles towards antibacterial application. Hence the finding suggests a biomaterial synthesis of better biomaterial for bone tissue engineering application by preventing post-operative bacterial infection.
Pragyan Paramita, Vimala Devi Subramaniam, Ramachandran Murugesan, Madhumala Gopinath, Ilangovan Ramachandran, Satish Ramalingam, Xiao Feng Sun, Antara Banerjee, Francesco Marotta, and Surajit Pathak
Institution of Engineering and Technology (IET)
Research dealing with early diagnosis and efficient treatment in colon cancer to improve patient's survival is still under investigation. Chemotherapeutic agent result in high systemic toxicity due to their non-specific actions on DNA repair and/or cell replication. Traditional medicine such as Lycopodium clavatum (LC) has been claimed to have therapeutic potentials against cancer. The present study focuses on targeted drug delivery of cationic liposomal nanoformulated LC (CL-LC) in colon cancer cells (HCT15) and comparing the efficacy with an anti-colon cancer drug, 7-ethyl-10-hydroxy-camptothecin (SN38) along with its nanoformulated form (CL-SN38). The colloidal suspension of LC was made using thin film hydration method. The drugs were characterised using ultraviolet, dynamic light scattering, scanning electron microscopy, energy, dispersive X-ray spectroscopy. Invitro drug release showed kinetics of 49 and 89% of SN38 and LC, whereas CL-SN38 and CL-LC showed 73 and 74% of sustained drug release, respectively. Studies on morphological changes, cell viability, cytotoxicity, apoptosis, cancer-associated gene expression analysis of Bcl-2, Bax, p53 by real-time polymerase chain reaction and western blot analysis of Bad and p53 protein were performed. Nanoformulated LC significantly inhibited growth and increased the apoptosis of colon cancer cells indicating its potential anti-cancer activity against colon cancer cells.
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MOST CITED SCHOLAR PUBLICATIONS
GRANT DETAILS
I have been awarded with CSIR-SRF (Direct) Extramural Research Fund for 2 years (2019-2021) during my PhD.
INDUSTRY EXPERIENCE
I had an opportunity to work with TATA ELXSI, Bangaluru- INDIA, as a Medical Device Specialist for a year after my PhD. The work was relevant to evaluate and write the Clinical outputs of a Bone implant (Hip/Knee)- Medical devices from well-known international manufacturers.