Ana Maria Villa Manso
@uam.es
Scopus Publications
- Combination of few-layer bismuthene and glutamate dehydrogenase for enhanced glutamate biosensing
Ana María Villa-Manso, Iñigo Torres, Félix Pariente, Encarnación Lorenzo, Félix Zamora, Eva Mateo-Martí, Cristina Gutiérrez-Sánchez, Mónica Revenga-Parra
Microchemical Journal, 2026
This work reports the development of an enzymatic electrochemical biosensor for glutamate detection based on few-layer bismuthene hexagons (FLB), a two-dimensional nanomaterial with outstanding electrocatalytic and biocompatible properties. FLB was synthesized by a wet chemistry method and integrated into screen-printed graphene electrodes (SPGrE) to provide a stable and biocompatible conductive surface for the immobilization of redox enzyme glutamate dehydrogenase (GLDH). The combination of FLB with GLDH enabled the efficient electrochemical detection of glutamate through the oxidation of enzymatically generated NADH. The resulting nanostructured biosensing platform exhibits a wide linear detection range (6.54–125 μM), a low detection limit (1.96 μM), and high selectivity toward common interfering compounds. Each step of the bioplatform fabrication was characterized using electrochemical, microscopic and spectroscopic techniques, confirming the successful immobilization of FLB and GLDH. The applicability of the biosensor was further validated by glutamate determination in human serum and food samples, demonstrating excellent analytical performance and storage stability over 20 days. These results highlight the potential of FLB as a key nanomaterial for the development of highly sensitive and selective enzymatic electrochemical biosensors. - Graphene oxide membranes with amine intercalation for dye recovery from textile effluents
Raul Pla, Jose Alberto Baeza, Manuel Fernández-Marquez, Gabriela Soledad Live Lozada, Ana M. Villa-Manso, Mónica Revenga-Parra, Luisa Calvo, Noelia Alonso-Morales, Miguel Angel Gilarranz
Journal of Industrial and Engineering Chemistry, 2025
The textile industry generates significant amounts of wastewater, with dyes and salts as primary contaminants. One method to valorize this wastewater is by separating dyes, enabling the reuse of both dye and water. This study focuses on developing graphene oxide (GO) membranes modified with various amines and solvent media to achieve selective separation. Among the membrane tested, those prepared using 1,3-diaminopropane exhibited excellent performance, achieving high dye rejection (>97 %) and low salt rejection (32 %) while maintaining good permeability. These findings highlighting the potential of GO membranes for improving dye recovery and reuse. - Bifunctional Au@Pt/Au nanoparticles as electrochemiluminescence signaling probes for SARS-CoV-2 detection
Ana M. Villa-Manso, Tamara Guerrero-Esteban, Félix Pariente, Celia Toyos-Rodríguez, Alfredo de la Escosura-Muñiz, Mónica Revenga-Parra, Cristina Gutiérrez-Sánchez, Encarnación Lorenzo
Talanta, 2023
A novel immunosensor based on electrochemiluminescence resonance energy transfer (ECL-RET) for the sensitive determination of N protein of the SARS-CoV-2 coronavirus is described. For this purpose, bifunctional [email protected] nanoparticles composed of a Pt-coated Au core and finally decorated with small Au inlays ([email protected]/Au NPs) have been synthesized to act as ECL acceptor, using [Ru (bpy)3]2+ as ECL donor. These nanoparticles are efficient signaling probes in the immunosensor developed. The proposed ECL-RET immunosensor has a wide linear response to the concentration of N protein of the SARS-CoV-2 coronavirus with a detection limit of 1.27 pg/mL. Moreover, it has a high stability and shows no response to other proteins related to different virus. The immunosensor has achieved the quantification of N protein of the SARS-CoV-2 coronavirus in saliva samples. Results are consistent with those provided by a commercial colorimetric ELISA kit. Therefore, the developed immunosensor provides a feasible and reliable tool for early and effective detection of the virus to protect the population. - Azure A embedded in carbon dots as NADH electrocatalyst: Development of a glutamate electrochemical biosensor
Emiliano Martínez-Periñán, Aitor Domínguez-Saldaña, Ana M. Villa-Manso, Cristina Gutiérrez-Sánchez, Mónica Revenga-Parra, Eva Mateo-Martí, Félix Pariente, Encarnación Lorenzo
Sensors and Actuators B Chemical, 2023
Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. A deep characterization of AA-CDs nanomaterial has been carried out, proving the covalent attachment of azure A molecules into the carbon dots nanostructure. Disposable screen-printed carbon electrodes (SPCE) have been modified with AA-CDs, through the action of chitosan polymer (Chit-AA-CDs/SPCE). The Chit-AA-CDs/SPCE electrocatalytic activity towards the oxidation of NADH has been proved, obtaining excellent results regarding the low oxidation potential achieved (−0.15 V vs. Ag) and low detection and quantification limits (LOD and LOQ) for NADH, 16 and 53 µM, respectively. The developed electrochemical platform has been applied for the construction of a glutamate biosensor by immobilizing L-glutamic dehydrogenase (GLDH/Chit-AA-CDs/SPCE). The morphology of GLDH/Chit-AA-CDs/SPCE platform was analysed by AFM at each different step of the electrode modification process. The resulting biosensing platform is capable of detect NADH enzymatically generated by GLDH in the presence of glutamate and NAD+. Good analytical parameters were obtained for glutamate analysis using GLDH/Chit-AA-CDs/SPCE, as LOD and LOQ of 3.3 and 11 µM, respectively. The biosensor has been successfully applied to the analysis of food and biological samples. - Sensitive SARS-CoV-2 detection in wastewaters using a carbon nanodot-amplified electrochemiluminescence immunosensor
Tamara Guerrero-Esteban, Cristina Gutiérrez-Sánchez, Ana M. Villa-Manso, Mónica Revenga-Parra, Félix Pariente, Encarnación Lorenzo
Talanta, 2022
Given the great utility that having fast, efficient and cost-effective methods for the detection of SARS-CoV-2 in wastewater can have in controlling the pandemic caused by this virus, the development of new dependable and specific SARS-CoV-2 coronavirus sensing devices to be applied to wastewater is essential to promote public health interventions. Therefore, herein we propose a new method to detect SARS-CoV-2 in wastewater based on a carbon nanodots-amplified electrochemiluminescence immunosensor for the determination of the SARS-CoV-2 Spike S1 protein. For the construction of the immunosensor, N-rich carbon nanodots have been synthetized with a double function: to contribute as amplifiers of the electrochemiluminescent signal in presence of [Ru(bpy)3]2+ and as antibody supports by providing functional groups capable of covalently interacting with the SARS-CoV-2 Spike S1 antibody. The proposed ECL immunosensor has demonstrated a high specificity in presence of other virus-related proteins and responded linearly to SARS-CoV-2 Spike S1 concentration over a wide range with a limit of detection of 1.2 pg/mL. The immunosensor has an excellent stability and achieved the detection of SARS-CoV-2 Spike S1 in river and urban wastewater, which supplies a feasible and reliable sensing platform for early virus detection and therefore to protect the population. The detection of SARS-CoV-2 Spike S1 in urban wastewater can be used as a tool to measure the circulation of the virus in the population and to detect a possible resurgence of COVID-19. - Preparation of high-quality few-layers bismuthene hexagons
Iñigo Torres, Ana María Villa-Manso, Mónica Revenga-Parra, Cristina Gutiérrez-Sánchez, Diego A. Aldave, Elena Salagre, Enrique García Michel, María Varela, Julio Gómez-Herrero, Encarnación Lorenzo, Félix Pariente, Félix Zamora
Applied Materials Today, 2022 - 2D MoS2 nanosheets and hematein complexes deposited on screen-printed graphene electrodes as an efficient electrocatalytic sensor for detecting hydrazine
Ana M. Villa-Manso, Mónica Revenga-Parra, Mariano Vera-Hidalgo, Manuel Vázquez Sulleiro, Emilio M. Pérez, Encarnación Lorenzo, Félix Pariente
Sensors and Actuators B Chemical, 2021 - Bioelectrocatalytic platforms based on chemically modified nanodiamonds by diazonium salt chemistry
Mónica Revenga-Parra, Ana M. Villa-Manso, María Briones, Eva Mateo-Martí, Emiliano Martínez-Periñán, Encarnación Lorenzo, Félix Pariente
Electrochimica Acta, 2020
