Sunilkumar Bhavsar
@vnsgu.ac.in
Veer Narmad South Gujarat University
Veer Narmad South Gujarat University
RESEARCH, TEACHING, or OTHER INTERESTS
Biochemistry, Genetics and Molecular Biology, Biotechnology, Agricultural and Biological Sciences, Spectroscopy
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Sunil Bhavsar, Pravin Dudhagara, and Shantilal Tank
Public Library of Science (PLoS)
The thermophilic bacterium, Bacillus licheniformis U1 is used for the optimization of bacterial growth (R1), laccase production (R2) and synthetic disperse blue DBR textile dye decolorization (R3) in the present study. Preliminary optimization has been performed by one variable at time (OVAT) approach using four media components viz., dye concentration, copper sulphate concentration, pH, and inoculum size. Based on OVAT result further statistical optimization of R1, R2 and R3 performed by Box–Behnken design (BBD) using response surface methodology (RSM) in R software with R Commander package. The total 29 experimental runs conducted in the experimental design study towards the construction of a quadratic model. The model indicated that dye concentration 110 ppm, copper sulphate 0.2 mM, pH 7.5 and inoculum size 6% v/v were found to be optimum to maximize the laccase production and bacterial growth. Whereas, maximum dye decolorization achieved in media containing dye concentration 110 ppm, copper sulphate 0.6 mM, pH 6 and inoculum size 6% v/v. R package predicted R2 of R1, R2 and R3 were 0.9917, 0.9831 and 0.9703 respectively; likened to Design-Expert (Stat-Ease) (DOE) predicted R2 of R1, R2, and R3 were 0.9893, 0.9822 and 0.8442 respectively. The values obtained by R software were more precise, reliable and reproducible, compared to the DOE model. The laccase production was 1.80 fold increased, and 2.24 fold enhancement in dye decolorization was achieved using optimized medium than initial experiments. Moreover, the laccase-treated sample demonstrated the less cytotoxic effect on L132 and MCF-7 cell lines compared to untreated sample using MTT assay. Higher cell viability and lower cytotoxicity observed in a laccase-treated sample suggest the impending application of bacterial laccase in the reduction of toxicity of dye to design rapid biodegradation process.
Pravin Dudhagara, Anjana Ghelani, Sunil Bhavsar, and Shreyas Bhatt
Elsevier BV
Sunil Bhavsar, Twinkle Solanki, Suchita Amin, and Neeru Jain
Science Publications
Assessment of hybrid seed genetic purity is one of the most important quality control parameters in hybrid seed production. With the objective of replacing the GOT with DNA based assays, Cytoplasmic Male Sterile (CMS), restorer and hybrid lines have been screened by means of microsatellite and Sequence Tagged Site (STS) markers. Cytoplasmic Male Sterile (CMS) lines often get contaminated with cognate isonuclear maintainer lines during multiplication. The PCR assay was able to detect precisely the impurities in a commercial seed lot of CMS line. The extent of heterozygosity within parental lines of rice hybrids were assessed and the results suggest that a single, appropriately chosen microsatellite marker should be sufficient for assessing hybrid seed purity.
Pravin Dudhagara, Sunil Bhavsar, Chintan Bhagat, Anjana Ghelani, Shreyas Bhatt, and Rajesh Patel
Elsevier BV